Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,932,727 C→A 17.2% V416F (GTT→TTT)  hsrA ← putative multidrug or homocysteine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,932,7270CA17.2% 683.2 / 95.1 245V416F (GTT→TTT) hsrAputative multidrug or homocysteine efflux system
Reads supporting (aligned to +/- strand):  ref base C (105/96);  new base A (31/13);  total (136/109)
Fisher's exact test for biased strand distribution p-value = 3.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

AGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCA  >  CP009273/3932578‑3932876
                                                                                                                                                     |                                                                                                                                                     
 gaattACGCTGTCTTTTTATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAcg                                                                                                                                                      <  2:2765783/147‑1 (MQ=255)
    ttACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGa                                                                                                                                                   >  2:2912334/1‑150 (MQ=255)
        gCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGa                                                                                                                                               <  1:3335743/150‑1 (MQ=255)
        gCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGa                                                                                                                                               <  2:1937367/150‑1 (MQ=255)
         cTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGAc                                                                                                                                              <  2:4442177/150‑1 (MQ=255)
         cTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGAc                                                                                                                                              >  2:2521720/1‑150 (MQ=255)
             cTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGcc                                                                                                                                          <  2:2147998/150‑1 (MQ=255)
              ttttGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCg                                                                                                                                         <  1:3244380/150‑1 (MQ=255)
              ttttGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCg                                                                                                                                         <  2:3794604/150‑1 (MQ=255)
                 tGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCAc                                                                                                                                      >  1:537594/1‑150 (MQ=255)
                  gATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACt                                                                                                                                     >  1:809663/1‑150 (MQ=255)
                   aTCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACtt                                                                                                                                    <  1:3865676/150‑1 (MQ=255)
                    tCAAATTATTACCTTCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGTAGCAACAGTAATAATGCCCATTGTGATAAACTTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTACTTAATAAACGCGAAGGACGGCCGCACTTa                                                                                                                                   <  2:1245453/150‑1 (MQ=255)
                     cAAGTTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTAc                                                                                                                                  <  2:3939410/150‑1 (MQ=255)
                       aaTTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAg                                                                                                                                <  2:3353985/150‑1 (MQ=255)
                       aaTTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAg                                                                                                                                <  1:2345765/150‑1 (MQ=255)
                       aaTTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAg                                                                                                                                <  1:2509791/150‑1 (MQ=255)
                          tattaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCaa                                                                                                                             >  1:4126662/1‑150 (MQ=255)
                            ttaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACg                                                                                                                           <  2:3192141/150‑1 (MQ=255)
                                cATCGGTTGTTTTCAGTAGCATGAACATTGCTGCTGAAGAAACAGTAATAATGCCCATTGTGATTAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTa                                                                                                                       <  1:782625/150‑1 (MQ=255)
                                cATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTa                                                                                                                       >  1:4410810/1‑150 (MQ=255)
                                 aTCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTaa                                                                                                                      >  2:2022623/1‑150 (MQ=255)
                                 aTCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTaa                                                                                                                      <  2:1103504/150‑1 (MQ=255)
                                 aTCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTaa                                                                                                                      >  1:104230/1‑150 (MQ=255)
                                  tCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTaaa                                                                                                                     <  1:2520968/150‑1 (MQ=255)
                                    ggTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACt                                                                                                                   >  2:514465/1‑150 (MQ=255)
                                    ggTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGAGAAGGACGGCCGCACTTACAGCAACGCCTAAACt                                                                                                                   >  1:1789619/1‑150 (MQ=255)
                                       tgttTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAAt                                                                                                                <  1:3097211/150‑1 (MQ=255)
                                       tgttTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAAt                                                                                                                <  1:730007/150‑1 (MQ=255)
                                           ttCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTGCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCACCGCCTAAACTAATCGAc                                                                                                            >  2:1830557/1‑150 (MQ=255)
                                              aGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGt                                                                                                         >  1:349861/1‑150 (MQ=255)
                                               gAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGtt                                                                                                        >  2:4366095/1‑150 (MQ=255)
                                                aaGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGATTTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtg                                                                                                       <  2:3973456/150‑1 (MQ=255)
                                                aaGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtg                                                                                                       >  2:2546817/1‑150 (MQ=255)
                                                aaGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtg                                                                                                       >  2:1520029/1‑150 (MQ=255)
                                                  gCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtgct                                                                                                     <  2:4291118/150‑1 (MQ=255)
                                                         taTTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                                                                              >  1:720227/2‑150 (MQ=255)
                                                         cATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                                                                              <  1:3912669/150‑1 (MQ=255)
                                                         cATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                                                                              <  1:1900341/150‑1 (MQ=255)
                                                         cATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                                                                              <  1:1841956/150‑1 (MQ=255)
                                                         cATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                                                                              <  1:3134912/150‑1 (MQ=255)
                                                         atttGCTGCTTAAGCAAAATTAATAATGCCCATTGTGATAAACTTATAGTGTATTTGTTCGACTGTCGTTGTGCCTTCCATTCATTAATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                                                                              <  1:1285399/148‑1 (MQ=255)
                                                              ctgctgAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCa                                                                                         >  2:195261/1‑150 (MQ=255)
                                                               tgctgAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAg                                                                                        >  1:658626/1‑150 (MQ=255)
                                                                  tgAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAac                                                                                     >  2:3430060/1‑150 (MQ=255)
                                                                    aaGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAacac                                                                                   >  1:1725508/1‑150 (MQ=255)
                                                                    aaGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATACCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATAGACAGTTGCTGCGTGACCGCCAGAacac                                                                                   >  2:3198693/1‑150 (MQ=255)
                                                                      gCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAACCGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTg                                                                                 <  2:4524642/150‑1 (MQ=255)
                                                                       cAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGATGCTGCGAGACCGCCAGAACACTGt                                                                                >  1:1129123/1‑150 (MQ=255)
                                                                          cAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTAc                                                                             <  2:702219/150‑1 (MQ=255)
                                                                              aataatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgct                                                                         >  2:393318/1‑150 (MQ=255)
                                                                               ataatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctg                                                                        >  2:471961/1‑150 (MQ=255)
                                                                                taatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgc                                                                       <  1:577604/150‑1 (MQ=255)
                                                                                taatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgc                                                                       >  2:965323/1‑150 (MQ=255)
                                                                                 aatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgct                                                                      <  2:1569437/150‑1 (MQ=255)
                                                                                 aatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgct                                                                      >  1:3710316/1‑150 (MQ=255)
                                                                                 aatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgct                                                                      <  2:3624935/150‑1 (MQ=255)
                                                                                 aatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgct                                                                      >  2:172315/1‑150 (MQ=255)
                                                                                      ccATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGATGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCAt                                                                 >  2:1996588/1‑150 (MQ=255)
                                                                                         ttGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGACGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGt                                                              <  1:449807/150‑1 (MQ=255)
                                                                                          tgtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTc                                                             <  2:3997910/150‑1 (MQ=255)
                                                                                          tgtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTc                                                             <  2:3707277/150‑1 (MQ=255)
                                                                                          tgtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTc                                                             >  2:2429801/1‑150 (MQ=255)
                                                                                          tgtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTc                                                             >  2:4268998/1‑150 (MQ=255)
                                                                                           gtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCa                                                            <  1:1520029/150‑1 (MQ=255)
                                                                                           gtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCa                                                            <  2:1690697/150‑1 (MQ=255)
                                                                                            tgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGACGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCAt                                                           <  1:508914/150‑1 (MQ=255)
                                                                                            tgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCAt                                                           <  2:1459267/150‑1 (MQ=255)
                                                                                            tgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCAt                                                           >  1:457365/1‑150 (MQ=255)
                                                                                             gATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATc                                                          <  1:2359079/150‑1 (MQ=255)
                                                                                               tAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCgg                                                        >  1:3277324/1‑150 (MQ=255)
                                                                                                    gTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGa                                                   >  2:274217/1‑150 (MQ=255)
                                                                                                     tataGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGAt                                                  <  2:1154885/150‑1 (MQ=255)
                                                                                                        tgTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCgg                                               <  1:3934602/149‑1 (MQ=255)
                                                                                                          tGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCa                                             <  1:4169238/150‑1 (MQ=255)
                                                                                                          tGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCa                                             <  1:2816739/150‑1 (MQ=255)
                                                                                                              aTTGTTCGACAGTCGTTGTGCCTTCCATTCCGTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAgtg                                         >  1:1198584/1‑150 (MQ=255)
                                                                                                               ttGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAgtgt                                        >  1:2583986/1‑150 (MQ=255)
                                                                                                               ttGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAgtgt                                        >  1:3165636/1‑150 (MQ=255)
                                                                                                                    cGACAGTCGTTGTGCCTTCCATTCCTTCATAAAATCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATgg                                   >  1:103611/1‑150 (MQ=255)
                                                                                                                       cAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTAt                                <  1:1951973/150‑1 (MQ=255)
                                                                                                                        aGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTAtt                               >  1:2170906/1‑150 (MQ=255)
                                                                                                                        aGTCGTTGTGCCTTCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTAtt                               <  2:767210/150‑1 (MQ=255)
                                                                                                                        aGTCGTTGTGCCTTCCAGTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTAtt                               <  2:633022/150‑1 (MQ=255)
                                                                                                                               gtgCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATcgcg                        <  1:1589690/150‑1 (MQ=255)
                                                                                                                                  ccTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGtg                     <  1:986364/150‑2 (MQ=255)
                                                                                                                                   cTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTaa                    >  1:3618517/1‑150 (MQ=255)
                                                                                                                                    ttCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTaaa                   >  1:3082592/1‑150 (MQ=255)
                                                                                                                                    ttCCATTCCTTCATAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTaaa                   >  1:713264/1‑150 (MQ=255)
                                                                                                                                       cATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTg                <  2:816971/150‑1 (MQ=255)
                                                                                                                                           ccTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTc            <  2:4126770/150‑1 (MQ=255)
                                                                                                                                               cATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACa        >  2:223162/1‑150 (MQ=255)
                                                                                                                                               cATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACa        <  1:1436982/150‑1 (MQ=255)
                                                                                                                                                aTAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACac       >  1:3528201/1‑149 (MQ=255)
                                                                                                                                                aTAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACAt       >  2:4185455/1‑150 (MQ=255)
                                                                                                                                                 tAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATa      >  1:1163225/1‑150 (MQ=255)
                                                                                                                                                 tAAAAGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATa      <  2:2300813/150‑1 (MQ=255)
                                                                                                                                                  aaaaGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAg     <  1:2999592/150‑1 (MQ=255)
                                                                                                                                                   aaCGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGc    <  1:2778639/150‑1 (MQ=255)
                                                                                                                                                     cgcgAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCa  >  2:4675333/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCA  >  CP009273/3932578‑3932876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑