BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,520,789	G→T	9.3%	R321L (CGA→CTA)	yrfF →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,520,789	0	G	T	9.3%	989.9 / 55.3	270	R321L (CGA→CTA)	yrfF	inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (135/109); new base T (14/12); total (149/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

CGAAAACGATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGA  >  CP009273/3520640‑3520938

cGAAAACGATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACg                                                                                                                                                       <  1:1036194/150‑1 (MQ=255)

 gAAAACGATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGa                                                                                                                                                      <  2:946837/150‑1 (MQ=255)

    aaCGATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGatat                                                                                                                                                   >  2:1694893/1‑150 (MQ=255)

    aaCGATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGatat                                                                                                                                                   <  1:1509098/150‑1 (MQ=255)

       gATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCtt                                                                                                                                                >  2:551821/1‑150 (MQ=255)

        aTCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCttt                                                                                                                                               <  2:1318289/150‑1 (MQ=255)

        aTCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCttt                                                                                                                                               <  1:511162/150‑1 (MQ=255)

        aTCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCttt                                                                                                                                               <  1:1393453/150‑1 (MQ=255)

         tCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTc                                                                                                                                              >  2:2266639/1‑150 (MQ=255)

          cAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCg                                                                                                                                             >  2:2182738/1‑150 (MQ=255)

          cAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCg                                                                                                                                             >  1:3930812/1‑150 (MQ=255)

              aaCAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGc                                                                                                                                         <  2:1098487/150‑1 (MQ=255)

                cAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCat                                                                                                                                       >  1:580074/1‑150 (MQ=255)

                 aGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCatg                                                                                                                                      >  1:4228763/1‑150 (MQ=255)

                 aGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCatg                                                                                                                                      <  1:3544000/150‑1 (MQ=255)

                   aTCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCatgat                                                                                                                                    <  1:2775808/150‑1 (MQ=255)

                    tCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCatgatg                                                                                                                                   <  2:4505618/150‑1 (MQ=255)

                       acaaTATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTGTCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAg                                                                                                                                <  2:200822/150‑1 (MQ=255)

                        caaTATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCTACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGt                                                                                                                               >  2:3765352/1‑150 (MQ=255)

                        caaTATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCTACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGt                                                                                                                               >  2:3445413/1‑150 (MQ=255)

                        caaTATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGt                                                                                                                               >  1:745324/1‑150 (MQ=255)

                         aaTATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTa                                                                                                                              >  2:3949571/1‑150 (MQ=255)

                           tatTTCACTCGGTATTATCGACCTGTTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGACAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaa                                                                                                                            >  1:2160950/1‑150 (MQ=255)

                           tatTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaa                                                                                                                            <  2:1589388/150‑1 (MQ=255)

                           tatTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaa                                                                                                                            <  2:1365263/150‑1 (MQ=255)

                            gtttCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaaa                                                                                                                           >  2:2549222/2‑150 (MQ=255)

                            atTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaaa                                                                                                                           >  2:1995166/1‑150 (MQ=255)

                            atTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaaa                                                                                                                           <  1:2109933/150‑1 (MQ=255)

                            atTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaaa                                                                                                                           >  1:4134386/1‑150 (MQ=255)

                              ttCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTaaaaaa                                                                                                                         <  2:3022540/150‑1 (MQ=255)

                               tCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCTACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAAc                                                                                                                        >  1:1631689/1‑150 (MQ=255)

                                cACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACt                                                                                                                       >  1:2957105/1‑150 (MQ=255)

                                 aCTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACtt                                                                                                                      >  1:1288504/1‑150 (MQ=255)

                                   tCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCTACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTc                                                                                                                    <  1:4626245/150‑1 (MQ=255)

                                   tCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTc                                                                                                                    >  1:2808751/1‑150 (MQ=255)

                                     ggTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCg                                                                                                                  >  2:494299/1‑150 (MQ=255)

                                        attatCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTa                                                                                                               <  2:3076265/150‑1 (MQ=255)

                                        attatCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTa                                                                                                               >  1:3755821/1‑150 (MQ=255)

                                          tatCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACa                                                                                                             >  1:616254/1‑150 (MQ=255)

                                           atCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAg                                                                                                            >  1:4277986/1‑150 (MQ=255)

                                           atCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAg                                                                                                            >  1:1823026/1‑150 (MQ=255)

                                             cGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCa                                                                                                          >  2:1541447/1‑150 (MQ=255)

                                              gACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCAc                                                                                                         >  2:4451441/1‑150 (MQ=255)

                                                 cTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTgg                                                                                                      <  2:2161881/150‑1 (MQ=255)

                                                       tATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTgcgc                                                                                                >  2:1816551/1‑150 (MQ=255)

                                                            cGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTAc                                                                                           >  1:1542673/1‑150 (MQ=255)

                                                            cGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTAc                                                                                           >  1:2976074/1‑150 (MQ=255)

                                                              cacaCTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGa                                                                                         >  2:2384897/1‑150 (MQ=255)

                                                              cacaCTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGa                                                                                         >  1:1332458/1‑150 (MQ=255)

                                                              cacaCTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGa                                                                                         >  1:1927737/1‑150 (MQ=255)

                                                                cacTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGAtt                                                                                       >  1:1141191/1‑150 (MQ=255)

                                                                 acTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTa                                                                                      >  1:3100239/1‑150 (MQ=255)

                                                                  cTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTAt                                                                                     <  1:1600829/150‑1 (MQ=255)

                                                                   tGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCTACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATc                                                                                    >  1:3238230/1‑150 (MQ=255)

                                                                    ggCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCg                                                                                   <  1:4435045/150‑1 (MQ=255)

                                                                       aGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTg                                                                                >  1:3570295/1‑150 (MQ=255)

                                                                       aGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTg                                                                                >  2:2424679/1‑150 (MQ=255)

                                                                               aTTGCTCAGGATCTCGGTCAACAAACCGATATCTATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCg                                                                        >  1:2293859/1‑150 (MQ=255)

                                                                                ttGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCgc                                                                       >  1:3750701/1‑150 (MQ=255)

                                                                                 tGCTCAGGATCTCGGTCAACACACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCgct                                                                      <  2:4251074/150‑1 (MQ=255)

                                                                                 tGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCgct                                                                      <  2:1960418/150‑1 (MQ=255)

                                                                                   cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCgctgc                                                                    >  1:2530297/1‑150 (MQ=255)

                                                                                     cAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGctgctg                                                                  <  2:1134129/150‑1 (MQ=255)

                                                                                      aGGATCTCGGTCAACAAACAGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTgg                                                                 <  2:4401266/150‑1 (MQ=255)

                                                                                       ggATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGt                                                                <  1:2309327/150‑1 (MQ=255)

                                                                                       ggATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGt                                                                <  1:1435934/150‑1 (MQ=255)

                                                                                           ctcGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTg                                                            <  2:1529101/150‑1 (MQ=255)

                                                                                             cGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGtt                                                          >  1:2743365/1‑150 (MQ=255)

                                                                                              ggTCAACAAACCGATATCGAGATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGttt                                                         <  2:3544111/150‑1 (MQ=255)

                                                                                               gTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTa                                                        <  2:2671006/150‑1 (MQ=255)

                                                                                                tCAACAAACCGATATCGATATCGATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTAt                                                       <  2:3113412/150‑1 (MQ=255)

                                                                                                    caaACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTg                                                   <  2:2957105/150‑1 (MQ=255)

                                                                                                         cgatatcgatatCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTAtt                                              <  1:1081361/150‑1 (MQ=255)

                                                                                                          gatatcgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTc                                             >  1:4554780/1‑150 (MQ=255)

                                                                                                            tatcgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTg                                           <  1:3500135/150‑1 (MQ=255)

                                                                                                              tcgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGa                                         <  2:4541099/150‑1 (MQ=255)

                                                                                                              tcgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGa                                         <  2:4062579/150‑1 (MQ=255)

                                                                                                               cgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGAt                                        <  1:2470714/150‑1 (MQ=255)

                                                                                                               cgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGAt                                        >  1:1850290/1‑150 (MQ=255)

                                                                                                                gatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATc                                       >  2:4622405/1‑150 (MQ=255)

                                                                                                                gatatcTATCTCGACCGCCAGGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGGGCTGTTTATGCTGTTATTCTGGATc                                       >  2:682311/1‑150 (MQ=255)

                                                                                                                     ctatctCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCt                                  <  1:2182738/150‑1 (MQ=255)

                                                                                                                      tatctCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTg                                 >  2:1032161/1‑150 (MQ=255)

                                                                                                                       atctCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCCgg                                >  2:2622071/1‑150 (MQ=255)

                                                                                                                        tctcGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGa                               >  2:3290997/1‑150 (MQ=255)

                                                                                                                           cGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGTTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGatat                            >  1:847821/1‑150 (MQ=255)

                                                                                                                            gACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATg                           <  1:2549222/150‑1 (MQ=255)

                                                                                                                                 ccATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCt                      <  2:26094/150‑1 (MQ=255)

                                                                                                                                    tGTAGTGCGTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGaa                   >  2:2305682/1‑150 (MQ=255)

                                                                                                                                            gTCAGGGACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCActc           <  2:3315940/150‑1 (MQ=255)

                                                                                                                                            gTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCActc           >  2:1833978/1‑150 (MQ=255)

                                                                                                                                                gggACTATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACtctctc       >  2:3615382/1‑150 (MQ=255)

                                                                                                                                                gggACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACtctctc       >  2:2361770/1‑150 (MQ=255)

                                                                                                                                                gggACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACtctctc       >  2:1369919/1‑150 (MQ=255)

                                                                                                                                                 ggACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCa      >  2:2877797/1‑150 (MQ=255)

                                                                                                                                                 ggACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCa      >  2:2638809/1‑150 (MQ=255)

                                                                                                                                                 ggACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTGTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCa      >  2:3938650/1‑150 (MQ=255)

                                                                                                                                                  gACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCAt     <  2:2809417/150‑1 (MQ=255)

                                                                                                                                                     gATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGa  >  1:3604679/1‑150 (MQ=255)

                                                                                                                                                     gATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGa  >  2:2918265/1‑150 (MQ=255)

CGAAAACGATCAGGAACAGATCAACAATATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGA  >  CP009273/3520640‑3520938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`