BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,596,127	G→T	5.7%	intergenic (‑24/+5)	leuP ← / ← leuQ	tRNA‑Leu/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,596,127	0	G	T	5.7%	724.3 / 2.5	226	intergenic (‑24/+5)	leuP/leuQ	tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (146/56); new base T (3/20); total (149/77)
Fisher's exact test for biased strand distribution p-value = 4.09e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

CGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAG  >  CP009273/4595980‑4596276

 gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttt                                                                                                                                                     <  1:4635251/150‑1 (MQ=255)

  cacacgcATCTTACTTTTTTTGATGTCGCGTGGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGACCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                                                                                                                                                    <  2:2569264/144‑1 (MQ=21)

  cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAAAGTGGtttt                                                                                                                                                    >  2:2717191/1‑150 (MQ=255)

    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                                                                                                                                                  <  1:1076114/150‑1 (MQ=255)

    aaGTCTTCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGTGGGGGGGGACTTGAACCCCCAAGTCCGTAATAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCTAGCAATATCAACGTGGTTTTTg                                                                                                                                                  <  1:3463448/149‑1 (MQ=21)

     aGTCATCTTACTTTTTTTGATCTCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                                                                                                                 <  2:1020743/150‑1 (MQ=255)

     aGTAATCTTACTTTTTTTGATATCGCCTCGTTGGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                                                                                                                 <  1:3723120/150‑1 (MQ=255)

      gTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                                                                                                                                <  2:2641957/150‑1 (MQ=255)

        cATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                                                                                                                                              <  1:2656513/150‑1 (MQ=255)

            ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg                                                                                                                                         >  2:518365/1‑150 (MQ=255)

               cTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                                                                                                      >  1:3192384/1‑150 (MQ=255)

               cTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                                                                                                      >  1:2811546/1‑150 (MQ=255)

                 ttttttTATTTCTCCTCGTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggggg                                                                                                                                    <  1:3089422/150‑1 (MQ=255)

                 ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggggg                                                                                                                                    >  1:1029023/1‑150 (MQ=255)

                   ttttGATCTCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACCCCTGAAGCGAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGa                                                                                                                                  <  2:4731436/150‑1 (MQ=255)

                   ttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACAGCCAGCACTATCAAAGTGGTTATAGCTGCGA‑ggggggggc                                                                                                                                  >  2:4508634/1‑149 (MQ=18)

                     tagATACCCCCTCGTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCTAAAGAACACAAACCCCTGAAGCTAGCGCGTCGACCAAGTCCGCCACCTACGCCCAGAGCTAGCAAGCTCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACt                                                                                                                                <  2:1155069/148‑1 (MQ=18)

                       tatatCGCCTCTTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTg                                                                                                                              <  2:58891/149‑1 (MQ=255)

                              ccTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTGGTTGTTGGTGCGA‑GGGGGGGGCCTTGAAccccc                                                                                                                       >  2:368256/1‑150 (MQ=255)

                               cTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTGCGA‑GGGGGGTGACTTGAACCCCCa                                                                                                                      >  1:2037164/1‑150 (MQ=255)

                                tCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCAc                                                                                                                     >  1:2906481/1‑150 (MQ=255)

                                 cgattGGTGCGAGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTTCCTCCTGAATCAATCGCGTCTACAAATTCGGACTTCGTCGCACTCTTCTAGAAAGCTAAACGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                                                                                                                    <  1:3928565/147‑1 (MQ=12)

                                  gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGt                                                                                                                   >  1:119494/1‑150 (MQ=255)

                                  gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGt                                                                                                                   >  1:3070985/1‑150 (MQ=255)

                                   tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCACACGTc                                                                                                                  >  1:158161/1‑150 (MQ=255)

                                    tgggTGCGCGGGGGGGGACTTGACCCCCCACGTCCGTTTTAACACTATCTCCTGATGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCTAGAATTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTcc                                                                                                                 <  1:688523/148‑1 (MQ=21)

                                      ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGTTTATTGGAGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGt                                                                                                               >  2:2314768/1‑150 (MQ=255)

                                       gcgcgcGGGGGGGGCCTCGAACCCCCCCGCCCGAACGCACACTCACACCTGTAGCTTGCGCGTCTACCAATCCAGACCCATACGCCCTCTGCGAGCATTTTCACCTTGGTTTCTGTGGCGA‑GTTGGGGGACTTGAACCCCCACGTCCGTa                                                                                                              <  2:4742096/148‑1 (MQ=12)

                                       gTGCGTGGGGGGGGACTTGCACCCCCACGTCCGTAATAAAACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATATTCGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                              <  2:4263805/150‑1 (MQ=21)

                                       gTGCGTGGGGGGGGACTTGAACCCCCACGTCCTTAAGAACACTAACACCTTAAGCTAGCGCGTATACCAATTCCGCCACCTTCGCCCATTGCTAGAAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                              <  2:2245739/150‑1 (MQ=25)

                                       gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                              >  1:4153809/1‑150 (MQ=255)

                                          tgtgggggggtAGTGGAACCCCCACTTCTGTATGAACACTAACACCTGATGCTCGCTCGTCTACCATTTCCCCCACTTTCGCCCAGTGCGTGCAATATAAACGTGTTTTTTGGTGGGC‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                           <  2:3577744/147‑1 (MQ=21)

                                          ggtggggggggACTTGACCCCCCACGTCGGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCTGTGCGAGAAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                           <  2:2151842/147‑1 (MQ=25)

                                          ggtggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTCCCGCGTCTACCAATTCCGCCACCTTCGCCCAGTTCTAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                           <  2:1446788/147‑1 (MQ=25)

                                          cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTGGTTGTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTCAgg                                                                                                           >  2:4025196/1‑150 (MQ=255)

                                            tggggggggATTTGACCCCCCTCGTCCTTAAGAACACTAACACCTTAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCCTTGCGTTATATATAAACGTGGTTTTTGGTGGGT‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                                         <  1:3612291/149‑1 (MQ=21)

                                              gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                                                                       >  2:3507524/1‑150 (MQ=255)

                                                 ggggcgttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTGTTGGTGCGA‑GGGGGGGGTCTTGAACCCCCACGTCCGTGAGGACACTaa                                                                                                    >  2:3399465/7‑150 (MQ=25)

                                                 ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCACACGTCCGTAAGGACACTaa                                                                                                    >  2:3736492/1‑150 (MQ=255)

                                                  gtgtcTTGAACCCCCACGTCCTTAAGAACACTAACACCTGAAGATAGCTCTTCTACACTTTCCGCCAACTTCGCCATGTGCGAGAAATATAAACGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                                                                                   <  2:2726243/146‑1 (MQ=25)

                                                   ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca                                                                                                  >  2:377952/1‑150 (MQ=255)

                                                    gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                                 >  1:3746491/1‑150 (MQ=255)

                                                    gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAAACCCCACGTCCGAAAGGACACTAacac                                                                                                 >  2:3844412/1‑150 (MQ=255)

                                                        tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTCGTTATTGGAGCGA‑GGGGCGGGACTTGAACCCCCACGTCCGTAAGGACCCTAACACCTGa                                                                                             >  2:858224/1‑150 (MQ=25)

                                                        gcaaCCCCCCCGTCCGTAAGACCACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTGCGTGCTATTACATCGTGGTTTTTGGTGCGT‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                                                             <  2:1646457/148‑1 (MQ=21)

                                                         gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                                                                            >  2:1182160/1‑150 (MQ=255)

                                                          aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                           <  1:1486303/150‑1 (MQ=255)

                                                          aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                           >  1:245519/1‑150 (MQ=255)

                                                             ccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                        >  1:3066252/1‑150 (MQ=255)

                                                              cccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                       >  2:1300951/1‑150 (MQ=255)

                                                               ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGGAAGGACACTAACACCTGAAGCTAgc                                                                                      >  1:1276560/1‑150 (MQ=255)

                                                               ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCACTATCAACGTGGTGGTGGGAGCGA‑GGCGCGGGCCAGGAACCCCCACGTCCGGAAGGACACTCACACCTGAAGCTAgc                                                                                      >  1:445181/1‑150 (MQ=21)

                                                                cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                     >  1:3792771/1‑150 (MQ=255)

                                                                cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                     >  1:236950/1‑150 (MQ=255)

                                                                    tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                                 >  2:759282/1‑150 (MQ=255)

                                                                    tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                                 >  2:3127390/1‑150 (MQ=255)

                                                                        tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGCCTTGAACCCCCACGTCCGGAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                             >  2:2516409/1‑150 (MQ=255)

                                                                         aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                            >  1:2750159/1‑150 (MQ=255)

                                                                            aaCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                                         >  1:1078561/1‑150 (MQ=255)

                                                                              cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                       >  1:2396463/1‑150 (MQ=255)

                                                                              cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTGTAGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCTAAAGGACACTAACACCTGAAGCTAGCGCGACTACCAATTcc                                                                       >  2:2822558/1‑150 (MQ=25)

                                                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                      >  2:4710302/1‑150 (MQ=255)

                                                                                 tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                    >  2:450908/1‑150 (MQ=255)

                                                                                  aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTCACACCTGTAGCTAGCGCGTCTACCAATTCCGccc                                                                   >  1:2355112/1‑149 (MQ=255)

                                                                                  aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                   >  1:4166993/1‑150 (MQ=255)

                                                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                               >  2:4328521/1‑150 (MQ=255)

                                                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                               >  1:3711724/1‑150 (MQ=255)

                                                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGGAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                               >  1:3577476/1‑150 (MQ=255)

                                                                                       cTGAAGCTCGCGCGTAAACAACTCCCGCCACCTACGCCCATACCTAGCAATCTAAACGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                              <  2:949953/150‑1 (MQ=14)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                                                        >  1:4186529/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAcc                                                       >  2:833810/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAcc                                                       >  2:1741674/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAcc                                                       >  2:1741700/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCa                                                      >  2:4553276/1‑150 (MQ=255)

                                                                                                 cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCAACCGTCGCATACCATc                                                    >  1:2153009/1‑150 (MQ=255)

                                                                                                   cgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                                  >  1:799118/1‑150 (MQ=255)

                                                                                                     tCTACCAATTCCGCCACCTTCGCCCTGTGCTAGAAATATATACTTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt                                                <  1:2280316/150‑1 (MQ=18)

                                                                                                       tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt                                              >  1:3060997/1‑150 (MQ=255)

                                                                                                          cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                           >  2:1582443/1‑150 (MQ=255)

                                                                                                            aTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                         >  2:3508681/1‑150 (MQ=255)

                                                                                                            aTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                         <  2:4153809/150‑1 (MQ=255)

                                                                                                               ccGCCTCCTTCGCACCGTGCGAGATTTTTTATCGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                                      <  2:3201162/150‑1 (MQ=17)

                                                                                                               ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGT‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                                      <  1:995349/150‑1 (MQ=255)

                                                                                                                cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                                     >  1:466693/1‑150 (MQ=255)

                                                                                                                   cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTg                                  >  2:802664/1‑150 (MQ=255)

                                                                                                                    aCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGc                                 >  2:3172959/1‑150 (MQ=255)

                                                                                                                      cTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGTGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                               >  2:2451211/1‑150 (MQ=255)

                                                                                                                        tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc                             >  2:2886424/1‑150 (MQ=255)

                                                                                                                        tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc                             <  2:2638132/150‑1 (MQ=255)

                                                                                                                          gCCCAGTGCTAGAAATATCAACGTGTTTTTTGGTGCGT‑GGGGGGGTAATTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc                           <  1:518365/150‑1 (MQ=21)

                                                                                                                             cAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGa                        >  1:1022220/1‑150 (MQ=255)

                                                                                                                               gTGCGATCATTATCTACGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                      <  2:4272731/150‑1 (MQ=255)

                                                                                                                               gTGCGAGCAATATCAACGGGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                      <  1:1300951/150‑1 (MQ=255)

                                                                                                                                  cGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcgc                   >  2:150489/1‑150 (MQ=255)

                                                                                                                                   gAGCAATCTCACCGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCa                  <  1:1798193/150‑1 (MQ=255)

                                                                                                                                       actaccAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt              <  2:2037164/145‑1 (MQ=255)

                                                                                                                                          atCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGt           >  2:1055288/1‑150 (MQ=255)

                                                                                                                                              tcGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtt       <  1:3871221/149‑1 (MQ=255)

                                                                                                                                                gTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACAAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACAACGGAGGCGCATTCTAGttttttg     >  2:3294676/1‑144 (MQ=255)

                                                                                                                                                  ggTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                              <  1:2704278/91‑1 (MQ=35)

CGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAG  >  CP009273/4595980‑4596276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`