BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,024,983	C→G	90.4%	G86G (GGC→GGG)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,024,983	0	C	G	90.4%	807.7 / 67.5	227	G86G (GGC→GGG)	uacT	uric acid permease
Reads supporting (aligned to +/- strand): ref base C (7/15); new base G (76/128); total (83/144)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

CGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTA  >  CP009273/3024836‑3025130

cGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCg                                                                                                                                                   <  1:3150738/150‑1 (MQ=255)

cGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATAGGTATAGGGCg                                                                                                                                                   >  2:623464/1‑150 (MQ=255)

  tCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATTATTTGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATTCTCATTATCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATTTATAGGTATAGGCCGCt                                                                                                                                                 >  1:3535171/1‑150 (MQ=255)

    cTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCttt                                                                                                                                               <  1:1884290/150‑1 (MQ=255)

      tGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTAt                                                                                                                                             <  1:398675/150‑1 (MQ=255)

        tCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATgg                                                                                                                                           <  2:2599591/150‑1 (MQ=255)

                      gCAGTCGCTGTTCCTCTTATGATGGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCg                                                                                                                             <  2:3437381/150‑1 (MQ=255)

                       cAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATAGGTATCGGGCGCTTTATGGGGATACGCCAGCCgg                                                                                                                            >  1:1012375/1‑150 (MQ=255)

                        aGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGt                                                                                                                           <  2:691039/150‑1 (MQ=255)

                         gTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTg                                                                                                                          <  2:1055037/150‑1 (MQ=255)

                           cGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGAt                                                                                                                        >  1:1158957/1‑150 (MQ=255)

                            gCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGAtt                                                                                                                       <  1:1336560/150‑1 (MQ=255)

                             cTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTa                                                                                                                      <  1:3358540/150‑1 (MQ=255)

                              tGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTAt                                                                                                                     <  2:3801495/150‑1 (MQ=255)

                              tGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTAt                                                                                                                     >  1:1423056/1‑150 (MQ=255)

                               gTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATg                                                                                                                    <  1:4035884/150‑1 (MQ=255)

                               gTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATg                                                                                                                    >  2:710837/1‑150 (MQ=255)

                                  ccTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCACTGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCg                                                                                                                 <  1:1400648/150‑1 (MQ=255)

                                  ccTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCg                                                                                                                 <  2:3229910/150‑1 (MQ=255)

                                   ctctTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCgg                                                                                                                <  1:163877/150‑1 (MQ=255)

                                      ttATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCACTCTATCGGTATCGGGCGCTTAATGGGGCTCCGCCAGCCGGAGATTATGTCGGTGa                                                                                                             >  2:3463674/1‑150 (MQ=255)

                                      ttATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGa                                                                                                             >  2:2697985/1‑150 (MQ=255)

                                        aTGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAcc                                                                                                           >  2:190244/1‑150 (MQ=255)

                                          gATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCtt                                                                                                         <  1:3457623/150‑1 (MQ=255)

                                          gATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCtt                                                                                                         >  1:1526709/1‑150 (MQ=255)

                                          gATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCtt                                                                                                         >  1:1526553/1‑150 (MQ=255)

                                           aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        <  1:4658391/150‑1 (MQ=255)

                                           aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        <  2:3250781/150‑1 (MQ=255)

                                           aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        >  2:1456334/1‑150 (MQ=255)

                                              ggTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgct                                                                                                     <  1:3784556/150‑1 (MQ=255)

                                               gTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgctg                                                                                                    <  2:4468880/150‑1 (MQ=255)

                                               gTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGATCTTtgctg                                                                                                    <  2:3721308/150‑1 (MQ=255)

                                               gTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgctg                                                                                                    <  1:4270355/150‑1 (MQ=255)

                                               gTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgctg                                                                                                    <  2:4748481/150‑1 (MQ=255)

                                                 gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                  >  1:1969221/1‑150 (MQ=255)

                                                  aCCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGctgctg                                                                                                 <  1:1676699/150‑1 (MQ=255)

                                                       cTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAaca                                                                                            >  2:1643281/1‑150 (MQ=255)

                                                        tGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAacac                                                                                           >  1:982326/1‑150 (MQ=255)

                                                        tGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAacac                                                                                           >  1:576056/1‑150 (MQ=255)

                                                         gggCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACAcc                                                                                          >  2:1961963/1‑150 (MQ=255)

                                                           gCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCaa                                                                                        >  1:2172045/1‑150 (MQ=255)

                                                                 cAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAg                                                                                  <  1:3601616/150‑1 (MQ=255)

                                                                   aaaGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGcc                                                                                >  2:4428929/1‑150 (MQ=255)

                                                                    aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCa                                                                               >  1:4682511/1‑150 (MQ=255)

                                                                    aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCa                                                                               >  1:2302549/1‑150 (MQ=255)

                                                                      gaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAtt                                                                             <  2:2640721/150‑1 (MQ=255)

                                                                        agCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTgg                                                                           <  1:1775867/150‑1 (MQ=255)

                                                                         gCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTggg                                                                          >  1:2778996/1‑150 (MQ=255)

                                                                          cTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGa                                                                         <  1:4285608/150‑1 (MQ=255)

                                                                           tATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGAt                                                                        >  1:1796122/1‑150 (MQ=255)

                                                                           tATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGAt                                                                        <  2:1009542/150‑1 (MQ=255)

                                                                            aTTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATg                                                                       <  1:4350794/150‑1 (MQ=255)

                                                                                cGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAcc                                                                   <  2:2068778/150‑1 (MQ=255)

                                                                                cGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAcc                                                                   >  1:23305/1‑150 (MQ=255)

                                                                                cGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAcc                                                                   >  1:21425/1‑150 (MQ=255)

                                                                                 gATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  <  1:4214748/150‑1 (MQ=255)

                                                                                 gATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  <  1:1741069/150‑1 (MQ=255)

                                                                                 gATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  >  2:1493133/1‑150 (MQ=255)

                                                                                  aTGCTCATGAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTAGGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCg                                                                 <  2:1400457/150‑1 (MQ=255)

                                                                                       cATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGatat                                                            >  1:2571075/1‑150 (MQ=255)

                                                                                        aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           <  1:2024171/150‑1 (MQ=255)

                                                                                         ttAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCg                                                          <  1:1643726/150‑1 (MQ=255)

                                                                                             cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                      <  2:26498/150‑1 (MQ=255)

                                                                                             cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                      <  1:1024701/150‑1 (MQ=255)

                                                                                             cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                      <  1:1491127/150‑1 (MQ=255)

                                                                                             cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                      <  1:3880175/150‑1 (MQ=255)

                                                                                                ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                   <  2:94542/150‑1 (MQ=255)

                                                                                                ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                   <  1:4122066/150‑1 (MQ=255)

                                                                                                   tctcTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTggg                                                >  1:3600622/1‑150 (MQ=255)

                                                                                                   tctcTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTggg                                                >  1:1208853/1‑150 (MQ=255)

                                                                                                    ctctTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTgggg                                               <  2:83158/150‑1 (MQ=255)

                                                                                                       ttttGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGata                                            <  2:755427/150‑1 (MQ=255)

                                                                                                        tttGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGatat                                           <  1:2903015/150‑1 (MQ=255)

                                                                                                          tgctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATAttt                                         <  2:2045012/150‑1 (MQ=255)

                                                                                                             tgcGGGATCGTCACATTATTGCAATGGATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                      <  2:1248552/150‑1 (MQ=255)

                                                                                                              gcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTg                                     <  2:408276/150‑1 (MQ=255)

                                                                                                                  gATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCAc                                 >  2:2309064/1‑150 (MQ=255)

                                                                                                                   aTCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACt                                >  1:2947812/1‑150 (MQ=255)

                                                                                                                    tCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTa                               >  2:3825384/1‑150 (MQ=255)

                                                                                                                     cGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTAt                              <  2:3557468/150‑1 (MQ=255)

                                                                                                                     cGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTAt                              >  2:2558073/1‑150 (MQ=255)

                                                                                                                      gTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                             >  1:65914/1‑150 (MQ=255)

                                                                                                                      gTCACATTAGTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                             <  2:3353123/150‑1 (MQ=255)

                                                                                                                          caTTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgccg                         >  1:4515908/1‑150 (MQ=255)

                                                                                                                           attattGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgccgc                        >  2:3913080/1‑150 (MQ=255)

                                                                                                                              attGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCggg                     >  1:4056960/1‑150 (MQ=255)

                                                                                                                               ttGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGt                    <  1:4140731/150‑1 (MQ=255)

                                                                                                                                 gCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGttt                  <  2:2744463/150‑1 (MQ=255)

                                                                                                                                 gCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGttt                  <  2:4281682/150‑1 (MQ=255)

                                                                                                                                  cAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtttt                 <  2:2679058/150‑1 (MQ=255)

                                                                                                                                  cAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtttt                 <  2:4611474/150‑1 (MQ=255)

                                                                                                                                   aaTGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTa                <  1:1125285/150‑1 (MQ=255)

                                                                                                                                     tGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATc              >  1:2935965/1‑150 (MQ=255)

                                                                                                                                            gtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACat       >  2:4150098/1‑150 (MQ=255)

                                                                                                                                             tatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatt      >  1:1488707/1‑150 (MQ=255)

                                                                                                                                              atcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatta     <  2:4439188/150‑1 (MQ=255)

                                                                                                                                               tcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGTGATGAACCCGGATATCGTCCTGCTGGGGATATTTGGTGCAACTATCGCCGCGGGTTTTATCACCACattat    <  1:935152/150‑1 (MQ=255)

                                                                                                                                               tcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattat    <  2:305586/150‑1 (MQ=255)

                                                                                                                                               tcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattat    >  2:2099937/1‑150 (MQ=255)

                                                                                                                                                cggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattatt   <  2:2722726/150‑1 (MQ=255)

CGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTA  >  CP009273/3024836‑3025130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`