Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,232 T→A 10.4% L189F (TTA→TTT)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,2320TA10.4% 706.1 / 9.1 235L189F (TTA→TTT) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (59/141);  new base A (29/3);  total (91/144)
Fisher's exact test for biased strand distribution p-value = 3.84e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

GTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATG  >  CP009273/3525083‑3525381
                                                                                                                                                     |                                                                                                                                                      
 tAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTa                                                                                                                                                       <  1:4250619/150‑1 (MQ=255)
  aaaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTaa                                                                                                                                                      <  1:4362187/150‑1 (MQ=255)
    aCGGCAGGGTGATGTTAAACATCAGGGTTAGATATGATACTAACGGCATCAACCACTTGGCGATTAACGTTAACAACTTTTTAATTGAGTCTATTATACGTGTCTGTGTTCGCGCGAGGATCACCGACAGCGCGGTAACCAGACCTAACg                                                                                                                                                    <  2:3649601/150‑1 (MQ=255)
    aCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACg                                                                                                                                                    <  2:4265758/150‑1 (MQ=255)
     cGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAACGGGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGGAACCCGACCAAACGt                                                                                                                                                   >  2:3257092/1‑150 (MQ=255)
     cGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAACCCGACCAAACGt                                                                                                                                                   >  2:884209/1‑150 (MQ=255)
      ggCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTg                                                                                                                                                  <  1:2511716/150‑1 (MQ=255)
      ggCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCCCCAGCGCGGTAACCCGACCTAACCTg                                                                                                                                                  >  1:2722836/1‑150 (MQ=255)
        cAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAAAGTGTCTGTGTTGGCGCGAGGATCACCCCCAGCGCGGGAACCCGACCTAACGTggg                                                                                                                                                >  2:47041/1‑148 (MQ=255)
        cAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTgag                                                                                                                                                <  2:3457081/150‑1 (MQ=255)
        cAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTgag                                                                                                                                                <  2:2324334/150‑1 (MQ=255)
           ggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGata                                                                                                                                             <  2:2914374/150‑1 (MQ=255)
                  atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAAc                                                                                                                                      <  2:4708921/150‑1 (MQ=255)
                  atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAAc                                                                                                                                      <  2:2330025/150‑1 (MQ=255)
                    aaaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGGCAGTGTTCGCGCGAGGATCACCCCCAGCGCGGGAACCACACCTAACGTGAGATAAAAAAAACa                                                                                                                                    >  2:2793264/1‑150 (MQ=255)
                     aaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAg                                                                                                                                   <  1:1397258/150‑1 (MQ=255)
                     aaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAg                                                                                                                                   <  1:4650305/150‑1 (MQ=255)
                       cATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTATGTTCGCGCGAGGATAACCCCCCGCGCGGTAACCAGACCAAACCTGAGATAAATAAACCAGTc                                                                                                                                 >  1:4685695/1‑150 (MQ=255)
                       cATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACCCTACATTTTTCCCGCGATGAAAACCCACAGCGCCGGAACCAAACCTAACCTGTAATAAAAAAAACacaa                                                                                                                                 >  1:159209/1‑147 (MQ=255)
                        aTCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGTTTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCg                                                                                                                                <  1:1114652/150‑1 (MQ=255)
                        aTCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGACTAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCg                                                                                                                                <  1:2637575/150‑1 (MQ=255)
                        aTCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCg                                                                                                                                <  1:2637597/150‑1 (MQ=255)
                           aGGGTTAGCTATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCTATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCTGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGtt                                                                                                                             <  1:2723888/150‑1 (MQ=255)
                                tAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAAAGTGACTGTGTTCGCGCGGGGATAACCGCCAGCGCGGTAAACAGACCAAACGTGAGTTAAATAAAACAGTCGGGTGGaaa                                                                                                                        >  1:1770667/1‑150 (MQ=255)
                                tAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGTATGTGTTCGCGCGAGGATCACCCCCCGCGCGGTAACCAGACCAAAAGTGTTATAAATAAACCAGTAGGTTTCaaa                                                                                                                        >  1:1770679/1‑150 (MQ=255)
                                tAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACCTGACGGTGGTCGCGCGAGGCACACCCCCCCCCCCGGAACCCCACCAAACCGGAGATAAAAAAACCCCTCGGTTGCaaa                                                                                                                        >  1:1769970/1‑150 (MQ=255)
                                 aGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCAAACGTGAGATAAATAAACCAGTCGGTTGCaaaa                                                                                                                       >  2:3826263/1‑150 (MQ=255)
                                    aaTGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTAGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAag                                                                                                                    >  2:131686/1‑150 (MQ=255)
                                      tGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAagag                                                                                                                  <  2:4425297/150‑1 (MQ=255)
                                       gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGc                                                                                                                 <  1:3489566/150‑1 (MQ=255)
                                       gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGc                                                                                                                 <  1:2264970/150‑1 (MQ=255)
                                       gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCAAACGTGAGATAAATAAAACACTCGGTTGGaaaaaataac                                                                                                                 >  1:1803417/1‑146 (MQ=255)
                                       gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTTACTGTGTTCGCGCGCGGATCACCCCCAGCGCGGTAAACAGACCTAAAGTGAGATAAAAAAACCACACCGGTGCCAAAAAGAGc                                                                                                                 >  1:4191133/1‑150 (MQ=255)
                                         tACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑Gt                                                                                                              <  2:1017861/150‑1 (MQ=255)
                                         tACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑Gt                                                                                                              <  1:3164592/150‑1 (MQ=255)
                                          aCTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGTATGTGTTCGCGCGAGGTTCAACGCCAGCGCGGTAACCAGACCAAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑gga                                                                                                             >  2:3164794/1‑148 (MQ=255)
                                           cTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGTCGGTGTTCGCGCGAGGATAACCGCCCGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGGTGCCAAAAAGAGC‑GTAt                                                                                                            >  2:2223071/1‑150 (MQ=255)
                                            tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTAtt                                                                                                           <  1:2245949/150‑1 (MQ=255)
                                            tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTAtt                                                                                                           <  1:2406859/150‑1 (MQ=255)
                                             aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTg                                                                                                          <  1:3791128/150‑1 (MQ=255)
                                             aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGACGGTGGTCGCGCGCGGATCACCCCCCGCGCGGTAAACACACCAAACCAGAGATAAATAAAACAGTCGGGTGGAAAAAACAGC‑GGATTg                                                                                                          >  1:2949393/1‑150 (MQ=255)
                                              aCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGa                                                                                                         <  1:655907/150‑1 (MQ=255)
                                                ggCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACAGTGTTCGCGCGAGGATCACCCCCAGCGCGGTAACCAGACCTAACCTGAGATAAATAAACCAGTCGGTTTGAAAAAAAAGA‑CTATTGaaa                                                                                                       >  1:2069261/1‑150 (MQ=255)
                                                      aaCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTg                                                                                                 <  2:3592260/150‑1 (MQ=255)
                                                      aaCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTg                                                                                                 <  2:2041439/150‑1 (MQ=255)
                                                       aCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGGGACTGTGTTCGCGCGAGGATCACCGCCCCCGCGGTAAACCGACCAAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTTAAAAACGTGa                                                                                                >  1:3799640/1‑150 (MQ=255)
                                                         cccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAAAGGGGCTGTGTTAGCGCGCGGCTAACCCCCCCCGCGGTAACCAGAACTAAAGTGAGATAAAAAAAACACTCGGGTTGAAAAAATAGCGGTTTTGAAAAActgatt                                                                                               >  1:2407808/1‑145 (MQ=255)
                                                         cccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGGGACAGTGTTCGCGCGAGGATCACCGCCACCGCGGTAACCACACCTAACGTGAGATAAATAAAACACTCGGGTGCAAAAAAGAGC‑GGATTTAAAAAACTGAtt                                                                                              >  1:3547948/1‑149 (MQ=255)
                                                            gTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAACTGACGGTGTTCGCGCGAGGATCACCCCCCGCGCGGTAACCACACCAAAACTGAGATAAATAAACCACTCGGGTGCAAAAAAGAGC‑GGATTGAAAAAAGTGATGccc                                                                                           >  1:1927312/1‑149 (MQ=255)
                                                             tGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGGGGCTGTGTTCGCGCCAGGATCACCGCCCGCCCGGTAACCCGACCGAACGGGAGATAAATAAAACCGGAGGGTGCAAAAAAAAGC‑CGATTGAAAAAACTGTTGCcaa                                                                                          >  2:460043/1‑150 (MQ=255)
                                                               gCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCcaaca                                                                                        <  2:752807/150‑1 (MQ=255)
                                                                 gATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTTTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCGAACGTGTGATAAAAAAAACAGTCGGTTGCAAAAAAGAGC‑GGATTGAAAAAAGTTATTCcaccaaa                                                                                      >  2:930325/1‑149 (MQ=255)
                                                                 gATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATAACCCCCCGCGCCGTAAACAGACCAAACCTGAGATAAATAAAACCCTCCGTTGCAAAAAAGAGC‑CGATTGAAAAACCTGGTGCcaacaaa                                                                                      >  1:3871282/1‑149 (MQ=255)
                                                                    tAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGttt                                                                                   <  2:668387/150‑1 (MQ=255)
                                                                     aaCGTGAACAACTTTTGAATAGAGTCGATTAAACCTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGGAACCAGACCTAACGTGAGATAAATAAAACAGTCGGTTGCAAAAAAAAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTa                                                                                  >  2:2244380/1‑150 (MQ=255)
                                                                      aCGTGAACAACTTTGGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTaa                                                                                 <  2:3252487/150‑1 (MQ=255)
                                                                       cGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTaaa                                                                                <  2:3672573/150‑1 (MQ=255)
                                                                         tGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAaaca                                                                              <  1:3952776/150‑1 (MQ=255)
                                                                          gAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAaacaa                                                                             <  1:1222130/150‑1 (MQ=255)
                                                                            acaacTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAact                                                                           <  2:1493126/150‑1 (MQ=255)
                                                                            acaacTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAact                                                                           <  2:2346168/150‑1 (MQ=255)
                                                                                   ttGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCca                                                                    <  2:111529/150‑1 (MQ=255)
                                                                                     gAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGTTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCcaca                                                                  <  2:2014795/150‑1 (MQ=255)
                                                                                         attgTCTATTAACGGTGAGTGTGTTCGCGCGTGGATCACCGCCAGGGCGTTAACCAGCCCTACCTTTAGATAAATAACCCCGTCGGTTGCAAAAAAGAGC‑GTATTTAAAAACGTGATGCCAACAATTTTAAACAACTCACTCCACAGTaa                                                              <  1:3643161/147‑1 (MQ=255)
                                                                                         agagTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTaa                                                              <  2:2696697/150‑1 (MQ=255)
                                                                                            gTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAg                                                           <  1:4164333/150‑1 (MQ=255)
                                                                                            gTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAg                                                           <  1:1087647/150‑1 (MQ=255)
                                                                                                    aaCGTTACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACAATACATAACTTTAGATAAATAAACCAGTCGGTTGAAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAAAAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGcc                                                   <  2:3606377/150‑1 (MQ=255)
                                                                                                     aCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCa                                                  <  1:1966984/150‑1 (MQ=255)
                                                                                                          aCTGTGTTCGCGCGTGGATCACGGCCTGCGCGGTACCCAGACCTAACGTTAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCa                                             <  1:3325789/150‑1 (MQ=255)
                                                                                                           cTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                            >  2:1811655/1‑150 (MQ=255)
                                                                                                           cTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                            >  2:2654483/1‑150 (MQ=255)
                                                                                                           cTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                            >  2:3275968/1‑150 (MQ=255)
                                                                                                           cTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                            >  2:3957415/1‑150 (MQ=255)
                                                                                                               gtTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCa                                        <  2:3779853/150‑1 (MQ=255)
                                                                                                                  cgcgcgAGGATCACCGCCTGCGCGGTAACCAGACCTACCGTGAGATAAATAAACCCGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTg                                     <  1:2168702/150‑1 (MQ=255)
                                                                                                                        aGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgaga                               >  1:1428168/1‑150 (MQ=255)
                                                                                                                         ggATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaa                              <  2:4175077/150‑1 (MQ=255)
                                                                                                                          gATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaa                             >  2:745952/1‑150 (MQ=255)
                                                                                                                           aTCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaaa                            <  1:2548476/150‑1 (MQ=255)
                                                                                                                             cACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAAt                          <  1:1450665/150‑1 (MQ=255)
                                                                                                                             cACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAAt                          <  2:2112359/150‑1 (MQ=255)
                                                                                                                             cACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAAt                          <  2:3934801/150‑1 (MQ=255)
                                                                                                                               ccGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCa                        >  1:2171590/1‑150 (MQ=255)
                                                                                                                                 gCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAcc                      >  2:708243/1‑150 (MQ=255)
                                                                                                                                 gCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAcc                      >  2:1861547/1‑150 (MQ=255)
                                                                                                                                   cAGCGCGGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                    <  1:3428900/150‑1 (MQ=255)
                                                                                                                                   cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                    >  2:574884/1‑150 (MQ=255)
                                                                                                                                   cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                    <  2:838823/150‑1 (MQ=255)
                                                                                                                                      cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                 >  2:3095990/1‑150 (MQ=255)
                                                                                                                                        cgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAata               <  2:3008712/150‑1 (MQ=255)
                                                                                                                                         tgTAACCAGACCTTAAGTGAGATAAATAAACCATGCTGTTGCAATAAAGAGA‑GTATTGATATTCTTTATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCTGCCACGTCAAGCCATTGGCGTGAAAAATCTCCAGTAattt              <  2:159209/149‑3 (MQ=255)
                                                                                                                                         ggTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAatat              >  2:1746961/1‑150 (MQ=255)
                                                                                                                                         ggTAAACAGACCTAACGTGAGATAAATAAAACAGTAGGAAGCAAAAAAGAGC‑GTATTGAAAAACGTGAAGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAACAGCCACGACAAGCCATAGGAGAGAAAAAAAACCAGTAatat              >  2:4131658/1‑150 (MQ=255)
                                                                                                                                          gTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAAtata             <  2:4469558/150‑1 (MQ=255)
                                                                                                                                            aaCCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAg           >  2:1674170/1‑150 (MQ=255)
                                                                                                                                             aCCAGACCTAACGTGAGATAAAAACACCAGTCGGTTGAAAAAAAGAGC‑GTATTGAAAAACGTGATGCAAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGt          <  1:458338/150‑1 (MQ=255)
                                                                                                                                             aCCAGACATACCGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGt          <  1:4623958/150‑1 (MQ=255)
                                                                                                                                                   ccTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTa    <  2:3830071/150‑1 (MQ=255)
                                                                                                                                                     tAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCCGTAATATAAGTACGTTATg  >  1:831778/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGC‑GTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATG  >  CP009273/3525083‑3525381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑