Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,480 T→G 9.2% T418P (ACG→CCG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4800TG 9.2% 709.3 / 6.4 276T418P (ACG→CCG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (96/145);  new base G (32/3);  total (128/148)
Fisher's exact test for biased strand distribution p-value = 4.64e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  CP009273/3721332‑3721629
                                                                                                                                                    |                                                                                                                                                     
 gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTAc                                                                                                                                                     <  1:467310/150‑1 (MQ=255)
     aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCggga                                                                                                                                                 >  1:3064583/1‑148 (MQ=255)
         gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCg                                                                                                                                             <  1:1026103/150‑1 (MQ=255)
         gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCg                                                                                                                                             <  1:3670597/150‑1 (MQ=255)
          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTTATCGa                                                                                                                                            <  2:1932622/150‑1 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAg                                                                                                                                           <  2:1758928/150‑1 (MQ=255)
              aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGACGGGCCTCGAgctg                                                                                                                                        >  1:3538269/1‑150 (MQ=255)
              aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                        <  2:888898/150‑1 (MQ=255)
                    cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGTCCTCGGgctgggggcc                                                                                                                                  >  1:2977757/1‑144 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCGTCGGGCTGCTGACCGcg                                                                                                                               >  2:3052153/1‑149 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                            >  2:2397992/1‑150 (MQ=255)
                           tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGGAATCGAGCGGCGGACCGcgggga                                                                                                                           >  1:1706876/1‑145 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                          >  2:1847467/1‑150 (MQ=255)
                             tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                                                         <  1:628380/150‑1 (MQ=255)
                              ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                        <  2:246852/150‑1 (MQ=255)
                               gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTGCGGTAATCGGGCTGCGGTCCGCTgggggccg                                                                                                                       >  1:1041693/1‑143 (MQ=255)
                               gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                                                       <  2:2760274/150‑1 (MQ=255)
                                  gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                    <  1:3586392/150‑1 (MQ=255)
                                   aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                   <  2:2900520/150‑1 (MQ=255)
                                     gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                                                                                                 <  2:4633305/150‑1 (MQ=255)
                                      cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                <  2:2921533/150‑1 (MQ=255)
                                          tGTTCTACCGGTAGTGGCGGCAACAATTCAAGGCGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                            <  2:2546432/150‑1 (MQ=255)
                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                        <  1:4066219/150‑1 (MQ=255)
                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                        <  1:2021295/150‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                                  <  1:3339037/150‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTGCCCCCCCGTCCGGGAGTCGAGCTGCGGACCGCGGCTAGGCGCCCGCGGCTGCCGCCCGtggtca                                                                                                >  2:3250539/1‑145 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                              <  1:3957554/150‑1 (MQ=255)
                                                        gtGCGGCAACAATTCAATGAGCGCTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                              <  1:1610037/149‑1 (MQ=255)
                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGGAAGCGAGCTGCTGGCCGCGGGGGGCCGCCAGCAGCTGGCGGCAGTACTCACTAcg                                                                                           >  1:2171889/1‑150 (MQ=255)
                                                            ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGCATCGCGCTGCTGTCCGCGGATATCCGCCAGCCTCTGACGCCCGTACTCCCGAcgc                                                                                          >  2:2668301/1‑150 (MQ=255)
                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGGCCGCGGAGATCCGCCAGCAGCGGGCGGCCGGACTCACTGCGcgcc                                                                                       >  2:4342016/1‑150 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATTTCCGCCCGCATCTGACGCCAGTACTCACTTCGcgccccgcc                                                                                  >  1:3530838/1‑150 (MQ=255)
                                                                     tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGGAATCGAGCTGCTGGCCGCTGATATCCGCCCGCCGCTGGCGCCCGGTCTCGCGCCGcgccccgcgc                                                                                 >  2:818364/1‑148 (MQ=255)
                                                                       aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCCCCCCCGTACGGTAATCGAGCTGCGGAGCGGTGATAGCCGCCAGCGTCTGACGCCAGTTCTCACTAcgcgccccgcgccc                                                                               >  1:1552336/1‑150 (MQ=255)
                                                                        aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTGCGGGAATCGAGCTGCTGACCGCGGAGAGCCGCCAGCATCTGACGCCAGGACTCACTCCGCGCCCCGCCCCCa                                                                              >  1:1387635/1‑150 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                                          <  1:3196938/150‑1 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                                          <  1:2297413/150‑1 (MQ=255)
                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                        <  1:4735026/150‑1 (MQ=255)
                                                                                tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                      <  1:1922888/150‑1 (MQ=255)
                                                                                 ttCTCTGGATTCGCCGCGATCTGCGCTAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACTCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                     <  1:99268/150‑1 (MQ=255)
                                                                                 ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                     <  2:425023/150‑1 (MQ=255)
                                                                                   ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                   <  2:1336995/150‑1 (MQ=255)
                                                                                     ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacac                                                                 <  1:2702679/150‑1 (MQ=255)
                                                                                      tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                <  1:3652734/150‑1 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                               <  1:2816050/150‑1 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                               <  1:2397992/150‑1 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGCCGGGTATCGGGCTGGGGGGCGCTGATATCCGCCAGCATGTGGCGCCAGGTCTGACTACGCGCCCCGCCCCCAATCCACGTGACACtt                                                               >  1:4244196/1‑150 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGAAGCGAGCTGCTGACCGCTGGGAGCCGCCAGCATCTGACGCCAGTACGCACGACGCGCCCCGCCCCCACTCGACGGAacacgt                                                               >  1:2920131/1‑148 (MQ=255)
                                                                                        gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGGAACACgtt                                                              >  1:1790916/1‑150 (MQ=255)
                                                                                            cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGTACTCGAGCTGCTGACCGCGGAGATCCGCCAGCATCTGACGCCAGTCCTCACTCCGCGCCCCGCCCCCACTCAACGTAACACGTTGCgg                                                          >  1:844037/1‑150 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGATCGAGCTGCTGGCCGCTGGTATCCGCCCGCAGCTGCCGCCAGGACTCACTACGCGCCCCGCCCCCAAGCACCGTCACACGTTGCGGt                                                         >  2:2959229/1‑150 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                        <  2:4199018/150‑1 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                        <  2:733857/150‑1 (MQ=255)
                                                                                               cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCggggt                                                       >  1:1081703/1‑147 (MQ=255)
                                                                                               cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                       <  2:3988039/150‑1 (MQ=255)
                                                                                                gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                      <  1:763510/150‑1 (MQ=255)
                                                                                                gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGATGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                      <  1:97066/150‑1 (MQ=255)
                                                                                                 cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGGGGGACTCGAGCTGCTGACCGGGGGTATCCGCCAGCGTCTGCCGGCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACGCGTTGCGGTTTaa                                                     >  2:2018494/1‑150 (MQ=255)
                                                                                                 cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                     <  2:789954/150‑1 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGTAATCGAGCTGCTGACCGGGGATATCCGCCAGCAGCTGACGCCCGTACTCTCTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCgggtgatt                                                    >  2:1119900/1‑144 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGATGAGCGCTGATATGCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCGCTCAACGTACCACTTTGCGGTTTAAt                                                    >  2:1394039/1‑150 (MQ=255)
                                                                                                    tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                  <  1:2576655/150‑1 (MQ=255)
                                                                                                     cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGTACTCGCGGTGCTGACCGCTGATCTCCGCCAGCATCTGCCGCCAGGACTCCCGACGCGCCCCGCCCCCCATCAACGTCCCACTGTGCGGTTTGATAcc                                                 >  2:1589759/1‑150 (MQ=255)
                                                                                                     cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                 <  1:4316335/150‑1 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGTAATCGAGCTGCTGACCGCGGCTCTCCGCCCGCATCTGAGGCCCGTACGCCCGACGCGCCCCGCCCCCCAGCCACGGAACACTGTGCGGGTGAATACCGcc                                              >  2:4519407/1‑149 (MQ=255)
                                                                                                          ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGACGGGAATCGAGCGGCTGTCCGCGGGTATCCGCCAGCCTCTGACGCCAGGACTCACTACGCGCCCCGCCCCCCAGCAACGGAACCCGTTGCGGGGTCATACCGCAgg                                            >  1:1523825/1‑150 (MQ=255)
                                                                                                           cAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGGGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGTACACGTTGCGGTGTAATACCGCAGGc                                           >  1:273804/1‑150 (MQ=255)
                                                                                                            aGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGGACTCGTGCGGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGGAACACTTTGGGGGTTAATACCGCAGGCa                                          >  1:1582391/1‑150 (MQ=255)
                                                                                                             gCCTTGCTGCGCCCAGTGCTTGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                         <  1:1363974/150‑1 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCGGTTATCCGCCAGCATCTGACGCCAGTTCTCACTACGCGCCCCGCCCCCAAGCAACGTTACACTTTGCGGTGTGTTACCGCAGGCATg                                        >  1:903112/1‑150 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                        <  2:17517/150‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg                                    <  1:343746/150‑1 (MQ=255)
                                                                                                                   ctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                   <  1:4425662/150‑1 (MQ=255)
                                                                                                                    tgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCCACGTAACACTTTGCGGGTTAATACCGCAGGCCTGCACGAc                                  >  2:3570523/1‑150 (MQ=255)
                                                                                                                     gcgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                 <  1:778601/150‑1 (MQ=255)
                                                                                                                       gcCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                               <  1:1290440/150‑1 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACATCCCCCCCCGTACGTTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCACAATCAACGTAACACTTTGCGGTTTAATACCGCATGCATGCACGACATcc                              <  2:3622383/150‑1 (MQ=255)
                                                                                                                         ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGAGATCCGCCAGCAGCTGACGCCCGTTCTCGCTACGCGCCCCGCCCCCCATCAACGGAACACTTTGCGGGTTAATTCCGCAGGCAGGCGCGGCAGCCa                             >  2:3141234/1‑150 (MQ=255)
                                                                                                                          cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                            <  2:1411122/150‑1 (MQ=255)
                                                                                                                          cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCCGCATCTGTCGCCAGTACTCACGACGCGCCCCGCCCCCCATCAACGTAACAGTTTGCGGTTGATTACCGCAGGCATGGACGACATCCAt                            >  1:114241/1‑150 (MQ=255)
                                                                                                                           aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                           <  1:1339259/150‑1 (MQ=255)
                                                                                                                           aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                           >  1:1981095/1‑150 (MQ=255)
                                                                                                                                 ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCAGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAACGCAGGCATGCACGACATCCATGCCATCt                     >  2:1697152/1‑150 (MQ=255)
                                                                                                                                  gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                    <  2:4295578/150‑1 (MQ=255)
                                                                                                                                    cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTATTAACGCAGGCATGCAAGACATCCATGCCATCTGcc                  >  2:1096284/1‑150 (MQ=255)
                                                                                                                                    cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                  >  2:2348170/1‑150 (MQ=255)
                                                                                                                                       acaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc               <  2:3446301/150‑1 (MQ=255)
                                                                                                                                       acaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc               <  1:3928459/150‑1 (MQ=255)
                                                                                                                                         aTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc             >  2:3201561/1‑150 (MQ=255)
                                                                                                                                          tCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa            >  2:2107688/1‑150 (MQ=255)
                                                                                                                                           ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt           <  1:683490/150‑1 (MQ=255)
                                                                                                                                            cccccccGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa          <  2:3043454/150‑1 (MQ=255)
                                                                                                                                            cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa          <  1:2862589/150‑1 (MQ=255)
                                                                                                                                            cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa          <  2:2000742/150‑1 (MQ=255)
                                                                                                                                             ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa         <  1:4004110/150‑1 (MQ=255)
                                                                                                                                              cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc        >  2:532375/1‑150 (MQ=255)
                                                                                                                                                 ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa     <  2:3752290/150‑1 (MQ=255)
                                                                                                                                                 ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa     <  2:3754294/150‑1 (MQ=255)
                                                                                                                                                  cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAc    >  1:3873268/1‑150 (MQ=255)
                                                                                                                                                    tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc  >  1:3466639/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  CP009273/3721332‑3721629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑