Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,076 A→T 100% L114* (TTA→TAA)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,0760AT95.7% 1061.0 / 23.1 287L114* (TTA→TAA) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (9/4);  new base T (167/107);  total (176/111)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 292 total aligned reads displayed.

CATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCA  >  CP009273/3993928‑3994222
                                                                                                                                                    |                                                                                                                                                  
 aTCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTAc                                                                                                                                                  >  2:1396629/1‑150 (MQ=255)
   cAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGc                                                                                                                                                >  1:1391163/1‑150 (MQ=255)
    aaCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGcc                                                                                                                                               >  1:4576884/1‑150 (MQ=255)
         tGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCtt                                                                                                                                          >  1:2896881/1‑150 (MQ=255)
            cccTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctg                                                                                                                                       <  1:3654671/150‑1 (MQ=255)
              cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctgct                                                                                                                                     <  2:2454650/150‑1 (MQ=255)
               tGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctgctg                                                                                                                                    <  2:2349025/150‑1 (MQ=255)
                gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGa                                                                                                                                   >  2:1422035/1‑150 (MQ=255)
                 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                                                                                                                  >  1:589274/1‑150 (MQ=255)
                   tGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCg                                                                                                                                >  2:3006810/1‑150 (MQ=255)
                            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATg                                                                                                                       >  2:3799251/1‑150 (MQ=255)
                             aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACAAAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTATACGCCACCTTCTGCTGACCTTTTTCGATGt                                                                                                                      >  1:372199/1‑150 (MQ=255)
                                gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACa                                                                                                                   <  1:4461286/150‑1 (MQ=255)
                                gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACa                                                                                                                   >  2:630818/1‑150 (MQ=255)
                                 aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                                                                                                                  >  1:3018148/1‑150 (MQ=255)
                                  aTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa                                                                                                                 >  2:2165310/1‑150 (MQ=255)
                                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                                                                                                               >  1:2651413/1‑150 (MQ=255)
                                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                                                                                                               <  2:752367/150‑1 (MQ=255)
                                         tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGTGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                          >  2:1741082/1‑150 (MQ=255)
                                         tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGTGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                          >  2:4126491/1‑150 (MQ=255)
                                         tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                          >  1:1280889/1‑150 (MQ=255)
                                                 ttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAg                                                                                                  <  1:468397/150‑1 (MQ=255)
                                                   gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCt                                                                                                >  2:1552913/1‑150 (MQ=255)
                                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCtt                                                                                               >  2:188440/1‑150 (MQ=255)
                                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCtt                                                                                               <  2:3493655/150‑1 (MQ=255)
                                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCtt                                                                                               <  2:2108009/150‑1 (MQ=255)
                                                     aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCttt                                                                                              <  2:4334538/150‑1 (MQ=255)
                                                      ggCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtc                                                                                             >  2:581920/1‑150 (MQ=255)
                                                        cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTCCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcat                                                                                           <  1:3278152/150‑1 (MQ=255)
                                                           ccAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTcatcat                                                                                        >  1:1018531/1‑150 (MQ=255)
                                                            cAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATa                                                                                       >  1:3383732/1‑150 (MQ=255)
                                                            cAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATa                                                                                       >  2:2221615/1‑150 (MQ=255)
                                                            cAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATa                                                                                       >  2:199793/1‑150 (MQ=255)
                                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAg                                                                                      <  1:3313381/150‑1 (MQ=255)
                                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAg                                                                                      <  2:3816699/150‑1 (MQ=255)
                                                                cgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATg                                                                                   <  1:2048718/150‑1 (MQ=255)
                                                                 gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                  >  2:2586717/1‑150 (MQ=255)
                                                                 gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                  >  1:1660779/1‑150 (MQ=255)
                                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgcc                                                                                 >  2:3339845/1‑150 (MQ=255)
                                                                         ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTc                                                                          <  2:1947087/150‑1 (MQ=255)
                                                                          tgACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                         >  1:3845326/2‑150 (MQ=255)
                                                                          cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                         >  2:2291463/1‑150 (MQ=255)
                                                                          cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                         <  1:3200176/150‑1 (MQ=255)
                                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCAt                                                                        <  2:1925491/150‑1 (MQ=255)
                                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCAt                                                                        >  1:1488119/1‑150 (MQ=255)
                                                                              tttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGc                                                                     >  1:1156333/1‑150 (MQ=255)
                                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGcc                                                                    <  2:774566/150‑1 (MQ=255)
                                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGcc                                                                    >  2:1527141/1‑150 (MQ=255)
                                                                                tATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCa                                                                   >  1:4523576/1‑150 (MQ=255)
                                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                <  2:3104148/150‑1 (MQ=255)
                                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                >  1:2431299/1‑150 (MQ=255)
                                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                >  1:4212201/1‑150 (MQ=255)
                                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                >  1:3939370/1‑150 (MQ=255)
                                                                                       cgcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAc                                                            <  2:100857/150‑1 (MQ=255)
                                                                                       cgcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAc                                                            <  2:100875/150‑1 (MQ=255)
                                                                                       cgcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAc                                                            <  2:3946456/150‑1 (MQ=255)
                                                                                         cgACATAAAACACGCCGCCAGGACGGATAAAGAAAGTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcct                                                          <  2:1044148/150‑1 (MQ=255)
                                                                                          gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                         <  1:2798917/150‑1 (MQ=255)
                                                                                          gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                         <  1:3818733/150‑1 (MQ=255)
                                                                                           aCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctga                                                        <  1:522404/150‑1 (MQ=255)
                                                                                             aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgacc                                                      >  2:1137745/1‑150 (MQ=255)
                                                                                               aaaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccct                                                    <  1:35423/150‑1 (MQ=255)
                                                                                                aaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctg                                                   <  1:3609401/150‑1 (MQ=255)
                                                                                                  acacGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctgac                                                 >  2:1354130/1‑150 (MQ=255)
                                                                                                     cgccgcCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTAt                                              <  2:2799591/150‑1 (MQ=255)
                                                                                                      gccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTAtt                                             <  1:2723120/150‑1 (MQ=255)
                                                                                                       ccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTa                                            <  2:3784767/150‑1 (MQ=255)
                                                                                                       ccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTa                                            >  2:3762178/1‑150 (MQ=255)
                                                                                                         gccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAat                                          >  1:3285181/1‑150 (MQ=255)
                                                                                                                cGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGcc                                   <  2:529289/150‑1 (MQ=255)
                                                                                                                cGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGcc                                   >  2:2736833/1‑150 (MQ=255)
                                                                                                                cGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGcc                                   >  2:3714693/1‑150 (MQ=255)
                                                                                                                cGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGcc                                   <  1:1096332/150‑1 (MQ=255)
                                                                                                                  gATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcag                                 <  1:1396629/150‑1 (MQ=255)
                                                                                                                   aTAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagc                                >  2:1627878/1‑150 (MQ=255)
                                                                                                                   aTAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagc                                <  2:789063/150‑1 (MQ=255)
                                                                                                                   aTAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagc                                <  2:3155420/150‑1 (MQ=255)
                                                                                                                    tAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCCTCTGCACCTGACCCTGACTATTAATATCCGCcagca                               >  2:4023576/1‑150 (MQ=255)
                                                                                                                    tAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagca                               >  1:2265786/1‑150 (MQ=255)
                                                                                                                     aaaGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagcag                              >  1:341840/1‑150 (MQ=255)
                                                                                                                        gAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGc                           >  1:3110378/1‑150 (MQ=255)
                                                                                                                         aaaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCa                          >  1:170934/1‑150 (MQ=255)
                                                                                                                           aTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATg                        <  2:3015769/150‑1 (MQ=255)
                                                                                                                            ttCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGt                       <  2:4250971/150‑1 (MQ=255)
                                                                                                                                cTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACg                   <  2:14858/150‑1 (MQ=255)
                                                                                                                                cTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACg                   <  1:1681677/150‑1 (MQ=255)
                                                                                                                                   cACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTc                >  2:1209389/1‑150 (MQ=255)
                                                                                                                                    aCCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcc               <  2:4503490/150‑1 (MQ=255)
                                                                                                                                    aCCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcc               >  1:2035291/1‑150 (MQ=255)
                                                                                                                                     ccTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTccc              <  2:1646587/150‑1 (MQ=255)
                                                                                                                                       tGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCa            >  1:315504/1‑150 (MQ=255)
                                                                                                                                       tGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCa            >  1:706837/1‑150 (MQ=255)
                                                                                                                                        gAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCAc           >  1:2864898/1‑150 (MQ=255)
                                                                                                                                        gAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCAc           <  1:4668605/150‑1 (MQ=255)
                                                                                                                                          aGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGc         >  1:1958324/1‑150 (MQ=255)
                                                                                                                                            cGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCtt       >  1:1747860/1‑150 (MQ=255)
                                                                                                                                            cGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCtt       >  1:4013166/1‑150 (MQ=255)
                                                                                                                                              agaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCg     <  1:2045266/150‑1 (MQ=255)
                                                                                                                                              agaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCg     >  2:4660077/1‑150 (MQ=255)
                                                                                                                                              agaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCg     >  1:846145/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCA  >  CP009273/3993928‑3994222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑