Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,755 T→G 23.0% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,7550TG23.0% 373.3 / 57.2 237F231V (TTC→GTC) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (56/122);  new base G (54/5);  total (110/127)
Fisher's exact test for biased strand distribution p-value = 7.69e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

TCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGT  >  CP009273/3080606‑3080904
                                                                                                                                                     |                                                                                                                                                       
tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt                                                                                                                                                         >  2:701818/1‑150 (MQ=255)
  gATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtgtc                                                                                                                                                       >  1:57264/1‑150 (MQ=255)
    tGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttcgt                                                                                                                                                     <  1:3410304/150‑1 (MQ=255)
       tGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAt                                                                                                                                                  >  1:3457936/1‑150 (MQ=255)
        gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc                                                                                                                                                 <  2:4024148/150‑1 (MQ=255)
           gagCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑t                                                                                                                                             <  2:1162361/148‑1 (MQ=255)
           gagCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑t                                                                                                                                             >  1:4199041/3‑150 (MQ=255)
            agCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGG‑T‑g                                                                                                                                           >  1:4468363/2‑150 (MQ=255)
               tttCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGG‑T‑GGcc                                                                                                                                        >  2:1204446/1‑150 (MQ=255)
                 tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCa                                                                                                                                      <  2:3015457/150‑1 (MQ=255)
                   cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAAt                                                                                                                                    <  2:4534340/150‑1 (MQ=255)
                   cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATAGG‑T‑GGCCCact                                                                                                                                    >  2:2155410/1‑148 (MQ=255)
                     cTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAGTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATgg                                                                                                                                  <  2:1281454/150‑1 (MQ=255)
                      tCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATggg                                                                                                                                 <  1:4608000/150‑1 (MQ=255)
                       cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGt                                                                                                                                <  2:878481/150‑1 (MQ=255)
                       cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGG‑TGGGCCCAATggg                                                                                                                                 >  1:3253853/1‑150 (MQ=255)
                         gCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATAGG‑T‑GGCCCACAGGGTgg                                                                                                                              >  1:3907383/1‑149 (MQ=255)
                          cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCAATGGGTGAc                                                                                                                             >  2:2571161/1‑150 (MQ=255)
                            ctctGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTg                                                                                                                           <  1:4355439/150‑1 (MQ=255)
                                 aaGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCCATGGGTGACGGCGGTc                                                                                                                      >  2:2416221/1‑150 (MQ=255)
                                  agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCt                                                                                                                     <  1:1068343/150‑1 (MQ=255)
                                     gaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGAc                                                                                                                  <  1:2108062/150‑1 (MQ=255)
                                     gaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGG‑T‑GGCCCAATGGGTGACTGCGGTCTGAc                                                                                                                  >  2:2893036/1‑150 (MQ=255)
                                     gaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGG‑T‑GGCCCAATGGGTGACGGCGGTCTGAc                                                                                                                  >  2:4257330/1‑150 (MQ=255)
                                      aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACt                                                                                                                 >  2:4007040/1‑150 (MQ=255)
                                      aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTTTCGG‑T‑GGGCCCATGGGGGACTGCGGTCTGGCt                                                                                                                 >  2:1617887/1‑150 (MQ=255)
                                        cGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTgg                                                                                                               >  1:1110666/1‑150 (MQ=255)
                                            cAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGt                                                                                                           <  1:2581470/150‑1 (MQ=255)
                                            cAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGt                                                                                                           <  1:4009784/150‑1 (MQ=255)
                                                aaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATAGG‑T‑GGCCCCAAGGGTGGCTGCGGGCTGACTGGGGGTaaaa                                                                                                       >  1:3673520/1‑150 (MQ=255)
                                                  tctcTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAtt                                                                                                     <  2:4358713/147‑1 (MQ=255)
                                                  tCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCAGACTGGTCGGAAAAtt                                                                                                     >  2:601998/1‑150 (MQ=255)
                                                   cGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGG‑T‑GGGCCACTGGGTGGCGGCGGTGTGACTGGTCGGAAAATTa                                                                                                    >  2:3261114/1‑150 (MQ=255)
                                                    gCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                   <  1:3926931/150‑1 (MQ=255)
                                                    gCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                   <  1:4283550/150‑1 (MQ=255)
                                                        cAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGtt                                                                                               >  2:3413134/1‑150 (MQ=255)
                                                         aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                              >  2:712062/1‑150 (MQ=255)
                                                         aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                              <  1:4757107/150‑1 (MQ=255)
                                                         aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                              <  1:2377034/150‑1 (MQ=255)
                                                         aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                              <  2:2652694/150‑1 (MQ=255)
                                                               cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCt                                                                                        <  2:536784/150‑1 (MQ=255)
                                                               cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGGTGATAACt                                                                                        >  2:4662038/1‑150 (MQ=255)
                                                                   aaTGGAAGTGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGCTACCTAcgg                                                                                    >  2:3143135/1‑150 (MQ=255)
                                                                   aaTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGG‑T‑GGCCCCAATGGTGGATGCGGTCTGACTGGTAGTAAAATTAAAGTTTATACCTAcgg                                                                                    >  1:2666765/1‑150 (MQ=255)
                                                                    aTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcggc                                                                                   >  2:3951143/1‑150 (MQ=255)
                                                                     tGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcggcg                                                                                  <  2:1992402/150‑1 (MQ=255)
                                                                        aaGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCa                                                                               >  2:1720551/1‑150 (MQ=255)
                                                                          gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATg                                                                             <  1:1747041/150‑1 (MQ=255)
                                                                          gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATg                                                                             <  1:3547802/150‑1 (MQ=255)
                                                                          gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTAGTTATAGG‑T‑GGCCCAAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGTTAACAAACGCGGCaag                                                                             >  1:1759221/1‑148 (MQ=255)
                                                                           agaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTTTAGGTT‑GGCCCAA‑GGGTGTCTGCGGTGGGTCTGGTGGTTAAATTATAGTTGTTAACAAAGGCGGCCTgg                                                                            >  1:1633832/1‑150 (MQ=255)
                                                                            gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGgc                                                                           <  1:4308342/150‑1 (MQ=255)
                                                                            gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACGGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCAAAGGCGGCATGgc                                                                           >  2:2922534/1‑150 (MQ=255)
                                                                            gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAAAGGGTGACTGCGGTCTGACTGGGCGTAAAATTAACGTTGATACCTACGGCGGCATGgc                                                                           >  2:1138433/1‑150 (MQ=255)
                                                                             atcatcAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCAACGGCGGCATGgcg                                                                          >  2:2590006/1‑150 (MQ=255)
                                                                              tcatcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGGGGGTTTAGG‑TGGGCCCAAT‑GGTGGCGGCGGTCTGGCGGGTGGGGAAATTATAGGTGTTAACAAACGCGGCAAGgcgc                                                                         >  1:3985244/1‑150 (MQ=255)
                                                                                 tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTc                                                                      <  1:621435/150‑1 (MQ=255)
                                                                                  caAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCa                                                                     <  2:1878727/150‑1 (MQ=255)
                                                                                   aaGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCAc                                                                    >  2:1956890/1‑150 (MQ=255)
                                                                                       cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATAGG‑T‑GGGCCCATGGGGGACTGCGGTCTGACTGGTCGTAAAAATATAGTTTTTTACAAACGCGGCATGGCGCGGCACCGTg                                                                >  1:4210411/1‑150 (MQ=255)
                                                                                       cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTCCGGCGGCATGGCGCGTCACGGTg                                                                <  2:1880678/150‑1 (MQ=255)
                                                                                         aTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCCATGGGTGACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATACCTAAGGCGGCCTGGCGCGTCACGGTGGc                                                              >  2:3307353/1‑150 (MQ=255)
                                                                                           tCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCgg                                                            <  1:74485/150‑1 (MQ=255)
                                                                                           tCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTGTAGG‑T‑GGCCCCAAGGGGGACTGCGGGCTGTCTGGTCGGGAAATTATCGTTGATTACTAACGCGGCCAGGCGCGGCCCGGGGGCgg                                                            >  1:620116/1‑150 (MQ=255)
                                                                                            cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGt                                                           <  1:2236804/150‑1 (MQ=255)
                                                                                             tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCAAAGGGGGTCTGCGGTGTGACTGGTCGTTAAATTATAGGTTATACCAACGGCGGCAAGGCGCGTCACCCTGGCgggg                                                          >  2:3574063/1‑148 (MQ=255)
                                                                                               cccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCCAAGGGTGTCTGCGGTCTGTCTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCa                                                        >  1:1961547/1‑150 (MQ=255)
                                                                                                  gCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtc                                                     <  1:1198291/150‑1 (MQ=255)
                                                                                                   cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtct                                                    <  1:3073963/150‑1 (MQ=255)
                                                                                                   cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtct                                                    <  1:2220891/150‑1 (MQ=255)
                                                                                                    tGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGG‑T‑GGGCCCATTGGTGGAAGCGGTCTGTCTGGTCGTAAAATTATATTTTATACATACCGCGGCAATGCGCGTCACGGGGGCGGTGCATtatc                                                   >  1:2928841/1‑150 (MQ=255)
                                                                                                    tGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTAGTTATAGG‑TGGGCCCCAT‑GGTGTCTGCGGTCTGCCTGGTCGTAAAAATATAGTTTATAACAACGGCGGCAAGGCGCGTCACGGTGGCGGTGCATtctc                                                   >  2:1577329/1‑150 (MQ=255)
                                                                                                    tGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGG‑T‑GGGCCCAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGTTACCTAAGGCGGCATGGCGCGTCACCGTGGCGGTGCAttata                                                   >  1:3764096/1‑147 (MQ=255)
                                                                                                         ggCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATCGG‑T‑GGCCCCAAGGGTGACAGCGGGCTGACAGGTCGTAAAAATAAAGTTGATAACAACCGCGGCCTGGCGCGGCACCGGGGCGGGGCCTACTCTGGTa                                              >  1:2986919/1‑150 (MQ=255)
                                                                                                          gCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaa                                             <  1:4251686/150‑1 (MQ=255)
                                                                                                           cTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGG‑T‑GGCCCAATTGGTGTCTGCGGTCTGTCTGGTCGTTAAATTATCGTTTATACCAACGGCGGCATTGCGCGTCACGGTGGCGGTGGATTATCTGGTaaa                                            >  2:3419415/1‑150 (MQ=255)
                                                                                                               cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGG‑T‑GGCCCCAAGGGTGACTGCGGTCTTACAGGTCGTAAAAATATAATTGATAACAAACGCGGCATGGCGCGTCACCGGGGCGGTGCCTACTATGGTAAatata                                        >  1:2980634/1‑149 (MQ=255)
                                                                                                                ttCTGCCAACAAATTCTTCATAAACCCGACCGGTCGTGTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTAGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATcc                                       <  1:3214319/150‑1 (MQ=255)
                                                                                                                   tGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGG‑T‑GGCCCAAATGGTGTCTGCGGTCTGACTGGTCGTAAAAATATAGTTGATAACAACGGCGGCATTGCGCGTCACCGTGGCGGTGCATTCTATGGTAAatataaaaa                                    >  1:3115893/1‑145 (MQ=255)
                                                                                                                    gCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCa                                   <  2:2327622/150‑1 (MQ=255)
                                                                                                                    gCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCa                                   <  1:913322/150‑1 (MQ=255)
                                                                                                                    gCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGG‑T‑GGCCCAAATGGTTAAGGCGGTCTGACTGGTAGTAAAATTATCTTTTTTAACTAACGCGGCAAGGCGCGGCACGGTGGCGGTGCATTATCTGGTAAAGATcaaaaa                                   >  2:4138965/1‑145 (MQ=255)
                                                                                                                      caccaAATTCTTCATCAACCCGACCGGTCGTGTCGTTAAAGG‑T‑GGCCCAAAGGGTGACTGCGGTCTGACTGGTCGTTAAAATAACGTTGATAACTACCGCGGCCTGGCGCCTCACCGGGGCGGTGGATTCTCAGGTAAAAATACATCaaa                                 >  2:1216558/1‑150 (MQ=255)
                                                                                                                       accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaaaa                                <  2:2830176/150‑1 (MQ=255)
                                                                                                                         caAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGt                              <  2:3555543/150‑1 (MQ=255)
                                                                                                                               cttcATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCg                        <  2:4425757/150‑1 (MQ=255)
                                                                                                                                  catcaACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTc                     <  1:3603524/150‑1 (MQ=255)
                                                                                                                                    tcaACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCg                   <  2:2128947/150‑1 (MQ=255)
                                                                                                                                    tcaACCCGACCGGTCGTGTCGTTATAGG‑T‑GGCCCAAAGGGGGTCTGCGGTCTGACTGGTCGGAAAATTATCGTTGATAACAACGGCGGCATGGCGCGTCACGGTGGCGGGGGATTCGATGGTAAAAATAAATCAAAAGTGGACCCGTCCg                   >  2:428801/1‑150 (MQ=255)
                                                                                                                                      aaCCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCa                 <  1:2139577/150‑1 (MQ=255)
                                                                                                                                         ccGACCGGTCGTGTCGGTATAGG‑T‑GGCCCCAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTTATACCTACGGCGGCCAGGCGCGTCACGGTGGCGGTGCAATCACTGGTAAACATACCAAAAAAGTGGACCCTTCCCCAGcc              >  2:1067777/1‑150 (MQ=255)
                                                                                                                                          cGACCGGTCGTTTCGTGATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCt             <  1:2894273/150‑1 (MQ=255)
                                                                                                                                           gACCGGTCGTGTCGTTATCGG‑T‑TGCCCAATGGGTGACTGCGGTGTGACTGGTCGTAAAAATAAAGTTGATAACAAAGGCGGCAAGGCGCGTCACCGTGGCGGGGCAATCTCATGTAAATATACATAAAAAGTGGAACGGTACGCAGccaa            >  2:1535912/1‑148 (MQ=255)
                                                                                                                                             ccGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACg          <  1:1617868/150‑1 (MQ=255)
                                                                                                                                             ccGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACg          <  1:1175453/150‑1 (MQ=255)
                                                                                                                                               ggTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgca        <  2:3812432/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgcag       >  2:3650529/1‑150 (MQ=255)
                                                                                                                                                    tttcgttATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACg   >  2:1301773/1‑150 (MQ=255)
                                                                                                                                                    tttcgttATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACg   <  1:1518454/150‑1 (MQ=255)
                                                                                                                                                     ttcgttATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGt  >  1:101444/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGG‑T‑GGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGT  >  CP009273/3080606‑3080904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑