Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,375,260 G→T 100% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,375,2600GT96.3% 912.4 / 18.6 266D387Y (GAT→TAT) degQserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base T (122/134);  total (127/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAA  >  CP009273/3375113‑3375406
                                                                                                                                                   |                                                                                                                                                  
ggCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                                  <  1:3867393/150‑1 (MQ=255)
 gCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATg                                                                                                                                                 <  2:1730489/150‑1 (MQ=255)
 gCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTAGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATg                                                                                                                                                 <  1:4749228/150‑1 (MQ=255)
  cACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATgg                                                                                                                                                >  2:3528537/1‑150 (MQ=255)
   aCGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCCGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggc                                                                                                                                               >  2:1210344/1‑150 (MQ=255)
     gAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGACGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcgg                                                                                                                                             >  1:689773/1‑150 (MQ=255)
     gAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcgg                                                                                                                                             >  2:2047563/1‑150 (MQ=255)
     gAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcgg                                                                                                                                             >  2:848223/1‑150 (MQ=255)
      aaaGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcggc                                                                                                                                            >  2:1571962/1‑150 (MQ=255)
      aaaGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcggc                                                                                                                                            >  2:2395673/1‑150 (MQ=255)
      aaaGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcggc                                                                                                                                            <  1:4128826/150‑1 (MQ=255)
       aaGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCa                                                                                                                                           >  1:295748/1‑150 (MQ=255)
           gAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAgg                                                                                                                                       >  2:672602/1‑150 (MQ=255)
            aaGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGt                                                                                                                                      <  1:2135755/150‑1 (MQ=255)
              gCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAt                                                                                                                                    <  1:2494014/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                   >  1:4520051/1‑150 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                   <  2:1297940/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                   <  2:3658958/150‑1 (MQ=255)
                ttGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTa                                                                                                                                  <  2:883711/150‑1 (MQ=255)
                ttGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTa                                                                                                                                  <  2:2977875/150‑1 (MQ=255)
                 tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaa                                                                                                                                 <  2:3474536/150‑1 (MQ=255)
                 tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaa                                                                                                                                 >  1:3317715/1‑150 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGTGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                              >  1:1599050/1‑150 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                              >  2:2276931/1‑150 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                              <  1:670908/150‑1 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                              <  1:4600041/150‑1 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                              <  1:1847070/150‑1 (MQ=255)
                      tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCg                                                                                                                            <  2:1901616/150‑1 (MQ=255)
                        ctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGAt                                                                                                                          <  1:2765933/150‑1 (MQ=255)
                         tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATg                                                                                                                         <  1:1003141/150‑1 (MQ=255)
                             tAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGt                                                                                                                     <  2:4736677/150‑1 (MQ=255)
                              aaCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGtt                                                                                                                    >  2:4016133/1‑150 (MQ=255)
                               aCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTg                                                                                                                   >  1:2713753/1‑150 (MQ=255)
                                cGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGt                                                                                                                  >  2:3113319/1‑150 (MQ=255)
                                   cAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                               >  2:2507816/1‑150 (MQ=255)
                                    aaaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                              <  1:720500/150‑1 (MQ=255)
                                     aaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAg                                                                                                             >  2:585019/1‑150 (MQ=255)
                                        cACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGaa                                                                                                          >  2:789992/1‑150 (MQ=255)
                                        cACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGaa                                                                                                          <  2:2199429/150‑1 (MQ=255)
                                            ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGccc                                                                                                      >  2:3292116/1‑150 (MQ=255)
                                            ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGccc                                                                                                      >  1:1874102/1‑150 (MQ=255)
                                                     aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCa                                                                                             <  1:2428368/150‑1 (MQ=255)
                                                       tGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                           >  1:1102235/1‑150 (MQ=255)
                                                        gACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggc                                                                                          >  1:1418729/1‑150 (MQ=255)
                                                         aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggct                                                                                         >  1:3344908/1‑150 (MQ=255)
                                                         aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggct                                                                                         >  2:3599023/1‑150 (MQ=255)
                                                              cGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCtt                                                                                    <  1:1691730/150‑1 (MQ=255)
                                                               gATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTg                                                                                   >  1:714540/1‑150 (MQ=255)
                                                                aTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGc                                                                                  >  1:3753572/1‑150 (MQ=255)
                                                                aTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATAGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGc                                                                                  >  1:2482478/1‑150 (MQ=255)
                                                                     aGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaaa                                                                             >  2:2340658/1‑150 (MQ=255)
                                                                        aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                          >  2:1735243/1‑150 (MQ=255)
                                                                             ttCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtgt                                                                     <  2:4128273/150‑1 (MQ=255)
                                                                              tcgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtgtg                                                                    <  2:1404055/150‑1 (MQ=255)
                                                                                 tcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATc                                                                 >  2:2128115/1‑150 (MQ=255)
                                                                                  cgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCa                                                                <  1:18805/150‑1 (MQ=255)
                                                                                     ccAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                             <  2:3085183/150‑1 (MQ=255)
                                                                                     ccAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                             >  1:3646892/1‑150 (MQ=255)
                                                                                     ccAGCGCTGAAAGGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                             <  2:3443775/150‑1 (MQ=255)
                                                                                      cAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTgg                                                            >  2:3198270/1‑150 (MQ=255)
                                                                                         cgcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGt                                                         >  1:3783835/1‑150 (MQ=255)
                                                                                          gcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTc                                                        >  1:926846/1‑150 (MQ=255)
                                                                                          gcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTc                                                        <  1:3091682/150‑1 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                    <  2:396590/150‑1 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                    <  1:527260/150‑1 (MQ=255)
                                                                                                   aTCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAt                                               <  2:3157688/150‑1 (MQ=255)
                                                                                                    tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATc                                              >  1:1499643/1‑150 (MQ=255)
                                                                                                    tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATc                                              <  1:2154121/150‑1 (MQ=255)
                                                                                                      aCGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCgg                                            <  2:1878766/150‑1 (MQ=255)
                                                                                                       cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCggg                                           >  1:1171637/1‑150 (MQ=255)
                                                                                                       cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCggg                                           >  1:2055789/1‑150 (MQ=255)
                                                                                                           aGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGaa                                       <  1:3181245/150‑1 (MQ=255)
                                                                                                             cgcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt                                     >  1:4655982/1‑150 (MQ=255)
                                                                                                             cgcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt                                     >  1:1193785/1‑150 (MQ=255)
                                                                                                             cgcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt                                     <  2:3568354/150‑1 (MQ=255)
                                                                                                              gcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc                                    <  2:4269633/150‑1 (MQ=255)
                                                                                                              gcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc                                    <  1:4016133/150‑1 (MQ=255)
                                                                                                               cTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCg                                   >  2:3360520/1‑150 (MQ=255)
                                                                                                                tGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                                  <  1:1947240/150‑1 (MQ=255)
                                                                                                                tGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                                  <  1:36062/150‑1 (MQ=255)
                                                                                                                 ggAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAt                                 >  1:3196276/1‑150 (MQ=255)
                                                                                                                  gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAtt                                <  1:2371480/150‑1 (MQ=255)
                                                                                                                    aGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGc                              >  1:4458121/1‑150 (MQ=255)
                                                                                                                         cAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATAGCTGaaa                         >  1:3232141/1‑150 (MQ=255)
                                                                                                                             gTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCg                     <  2:449243/150‑1 (MQ=255)
                                                                                                                              ttGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGt                    >  1:1066946/1‑150 (MQ=255)
                                                                                                                               tGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTa                   <  2:3446689/150‑1 (MQ=255)
                                                                                                                               tGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTa                   >  2:1146167/1‑150 (MQ=255)
                                                                                                                                  gCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg                <  1:351998/150‑1 (MQ=255)
                                                                                                                                    gATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTg              <  2:4699993/150‑1 (MQ=255)
                                                                                                                                         tCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggc         <  1:3543759/150‑1 (MQ=255)
                                                                                                                                          cAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcg        <  2:3767260/150‑1 (MQ=255)
                                                                                                                                           aGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcgg       <  1:3408597/150‑1 (MQ=255)
                                                                                                                                           aGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcgg       >  1:2022877/1‑150 (MQ=255)
                                                                                                                                            gCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcggc      <  2:1603859/150‑1 (MQ=255)
                                                                                                                                            gCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcggc      >  1:825826/1‑150 (MQ=255)
                                                                                                                                              tAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa    >  2:4228349/1‑150 (MQ=255)
                                                                                                                                               aaaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaaa   >  2:4108649/1‑150 (MQ=255)
                                                                                                                                                aaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCACAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaaaa  <  2:2829027/150‑1 (MQ=255)
                                                                                                                                                aaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaaaa  <  2:1504875/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAA  >  CP009273/3375113‑3375406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑