BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,173,669	G→T	90.4%	A189A (GCG→GCT)	ygiB →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,173,669	0	G	T	90.4%	1175.7 / 108.3	388	A189A (GCG→GCT)	ygiB	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base G (17/20); new base T (164/187); total (181/207)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 388 total aligned reads displayed.

ATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGG  >  CP009273/3173522‑3173815

 tttGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGacc                                                                                                                                                 <  2:3709884/150‑1 (MQ=255)

  ttGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTCTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTacca                                                                                                                                                <  1:1001073/150‑1 (MQ=255)

   tGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTaccac                                                                                                                                               >  2:785580/1‑150 (MQ=255)

     caccGCAGCCGCTGGTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTCTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTAccacca                                                                                                                                             <  2:2536099/150‑1 (MQ=255)

     cacaGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTAccacca                                                                                                                                             <  2:162188/150‑1 (MQ=255)

      acaGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACcaccac                                                                                                                                            <  1:757530/150‑1 (MQ=255)

      acaGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACcaccac                                                                                                                                            <  1:2738821/150‑1 (MQ=255)

      acaGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACcaccac                                                                                                                                            <  1:2406638/150‑1 (MQ=255)

       cagcagCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACt                                                                                                                                           <  2:4710222/150‑1 (MQ=255)

          cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTAcc                                                                                                                                        >  2:3375724/1‑150 (MQ=255)

           agcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCg                                                                                                                                       >  1:4457791/1‑150 (MQ=255)

              cGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTa                                                                                                                                    <  1:1447605/150‑1 (MQ=255)

               gCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAc                                                                                                                                   <  1:3726955/150‑1 (MQ=255)

               gCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAc                                                                                                                                   <  1:2349830/150‑1 (MQ=255)

                cTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAcc                                                                                                                                  <  1:1009629/150‑1 (MQ=255)

                cTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAcc                                                                                                                                  <  2:522660/150‑1 (MQ=255)

                  gTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCg                                                                                                                                >  2:2629890/1‑150 (MQ=255)

                     ctcctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTgg                                                                                                                             >  2:425544/1‑150 (MQ=255)

                      tcctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGtt                                                                                                                                     <  1:4151714/141‑1 (MQ=255)

                       cctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCg                                                                                                                           >  1:798014/1‑150 (MQ=255)

                        ctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCgg                                                                                                                          >  1:2028067/1‑150 (MQ=255)

                           gAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGttt                                                                                                                       <  2:884082/150‑1 (MQ=255)

                            aaaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGtttt                                                                                                                      <  1:2157871/150‑1 (MQ=255)

                             aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTGTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTg                                                                                                                     <  2:102199/150‑1 (MQ=255)

                              aaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTgg                                                                                                                    >  1:3316202/1‑150 (MQ=255)

                              aaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTgg                                                                                                                    <  1:3985226/150‑1 (MQ=255)

                               aaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGt                                                                                                                   <  1:4261187/150‑1 (MQ=255)

                                aCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTg                                                                                                                  <  1:3388239/150‑1 (MQ=255)

                                aCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTg                                                                                                                  <  2:4383749/150‑1 (MQ=255)

                                aCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTg                                                                                                                  >  1:4510130/1‑150 (MQ=255)

                                 cccAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGa                                                                                                                 <  2:1138096/150‑1 (MQ=255)

                                    agccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATc                                                                                                              <  1:1746944/150‑1 (MQ=255)

                                      ccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                                                                            >  2:1457596/1‑150 (MQ=255)

                                       cagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGt                                                                                                           >  1:1928972/1‑150 (MQ=255)

                                       cagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGt                                                                                                           >  1:1355725/1‑150 (MQ=255)

                                        agTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGtt                                                                                                          <  1:1053492/150‑1 (MQ=255)

                                         gTCCGGCTTACGGTAACTCGCCCGACGCGACGGGTAAACACGAGGGCGCAGCCCAGCCAGGCCGCACCATGACCGTCCCGACGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTg                                                                                                         <  1:550853/150‑1 (MQ=255)

                                         gTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTg                                                                                                         >  2:2359503/1‑150 (MQ=255)

                                          tCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGc                                                                                                        >  2:4137365/1‑150 (MQ=255)

                                           ccGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGcc                                                                                                       >  1:887110/1‑150 (MQ=255)

                                             ggCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaa                                                                                                     >  1:2990260/1‑150 (MQ=255)

                                             ggCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaa                                                                                                     >  2:3157783/1‑150 (MQ=255)

                                                 tACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaacaa                                                                                                 >  1:3548264/1‑150 (MQ=255)

                                                   cGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAg                                                                                               <  1:3687796/150‑1 (MQ=255)

                                                    ggTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                                                                              >  2:3326749/1‑150 (MQ=255)

                                                     gTAAATATACCGACGCTACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                                                                             >  1:83385/1‑150 (MQ=255)

                                                     gTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                                                                             <  1:2203341/150‑1 (MQ=255)

                                                       aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACt                                                                                           <  1:3430065/150‑1 (MQ=255)

                                                       aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACt                                                                                           <  2:4121378/150‑1 (MQ=255)

                                                       aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACt                                                                                           >  1:2632908/1‑150 (MQ=255)

                                                        aaTAGACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTa                                                                                          <  1:4036056/150‑1 (MQ=255)

                                                          tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                        >  2:106573/1‑150 (MQ=255)

                                                          tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACAAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                        >  2:550576/1‑150 (MQ=255)

                                                               cgacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCg                                                                                   <  2:3444982/150‑1 (MQ=255)

                                                                gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGt                                                                                  <  2:38942/150‑1 (MQ=255)

                                                                gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGt                                                                                  >  2:2448915/1‑150 (MQ=255)

                                                                gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGt                                                                                  >  1:345869/1‑150 (MQ=255)

                                                                  cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAg                                                                                >  2:2956777/1‑150 (MQ=255)

                                                                    cgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTg                                                                              <  2:1238052/150‑1 (MQ=255)

                                                                      acgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCa                                                                            <  2:3286090/150‑1 (MQ=255)

                                                                        gggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAc                                                                          >  2:3219776/1‑150 (MQ=255)

                                                                             aaaaCTAGGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTa                                                                     <  2:3954989/150‑1 (MQ=255)

                                                                              aaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACAGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTAAGCAGCGTAGTGCAACCGGTAc                                                                    >  1:110375/1‑150 (MQ=255)

                                                                                   aTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtctt                                                               <  2:2018558/150‑1 (MQ=255)

                                                                                    tGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtcttc                                                              >  1:3224953/1‑150 (MQ=255)

                                                                                    tGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAACGCACTATGCAGCGTAGTGCACCCGGTCCCtcttc                                                              >  1:2496930/1‑150 (MQ=255)

                                                                                      gcgcAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTtctc                                                            >  2:3024709/1‑150 (MQ=255)

                                                                                          agcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                        <  2:969025/150‑1 (MQ=255)

                                                                                          agcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                        <  2:2048327/150‑1 (MQ=255)

                                                                                           gcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCa                                                       >  2:3497898/1‑150 (MQ=255)

                                                                                            cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCaa                                                      <  2:4548548/150‑1 (MQ=255)

                                                                                            cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCaa                                                      <  2:3595297/150‑1 (MQ=255)

                                                                                            cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCaa                                                      >  1:3190806/1‑150 (MQ=255)

                                                                                             ccagccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAAt                                                     <  1:589937/150‑1 (MQ=255)

                                                                                               agccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATgg                                                   >  2:618617/1‑150 (MQ=255)

                                                                                                 ccagGCCGCACCAGGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACGACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGGAGTGCAACCGGTACCTCTTCTCGTTCACTGGGt                                                 <  1:1221641/150‑1 (MQ=255)

                                                                                                    ggCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGc                                              <  2:4193837/150‑1 (MQ=255)

                                                                                                     gCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGTGTGGCt                                             >  1:2779690/1‑150 (MQ=255)

                                                                                                     gCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCt                                             >  1:4618003/1‑150 (MQ=255)

                                                                                                       cGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                           >  2:7492/1‑150 (MQ=255)

                                                                                                       cGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                           >  2:4068366/1‑150 (MQ=255)

                                                                                                       cGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                           >  2:9074/1‑150 (MQ=255)

                                                                                                       cGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                           >  1:1589547/1‑150 (MQ=255)

                                                                                                       cGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                           >  2:1718713/1‑150 (MQ=255)

                                                                                                          aCCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                        >  2:2582918/1‑150 (MQ=255)

                                                                                                          aCCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                        <  1:4381882/150‑1 (MQ=255)

                                                                                                          aCCATGACCGTAACGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                        <  1:1402455/150‑1 (MQ=255)

                                                                                                           ccATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAcc                                       <  1:2956777/150‑1 (MQ=255)

                                                                                                               gACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATg                                   >  2:4276517/1‑150 (MQ=255)

                                                                                                               gACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATg                                   >  1:4743024/1‑150 (MQ=255)

                                                                                                                    tACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAag                              <  1:6583/150‑1 (MQ=255)

                                                                                                                          aGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAg                        <  2:4461903/150‑1 (MQ=255)

                                                                                                                            aCGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTa                      <  2:2714161/150‑1 (MQ=255)

                                                                                                                                 aaTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAcc                 <  1:785511/150‑1 (MQ=255)

                                                                                                                                   tGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGa               >  2:1304883/1‑150 (MQ=255)

                                                                                                                                    ggCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAg              >  1:1537560/1‑150 (MQ=255)

                                                                                                                                     gCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAgc             <  2:65801/150‑1 (MQ=255)

                                                                                                                                       aCCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAgcgc           <  2:4585842/150‑1 (MQ=255)

                                                                                                                                                ggCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTgg  >  1:3824147/1‑150 (MQ=255)

                                                                                                                                                ggCTACCACCACTACCGTTACCCGTGGCGGTTGTGGTGAATCTGTTGCCAAACACAGCACGATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCACTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTgg  <  2:3018018/150‑1 (MQ=255)

ATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGG  >  CP009273/3173522‑3173815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`