Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,516,734 G→T 17.3% F207L (TTC→TTA)  sgcE ← putative epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,516,7340GT17.3% 1057.6 / 161.9 359F207L (TTC→TTA) sgcEputative epimerase
Reads supporting (aligned to +/- strand):  ref base G (142/155);  new base T (26/36);  total (168/191)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 362 total aligned reads displayed.

ACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCG  >  CP009273/4516586‑4516883
                                                                                                                                                    |                                                                                                                                                     
 cgacgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGaa                                                                                                                                                     >  2:3416421/1‑150 (MQ=255)
 cgacgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGaa                                                                                                                                                     >  2:1575089/1‑150 (MQ=255)
    cgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTg                                                                                                                                                  <  2:2984722/150‑1 (MQ=255)
      gACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAg                                                                                                                                                <  1:4625891/150‑1 (MQ=255)
       aCAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGc                                                                                                                                               <  1:782761/150‑1 (MQ=255)
         aGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTa                                                                                                                                             >  1:3126414/1‑150 (MQ=255)
         aGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTa                                                                                                                                             >  2:3097128/1‑150 (MQ=255)
           tCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAg                                                                                                                                           >  1:3826555/1‑150 (MQ=255)
           tCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAg                                                                                                                                           >  2:3311777/1‑150 (MQ=255)
           tCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTCAg                                                                                                                                           >  1:2643063/1‑150 (MQ=255)
           tCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAg                                                                                                                                           >  1:1556473/1‑150 (MQ=255)
              cTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTc                                                                                                                                        <  1:4214174/150‑1 (MQ=255)
               ttCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTcg                                                                                                                                       >  2:1225955/1‑150 (MQ=255)
                   gCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTcgcgcg                                                                                                                                   >  1:2625205/1‑150 (MQ=255)
                    cATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGa                                                                                                                                  >  1:4202072/1‑150 (MQ=255)
                    cATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGa                                                                                                                                  <  1:157930/150‑1 (MQ=255)
                    cATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGa                                                                                                                                  >  1:1625709/1‑150 (MQ=255)
                     aTAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGAt                                                                                                                                 <  1:1063593/150‑1 (MQ=255)
                      tAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATa                                                                                                                                <  2:549293/150‑1 (MQ=255)
                       aGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAg                                                                                                                               >  2:367289/1‑150 (MQ=255)
                        gCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGt                                                                                                                              <  1:425068/150‑1 (MQ=255)
                        gCCAAAAAGCTCCATGACCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGt                                                                                                                              <  1:3291215/150‑1 (MQ=255)
                         ccAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTc                                                                                                                             >  1:3045699/1‑150 (MQ=255)
                          cAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCa                                                                                                                            <  2:174837/150‑1 (MQ=255)
                               aGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGAGACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGa                                                                                                                       <  2:2886600/150‑1 (MQ=255)
                                  tCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAaga                                                                                                                    <  2:315766/150‑1 (MQ=255)
                                     aTGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGaa                                                                                                                 <  2:4171244/150‑1 (MQ=255)
                                     aTGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGaa                                                                                                                 <  2:1410794/150‑1 (MQ=255)
                                     aTGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGaa                                                                                                                 <  2:1785873/150‑1 (MQ=255)
                                      tGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAAc                                                                                                                <  2:2800145/150‑1 (MQ=255)
                                       ggCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACa                                                                                                               >  1:4060826/1‑150 (MQ=255)
                                        gCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAg                                                                                                              <  1:2828505/150‑1 (MQ=255)
                                         ccTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAgg                                                                                                             >  2:4512157/1‑150 (MQ=255)
                                          ctgctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAggg                                                                                                            >  2:1688990/1‑150 (MQ=255)
                                           tgctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgc                                                                                                           <  1:2126111/150‑1 (MQ=255)
                                           tgctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgc                                                                                                           >  1:1212447/1‑150 (MQ=255)
                                           tgctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCGTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgc                                                                                                           <  2:3639740/150‑1 (MQ=255)
                                            gctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgcg                                                                                                          >  2:4359773/1‑150 (MQ=255)
                                             ctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgcgc                                                                                                         >  1:17076/1‑150 (MQ=255)
                                              tgtgtgGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgcgcg                                                                                                        >  2:81189/1‑150 (MQ=255)
                                              tgtgtgGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgcgcg                                                                                                        >  1:1644590/1‑150 (MQ=255)
                                                  tgGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTACGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCa                                                                                                    >  2:4674009/1‑150 (MQ=255)
                                                  tgGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCa                                                                                                    >  1:1813794/1‑150 (MQ=255)
                                                    gagaGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAAt                                                                                                  >  2:120945/1‑150 (MQ=255)
                                                    gagaGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAAt                                                                                                  <  1:1404112/150‑1 (MQ=255)
                                                     agagGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATc                                                                                                 >  1:1566515/1‑150 (MQ=255)
                                                         gTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTCAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCa                                                                                             >  2:6192/1‑150 (MQ=255)
                                                           gACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCata                                                                                           >  1:2722444/1‑150 (MQ=255)
                                                           gACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCata                                                                                           <  1:696586/150‑1 (MQ=255)
                                                              gATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGt                                                                                        >  1:3208507/1‑150 (MQ=255)
                                                               aTGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACGGGGCGCGCCCAATCACCATATGtt                                                                                       >  1:1496460/1‑150 (MQ=255)
                                                                  ttGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTgcg                                                                                    >  2:2425210/1‑150 (MQ=255)
                                                                  ttGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTgcg                                                                                    <  1:3896190/150‑1 (MQ=255)
                                                                     ccACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGccc                                                                                 <  2:3527210/150‑1 (MQ=255)
                                                                      cacaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGcccc                                                                                >  2:3779532/1‑150 (MQ=255)
                                                                       acaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCa                                                                               >  2:2536410/1‑150 (MQ=255)
                                                                       acaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCa                                                                               <  2:2485654/150‑1 (MQ=255)
                                                                       acaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCa                                                                               <  2:2174020/150‑1 (MQ=255)
                                                                       acaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCa                                                                               >  1:1965854/1‑150 (MQ=255)
                                                                        caGGTCGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAg                                                                              <  2:880454/150‑1 (MQ=255)
                                                                          ggTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgct                                                                            <  2:1000420/150‑1 (MQ=255)
                                                                             aGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgctgct                                                                         <  1:2240457/150‑1 (MQ=255)
                                                                                 cagcaTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCa                                                                     <  2:4419173/150‑1 (MQ=255)
                                                                                  agcaTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAg                                                                    >  1:2002266/1‑150 (MQ=255)
                                                                                    caTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGtt                                                                  >  2:781339/1‑150 (MQ=255)
                                                                                       cTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATCTGTTGCGCCCCAGCTGCTGCCAGTTGCt                                                               <  2:3934202/150‑1 (MQ=255)
                                                                                         gTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCtgt                                                             >  1:584955/1‑150 (MQ=255)
                                                                                         gTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCtgt                                                             <  1:3910960/150‑1 (MQ=255)
                                                                                            tGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCa                                                          >  1:4511626/1‑150 (MQ=255)
                                                                                             gATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAg                                                         <  1:3123182/150‑1 (MQ=255)
                                                                                               tACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCt                                                       <  1:105455/150‑1 (MQ=255)
                                                                                               tACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCt                                                       <  1:1129931/150‑1 (MQ=255)
                                                                                                aCGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTg                                                      <  1:1324443/150‑1 (MQ=255)
                                                                                                 cGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGc                                                     <  1:630683/150‑1 (MQ=255)
                                                                                                 cGGTCAGGTCTCTGGTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGc                                                     <  1:848410/150‑1 (MQ=255)
                                                                                                  ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGcc                                                    <  1:3573861/150‑1 (MQ=255)
                                                                                                   gTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCa                                                   >  2:4124523/1‑150 (MQ=255)
                                                                                                      aGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCg                                                >  2:3832928/1‑150 (MQ=255)
                                                                                                       ggTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGt                                               >  1:1005454/1‑150 (MQ=255)
                                                                                                        gTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTa                                              >  2:266453/1‑150 (MQ=255)
                                                                                                        gTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTa                                              >  1:840990/1‑150 (MQ=255)
                                                                                                         tctcTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTaa                                             <  1:3699930/150‑1 (MQ=255)
                                                                                                         tctcTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTaa                                             <  1:3416421/150‑1 (MQ=255)
                                                                                                              gTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCa                                        <  1:4679755/150‑1 (MQ=255)
                                                                                                                tGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCAcc                                      <  2:2000952/150‑1 (MQ=255)
                                                                                                                     catcaGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCgg                                 <  1:254565/150‑1 (MQ=255)
                                                                                                                      atcaGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGc                                <  2:4118218/150‑1 (MQ=255)
                                                                                                                        caGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGccc                              <  1:4691125/150‑1 (MQ=255)
                                                                                                                             aCAAACTCCTCTAAAAGGGTGGCGAAATGATCTAAGGACTCGCGATAGTCAGATGAAGAGAACAGTGCTCCACCAATCACCAAAAGTTGCGACCCAGCTCCTGCCAGTTGCAGTGCAGCTGACAGCGTAATTCCACCGTCGGCCAAGCAc                         >  2:2098914/1‑150 (MQ=255)
                                                                                                                                    cctcTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCt                  <  1:460651/150‑1 (MQ=255)
                                                                                                                                     ctctTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                 >  1:2054076/1‑150 (MQ=255)
                                                                                                                                         tACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGa             >  1:2903868/1‑150 (MQ=255)
                                                                                                                                          aCAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGaa            <  1:367289/150‑1 (MQ=255)
                                                                                                                                            aGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGaaaa          <  2:4608383/150‑1 (MQ=255)
                                                                                                                                               gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGcc       >  1:2603250/1‑150 (MQ=255)
                                                                                                                                               gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGcc       <  1:2335566/150‑1 (MQ=255)
                                                                                                                                                tGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATCTGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCg      >  2:3179949/1‑150 (MQ=255)
                                                                                                                                                 ggCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGt     <  2:666864/150‑1 (MQ=255)
                                                                                                                                                  gCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTg    <  1:3246005/150‑1 (MQ=255)
                                                                                                                                                    gAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCg  >  2:1900108/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCG  >  CP009273/4516586‑4516883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑