Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,388,902 G→T 100% A558A (GCG→GCT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,388,9020GT97.1% 1090.8 / 17.9 306A558A (GCG→GCT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base T (173/124);  total (178/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 306 total aligned reads displayed.

ATTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCC  >  CP009273/4388755‑4389048
                                                                                                                                                   |                                                                                                                                                  
aTTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCa                                                                                                                                                  <  1:1026498/150‑1 (MQ=255)
aTTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCa                                                                                                                                                  >  2:3157116/1‑150 (MQ=255)
aTTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCa                                                                                                                                                  >  2:274357/1‑150 (MQ=255)
  tGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGt                                                                                                                                                >  1:106426/1‑150 (MQ=255)
    ggTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGGATTCGAACCTGGCAATATTGCTCAGTgg                                                                                                                                              >  2:4590120/1‑150 (MQ=255)
     gTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGa                                                                                                                                             >  2:2343349/1‑150 (MQ=255)
              tCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGaa                                                                                                                                    <  2:1972564/150‑1 (MQ=255)
              tCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGaa                                                                                                                                    >  1:4710197/1‑150 (MQ=255)
               ccAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCATGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGaaa                                                                                                                                   >  1:1407691/1‑150 (MQ=255)
                cAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAAt                                                                                                                                  <  1:2191745/150‑1 (MQ=255)
                 aGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTCTTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                                 >  2:1577911/1‑150 (MQ=255)
                 aGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                                 >  2:2257132/1‑150 (MQ=255)
                  gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCt                                                                                                                                >  1:4133561/1‑150 (MQ=255)
                   tCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtg                                                                                                                               >  1:89873/1‑150 (MQ=255)
                   tCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtg                                                                                                                               <  1:4254549/150‑1 (MQ=255)
                   tCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtg                                                                                                                               <  1:378126/150‑1 (MQ=255)
                     aGATGCACAGCATGTGACCATCAGGGCCGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCGTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATCTTGCGCAGTGGATTGCACGAAATCtgat                                                                                                                             >  2:891755/1‑150 (MQ=255)
                        tGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAg                                                                                                                          <  2:2620271/150‑1 (MQ=255)
                         gCACACCAAGTGACCAGCAGGGCAGTGCCTTCCCCCACCCGCCAACACAATGCACAAATCAAGATGCCTGAACAGACAGGCTACCTGGCGCAGCAGTCCGTATTCGAACCTGGCAACATTGCTCAGTGGATTGCACGAAATCTGATGAGc                                                                                                                         <  2:4725211/150‑1 (MQ=255)
                           acaGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGa                                                                                                                       >  2:4143528/1‑150 (MQ=255)
                            caGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGaa                                                                                                                      <  1:1755959/150‑1 (MQ=255)
                            caGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGaa                                                                                                                      <  1:1511600/150‑1 (MQ=255)
                             aGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAAc                                                                                                                     >  1:3224452/1‑150 (MQ=255)
                                   tgACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATgcgc                                                                                                               >  2:3686127/1‑150 (MQ=255)
                                       cATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTg                                                                                                           <  1:1934952/150‑1 (MQ=255)
                                        aTCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTgg                                                                                                          <  1:3840195/150‑1 (MQ=255)
                                        aTCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTgg                                                                                                          >  2:2300959/1‑150 (MQ=255)
                                         tCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGt                                                                                                         >  2:4441814/1‑150 (MQ=255)
                                           aGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCa                                                                                                       <  2:34405/150‑1 (MQ=255)
                                               cAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATCTTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATgg                                                                                                   <  2:2185712/150‑1 (MQ=255)
                                                aGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGc                                                                                                  >  2:1543636/1‑150 (MQ=255)
                                                 gTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGca                                                                                                 >  1:2978963/1‑150 (MQ=255)
                                                       ttACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGcc                                                                                           >  1:3636090/1‑150 (MQ=255)
                                                       ttACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGcc                                                                                           <  1:3045238/150‑1 (MQ=255)
                                                        tACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCa                                                                                          >  2:2030128/1‑150 (MQ=255)
                                                         aCCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCAt                                                                                         <  1:1738024/150‑1 (MQ=255)
                                                         aCCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCAt                                                                                         >  2:1625378/1‑150 (MQ=255)
                                                              tACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAAccc                                                                                    >  2:3268178/1‑150 (MQ=255)
                                                               aCGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCct                                                                                   <  2:573364/150‑1 (MQ=255)
                                                                cGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctg                                                                                  <  1:4157885/150‑1 (MQ=255)
                                                                cGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctg                                                                                  <  2:1334723/150‑1 (MQ=255)
                                                                 gCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgc                                                                                 >  2:1161187/1‑150 (MQ=255)
                                                                   caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                               >  1:1183901/1‑150 (MQ=255)
                                                                   caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                               >  1:2094584/1‑150 (MQ=255)
                                                                   caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                               <  1:435784/150‑1 (MQ=255)
                                                                   caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                               >  1:4477734/1‑150 (MQ=255)
                                                                   caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                               >  1:3979187/1‑150 (MQ=255)
                                                                        aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGa                                                                          <  2:3975506/150‑1 (MQ=255)
                                                                         aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACTTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGAc                                                                         >  2:1099295/1‑150 (MQ=255)
                                                                         aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGAc                                                                         >  2:742854/1‑150 (MQ=255)
                                                                          aTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACg                                                                        <  2:3771924/150‑1 (MQ=255)
                                                                          aTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACg                                                                        <  2:4523655/150‑1 (MQ=255)
                                                                            ttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTg                                                                      >  2:1379288/1‑150 (MQ=255)
                                                                              aCAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGa                                                                    >  2:1909050/1‑150 (MQ=255)
                                                                              aCAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGa                                                                    <  1:3499396/150‑1 (MQ=255)
                                                                                aaaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAAc                                                                  >  1:1988798/1‑150 (MQ=255)
                                                                                      ttaatCCTGAACTGATAGGATAACTGGCGAATCAGTCCGTATTCGAAACTGTCAATATTGCTCAGTGTATTGATCGAAATCTGATGAGCGAACATGCGCAGTGGGCAATGGCACCGGCCATAAACCGGCTGGCGGACGTGGAACGGatat                                                            >  2:2901348/5‑150 (MQ=255)
                                                                                      tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTAt                                                            >  1:4066764/1‑150 (MQ=255)
                                                                                      tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTAt                                                            >  2:529479/1‑150 (MQ=255)
                                                                                          tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTcc                                                        >  2:2942490/1‑150 (MQ=255)
                                                                                          tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTcc                                                        >  1:2656636/1‑150 (MQ=255)
                                                                                          tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTcc                                                        >  2:1219617/1‑150 (MQ=255)
                                                                                           ccTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCg                                                       >  1:1978015/1‑150 (MQ=255)
                                                                                           ccTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCg                                                       >  2:3554913/1‑150 (MQ=255)
                                                                                              gAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCaa                                                    >  2:324484/1‑150 (MQ=255)
                                                                                                  tGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                                >  2:4401419/1‑150 (MQ=255)
                                                                                                   gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCCGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                               >  1:3855115/1‑150 (MQ=255)
                                                                                                   gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                               >  1:4675074/1‑150 (MQ=255)
                                                                                                     tAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGa                                             <  1:2535529/150‑1 (MQ=255)
                                                                                                      aGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaa                                            <  1:611061/150‑1 (MQ=255)
                                                                                                       ggCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaa                                           <  1:684712/150‑1 (MQ=255)
                                                                                                          tACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcg                                        >  1:877585/1‑150 (MQ=255)
                                                                                                          tACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcg                                        >  1:3351485/1‑150 (MQ=255)
                                                                                                          tACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcg                                        >  1:3560957/1‑150 (MQ=255)
                                                                                                            ccTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc                                      <  1:2424383/150‑1 (MQ=255)
                                                                                                             cTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                     >  1:22971/1‑150 (MQ=255)
                                                                                                             cTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                     >  1:272590/1‑150 (MQ=255)
                                                                                                              tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccgc                                    >  2:4250434/1‑150 (MQ=255)
                                                                                                                 cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                 >  1:1546809/1‑150 (MQ=255)
                                                                                                                    aGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGTTTGCACGAAATCTTATGAGCGAACATGCGCTGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtg                              <  1:3113603/150‑1 (MQ=255)
                                                                                                                      cAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                            <  1:4732734/150‑1 (MQ=255)
                                                                                                                         tCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTg                         <  1:3903282/150‑1 (MQ=255)
                                                                                                                           cGTATTCGACCCTGGCAATATGGCTCAGTGGATGGCACGAAATCTGAGGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGtt                       <  1:1332413/150‑1 (MQ=255)
                                                                                                                            gTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTa                      <  2:1456338/150‑1 (MQ=255)
                                                                                                                              aTTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACTTGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTTTGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACa                    <  2:1407691/150‑1 (MQ=255)
                                                                                                                                tCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAAt                  >  1:96642/1‑150 (MQ=255)
                                                                                                                                   aaCCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg               >  2:2693389/1‑150 (MQ=255)
                                                                                                                                   aaCCGGGCAATTTGGCTCCGTGGATTGCACGAAATCTGCTGAGCGACCCTGCGCCGGGGTCACGGGCACAGGCCATACCCCTGCTGGCGGAGGTGGAACGTTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg               <  1:4402283/150‑1 (MQ=255)
                                                                                                                                    aCCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGt              <  1:1514842/150‑1 (MQ=255)
                                                                                                                                     ccTGGCAATATTGCTCAGTGGATTGCACGAAATGTGAGGAGCTAACATGCGATGTGGTCAATGGCACAGGCAATAACCCTGCTGGCGGACTTGTAACGGTTAGGTCCGCAACTTGTTAAAACGCCGCCGGGTGGTCTGTTACAATCTGtt             <  1:168225/150‑1 (MQ=255)
                                                                                                                                       tGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGa           >  2:1873471/1‑150 (MQ=255)
                                                                                                                                           aaTATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTa       <  2:3224394/150‑1 (MQ=255)
                                                                                                                                           aaTATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTa       >  2:1145715/1‑150 (MQ=255)
                                                                                                                                           aaTATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTa       <  2:853411/150‑1 (MQ=255)
                                                                                                                                            atatTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTAc      >  2:3367379/1‑150 (MQ=255)
                                                                                                                                             tatTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa     >  2:3887535/1‑150 (MQ=255)
                                                                                                                                              atTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACAt    <  2:1245399/150‑1 (MQ=255)
                                                                                                                                                tGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATcc  <  2:2973092/150‑1 (MQ=255)
                                                                                                                                                tGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATcc  <  2:4657932/150‑1 (MQ=255)
                                                                                                                                                tGCTCAGGGGATTGCACGAAATCTGAGGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATcc  <  2:2807350/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCC  >  CP009273/4388755‑4389048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑