Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 442,849 C→A 10.0% A105S (GCG→TCG)  cyoE ← protoheme IX farnesyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273442,8490CA10.0% 1402.7 / 93.0 393A105S (GCG→TCG) cyoEprotoheme IX farnesyltransferase
Reads supporting (aligned to +/- strand):  ref base C (152/201);  new base A (14/26);  total (166/227)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 410 total aligned reads displayed.

TACCGCACAGTAGCCGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACA  >  CP009273/442700‑442998
                                                                                                                                                     |                                                                                                                                                     
tACCGCACAGTAGCCGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTcgc                                                                                                                                                       >  2:18181/1‑150 (MQ=255)
 aCCGCACAGTAGCCGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTcgcg                                                                                                                                                      <  2:317356/150‑1 (MQ=255)
   cGCACAGTAGCCGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGcc                                                                                                                                                    <  2:4227481/150‑1 (MQ=255)
      acaGTAGCCTATCACCGGCGGCGCAGCGCCGTAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGATATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCaaa                                                                                                                                                 <  1:3795903/150‑1 (MQ=255)
        aGTAGCCGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAAcc                                                                                                                                               <  2:1317727/150‑1 (MQ=255)
              ctaTCACCGGCGGCGCAGCGCGGGAGTGCGAACCAATCAACGTGCCGTAGACAGTGTGGCGTTTCATTTACTGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCAcagca                                                                                                                                         <  1:2950543/148‑1 (MQ=255)
              cGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCCGCAGGCCCGCGGAGTCGCGCCAAACCAcagca                                                                                                                                         >  2:19378/1‑150 (MQ=255)
               gATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCACCGTGCCGTAGACAGAGTGGCGTTTCTTGTACTGGCTTTAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCGAGCGGATTCGCGCCAAACCAcagcag                                                                                                                                        <  2:21753/150‑1 (MQ=255)
               gATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTcgcg                                                                                                                                                      <  1:4506374/136‑1 (MQ=255)
                   aCCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATa                                                                                                                                    <  2:4314937/150‑1 (MQ=255)
                    ccGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATaa                                                                                                                                   >  2:3960573/1‑150 (MQ=255)
                     cggcggCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGTGTGGCGTTTCTTGTACAGGATATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATaaa                                                                                                                                  <  1:2149633/150‑1 (MQ=255)
                          gcgcAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAg                                                                                                                             <  1:3544433/150‑1 (MQ=255)
                            gcAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCa                                                                                                                           >  2:677848/1‑150 (MQ=255)
                              aGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAAt                                                                                                                         >  1:2718487/1‑150 (MQ=255)
                               gcgcCGGAGAGCGAACAAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACTGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATa                                                                                                                        <  1:282340/150‑1 (MQ=255)
                                  ccGGAGAGCGAACCAATCAACGTGCCGTAGACAGTGTGGCGTTTCATGTACTGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATAccc                                                                                                                     <  1:706473/150‑1 (MQ=255)
                                   cGGAGAGCGAACCAATCAACGTGCCTTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCa                                                                                                                    <  2:2764872/150‑1 (MQ=255)
                                   cGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCCGCGGATTCGCGCCCAACCACAGCAGCATAAAGCCAGCCATAcccc                                                                                                                    >  1:3295387/1‑149 (MQ=255)
                                   cGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCa                                                                                                                    <  1:3101300/150‑1 (MQ=255)
                                    ggAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCCCAGCAGCATAAAGCCAGCAATACCCAg                                                                                                                   >  1:1385786/1‑150 (MQ=255)
                                     gagaGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGATATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGc                                                                                                                  <  1:1931096/150‑1 (MQ=255)
                                      agagCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTAATGTAAAGGCTATAAACGCCGCCAAAAAACACAAAGCCCAACACCCCCACCCAGCACGCCCGCGGCATCGCGCCAAACCACAGCAGCAAAAAGCCAGCAAAACccacca                                                                                                                 >  1:3993709/1‑150 (MQ=255)
                                       gagCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCAACACCCCCAGCCACCAGGCCCGCGGATTCGCGCCCAACCACAGCAGCATAAAGCCAGCAATACCCAGcca                                                                                                                >  1:921640/1‑148 (MQ=255)
                                        agCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAAc                                                                                                               >  1:4289129/1‑150 (MQ=255)
                                        agCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAAc                                                                                                               <  2:3904869/150‑1 (MQ=255)
                                         gCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGAGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACg                                                                                                              <  2:634637/150‑1 (MQ=255)
                                           gAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATTTACAGGCTATAAAAGCCGACATAAACCACAAATCCCATCACCCCCACCCAGCAGGCCAGCGGATTCGCGCCAAACCACACCAGCATAAAGCCAGCAAAACCCAGCAACggg                                                                                                            >  1:2421908/1‑148 (MQ=255)
                                           gAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGGTTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTg                                                                                                            <  2:1025099/150‑1 (MQ=255)
                                             aCCAATCAACGTGCCGTAGACAGAGTGGCTTTTCATTTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGc                                                                                                          <  2:3624609/150‑1 (MQ=255)
                                               cAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGAGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGt                                                                                                        >  2:775142/1‑150 (MQ=255)
                                                   cAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTacac                                                                                                    <  2:4748354/150‑1 (MQ=255)
                                                    aaCGTGCCGTAGACAGAGTGGCGTTTCATTTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACAcc                                                                                                   <  1:903359/150‑1 (MQ=255)
                                                           cGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGCTTCGCGCCCAACCACAGCAGCATAAAGCCAGCACTACCCAGCAACGTGGCGTACACCAGCgaga                                                                                            >  1:1698650/1‑150 (MQ=255)
                                                              aGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCCGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGAcag                                                                                         >  2:506129/1‑150 (MQ=255)
                                                               gagtgagTGGCGTTTCTTGTACAGGCTTTCAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGAcagg                                                                                        <  2:3620233/146‑2 (MQ=255)
                                                               gACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGAcagc                                                                                        <  2:25594/150‑1 (MQ=255)
                                                                       ggCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGgagaga                                                                                <  1:3902780/150‑1 (MQ=255)
                                                                       ggCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGgagaga                                                                                >  2:4532332/1‑150 (MQ=255)
                                                                           tttCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAg                                                                            <  2:3121857/150‑1 (MQ=255)
                                                                                tGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGAGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCtt                                                                       <  1:4524704/150‑1 (MQ=255)
                                                                                  tACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTc                                                                     <  2:1900514/150‑1 (MQ=255)
                                                                                  tACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTc                                                                     <  2:2684363/150‑1 (MQ=255)
                                                                                      ggCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCa                                                                 >  2:4139399/1‑150 (MQ=255)
                                                                                         tataAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCa                                                              <  1:3960573/150‑1 (MQ=255)
                                                                                          ataAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCAc                                                             >  1:1155095/1‑150 (MQ=255)
                                                                                          ataAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCAc                                                             <  1:490761/150‑1 (MQ=255)
                                                                                          ataAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCAc                                                             >  2:4688208/1‑150 (MQ=255)
                                                                                            aaaCGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCAccc                                                           >  1:3854136/1‑150 (MQ=255)
                                                                                            aaaCGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCAccc                                                           <  1:2897903/150‑1 (MQ=255)
                                                                                             aaCGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCg                                                          >  2:2938240/1‑150 (MQ=255)
                                                                                             aaCGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCg                                                          <  1:443966/150‑1 (MQ=255)
                                                                                             aaCGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCg                                                          <  1:769764/150‑1 (MQ=255)
                                                                                               cGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGAGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAt                                                        <  2:4395018/150‑1 (MQ=255)
                                                                                                 ccGACATAAACCACAAAGACCATCACCCCCAGCCAGCAGGCCAGCTGATTCGCTCAAAACCACATCCGCATAAAGACAGCAATACCCAGCAACGTGGCGTACAACAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttc                                                      <  2:3829455/150‑1 (MQ=255)
                                                                                                   gACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt                                                    >  1:259550/1‑150 (MQ=255)
                                                                                                   gACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt                                                    <  1:1536406/150‑1 (MQ=255)
                                                                                                   gACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt                                                    >  2:3206845/1‑150 (MQ=255)
                                                                                                   gACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt                                                    <  2:1395304/150‑1 (MQ=255)
                                                                                                   gACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt                                                    <  2:111914/150‑1 (MQ=255)
                                                                                                    aCATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttcttc                                                   >  1:1002975/1‑150 (MQ=255)
                                                                                                     cATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCg                                                  >  2:2649736/1‑150 (MQ=255)
                                                                                                     cATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGAGTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAGTCTTCg                                                  >  2:197694/1‑150 (MQ=255)
                                                                                                      aTAAACCACAAAGCCCATCACCCCCCGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGt                                                 <  2:2611538/150‑1 (MQ=255)
                                                                                                      aTAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGt                                                 <  2:815233/150‑1 (MQ=255)
                                                                                                      aTAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGt                                                 >  2:2994182/1‑150 (MQ=255)
                                                                                                       tAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTc                                                >  2:3010952/1‑150 (MQ=255)
                                                                                                           ccACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGTAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCttt                                            <  1:2625419/150‑1 (MQ=255)
                                                                                                             acaAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTcc                                          >  1:1957494/1‑150 (MQ=255)
                                                                                                             acaAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTcc                                          >  2:285017/1‑150 (MQ=255)
                                                                                                               aaaGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCAt                                        >  1:3195479/1‑150 (MQ=255)
                                                                                                                  gCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCtt                                     <  2:1207218/150‑1 (MQ=255)
                                                                                                                     cATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCt                                  >  2:4574038/1‑150 (MQ=255)
                                                                                                                     cATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCt                                  <  2:152737/150‑1 (MQ=255)
                                                                                                                      aTCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTg                                 >  1:3768259/1‑150 (MQ=255)
                                                                                                                        cACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc                               <  1:1792679/150‑1 (MQ=255)
                                                                                                                          cccccAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGa                             <  1:4198234/150‑1 (MQ=255)
                                                                                                                           ccccCGCCAGCAGGCCAGCGGATTCGAGCCACACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGat                            <  2:2052035/150‑1 (MQ=255)
                                                                                                                           ccccAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGat                            <  2:4243316/150‑1 (MQ=255)
                                                                                                                            cccAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGata                           >  1:4069610/1‑150 (MQ=255)
                                                                                                                             ccagccagCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGatat                          <  1:4590306/150‑1 (MQ=255)
                                                                                                                                gccagcAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATccc                       >  1:2410283/1‑150 (MQ=255)
                                                                                                                                gccagcAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATccc                       <  1:2798342/150‑1 (MQ=255)
                                                                                                                                 ccagcAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCt                      <  2:426865/150‑1 (MQ=255)
                                                                                                                                  cagcagGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTg                     >  1:1855268/1‑150 (MQ=255)
                                                                                                                                   agcagGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGt                    >  2:3333534/1‑150 (MQ=255)
                                                                                                                                   agcagGCCAGCGGATTCGAGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGt                    >  1:1955566/1‑150 (MQ=255)
                                                                                                                                    gcagGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTc                   <  1:3395483/150‑1 (MQ=255)
                                                                                                                                     cagGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCg                  <  1:3130870/150‑1 (MQ=255)
                                                                                                                                      agGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGa                 >  2:868766/1‑150 (MQ=255)
                                                                                                                                       ggCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGAt                <  2:2464176/150‑1 (MQ=255)
                                                                                                                                        gCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATg               >  2:4016263/1‑150 (MQ=255)
                                                                                                                                           aGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg            >  2:4458830/1‑150 (MQ=255)
                                                                                                                                           aGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg            <  1:2108618/150‑1 (MQ=255)
                                                                                                                                           aGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg            <  2:2790800/150‑1 (MQ=255)
                                                                                                                                             cGGATTCGCGCCAAACCACCGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAgtt          <  1:1284878/150‑1 (MQ=255)
                                                                                                                                                 ttCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTa      <  2:2334855/150‑1 (MQ=255)
                                                                                                                                                   cgcgCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATCTCCCTGTCGATGTAGTTGTTaaa    >  2:4433090/1‑150 (MQ=255)
                                                                                                                                                     cgcCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAaca  >  1:235570/1‑150 (MQ=255)
                                                                                                                                                     cgcCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAaca  >  1:1430980/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACCGCACAGTAGCCGATCACCGGCGGCGCAGCGCCGGAGAGCGAACCAATCAACGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACA  >  CP009273/442700‑442998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑