Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,245,848 G→T 84.8% L62L (CTG→CTT)  yhaH → DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,245,8480GT84.8% 597.3 / 116.7 245L62L (CTG→CTT) yhaHDUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (22/15);  new base T (103/105);  total (125/120)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

Only 100 of 247 total aligned reads displayed.

GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245699‑3245993
                                                                                                                                                     |                                                                                                                                                 
ggTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTg                                                                                                                                                   >  1:2399290/1‑150 (MQ=255)
  tttCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGACGGCATCCTGACGACAATCTACGGTATTCTTgt                                                                                                                                                 >  1:4670491/1‑150 (MQ=255)
   ttCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTtgtg                                                                                                                                                >  2:192460/1‑150 (MQ=255)
     ccGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtt                                                                                                                                              <  2:1713676/150‑1 (MQ=255)
      cGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttt                                                                                                                                             <  1:4253681/150‑1 (MQ=255)
        gggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttttt                                                                                                                                           <  2:2357473/150‑1 (MQ=255)
         ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTa                                                                                                                                          <  1:2909052/150‑1 (MQ=255)
          gTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTAc                                                                                                                                         <  2:3652217/150‑1 (MQ=255)
           tCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAcc                                                                                                                                        >  1:3802860/1‑150 (MQ=255)
            cGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCg                                                                                                                                       <  2:3593742/150‑1 (MQ=255)
              tGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtg                                                                                                                                     <  2:2574651/150‑1 (MQ=255)
                  cggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGtggtgg                                                                                                                                 <  2:2978715/150‑1 (MQ=255)
                       cAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt                                                                                                                            >  2:2919486/1‑150 (MQ=255)
                        aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  2:2443386/1‑150 (MQ=255)
                        aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTACTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  2:4097868/1‑150 (MQ=255)
                         aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTc                                                                                                                          >  1:1467744/1‑150 (MQ=255)
                          agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                                         >  2:4374541/1‑150 (MQ=255)
                          agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                                         <  1:30544/150‑1 (MQ=255)
                            agTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                                                       >  1:199151/1‑150 (MQ=255)
                            agTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                                                       >  2:1029447/1‑150 (MQ=255)
                             gTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGtt                                                                                                                      <  1:252837/150‑1 (MQ=255)
                             gTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGtt                                                                                                                      <  2:2302387/150‑1 (MQ=255)
                                  ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgcc                                                                                                                 <  1:4346639/150‑1 (MQ=255)
                                   gATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgccg                                                                                                                >  1:1351778/1‑150 (MQ=255)
                                   gATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgccg                                                                                                                >  1:3413525/1‑150 (MQ=255)
                                     tGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCgccgcc                                                                                                              <  2:211224/150‑1 (MQ=255)
                                      gTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCt                                                                                                             >  1:3697075/1‑150 (MQ=255)
                                        ttATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGc                                                                                                           <  2:1953835/150‑1 (MQ=255)
                                              tGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGaca                                                                                                     >  2:166060/1‑150 (MQ=255)
                                               ggTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGacac                                                                                                    >  1:2886451/1‑150 (MQ=255)
                                                gTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACacc                                                                                                   >  2:2166876/1‑150 (MQ=255)
                                                           cTTTTCGTTCGTGCTGGGGCTGCTTTATAAAATGTTTGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTGTGGTGTTTTTACGGTGGTGGGCGTTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGc                                                                                        <  1:3677487/150‑1 (MQ=255)
                                                                cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                   >  1:2763398/1‑150 (MQ=255)
                                                                 gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  <  1:1004331/150‑1 (MQ=255)
                                                                 gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:3182691/1‑150 (MQ=255)
                                                                 gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:3180418/1‑150 (MQ=255)
                                                                    cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                                               >  2:4068338/1‑150 (MQ=255)
                                                                     gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  1:2247962/1‑150 (MQ=255)
                                                                     gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  1:4627586/1‑150 (MQ=255)
                                                                     gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  2:2264832/1‑150 (MQ=255)
                                                                            ggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                                       >  2:2024255/1‑150 (MQ=255)
                                                                                 ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                  <  2:4552967/150‑1 (MQ=255)
                                                                                 ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                  <  1:2668455/150‑1 (MQ=255)
                                                                                   ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAt                                                                >  1:682176/1‑150 (MQ=255)
                                                                                     aTAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATcc                                                              >  2:2332625/1‑150 (MQ=255)
                                                                                      tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATccc                                                             >  1:4186205/1‑150 (MQ=255)
                                                                                       aataTGTTAGGGGGGCATCGCGGTGGTGGCGATGGCATCGTGCCGACTATCGACGTTTTTCTTGTGTTTTTACCGTGGGGGGGGTTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  1:1592563/147‑1 (MQ=255)
                                                                                       aaatTGTTTGGCTGGCAAGGCGCTGGTGGCTAAGGCATCCTGACGACAATCTTCGGTATTCTTGTGTTTTTACGGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  1:3980590/150‑1 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTTTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  1:3701601/150‑1 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            >  2:2852356/1‑150 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            >  2:477850/1‑150 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  2:11478/150‑1 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          >  1:2659294/1‑150 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          <  2:4167677/150‑1 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          <  2:4167406/150‑1 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          <  2:1782095/150‑1 (MQ=255)
                                                                                          aTGTTAGGCTGGCAACGCGCTGGTGGCTAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         <  2:1748430/150‑1 (MQ=255)
                                                                                          aTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         <  2:4656663/150‑1 (MQ=255)
                                                                                            gTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAt                                                       >  2:2088656/1‑150 (MQ=255)
                                                                                              tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                     <  1:3770857/150‑1 (MQ=255)
                                                                                              tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                     <  2:2684931/150‑1 (MQ=255)
                                                                                               aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCgg                                                    <  2:2525538/150‑1 (MQ=255)
                                                                                                   tGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctgg                                                <  2:2242851/150‑1 (MQ=255)
                                                                                                      cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCACGTTTATCGGCTGGCtt                                             >  1:1201082/1‑150 (MQ=255)
                                                                                                      cAACGCGCTGGGGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtt                                             <  2:903645/150‑1 (MQ=255)
                                                                                                        aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                                           <  2:2399290/150‑1 (MQ=255)
                                                                                                         cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                                          <  1:1038532/150‑1 (MQ=255)
                                                                                                         cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                                          <  2:1092346/150‑1 (MQ=255)
                                                                                                          gcgcTGGTGGCGAAGGCATCCTGACGACAATCTCCGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatta                                         <  1:1065606/150‑1 (MQ=255)
                                                                                                           cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                                        >  2:3987699/1‑150 (MQ=255)
                                                                                                           cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                                        >  2:3986941/1‑150 (MQ=255)
                                                                                                            gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatc                                       >  2:3338115/1‑150 (MQ=255)
                                                                                                              tggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcat                                     <  2:441428/150‑1 (MQ=255)
                                                                                                              tggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTGTATCGGCTGGCTTATTatcat                                     <  2:4044328/150‑1 (MQ=255)
                                                                                                               ggtggCGAAGGCATCCTGACTACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcatc                                    >  1:3746281/1‑150 (MQ=255)
                                                                                                                 tggCGTAGGCATCCTGACGACAATCTCCGGTATTCTTGTGTTTGTACGGGGGGGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGt                                  <  1:2813253/150‑1 (MQ=255)
                                                                                                                   gCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt                                <  1:2489451/150‑1 (MQ=255)
                                                                                                                     gAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCttt                              <  2:1351778/150‑1 (MQ=255)
                                                                                                                        ggCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAc                           <  1:4019072/150‑1 (MQ=255)
                                                                                                                         gCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt                          >  1:766192/1‑150 (MQ=255)
                                                                                                                          cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                         <  2:3986409/150‑1 (MQ=255)
                                                                                                                          cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                         >  1:4449830/1‑150 (MQ=255)
                                                                                                                           aTCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGc                        >  2:4161111/1‑150 (MQ=255)
                                                                                                                             ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      <  1:1765071/150‑1 (MQ=255)
                                                                                                                             ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      >  1:1856013/1‑150 (MQ=255)
                                                                                                                              cTGACGACAATCTACGTTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                     <  1:192460/150‑1 (MQ=255)
                                                                                                                               tGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAgg                    >  1:1981894/1‑150 (MQ=255)
                                                                                                                                 acgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCg                  >  2:2979320/1‑150 (MQ=255)
                                                                                                                                  cgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCgg                 >  1:1168873/1‑150 (MQ=255)
                                                                                                                                   gacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCggg                >  2:100627/1‑150 (MQ=255)
                                                                                                                                     cAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTa              >  1:717043/1‑150 (MQ=255)
                                                                                                                                      aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             >  2:2666634/1‑150 (MQ=255)
                                                                                                                                      aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             <  2:1272797/150‑1 (MQ=255)
                                                                                                                                      aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             <  2:1272125/150‑1 (MQ=255)
                                                                                                                                       aTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACg            >  2:335742/1‑150 (MQ=255)
                                                                                                                                          tccGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCa         <  2:1072707/148‑1 (MQ=255)
                                                                                                                                              gTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCg     >  1:2537922/1‑150 (MQ=255)
                                                                                                                                              gTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACAGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCg     >  2:608547/1‑150 (MQ=255)
                                                                                                                                               tATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGa    >  1:4283031/1‑150 (MQ=255)
                                                                                                                                                aTTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGGTCCGCCGCCTGCTCGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGaa   <  2:4514426/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245699‑3245993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑