| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 361,806 | G→A | 100% | intergenic (‑63/+14) | lacI ← / ← mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 361,806 | 0 | G | A | 97.2% | 37009.1 / inf | 10068 | intergenic (‑63/+14) | lacI/mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (138/143); new base A (4728/5040); total (4875/5193) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 10075 total aligned reads displayed.
GTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTC > CP009273/361657‑361955 | gctggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCa < 2:2397900/150‑1 (MQ=255) ctggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt < 1:618618/150‑1 (MQ=255) tggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt > 1:4721229/1‑150 (MQ=255) tggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt > 2:3621514/1‑150 (MQ=255) ggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTc < 2:1929680/150‑1 (MQ=255) gcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCg < 2:4714350/150‑1 (MQ=255) ccTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa < 1:1174393/150‑1 (MQ=255) tGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTCTAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTCTCATGCCATACCGCGAAAGGTTTTGCACCATTCGATg > 1:1803776/1‑150 (MQ=255) gTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt > 1:698923/1‑150 (MQ=255) gTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt < 2:4246031/150‑1 (MQ=255) tCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt < 1:2768232/150‑1 (MQ=255) caccacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc > 1:4320414/1‑150 (MQ=255) ccacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCaa < 2:293190/150‑1 (MQ=255) cACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt < 1:196306‑M2/150‑2 (MQ=255) cACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt < 2:4064386‑M2/150‑2 (MQ=255) cACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt < 2:1470482‑M2/150‑2 (MQ=255) aCGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtt > 1:3997640‑M2/1‑148 (MQ=255) cgGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat < 2:2663099‑M2/150‑6 (MQ=255) gggAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatt < 2:546026‑M2/150‑7 (MQ=255) ggAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatta > 1:2200106‑M2/1‑143 (MQ=255) aaaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaa < 2:908048‑M2/150‑10 (MQ=255) aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag < 2:1378177‑M2/150‑11 (MQ=255) aCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGGTTTGCACCATTCGATGGTGTCACttaattaaagg < 2:1315719‑M2/150‑12 (MQ=255) cGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggc > 1:3424628‑M2/1‑138 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattataggcatca < 2:115607‑M2/150‑17 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatca < 2:3788339‑M2/150‑17 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACAACACTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAATCGATGGTGTCAAttaattaaaggcatca < 2:3704632‑M2/150‑17 (MQ=255) gATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaa < 2:3451679‑M2/150‑19 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaat < 1:1089749‑M2/150‑20 (MQ=255) tAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaata < 1:1728272‑M2/150‑21 (MQ=255) tAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaata > 2:635405‑M2/1‑130 (MQ=255) agagACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaa < 2:167475‑M2/150‑23 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa < 1:1427694‑M2/150‑24 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa < 1:4245604‑M2/150‑24 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa > 1:3657846‑M2/1‑127 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa < 2:2120565‑M2/150‑24 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa < 1:2053492‑M2/150‑24 (MQ=255) tgacACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaac < 2:3959424‑M2/149‑25 (MQ=255) agaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaac > 2:1562985‑M2/1‑126 (MQ=255) gaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacg < 1:2837862‑M2/150‑26 (MQ=255) acacCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacga < 1:3902570‑M2/150‑27 (MQ=255) cacCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgac > 1:3337689‑M2/1‑123 (MQ=255) acCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaa > 2:2834115‑M2/1‑122 (MQ=255) acCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaa < 2:2574382‑M2/150‑29 (MQ=255) cGGCATACTCTGCGACATCGTATAACGTTAGTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCAGGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaagg > 1:975115‑M2/1‑120 (MQ=255) cGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGACAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaagg < 1:2295382‑M2/150‑31 (MQ=255) aTACTCTGCGACATCGTATACCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctca > 2:2307026‑M2/1‑116 (MQ=255) tGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaa > 1:3162962‑M2/1‑110 (MQ=255) tGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaa < 1:3516337‑M2/150‑41 (MQ=255) cGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaag < 1:4573527‑M2/150‑43 (MQ=255) gACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaaga > 1:213015‑M2/1‑107 (MQ=255) aCATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagac > 1:4406045‑M2/1‑106 (MQ=255) aCATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagac < 1:3601418‑M2/150‑45 (MQ=255) cGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactggg > 1:1510377‑M2/1‑102 (MQ=255) gTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggc > 1:374325‑M2/1‑101 (MQ=255) ataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct < 1:12473‑M2/150‑52 (MQ=255) taACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt < 1:520270‑M2/150‑53 (MQ=255) taACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt > 1:2520017‑M2/1‑98 (MQ=255) aCGTTACTGTTTTCACATCCCCAACCCTGTATTTACTCTCTTCCGGGCGCTATCATGCCATACCGCGATAGGTTTTGCACCATTCGTTGGTGTCATttaattaacggcatcaactaaaacgaaaggctcagtcgaaagactgggcctttc > 1:1001739‑M2/1‑96 (MQ=255) cGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg > 2:79302‑M2/1‑95 (MQ=255) cGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctgtcg < 2:2029214‑M2/150‑56 (MQ=255) cGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctgtcg < 2:4627086‑M2/150‑56 (MQ=255) gTTACGGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattacaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt > 2:1838994‑M2/1‑94 (MQ=255) ttACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt > 2:3110387‑M2/1‑93 (MQ=255) tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt < 1:4648128‑M2/150‑59 (MQ=255) aCTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt > 1:1881182‑M2/1‑91 (MQ=255) cTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta < 2:2490384‑M2/150‑61 (MQ=255) gTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct < 2:715922‑M2/150‑64 (MQ=255) tCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgacaggctcagtcgaaagactgggcctttcgttttatctgtt > 2:1238500‑M2/1‑84 (MQ=255) cacaTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCAGACCGCGAATGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg > 2:1062142‑M2/1‑83 (MQ=255) acaTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt < 2:4430556‑M2/150‑69 (MQ=255) caTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttgtatctgttgtg < 2:452153‑M2/150‑70 (MQ=255) aTTCACCACCCTGACTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt > 2:32667‑M2/1‑80 (MQ=255) aTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGtt > 1:2378459/1‑150 (MQ=255) aTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttgtatctgttgttt < 1:4490303‑M2/150‑71 (MQ=255) ttCACCACCCTGAATTGACTCTCTTCCGGGCACTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg < 1:3815338‑M2/150‑72 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc < 1:1329155‑M2/150‑74 (MQ=255) accCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccgttcgttttatctgttgtttgtcggtg < 1:3000734‑M2/150‑78 (MQ=255) gAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatgtgttgtttgtcggtgaacgc < 1:1647379‑M2/150‑83 (MQ=255) gAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttagcggttgtttgtcggtgaacgc < 1:2923763‑M2/150‑83 (MQ=255) gAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGGGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccgttcgtgttatctgtggtttgtcggtgaacgc < 2:3358793‑M2/150‑83 (MQ=255) aaTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct < 2:2209697‑M2/150‑84 (MQ=255) aaTTGACTCTCTGCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgatttatctgttgtttgtcggtgaacgct > 1:3088784‑M2/1‑67 (MQ=255) ttGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttttctgttgtttgtcggtgaacgctct < 1:414947‑M2/150‑86 (MQ=255) aCTCTCTTACGGGCGCTATCATGCCATACCGCGAAAGGGTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcat < 2:353956‑M2/150‑89 (MQ=255) ctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagta < 1:4072403‑M2/150‑94 (MQ=255) tCCGGGCGCTATCATGCCATACCGCGAACGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtagg > 2:3193458‑M2/1‑55 (MQ=255) cgcgcgccacCACGCCATCCCGCGCAAGGTTTGGCACCAGTCGATGGTGACAAttaattaaagccatcaaatcacacgaaaggctcagtcgcaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggac < 1:881381‑M1/147‑98 (MQ=255) cgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaat < 1:3263622‑M2/150‑102 (MQ=255) gcTAACATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaacggcatcaaataaaacgacagtctcagtcgaaagactgggactttcgtatgatctgttgtttgtcggtgaacgctctcctgagtaggacaaatc > 2:276092‑M2/1‑48 (MQ=255) cTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatcc < 1:1577302‑M2/150‑104 (MQ=255) tATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccg < 1:2452010‑M2/150‑105 (MQ=255) aTCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgc < 2:4342496‑M2/150‑106 (MQ=255) tCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttagctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgcc < 1:79170‑M2/150‑107 (MQ=255) cATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg < 2:3023824‑M2/150‑108 (MQ=255) cATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg < 1:2592994‑M2/150‑108 (MQ=255) gCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGc > 2:386262/1‑150 (MQ=255) gCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccc < 2:1758277‑M2/150‑111 (MQ=255) ccATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccct > 2:4141894‑M2/1‑39 (MQ=255) ccGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTc < 1:2689430/150‑1 (MQ=255) | GTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTC > CP009273/361657‑361955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |