BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,926,496	A→C	5.4%	E607D (GAA→GAC)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,926,496	0	A	C	5.4%	803.2 / 31.9	241	E607D (GAA→GAC)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base A (85/142); new base C (4/9); total (89/152)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 243 total aligned reads displayed.

GGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAT  >  CP009273/3926350‑3926645

ggATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATc                                                                                                                                                    <  1:2295684/150‑1 (MQ=255)

    tAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCCCCAGATCAATTTGAAATCCCGc                                                                                                                                                >  1:1457653/1‑150 (MQ=255)

      aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTGTTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCa                                                                                                                                              <  2:1093459/150‑1 (MQ=255)

      aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCa                                                                                                                                              <  1:1024345/150‑1 (MQ=255)

      aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCa                                                                                                                                              <  1:3797243/150‑1 (MQ=255)

      aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCAGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCa                                                                                                                                              >  2:3801838/1‑150 (MQ=255)

       gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCaa                                                                                                                                             >  1:2597538/1‑150 (MQ=255)

        ttGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCaaa                                                                                                                                            >  2:1276183/1‑150 (MQ=255)

        ttGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCaaa                                                                                                                                            >  2:1276131/1‑150 (MQ=255)

           ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAg                                                                                                                                         <  1:3168579/150‑1 (MQ=255)

           ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAAAGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAg                                                                                                                                         >  1:4547307/1‑150 (MQ=255)

              gatgatGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGt                                                                                                                                      <  2:828241/150‑1 (MQ=255)

               atgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGtt                                                                                                                                     <  1:1753004/150‑1 (MQ=255)

                  atgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATc                                                                                                                                  >  1:3024783/1‑150 (MQ=255)

                   tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCg                                                                                                                                 <  2:1745350/150‑1 (MQ=255)

                   tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCg                                                                                                                                 >  1:3933321/1‑150 (MQ=255)

                      aaaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAAc                                                                                                                              >  2:3070813/1‑150 (MQ=255)

                         cctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTgg                                                                                                                           <  1:3498805/150‑1 (MQ=255)

                          ctcctTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTggg                                                                                                                          >  1:308061/1‑150 (MQ=255)

                                cttTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCTCCAAACAGGGTTATCGAACTGGGTACTCa                                                                                                                    <  2:331528/150‑1 (MQ=255)

                                cttTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCTCCAAACAGGGTTATCGAACTGGGTACTCa                                                                                                                    <  2:234523/150‑1 (MQ=255)

                                  ttATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAgg                                                                                                                  <  1:1341766/150‑1 (MQ=255)

                                  ttATGTCGCATGAGTCGTTGATACTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACATGCTGCTGCAACGTAAAGCGCTGCGTGCACCACATCAATTTTAAATACCGCCAAACAGGGTTATCGAACTGGGTACTCAgg                                                                                                                  >  1:1998712/1‑150 (MQ=255)

                                   tATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAAAAGGGTTATCGAACTGGGTACTCAGGt                                                                                                                 >  2:3210700/1‑150 (MQ=255)

                                    aTGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTc                                                                                                                <  1:1760233/150‑1 (MQ=255)

                                     tGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCg                                                                                                               <  1:775241/150‑1 (MQ=255)

                                       tCGCATGAGTCGTTGATCCTCGGCAAACGTCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGaa                                                                                                             <  1:954482/150‑1 (MQ=255)

                                       tCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGaa                                                                                                             >  2:2728518/1‑150 (MQ=255)

                                         gCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAAt                                                                                                           <  2:231078/150‑1 (MQ=255)

                                          cATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATc                                                                                                          <  1:4313265/150‑1 (MQ=255)

                                           aTGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCt                                                                                                         <  1:3696680/150‑1 (MQ=255)

                                            tGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTTCTGCTGCAACGTAATTCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTa                                                                                                        >  1:1133814/1‑150 (MQ=255)

                                                 cGTTGATCCTCGGCACACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGcc                                                                                                   <  1:1640044/150‑1 (MQ=255)

                                                  gTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCa                                                                                                  >  2:2638298/1‑150 (MQ=255)

                                                   ttGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAg                                                                                                 <  1:265421/150‑1 (MQ=255)

                                                   ttGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAg                                                                                                 >  2:718322/1‑150 (MQ=255)

                                                    tGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGa                                                                                                <  2:2083995/150‑1 (MQ=255)

                                                    tGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGa                                                                                                >  1:3133816/1‑150 (MQ=255)

                                                      aTCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACg                                                                                              >  2:3026708/1‑150 (MQ=255)

                                                        ccTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGTGTACTCAGGTCGAAATCTAACGCCAGACGcc                                                                                            >  1:4009276/1‑150 (MQ=255)

                                                        ccTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcc                                                                                            >  1:680462/1‑150 (MQ=255)

                                                         cTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcct                                                                                           <  1:1093576/150‑1 (MQ=255)

                                                         cTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcct                                                                                           <  1:2217280/150‑1 (MQ=255)

                                                              cAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCttt                                                                                      <  1:606802/150‑1 (MQ=255)

                                                               aaaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttc                                                                                     >  1:3993260/1‑150 (MQ=255)

                                                               aaaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttc                                                                                     <  2:4614989/150‑1 (MQ=255)

                                                                aaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttct                                                                                    <  1:4565331/150‑1 (MQ=255)

                                                                 aCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttctt                                                                                   <  2:4274283/150‑1 (MQ=255)

                                                                 aCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttctt                                                                                   >  1:2342880/1‑150 (MQ=255)

                                                                    cccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAt                                                                                <  1:718358/150‑1 (MQ=255)

                                                                    cccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAt                                                                                <  2:4527721/150‑1 (MQ=255)

                                                                      cGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATaa                                                                              <  1:3466821/150‑1 (MQ=255)

                                                                           tATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAggggg                                                                         >  1:2480003/1‑150 (MQ=255)

                                                                           tATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAggggg                                                                         <  2:2541671/150‑1 (MQ=255)

                                                                           tATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAGAAggggg                                                                         >  1:4067048/1‑150 (MQ=255)

                                                                            aTTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATAAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGc                                                                        <  1:839971/150‑1 (MQ=255)

                                                                             tttGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCg                                                                       <  2:2453518/150‑1 (MQ=255)

                                                                             tttGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCg                                                                       <  2:1965231/150‑1 (MQ=255)

                                                                               tGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGtt                                                                     <  2:4545195/150‑1 (MQ=255)

                                                                               tGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGtt                                                                     <  2:1293513/150‑1 (MQ=255)

                                                                                       cgcgGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGtttt                                                             <  1:2057197/150‑1 (MQ=255)

                                                                                          ggCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCAt                                                          <  2:536282/150‑1 (MQ=255)

                                                                                           gCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATg                                                         <  2:194299/150‑1 (MQ=255)

                                                                                            cAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAGCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATgg                                                        >  2:2596489/1‑150 (MQ=255)

                                                                                             aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                       >  1:3035801/1‑150 (MQ=255)

                                                                                             aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                       >  1:1423225/1‑150 (MQ=255)

                                                                                             aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                       >  2:1504763/1‑150 (MQ=255)

                                                                                             aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                       <  1:4036390/150‑1 (MQ=255)

                                                                                               gCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTa                                                     <  2:2992798/150‑1 (MQ=255)

                                                                                               gCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTa                                                     <  2:3618214/150‑1 (MQ=255)

                                                                                                   tACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCa                                                 <  1:2903242/150‑1 (MQ=255)

                                                                                                   tACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGATTTTGTTTTCATGGTTAATCa                                                 <  2:396249/150‑1 (MQ=255)

                                                                                                   tACTTGCTGCTGAAACGTAATGCGCTGCTTTCACAAGATCATTTTGATATCCCTCCAAAGAGTGTTATCGAACTGGGTACTGAGGTCGAAATCTAACGCCAGACGCCTGCTTTGGTCATAAGGGGTCGTTTTTGTTTTCATGTTTAATCa                                                 <  1:2557448/150‑1 (MQ=255)

                                                                                                    aCTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCAc                                                <  2:1945663/150‑1 (MQ=255)

                                                                                                      ttGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCa                                              <  1:938647/150‑1 (MQ=255)

                                                                                                       tgctgctgcAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCAt                                             <  1:3786366/150‑1 (MQ=255)

                                                                                                          tgctgcAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTa                                          <  2:1612665/150‑1 (MQ=255)

                                                                                                             tgcAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTaaaa                                       >  2:753845/1‑150 (MQ=255)

                                                                                                               cAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACg                                     <  2:560416/150‑1 (MQ=255)

                                                                                                                  cGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGttt                                  <  2:2427822/150‑1 (MQ=255)

                                                                                                                  cGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGttt                                  <  1:3778924/150‑1 (MQ=255)

                                                                                                                    tAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCg                                <  2:2891519/150‑1 (MQ=255)

                                                                                                                    tAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCg                                <  1:2374492/150‑1 (MQ=255)

                                                                                                                      aTGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAg                              <  2:356046/150‑1 (MQ=255)

                                                                                                                       ggcgcAGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAgg                             <  2:849955/149‑1 (MQ=255)

                                                                                                                           cTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGATTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGa                         <  2:346170/150‑1 (MQ=255)

                                                                                                                            tGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGAt                        <  1:3975667/150‑1 (MQ=255)

                                                                                                                            tGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGAt                        <  2:4661653/150‑1 (MQ=255)

                                                                                                                              cGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGTAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATca                      <  2:4330425/150‑1 (MQ=255)

                                                                                                                                 gCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAt                   <  2:3892448/150‑1 (MQ=255)

                                                                                                                                      aGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCg              >  2:4621283/1‑150 (MQ=255)

                                                                                                                                           aaTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAGAAGGGGGCGTTTTTGTTTTCATGGGTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACg         >  2:2336510/1‑150 (MQ=255)

                                                                                                                                            aTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGt        >  1:1544343/1‑150 (MQ=255)

                                                                                                                                            aTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTACGTAACGt        >  1:4651935/1‑150 (MQ=255)

                                                                                                                                             tttGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCCTCAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGGAAAACGTTTCGAGGGTGCTCACATTTCCGGAACGTc       >  2:1591282/1‑150 (MQ=255)

                                                                                                                                             tttGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTc       <  1:3432842/150‑1 (MQ=255)

                                                                                                                                             tttGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTc       >  2:1591465/1‑150 (MQ=255)

                                                                                                                                              ttGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGTAAAAAGTTTAGAGGTTGTTCACATTTCCGTAACGTCa      >  1:3276781/1‑150 (MQ=255)

                                                                                                                                                 aaaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATAAACATGTAAAAAGTTTCGAGGTTGTTAACATTTCCGTAACGTCACGa   >  1:1940877/1‑150 (MQ=255)

                                                                                                                                                  aaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAt  <  2:2000263/150‑1 (MQ=255)

GGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAT  >  CP009273/3926350‑3926645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`