Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 400,819 A→C 12.5% V94V (GTT→GTG)  proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273400,8190AC12.5% 641.7 / 26.8 265V94V (GTT→GTG) proCpyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (154/71);  new base C (2/38);  total (156/109)
Fisher's exact test for biased strand distribution p-value = 8.57e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAG  >  CP009273/400670‑400968
                                                                                                                                                     |                                                                                                                                                     
gTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGaa                                                                                                                                                       <  2:3725938/150‑1 (MQ=255)
gTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGaa                                                                                                                                                       <  1:1488584/150‑1 (MQ=255)
  aTCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAAc                                                                                                                                                     >  2:2983628/1‑150 (MQ=255)
  aTCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAAc                                                                                                                                                     >  2:2371379/1‑150 (MQ=255)
    cttctGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGACACGa                                                                                                                                                   >  2:2862346/1‑150 (MQ=255)
       ctGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCa                                                                                                                                                >  2:1141718/1‑150 (MQ=255)
        tGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCag                                                                                                                                               >  1:4350765/1‑150 (MQ=255)
        tGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCag                                                                                                                                               >  1:1967659/1‑150 (MQ=255)
            gTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCagagag                                                                                                                                           >  2:1833331/1‑150 (MQ=255)
              tACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTc                                                                                                                                         >  1:2145434/1‑150 (MQ=255)
                cctgcggGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCTGTGCGGGTGTTTTCGGCATGGCGCGTATAATTTTCCGGTCTTGGCCCAGCGCGCGGGAATGCTGGCCGTGCGTGACACCTGCAGAAATACACACGACCAGAGAGTCtt                                                                                                                                       <  2:4424628/147‑1 (MQ=255)
                 cAGCGCGTTTGGGGTTACGGAGGTCATCCCGGCATTAACCTGTGGGGGTGTGTTCGGCATGGCGGGGATAATTTTCCGGTCTTGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCttt                                                                                                                                      <  2:2533777/150‑1 (MQ=255)
                 cAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCttt                                                                                                                                      >  1:2742011/1‑150 (MQ=255)
                      cgTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGTGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCCGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCa                                                                                                                                 <  2:1529917/150‑1 (MQ=255)
                      cgTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCa                                                                                                                                 >  2:1144705/1‑150 (MQ=255)
                       gTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGGGGGTGTGTTCGGCATGGCGGGGATAATTTTCCGGTCCTGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAg                                                                                                                                <  2:4656796/150‑1 (MQ=255)
                       gTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAg                                                                                                                                >  1:367585/1‑150 (MQ=255)
                          tGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCt                                                                                                                             >  1:166336/1‑150 (MQ=255)
                              gTTAGGGAGTTCATCCGGGATTTAACCTGTGGGGGTGTTTTCGGCTGGGCGGGGATATTTTTCCGGTAATGGCCCAGCGCGGGGGCAAGCTGGTCGAGCGTGACACCTGCAGAAATACAAACGACCAGAGAGTCTTTATTCAGGCTGGAg                                                                                                                         <  1:4736392/150‑1 (MQ=255)
                              gTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAg                                                                                                                         >  2:932150/1‑150 (MQ=255)
                               ttACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAgg                                                                                                                        >  1:2645208/1‑150 (MQ=255)
                               ttACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAgg                                                                                                                        >  2:453221/1‑150 (MQ=255)
                               ttACGGAGGACATCCGGGCATTAACCTGGGGGGGTGTGTTCGGCTGGGCGCGGATATTTTTCCGGACATGGCCCAGCGCGCGGGCAAGCGGGGCGAGCGTCACACCTGCAGCAATACCAACGACCAGAGAGTCTTTATTCAGGCTGGAgg                                                                                                                        <  2:873844/150‑1 (MQ=255)
                                 aCGGAGGTCATCCCGGCATTAACCAGTGAGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGTTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTg                                                                                                                      >  1:1515535/1‑150 (MQ=255)
                                     gggTCATCCCGGCATTAACCAGGGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGAAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGAttt                                                                                                                  <  2:3951295/149‑1 (MQ=255)
                                        tCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGc                                                                                                               >  1:2078495/1‑150 (MQ=255)
                                         cATCCCGGCATTAACCCGGGCGGGAGGGTTCGGCTTGGCGCGTATATTTTTCCGGTCTTGGCCCAGCGCGCGGGAACGCTGGTCGAGCGTGACACCTGCAGAAATACAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCt                                                                                                              <  1:4233163/150‑1 (MQ=255)
                                         cATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATATAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCt                                                                                                              <  2:2994628/150‑1 (MQ=255)
                                         cATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCt                                                                                                              >  2:4678810/1‑150 (MQ=255)
                                          aTCCCGGCATTAACCTGTGCGGGAGTGTTCGGCTGGGCGCGGATAATTTTCCGGTCAGGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTa                                                                                                             <  2:563151/150‑1 (MQ=255)
                                            cccGGAATTAACCTGGGCGGTAGTGTTGGGCTTGGCGCGTATAATTTTCCGGTCAGGGCCCAGCGCGCGGGAAAGGTGGTCGAGCGTGACACCTGCAGAAATACACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAg                                                                                                           <  2:4022193/150‑1 (MQ=255)
                                             ccGGAATTAACCCGTGGGGGAGTGTTCGGCAGGGCGCGGATAATTTGCCGGACATGGCCCCGGGCGGGGGCAAGCGGGTCGAGCGTGACCCCTGCAGAAATACACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGc                                                                                                          <  1:558972/150‑1 (MQ=255)
                                              cGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCa                                                                                                         >  1:3019189/1‑150 (MQ=255)
                                               ggCATGACCCCGTGCGGGTGTGGGCGGCCGGGCGCGGATAATTTGGGGGTCGGGGCCCCGCGCGGGGGCACGCGGGGCGCGCGTGACACCTGCAGCAATCGACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCAc                                                                                                        <  2:3968569/150‑1 (MQ=255)
                                                gCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACt                                                                                                       >  2:3562821/1‑150 (MQ=255)
                                                gCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGAACt                                                                                                       >  1:992513/1‑150 (MQ=255)
                                                 cATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACtt                                                                                                      >  2:3504767/1‑150 (MQ=255)
                                                 cATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACtt                                                                                                      >  2:1263302/1‑150 (MQ=255)
                                                 cATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACtt                                                                                                      >  1:2287495/1‑150 (MQ=255)
                                                 cATTAACCAGTGCGGGAGAGTTCGGCATGGCGCGGATAATTTTCAGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGGGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGATGGAGGTGATTTCGCTAAGCACtt                                                                                                      >  1:4113174/1‑150 (MQ=255)
                                                  tttAACCCGTGGGGGAGGGTTCGGCTTGGCGCGTATAATTTTCCGGTCTTGGCCCCGCGCGCGGGCATGCGGGCCGAGCGTCACACCTGCAGCAATAGACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACttt                                                                                                     <  1:2938863/149‑1 (MQ=255)
                                                  aTTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACttt                                                                                                     >  1:4202199/1‑150 (MQ=255)
                                                  aTTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACttt                                                                                                     >  1:2351032/1‑150 (MQ=255)
                                                   ttAACCAGTGCGGGCGTGTTCGGCATGGCGCGTATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATACAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTa                                                                                                    <  1:1154398/150‑1 (MQ=255)
                                                     aaCCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAAt                                                                                                  >  1:2677771/1‑150 (MQ=255)
                                                       cctgTGGGGGTGTTTTCGGCTTGGGGGTGATATTTTCCCGGTACTGCCCCAGCGGGGGGGAATGGGGGTCGCGCTTGACACCTGCACCAATCCACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCa                                                                                                <  1:824541/147‑1 (MQ=255)
                                                        cAGTGGGTGTGTGTTCGGCAGGGGGCGTATAATTTTCCGTTAATGGCCCTGGGCGCGGGCAAGGGGGTCGTGCGTGAAACCTCAAGAAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCat                                                                                               <  1:683377/150‑1 (MQ=255)
                                                        cAGTGCGGGTGTGTTCGGCTTGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCat                                                                                               <  2:1825796/150‑1 (MQ=255)
                                                        cAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGCGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCat                                                                                               <  2:547294/150‑1 (MQ=255)
                                                         tgTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCatg                                                                                              <  2:2140769/149‑1 (MQ=255)
                                                         aGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCatg                                                                                              >  1:2919227/1‑150 (MQ=255)
                                                           ggggggTGTGTTCGGCTTGGGGCGTATTTTTTTCGGGTCTTGGCCCCGCGGGGGGGCATGGTGGTGGTGGGTGACACGTGCCGAAATACACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCatgat                                                                                            <  1:3711334/147‑1 (MQ=255)
                                                             cGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGc                                                                                          >  2:2215233/1‑150 (MQ=255)
                                                                  gtgtTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGt                                                                                     >  1:918777/1‑150 (MQ=255)
                                                                     ttCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTa                                                                                  >  1:7369/1‑150 (MQ=255)
                                                                     ttCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTa                                                                                  >  1:4055567/1‑150 (MQ=255)
                                                                      tCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTaa                                                                                 >  2:2382484/1‑150 (MQ=255)
                                                                       cgtaaTGGCGCGTATATTTTTCCGTTCATGGCCCAGCGCGCGGGCAAGCTGGTCTAGCGTTAAACCTGACGCAATACAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAAc                                                                                <  2:116139/146‑1 (MQ=255)
                                                                        ggCTTGGCGCGTATATTTTTCCGGTCATGGCCCAGCGCGCGGGAACGCTGGTCGAGCGTGACACCTGCAGAAATAGACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACg                                                                               <  1:2557659/150‑1 (MQ=255)
                                                                          cATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGgc                                                                             >  1:1413894/1‑150 (MQ=255)
                                                                           tgggCGGGGTTATTTTTCCGGTCCTGGCCCCGGGCGCGGGCATGGGGGTCGCGGGTGACACCTGCAGCAATCGACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGgca                                                                            <  1:2186207/148‑1 (MQ=255)
                                                                            tGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCACGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGgcag                                                                           <  2:1806966/150‑1 (MQ=255)
                                                                            tGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGgcag                                                                           >  2:3899184/1‑150 (MQ=255)
                                                                                   gATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAat                                                                    >  1:1830505/1‑150 (MQ=255)
                                                                                   gATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAat                                                                    <  2:734626/150‑1 (MQ=255)
                                                                                     tAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAatga                                                                  >  2:1618463/1‑150 (MQ=255)
                                                                                     tAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAatga                                                                  >  2:1616936/1‑150 (MQ=255)
                                                                                      aaTTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAatgat                                                                 >  1:3620562/1‑150 (MQ=255)
                                                                                       aTTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAatgatg                                                                <  2:4733309/150‑1 (MQ=255)
                                                                                        ttttCCGGTCTGGGCCCAGCGCGGGGGAACGGTGGTCGCGCGTGACACCTGCAGAAATACACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGt                                                               <  1:4550637/150‑1 (MQ=255)
                                                                                         tttCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTc                                                              >  1:1551936/1‑150 (MQ=255)
                                                                                         tttCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTc                                                              <  1:1144705/150‑1 (MQ=255)
                                                                                              ggTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGa                                                         >  1:3353839/1‑150 (MQ=255)
                                                                                                 cATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGAttt                                                      <  2:4096100/150‑1 (MQ=255)
                                                                                                  aTGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTg                                                     >  1:2026541/1‑150 (MQ=255)
                                                                                                        cAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCAc                                               >  2:3249475/1‑150 (MQ=255)
                                                                                                         aGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCAct                                              >  2:3010744/1‑150 (MQ=255)
                                                                                                              gcgGGCAAGCGGGTCGAGCGTGACACCGGCAGCAATAGACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTg                                         <  2:2242642/150‑1 (MQ=255)
                                                                                                                        tGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCt                               >  1:465363/1‑150 (MQ=255)
                                                                                                                           tCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTgcg                            >  2:383516/1‑150 (MQ=255)
                                                                                                                            cGAGCGTGACACCTGCAGCAATATAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTgcgg                           <  2:1412894/150‑1 (MQ=255)
                                                                                                                                   gACACCTGCAGCAATACAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGAt                    <  2:810166/150‑1 (MQ=255)
                                                                                                                                    acacCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATg                   >  1:827266/1‑150 (MQ=255)
                                                                                                                                      acCTGCAGCAATATACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGcc                 <  2:3602593/150‑1 (MQ=255)
                                                                                                                                       ccTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCg                >  2:3802218/1‑150 (MQ=255)
                                                                                                                                         tGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGaa              >  2:4157456/1‑150 (MQ=255)
                                                                                                                                          gcagcaATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAAc             >  2:1769194/1‑150 (MQ=255)
                                                                                                                                           cagcaATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACt            >  2:1943250/1‑150 (MQ=255)
                                                                                                                                           cagcaATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACt            >  2:2044708/1‑150 (MQ=255)
                                                                                                                                           cagcaATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACt            >  1:2849263/1‑150 (MQ=255)
                                                                                                                                              caATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGt         >  1:359749/1‑150 (MQ=255)
                                                                                                                                              caATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGt         >  2:279832/1‑150 (MQ=255)
                                                                                                                                               aaTAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTc        >  2:2692224/1‑150 (MQ=255)
                                                                                                                                                 tAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCAt      >  1:3871493/1‑150 (MQ=255)
                                                                                                                                                  aGACACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATg     <  1:3930528/150‑1 (MQ=255)
                                                                                                                                                  aGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATg     >  2:1721587/1‑150 (MQ=255)
                                                                                                                                                  aGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATg     >  1:2700834/1‑150 (MQ=255)
                                                                                                                                                   gAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGc    >  1:3146286/1‑150 (MQ=255)
                                                                                                                                                    aaaCGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCa   >  2:2929011/1‑150 (MQ=255)
                                                                                                                                                     aaCGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAg  >  2:684094/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAG  >  CP009273/400670‑400968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑