Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,111,434 C→A 91.6% A346A (GCG→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,111,4340CA91.6% 850.8 / 63.7 275A346A (GCG→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (12/11);  new base A (144/108);  total (156/119)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 275 total aligned reads displayed.

CAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACG  >  CP009273/3111287‑3111580
                                                                                                                                                   |                                                                                                                                                  
      cGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGACCAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGACTAAGCTGATCAATATTCGCCCcgcg                                                                                                                                            >  2:3872766/1‑150 (MQ=255)
       gCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGa                                                                                                                                           >  2:4512114/1‑150 (MQ=255)
       gCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGa                                                                                                                                           >  2:2744527/1‑150 (MQ=255)
        cACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGaa                                                                                                                                          >  1:2494964/1‑150 (MQ=255)
               ggATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtcatc                                                                                                                                   >  1:2434774/1‑150 (MQ=255)
                 aTAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAc                                                                                                                                 <  1:3399220/150‑1 (MQ=255)
                  tAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAcc                                                                                                                                >  2:4190285/1‑150 (MQ=255)
                   aaCGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAccc                                                                                                                               >  2:1434946/1‑150 (MQ=255)
                   aaCGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAccc                                                                                                                               >  1:4288378/1‑150 (MQ=255)
                   aaCGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAccc                                                                                                                               >  2:3146732/1‑150 (MQ=255)
                     cGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAg                                                                                                                             >  2:574639/1‑150 (MQ=255)
                     cGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAg                                                                                                                             >  2:4054139/1‑150 (MQ=255)
                       agagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGtt                                                                                                                           <  2:2458963/150‑1 (MQ=255)
                        gagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtc                                                                                                                          >  1:1252285/1‑150 (MQ=255)
                                 aaTCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAgcgc                                                                                                                 >  1:1983385/1‑150 (MQ=255)
                                    ctcGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAAt                                                                                                              >  2:1128181/1‑150 (MQ=255)
                                    ctcGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAAt                                                                                                              <  2:3465097/150‑1 (MQ=255)
                                          gATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGa                                                                                                        >  2:1093249/1‑150 (MQ=255)
                                           aTCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGAc                                                                                                       >  1:1601249/1‑150 (MQ=255)
                                            tCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACt                                                                                                      >  2:3686199/1‑150 (MQ=255)
                                            tCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACt                                                                                                      >  1:3845340/1‑150 (MQ=255)
                                              aCGTTGGGATATTCGGCAAAGACCGTGCGTACATCGTCCGGAACCACACGGGTATTCTTTGGCCCGGTCGTCGAAGAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTa                                                                                                    <  2:2884391/150‑1 (MQ=255)
                                               cGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAt                                                                                                   <  2:3828388/150‑1 (MQ=255)
                                                gTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAtt                                                                                                  >  1:2812508/1‑150 (MQ=255)
                                                gTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAtt                                                                                                  >  2:3423252/1‑150 (MQ=255)
                                                  tGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGc                                                                                                <  1:839282/150‑1 (MQ=255)
                                                  tGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGc                                                                                                >  1:3823757/1‑150 (MQ=255)
                                                   gggATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTCTTATTTTGCCCGGTCGTCGAATAGCGCTGAATAAGCTGATCCAGATTAGCCCCGCGACCTTCATCCCCCAGGTCAGTCAGCGCACTGGTCGACTTCTTGcc                                                                                               >  1:1825643/1‑150 (MQ=255)
                                                    ggATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcg                                                                                              <  2:2228819/150‑1 (MQ=255)
                                                     gATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcgc                                                                                             <  1:3902558/150‑1 (MQ=255)
                                                     gATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcgc                                                                                             >  2:4286265/1‑150 (MQ=255)
                                                        atTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCa                                                                                          <  1:4165134/150‑1 (MQ=255)
                                                        atTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCa                                                                                          >  1:1063909/1‑150 (MQ=255)
                                                          tCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACt                                                                                        >  2:1957113/1‑150 (MQ=255)
                                                           cGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTg                                                                                       >  1:2929935/1‑150 (MQ=255)
                                                            ggCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGTCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                      <  2:1320146/150‑1 (MQ=255)
                                                            ggCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                      >  1:512912/1‑150 (MQ=255)
                                                            ggCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                      <  2:4669885/150‑1 (MQ=255)
                                                              cAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAc                                                                                    >  1:4656440/1‑150 (MQ=255)
                                                              cAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGCTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAc                                                                                    >  1:874455/1‑150 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                   >  2:1533600/1‑150 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                   <  2:2691381/150‑1 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                   >  1:801315/1‑150 (MQ=255)
                                                                aaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAccc                                                                                  >  1:4095441/1‑150 (MQ=255)
                                                                aaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAccc                                                                                  >  1:998239/1‑150 (MQ=255)
                                                                 aGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCa                                                                                 <  2:1792981/150‑1 (MQ=255)
                                                                  gACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAg                                                                                <  1:921164/150‑1 (MQ=255)
                                                                   aCCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGt                                                                               >  2:1480641/1‑150 (MQ=255)
                                                                        gCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCaaa                                                                          >  1:4515953/1‑150 (MQ=255)
                                                                         cGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAg                                                                         <  2:80656/150‑1 (MQ=255)
                                                                           tACATCGTCCGGAACAACACGGGTATTATTGTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGt                                                                       <  2:3541860/150‑1 (MQ=255)
                                                                            aCATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTa                                                                      <  2:287232/150‑1 (MQ=255)
                                                                            aCATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTa                                                                      >  2:508880/1‑150 (MQ=255)
                                                                             cATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                     <  2:3297644/150‑1 (MQ=255)
                                                                                cGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGa                                                                  >  1:2261470/1‑150 (MQ=255)
                                                                                 gTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGAt                                                                 <  1:2764107/150‑1 (MQ=255)
                                                                                  tCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATa                                                                <  2:4437564/150‑1 (MQ=255)
                                                                                     ggAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCa                                                             >  1:2636228/1‑150 (MQ=255)
                                                                                     ggAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCa                                                             <  1:3146445/150‑1 (MQ=255)
                                                                                      gAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaa                                                            >  1:1952229/1‑150 (MQ=255)
                                                                                         caacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAgg                                                         <  1:3074543/150‑1 (MQ=255)
                                                                                            cacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGgaga                                                      >  1:2864432/1‑150 (MQ=255)
                                                                                              cGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAta                                                    <  1:3002744/150‑2 (MQ=255)
                                                                                                 gTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGtt                                                 <  1:1275754/150‑1 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAATGAGATGGTTTc                                               <  1:4254757/150‑1 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  2:4379152/150‑1 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  1:1872662/150‑1 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  1:470002/150‑1 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                               <  1:544734/150‑1 (MQ=255)
                                                                                                     tattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGc                                             >  2:3740178/1‑150 (MQ=255)
                                                                                                     tattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGc                                             >  2:179587/1‑150 (MQ=255)
                                                                                                      attTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGcc                                            >  1:1426117/1‑150 (MQ=255)
                                                                                                      attTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGcc                                            >  2:821229/1‑150 (MQ=255)
                                                                                                      attTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGcc                                            >  1:3816758/1‑150 (MQ=255)
                                                                                                         ttGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCa                                         <  1:671339/150‑1 (MQ=255)
                                                                                                            cccGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCt                                      >  2:3028029/1‑150 (MQ=255)
                                                                                                                 tcgtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAgg                                 <  2:3945353/150‑1 (MQ=255)
                                                                                                                 tcgtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAgg                                 <  2:872917/150‑1 (MQ=255)
                                                                                                                    tcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaa                              <  2:4685209/150‑1 (MQ=255)
                                                                                                                     cgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaaa                             <  1:1338847/150‑1 (MQ=255)
                                                                                                                     cgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaaa                             >  1:206285/1‑150 (MQ=255)
                                                                                                                     cgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaaa                             >  2:2544388/1‑150 (MQ=255)
                                                                                                                          aGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAc                        >  2:2124633/1‑150 (MQ=255)
                                                                                                                          aGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAc                        >  1:1655417/1‑150 (MQ=255)
                                                                                                                             gATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAt                     <  1:3520643/150‑1 (MQ=255)
                                                                                                                                 aaTAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTc                 <  2:1736727/150‑1 (MQ=255)
                                                                                                                                  aTAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcc                >  1:1160469/1‑150 (MQ=255)
                                                                                                                                    aaGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc              >  1:3770961/1‑150 (MQ=255)
                                                                                                                                    aaGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc              >  1:2189216/1‑150 (MQ=255)
                                                                                                                                    aaGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc              <  1:1477553/150‑1 (MQ=255)
                                                                                                                                    aaGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATAACAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc              >  1:473706/1‑150 (MQ=255)
                                                                                                                                     aGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCt             >  2:3766639/1‑150 (MQ=255)
                                                                                                                                        tGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTc          >  2:4059161/1‑150 (MQ=255)
                                                                                                                                         gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCa         <  2:1904822/150‑1 (MQ=255)
                                                                                                                                         gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCa         <  2:856766/150‑1 (MQ=255)
                                                                                                                                          aTCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCAc        >  1:4353828/1‑150 (MQ=255)
                                                                                                                                             aaTATTCGACCCGCGAAATTCATCACACAGTTCAGTCAGCGAAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc     <  1:1064973/150‑1 (MQ=255)
                                                                                                                                             aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc     >  1:3565408/1‑150 (MQ=255)
                                                                                                                                               tatTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAc   >  1:3618866/1‑150 (MQ=255)
                                                                                                                                                atTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACg  <  2:4607751/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACG  >  CP009273/3111287‑3111580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑