BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,375,503	T→A	6.4%	I98I (ATT→ATA)	yjeN →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,375,503	0	T	A	6.4%	626.6 / 2.5	192	I98I (ATT→ATA)	yjeN	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (129/48); new base A (2/12); total (131/61)
Fisher's exact test for biased strand distribution p-value = 2.08e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTG  >  CP009273/4375354‑4375652

ggTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAAtt                                                                                                                                                       >  1:3444908/1‑150 (MQ=255)

 gTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTa                                                                                                                                                      <  1:2150806/150‑1 (MQ=255)

  tAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTaa                                                                                                                                                     <  2:478822/150‑1 (MQ=255)

    gCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                                                                                   <  1:769690/150‑1 (MQ=255)

       ccATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAc                                                                                                                                                <  1:724398/150‑1 (MQ=255)

       ccATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAc                                                                                                                                                <  1:1030107/150‑1 (MQ=255)

       ccATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAc                                                                                                                                                >  1:3113096/1‑150 (MQ=255)

        cATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAcc                                                                                                                                               >  1:3086871/1‑150 (MQ=255)

         aTTACCACCCTCAAAGATCGCGATGTTAAACGTTGGGGAAACCGGCCGTATATTTATTGCGCTTATCTTATCGTATGTCTGGTTTTTCGGGGGTATTTAGTGGGATACGGCGATTTGTTTCGTTTGCGATATAGCGAAATTAAGCGACCg                                                                                                                                              <  2:228425/150‑1 (MQ=255)

         aTTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCg                                                                                                                                              >  1:4006574/1‑150 (MQ=255)

          ttAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCgg                                                                                                                                             >  2:86667/1‑150 (MQ=255)

           tAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                                                                                            >  2:2479045/1‑150 (MQ=255)

             acacCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTa                                                                                                                                          >  1:3505199/1‑150 (MQ=255)

                 ccTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCtt                                                                                                                                      >  1:2401050/1‑150 (MQ=255)

                   tCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTAt                                                                                                                                    >  2:4022300/1‑150 (MQ=255)

                   tCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTAt                                                                                                                                    >  1:1044467/1‑150 (MQ=255)

                     acaGATCGGGATGTTAACGGGGGGTTAAACCTGCCTTATTTTTTTTGCGCTTATCTTATCCTATTTCTGTTTTTTCGGGGGTATTTTGTGGGATACGGCTTTTTTTTTCGTTTGGGCTATAGCTAAATAAAGCGACCGGTTACCTTATGa                                                                                                                                  <  2:4687276/148‑1 (MQ=255)

                      aaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAg                                                                                                                                 >  2:1883936/1‑150 (MQ=255)

                       aGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGACTTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGt                                                                                                                                >  2:4485014/1‑150 (MQ=255)

                           cgcgATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTgcgc                                                                                                                            >  1:1327376/1‑150 (MQ=255)

                              gATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCa                                                                                                                         >  2:769175/1‑150 (MQ=255)

                                tGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATg                                                                                                                       >  2:1334718/1‑150 (MQ=255)

                                 ggTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                                                                                      >  2:1102682/1‑150 (MQ=255)

                                    aaaCGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTg                                                                                                                   >  1:2310775/1‑150 (MQ=255)

                                      aCGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTc                                                                                                                 >  1:1671751/1‑150 (MQ=255)

                                          tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAt                                                                                                             >  1:2511667/1‑150 (MQ=255)

                                           ggggAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAtg                                                                                                            >  1:774160/1‑150 (MQ=255)

                                             gtaaaCCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAtgct                                                                                                          >  1:984234/3‑150 (MQ=255)

                                              taaaCCTGCCTGATTTTTTTGGCGCTGTTATTCTCAGATGTCTGGTTTTGCGTGTGTATTTTGGGGTTTACGGGTTTTTTTTTGGTTTGCGTTTTAGCGAATTAAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAtgctg                                                                                                         <  2:4166896/149‑1 (MQ=255)

                                               aaaCCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAtgctgc                                                                                                        >  1:2642968/1‑150 (MQ=255)

                                                aaCCTGCCGGATGTTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGATATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCa                                                                                                       >  1:574174/1‑150 (MQ=255)

                                                aaCCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCa                                                                                                       >  1:574214/1‑150 (MQ=255)

                                                   cTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCt                                                                                                    >  1:1863130/1‑150 (MQ=255)

                                                      ccGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGt                                                                                                 >  1:3389390/1‑150 (MQ=255)

                                                       cgtatatTTTTTGCTATTATATTATCCGATGTCTGTTTTTGCGCGGGTATTTTGTGGTATACGGCTATTTGTTTCTTATGCGCTATAGCTAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGtt                                                                                                <  2:1043940/147‑1 (MQ=255)

                                                       cGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGtt                                                                                                >  2:1263300/1‑150 (MQ=255)

                                                          atatTTATGGCGCTTATCTTAACCGATGTCTGTTTTTTGGGGGGTATTTAGTGTGATACGGAGATTTTTTTCTTATGCTTTATAGCTAATTTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTAt                                                                                             <  2:224577/150‑1 (MQ=255)

                                                           ttttttttGCGTTTTTTTTATCCGTTGTGTGTTTTTTGGGGGGTTTTTTGGGGGATTGGTGTTTTTTTTTGGTTTGGGATTTAGCTAAATTACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTAtt                                                                                            <  1:1113153/148‑1 (MQ=255)

                                                           tatttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTAtt                                                                                            >  1:1409633/1‑150 (MQ=255)

                                                             ttttttGCTCTTTTTTTATCCGTTGTGTTTTTTTTGGGGGGTTTTTTGTGGTATCGGGTTTTTTGTTTTGTTTGGTCAATAGATAAATAACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATtgt                                                                                          <  1:508943/150‑1 (MQ=255)

                                                                 tggcgcTTATCTTACCCGTTGTGTGTTTTTTCGCGGGGAATTTGTGGTATCCGGCGTTTTGTTTCGTAGGCGCTATAGCCAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCg                                                                                      <  2:1428377/148‑1 (MQ=255)

                                                                 gtGCGTTTATCTTATCCGTTGTGTGTTTTTTCGCGGGGATTTTGTGGGATAGGGCTTTTTGTTTCGTATGCGCTATAGCTAATTTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCg                                                                                      <  2:1428380/149‑1 (MQ=255)

                                                                     gcTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTcc                                                                                  >  2:2746912/1‑150 (MQ=255)

                                                                      cttatcttatCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCct                                                                                 >  2:3574599/1‑150 (MQ=255)

                                                                      cttatcttatCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCct                                                                                 >  2:3260966/1‑150 (MQ=255)

                                                                       ttatcttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctc                                                                                >  1:4657177/1‑150 (MQ=255)

                                                                       ttatcttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctc                                                                                >  1:3465692/1‑150 (MQ=255)

                                                                       ttatcttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctc                                                                                >  1:3865402/1‑150 (MQ=255)

                                                                       atatcttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATCCGGCGATTTGTTTAGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCCTGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctc                                                                                >  2:2588695/2‑150 (MQ=255)

                                                                         atcttatcCGTTGTCTGTTTTTTCGCGGGGAATTTGTGGGATACGGCTATTTTTTTCGTTTGCGCTATAGCTAATTTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTg                                                                              <  2:3565737/150‑1 (MQ=255)

                                                                         atcttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTg                                                                              >  2:1530781/1‑150 (MQ=255)

                                                                           cttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGa                                                                            >  2:917497/1‑150 (MQ=255)

                                                                           cttatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGa                                                                            >  2:3185965/1‑150 (MQ=255)

                                                                              atcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATaa                                                                         >  1:3624261/1‑150 (MQ=255)

                                                                               tcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAAc                                                                        >  2:2182922/1‑150 (MQ=255)

                                                                                 cGATGTGTGTTTTTTCGGGGGTATTTTGTGGGATCCGGGTTTTTGTTTCGTATGCGATATTGCTAAATAAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCa                                                                      <  2:2616347/150‑1 (MQ=255)

                                                                                 cGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTTTCTTATGCTGCATCTGGTTTATTGTGTCGTTCCTCTGGATAAACa                                                                      >  1:1983570/1‑150 (MQ=255)

                                                                                 cGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCa                                                                      >  2:3893903/1‑150 (MQ=255)

                                                                                   aTGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATc                                                                    >  1:389947/1‑150 (MQ=255)

                                                                                   aTGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATc                                                                    >  2:2311267/1‑150 (MQ=255)

                                                                                         ggTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTcc                                                              >  1:1901225/1‑150 (MQ=255)

                                                                                                  cgGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGAc                                                     >  2:345688/1‑150 (MQ=255)

                                                                                                   ggggTATTTGTGGGATCCGGCGTTTTGTTTCGTTGGCGCTATCGCGAAATTACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACa                                                    <  2:3444908/150‑1 (MQ=255)

                                                                                                       aaTTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAaga                                                >  2:2970748/1‑150 (MQ=255)

                                                                                                       aaTTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAaga                                                >  2:597898/1‑150 (MQ=255)

                                                                                                        aTTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAagag                                               >  2:1503156/1‑150 (MQ=255)

                                                                                                        aTTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAagag                                               >  1:2245355/1‑150 (MQ=255)

                                                                                                         ttAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGt                                              >  2:1698352/1‑150 (MQ=255)

                                                                                                           tttGGGATAGGGCTTTTTTTTTCGTATGCTCTATAGCAAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTgg                                            <  2:2998979/148‑1 (MQ=255)

                                                                                                           aGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTgg                                            >  2:275964/1‑150 (MQ=255)

                                                                                                            gTGGGATCCGGCGTTTTGTTGCGTTTGCGCTATCGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGga                                           <  2:4282626/150‑1 (MQ=255)

                                                                                                             tGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGAAAAAGAGTGgat                                          >  2:2910553/1‑150 (MQ=255)

                                                                                                             ggggATAGGGCGTTTTGTTTCGTTGGCGATATAGCTAATTTACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGANAAAGAGTGgat                                          <  2:170063/149‑1 (MQ=255)

                                                                                                                    cGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGAtgattgat                                   >  2:3794251/1‑150 (MQ=255)

                                                                                                                    cGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGAtgattgat                                   >  2:842626/1‑150 (MQ=255)

                                                                                                                       cGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGa                                >  1:2047895/1‑150 (MQ=255)

                                                                                                                        gATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGAc                               >  2:1833345/1‑150 (MQ=255)

                                                                                                                         aTTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGAcg                              >  2:2526801/1‑150 (MQ=255)

                                                                                                                          ttttttTCGTATGCGATATTGCTAAATAAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGAcgc                             <  2:1079682/150‑1 (MQ=255)

                                                                                                                             gTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCggg                          >  2:4319728/1‑150 (MQ=255)

                                                                                                                                  ttATGCGTTATAGCTAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTaa                     <  2:2971526/149‑1 (MQ=255)

                                                                                                                                  gTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTaa                     >  2:1615293/1‑150 (MQ=255)

                                                                                                                                   tATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTaac                    >  1:1775753/1‑150 (MQ=255)

                                                                                                                                   tATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTaac                    >  2:3531748/1‑150 (MQ=255)

                                                                                                                                   tATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTaac                    >  1:42548/1‑150 (MQ=255)

                                                                                                                                    tggggcTATCGCTAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTaaca                   <  2:3624261/146‑1 (MQ=255)

                                                                                                                                         cTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTc              <  2:1916103/150‑1 (MQ=255)

                                                                                                                                         cTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTc              >  1:451039/1‑150 (MQ=255)

                                                                                                                                           ataGCTAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTg            <  1:3566258/150‑1 (MQ=255)

                                                                                                                                           ataGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTg            >  2:1670570/1‑150 (MQ=255)

                                                                                                                                            taGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGc           >  1:4238192/1‑150 (MQ=255)

                                                                                                                                             aGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCg          >  2:2568207/1‑150 (MQ=255)

                                                                                                                                             aGCGAAATAAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCg          >  1:218510/1‑150 (MQ=255)

                                                                                                                                               cGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAATAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGAt        >  1:4716657/1‑150 (MQ=255)

                                                                                                                                               cGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGAt        >  1:4633906/1‑150 (MQ=255)

                                                                                                                                                taaaTAACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGAtg       <  2:2401050/149‑1 (MQ=255)

                                                                                                                                                 aatttAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGAtgt      <  1:4622158/147‑1 (MQ=255)

                                                                                                                                                 aaaTAACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGAtgt      <  1:3210879/150‑1 (MQ=255)

                                                                                                                                                     tAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTg  <  2:667317/150‑1 (MQ=255)

                                                                                                                                                     tAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTg  >  2:1010129/1‑150 (MQ=255)

GGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTG  >  CP009273/4375354‑4375652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`