Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,966,854 C→A 61.0% R325S (CGT→AGT)  rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,966,8540CA61.0% 201.8 / inf 338R325S (CGT→AGT) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (65/67);  new base A (103/103);  total (168/170)
Fisher's exact test for biased strand distribution p-value = 9.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 342 total aligned reads displayed.

CGAACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACC  >  CP009273/3966705‑3967003
                                                                                                                                                     |                                                                                                                                                     
cGAACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                                                                                                                       >  2:2419373/1‑150 (MQ=255)
  aaCAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGt                                                                                                                                                     >  2:2014972/1‑150 (MQ=255)
   aCAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa                                                                                                                                                    <  2:4151778/150‑1 (MQ=255)
    cAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaa                                                                                                                                                   <  2:1196520/150‑1 (MQ=255)
     aaGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaa                                                                                                                                                  >  2:4354034/1‑150 (MQ=255)
     aaGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaa                                                                                                                                                  <  2:3356915/150‑1 (MQ=255)
        ccGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACg                                                                                                                                               <  1:300699/150‑1 (MQ=255)
        ccGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACg                                                                                                                                               <  1:3118331/150‑1 (MQ=255)
        ccGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACg                                                                                                                                               <  2:3500936/150‑1 (MQ=255)
          gCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGt                                                                                                                                             <  2:1493802/150‑1 (MQ=255)
          gCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGt                                                                                                                                             >  2:4080338/1‑150 (MQ=255)
           cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGtt                                                                                                                                            <  2:2683806/150‑1 (MQ=255)
            aCGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGACTGAGTAAAACGGTTc                                                                                                                                           >  1:4568711/1‑150 (MQ=255)
            aCGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                                                                                                                           >  1:2055271/1‑150 (MQ=255)
              ggCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg                                                                                                                                         >  1:4371233/1‑150 (MQ=255)
              ggCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAg                                                                                                                                         >  1:515584/1‑150 (MQ=255)
               gCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                                                                                                                        <  1:1795107/150‑1 (MQ=255)
               gCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                                                                                                                        <  1:930129/150‑1 (MQ=255)
               gCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                                                                                                                        <  1:2296176/150‑1 (MQ=255)
                 gTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTgg                                                                                                                                      <  2:2453699/150‑1 (MQ=255)
                  tGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt                                                                                                                                     >  1:1066005/1‑150 (MQ=255)
                    gcgcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTAt                                                                                                                                   >  2:3652558/1‑150 (MQ=255)
                     cgcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATc                                                                                                                                  <  2:4540061/150‑1 (MQ=255)
                     cgcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATc                                                                                                                                  >  1:4001103/1‑150 (MQ=255)
                      gcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAATCGGTTCAGTGGTATCt                                                                                                                                 >  1:849986/1‑150 (MQ=255)
                       cACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg                                                                                                                                >  1:3481479/1‑150 (MQ=255)
                         cTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGc                                                                                                                              <  2:1163240/150‑1 (MQ=255)
                          tATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGACTGAGTAAAACGGTTCAGTGGTATCTGGCt                                                                                                                             >  1:2582734/1‑150 (MQ=255)
                          tATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                                                                                                                             <  1:1544927/150‑1 (MQ=255)
                           aTCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTa                                                                                                                            >  1:3967009/1‑150 (MQ=255)
                           aTCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTa                                                                                                                            >  2:3755636/1‑150 (MQ=255)
                             cGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAt                                                                                                                          <  2:600061/150‑1 (MQ=255)
                             cGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAAt                                                                                                                          >  1:2745704/1‑150 (MQ=255)
                                gACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaa                                                                                                                       <  2:3156647/150‑1 (MQ=255)
                                  ccTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAg                                                                                                                     <  1:1015435/150‑1 (MQ=255)
                                   cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                                                                                                                    <  2:3976801/150‑1 (MQ=255)
                                   cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                                                                                                                    >  2:299377/1‑150 (MQ=255)
                                                     gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg                                                                                                  <  1:3877733/150‑1 (MQ=255)
                                                     gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg                                                                                                  >  1:1282857/1‑150 (MQ=255)
                                                        cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAc                                                                                               <  2:1354209/150‑1 (MQ=255)
                                                         gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg                                                                                              <  2:1670964/150‑1 (MQ=255)
                                                         gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg                                                                                              <  1:378574/150‑1 (MQ=255)
                                                            cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCa                                                                                           >  2:2254697/1‑150 (MQ=255)
                                                                  aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATc                                                                                     <  1:2513836/150‑1 (MQ=255)
                                                                  aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATc                                                                                     <  1:613514/150‑1 (MQ=255)
                                                                  aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATc                                                                                     <  2:1422862/150‑1 (MQ=255)
                                                                       cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGc                                                                                <  1:1161255/150‑1 (MQ=255)
                                                                       cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGc                                                                                >  2:1837193/1‑150 (MQ=255)
                                                                          cGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAg                                                                             <  2:301849/150‑1 (MQ=255)
                                                                              aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGtt                                                                         <  1:3577649/150‑1 (MQ=255)
                                                                                gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTa                                                                       <  2:45770/150‑1 (MQ=255)
                                                                                  cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAgg                                                                     >  1:84454/1‑150 (MQ=255)
                                                                                     tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCt                                                                  <  2:661654/150‑1 (MQ=255)
                                                                                     tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCt                                                                  <  2:3878842/150‑1 (MQ=255)
                                                                                       aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGa                                                                >  2:4407430/1‑150 (MQ=255)
                                                                                        tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGaa                                                               <  2:4681923/150‑1 (MQ=255)
                                                                                          cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAg                                                             >  1:1430228/1‑150 (MQ=255)
                                                                                          cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAg                                                             <  2:2302486/150‑1 (MQ=255)
                                                                                               aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTaa                                                        >  1:2460430/1‑150 (MQ=255)
                                                                                                   ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAtttt                                                    <  1:4472543/150‑1 (MQ=255)
                                                                                                    tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTc                                                   >  1:3752789/1‑150 (MQ=255)
                                                                                                       ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGc                                                >  1:321866/1‑150 (MQ=255)
                                                                                                        cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCg                                               >  1:3724738/1‑150 (MQ=255)
                                                                                                          tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                             <  2:494958/150‑1 (MQ=255)
                                                                                                          tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                             >  2:1600424/1‑150 (MQ=255)
                                                                                                             aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGc                                          >  1:3676427/1‑150 (MQ=255)
                                                                                                             aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGc                                          >  1:46056/1‑150 (MQ=255)
                                                                                                             aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGc                                          <  2:1737587/150‑1 (MQ=255)
                                                                                                                  gctggctgCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACa                                     <  2:3291634/150‑1 (MQ=255)
                                                                                                                  gctggctgCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACa                                     <  1:1566148/150‑1 (MQ=255)
                                                                                                                    tggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATg                                   >  2:2487685/1‑150 (MQ=255)
                                                                                                                     ggctgCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGa                                  <  2:2115696/150‑1 (MQ=255)
                                                                                                                      gctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGaa                                 <  1:94783/150‑1 (MQ=255)
                                                                                                                        tgCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAg                               >  1:4143990/1‑150 (MQ=255)
                                                                                                                        tgCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAg                               <  1:1378208/150‑1 (MQ=255)
                                                                                                                          ccGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGt                             <  2:371256/150‑1 (MQ=255)
                                                                                                                           cGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGta                            >  1:2806615/1‑150 (MQ=255)
                                                                                                                           cGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGta                            >  1:4598023/1‑150 (MQ=255)
                                                                                                                           cGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGta                            <  1:4080338/150‑1 (MQ=255)
                                                                                                                           cGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGta                            <  1:1693804/150‑1 (MQ=255)
                                                                                                                             cAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatt                          <  1:3362612/150‑1 (MQ=255)
                                                                                                                             cAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatt                          >  2:841497/1‑150 (MQ=255)
                                                                                                                              agtaaaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTTGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCTGGGCGAGCGTTTATGTCTGAAAGGCTAATTTTCTGCGGAGGCAAACATGAAAGGtatta                         <  1:1749672/147‑1 (MQ=255)
                                                                                                                              aGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatta                         <  1:2241264/150‑1 (MQ=255)
                                                                                                                                gAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATc                       >  1:2606864/1‑150 (MQ=255)
                                                                                                                                   aCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTg                    <  1:1820447/150‑1 (MQ=255)
                                                                                                                                    ccTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg                   <  2:3239015/150‑1 (MQ=255)
                                                                                                                                      tttGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcg                 >  1:1925879/1‑150 (MQ=255)
                                                                                                                                        tGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGACAGGTATTATCCTggcggg               >  2:4213054/1‑150 (MQ=255)
                                                                                                                                        tGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcggg               >  2:541073/1‑150 (MQ=255)
                                                                                                                                         gAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcgggc              >  1:2405121/1‑150 (MQ=255)
                                                                                                                                         gAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGACAGGTATTATCCTggcgggc              >  1:631400/1‑150 (MQ=255)
                                                                                                                                          aaaGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcgggcg             <  2:4285506/150‑1 (MQ=255)
                                                                                                                                           aaGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGgcgggcgg            <  2:880593/150‑1 (MQ=255)
                                                                                                                                             gTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGtt          >  1:4731864/1‑150 (MQ=255)
                                                                                                                                              tGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTc         <  2:4485856/150‑1 (MQ=255)
                                                                                                                                              tGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTc         >  2:2868443/1‑150 (MQ=255)
                                                                                                                                                 aaTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCgg      >  2:3954021/1‑150 (MQ=255)
                                                                                                                                                 aaTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCgg      >  1:1723058/1‑150 (MQ=255)
                                                                                                                                                     cGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCAcc  >  2:4461283/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGAACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACC  >  CP009273/3966705‑3967003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑