Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 411,027 G→T 100% L61L (CTC→CTA)  sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273411,0270GT96.8% 990.3 / 13.8 278L61L (CTC→CTA) sbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/9);  new base T (130/139);  total (130/148)
Fisher's exact test for biased strand distribution p-value = 3.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 288 total aligned reads displayed.

CTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGA  >  CP009273/410880‑411173
                                                                                                                                                   |                                                                                                                                                  
cTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTagatag                                                                                                                                                  >  2:547213/1‑150 (MQ=255)
cTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTagatag                                                                                                                                                  <  1:4159056/150‑1 (MQ=255)
 tGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAgagaga                                                                                                                                                 <  2:3050070/150‑1 (MQ=255)
  gCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAc                                                                                                                                                >  1:3401877/1‑150 (MQ=255)
  gCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAc                                                                                                                                                <  2:3585714/150‑1 (MQ=255)
  gCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAc                                                                                                                                                <  1:3608051/150‑1 (MQ=255)
   cAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcg                                                                                                                                               >  1:3791789/1‑150 (MQ=255)
     aaTTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGAcgcg                                                                                                                                             >  1:903232/1‑150 (MQ=255)
      aTTACCGTCGGGTTGGTTTCGCGCCCGATTCTGGCTCCAGAATGCTCGGTACGCTTCACCTTTCACTTCTAACTCCACCTCCGAAAGTCATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCgg                                                                                                                                            <  1:2597449/150‑1 (MQ=255)
       ttACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGa                                                                                                                                           >  2:4215885/1‑150 (MQ=255)
        tACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAg                                                                                                                                          >  1:67717/1‑150 (MQ=255)
             tCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCg                                                                                                                                     <  1:3029592/150‑1 (MQ=255)
                 gttggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGAt                                                                                                                                 <  1:3581849/150‑1 (MQ=255)
                    ggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCTCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGTTACa                                                                                                                              <  2:1725167/150‑1 (MQ=255)
                    ggttACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACa                                                                                                                              <  2:2864136/150‑1 (MQ=255)
                       tACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAgcg                                                                                                                           >  1:1879976/1‑150 (MQ=255)
                         cgcgCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGcc                                                                                                                         <  2:4281629/150‑1 (MQ=255)
                              ccGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACa                                                                                                                    <  2:756448/150‑1 (MQ=255)
                               cGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaa                                                                                                                   >  1:2286206/1‑150 (MQ=255)
                               cGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGAGATTGTGAAACGTAAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACaa                                                                                                                   >  1:2445248/1‑150 (MQ=255)
                                  ttCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATg                                                                                                                <  1:4454038/150‑1 (MQ=255)
                                    cTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGc                                                                                                              >  2:3650424/1‑150 (MQ=255)
                                       gCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTc                                                                                                           >  2:4662592/1‑150 (MQ=255)
                                       gCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTc                                                                                                           <  2:1858210/150‑1 (MQ=255)
                                        cTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTcc                                                                                                          <  2:1980271/150‑1 (MQ=255)
                                            aGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcagca                                                                                                      <  1:1921992/150‑1 (MQ=255)
                                             gAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCcagcag                                                                                                     >  1:2151846/1‑150 (MQ=255)
                                                tGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggt                                                                                                  <  2:323000/150‑1 (MQ=255)
                                                  cACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtgg                                                                                                <  2:1066450/150‑1 (MQ=255)
                                                  cACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtgg                                                                                                <  2:1648342/150‑1 (MQ=255)
                                                   aCGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGggtggt                                                                                               <  1:4706273/150‑1 (MQ=255)
                                                     ggTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGttt                                                                                             >  1:4485730/1‑150 (MQ=255)
                                                        aCGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTcc                                                                                          >  1:148749/1‑150 (MQ=255)
                                                           cTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCg                                                                                       >  2:3524494/1‑150 (MQ=255)
                                                           cTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCg                                                                                       <  2:3423617/150‑1 (MQ=255)
                                                             tCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACACATGGCGTCCAGCAGGGTGGTTTTCCCCGCa                                                                                     >  2:1040436/1‑150 (MQ=255)
                                                               aCCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCAcc                                                                                   <  1:2887526/150‑1 (MQ=255)
                                                                ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                  <  1:429140/150‑1 (MQ=255)
                                                                ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                  <  1:2362195/150‑1 (MQ=255)
                                                                ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                  <  2:1879976/150‑1 (MQ=255)
                                                                ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                  <  1:2802540/150‑1 (MQ=255)
                                                                ccTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCt                                                                                  <  2:2146709/150‑1 (MQ=255)
                                                                 cTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTg                                                                                 <  2:3607677/150‑1 (MQ=255)
                                                                       cTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGc                                                                           >  2:1250188/1‑150 (MQ=255)
                                                                          cAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCgg                                                                        >  2:2102108/1‑150 (MQ=255)
                                                                           aaaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGt                                                                       >  1:2541962/1‑150 (MQ=255)
                                                                           aaaCTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGt                                                                       >  1:1563579/1‑150 (MQ=255)
                                                                               tCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATa                                                                   <  1:1019128/150‑1 (MQ=255)
                                                                               tCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATa                                                                   <  1:1114179/150‑1 (MQ=255)
                                                                                  aCCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCa                                                                >  1:1856238/1‑150 (MQ=255)
                                                                                   ccTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCaa                                                               <  1:3810589/150‑1 (MQ=255)
                                                                                    cTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCaaa                                                              <  1:546999/150‑1 (MQ=255)
                                                                                     tCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAAc                                                             >  2:349979/1‑150 (MQ=255)
                                                                                      ccGACAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGAGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACa                                                            <  2:2843994/150‑1 (MQ=255)
                                                                                         ccAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGcc                                                         >  1:737446/1‑150 (MQ=255)
                                                                                          cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                        <  1:2073850/150‑1 (MQ=255)
                                                                                          cAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGccc                                                        <  1:2750957/150‑1 (MQ=255)
                                                                                               aTTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGc                                                   >  2:3794614/1‑150 (MQ=255)
                                                                                                ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCt                                                  <  1:1360888/150‑1 (MQ=255)
                                                                                                ttCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCt                                                  <  1:3678920/150‑1 (MQ=255)
                                                                                                 tCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTg                                                 <  1:1027063/150‑1 (MQ=255)
                                                                                                 tCGGCGGTATCGCGGGTCAAGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTg                                                 <  2:1451793/150‑1 (MQ=255)
                                                                                                   ggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                               <  2:4671098/150‑1 (MQ=255)
                                                                                                   ggcggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGc                                               <  1:4540233/150‑1 (MQ=255)
                                                                                                     cggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGa                                             <  2:2954160/150‑1 (MQ=255)
                                                                                                        tATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACg                                          <  1:3233379/150‑1 (MQ=255)
                                                                                                           cgcgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCt                                       <  2:3915310/150‑1 (MQ=255)
                                                                                                            gcgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTc                                      <  2:1127749/150‑1 (MQ=255)
                                                                                                             cgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcg                                     >  1:4025881/1‑150 (MQ=255)
                                                                                                             cgGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcg                                     >  2:601929/1‑150 (MQ=255)
                                                                                                              gggTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcgc                                    <  1:4490659/150‑1 (MQ=255)
                                                                                                               ggTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTcgcg                                   <  1:1289006/150‑1 (MQ=255)
                                                                                                                gTCATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCgg                                  >  2:822681/1‑150 (MQ=255)
                                                                                                                  cATGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGt                                >  2:1876664/1‑150 (MQ=255)
                                                                                                                    tGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                              <  2:2502831/150‑1 (MQ=255)
                                                                                                                    tGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                              <  2:128582/150‑1 (MQ=255)
                                                                                                                    tGAGATCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGa                              <  2:4363515/150‑1 (MQ=255)
                                                                                                                      agaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGaaa                            >  1:1714149/1‑150 (MQ=255)
                                                                                                                       gaTCATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaat                           <  1:3632949/150‑1 (MQ=255)
                                                                                                                          cATTTTGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaa                        <  2:2655672/150‑1 (MQ=255)
                                                                                                                            ttttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                      >  1:1177202/1‑150 (MQ=255)
                                                                                                                             tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAACTGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCt                     <  2:903232/150‑1 (MQ=255)
                                                                                                                             tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCt                     >  1:1197430/1‑150 (MQ=255)
                                                                                                                             tttGCGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCt                     >  1:4371013/1‑150 (MQ=255)
                                                                                                                                 cGATTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCa                 <  1:2158906/150‑1 (MQ=255)
                                                                                                                                   aTTGTGAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt               >  2:2790424/1‑150 (MQ=255)
                                                                                                                                     tgtgAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCg             >  1:660831/1‑150 (MQ=255)
                                                                                                                                        gAAACGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCt          >  1:1647927/1‑150 (MQ=255)
                                                                                                                                         aaaCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtt         <  2:2438121/150‑1 (MQ=255)
                                                                                                                                           aCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt       <  2:277198/150‑1 (MQ=255)
                                                                                                                                           aCGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCtttt       <  1:1233052/150‑1 (MQ=255)
                                                                                                                                            cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa      <  2:4429473/150‑1 (MQ=255)
                                                                                                                                            cGTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa      <  2:4485681/150‑1 (MQ=255)
                                                                                                                                             gTTAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTaa     <  1:4215885/150‑1 (MQ=255)
                                                                                                                                              ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt    >  2:230009/1‑150 (MQ=255)
                                                                                                                                              ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt    >  1:2694339/1‑150 (MQ=255)
                                                                                                                                              ttAGATAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt    >  1:319246/1‑150 (MQ=255)
                                                                                                                                               tAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATg   <  2:1504159/150‑1 (MQ=255)
                                                                                                                                               tAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAAGGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATg   <  2:2213101/150‑1 (MQ=255)
                                                                                                                                                agatagACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGa  <  1:794044/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCAAATTACCGTCGGGTTGGTTACGCGCCCGATTCTGGCTCCAGAATGCACGGTACGCTTCACCTTTCACTTCAAACTCCACCTCCGCCAGACATTCGGCGGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGA  >  CP009273/410880‑411173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑