Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 534,666 G→A 12.2% D22N (GAT→AAT)  allB → allantoinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273534,6660GA12.2% 644.7 / 61.1 213D22N (GAT→AAT) allBallantoinase
Reads supporting (aligned to +/- strand):  ref base G (125/62);  new base A (15/11);  total (140/73)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

AGGAGAGCAAAAAACCATAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACTGGGAA  >  CP009273/534519‑534815
                                                                                                                                                   |                                                                                                                                                     
agcagagCAAAAAACCATAGTTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGat                                                                                                                                                     <  1:272516/147‑1 (MQ=255)
aGGAGAGCAAAAAACCATAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTATAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGat                                                                                                                                                     >  2:4048280/1‑150 (MQ=255)
aGGAGAGCAAAAAACCATAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGat                                                                                                                                                     >  1:1445635/1‑150 (MQ=255)
        aaaaaaCCATAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGt                                                                                                                                             >  1:2388434/1‑150 (MQ=255)
          aaaaCCATAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCACGCGTTGTAGATATCGCCGTTa                                                                                                                                           >  2:2895386/1‑150 (MQ=255)
              ccATAGGTTAATTACTCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAgg                                                                                                                                       <  2:516283/150‑1 (MQ=255)
                aTAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCg                                                                                                                                     <  2:1326664/150‑1 (MQ=255)
                aTAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGGTAAAGGCg                                                                                                                                     >  2:2796235/1‑150 (MQ=255)
                  aGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGa                                                                                                                                   >  1:3191950/1‑150 (MQ=255)
                   ggTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGaa                                                                                                                                  >  1:1302037/1‑150 (MQ=255)
                     ttaattaaTCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGaaaa                                                                                                                                <  1:3350386/150‑1 (MQ=255)
                           aatCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATtgct                                                                                                                          >  1:999567/1‑150 (MQ=255)
                            atCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATtgctg                                                                                                                         >  2:1907287/1‑150 (MQ=255)
                            atCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATtgctg                                                                                                                         >  2:3372999/1‑150 (MQ=255)
                             tCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATtgctgc                                                                                                                        <  2:3092154/150‑1 (MQ=255)
                             tCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATtgctgc                                                                                                                        <  2:4662673/150‑1 (MQ=255)
                                   tatTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCgg                                                                                                                  >  2:149921/1‑150 (MQ=255)
                                   tatTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCgg                                                                                                                  >  1:887455/1‑150 (MQ=255)
                                   tatTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCgg                                                                                                                  >  2:4396927/1‑150 (MQ=255)
                                    atTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGt                                                                                                                 >  1:1727058/1‑150 (MQ=255)
                                     ttGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTc                                                                                                                >  2:2054963/1‑150 (MQ=255)
                                     ttGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTc                                                                                                                >  2:1739552/1‑150 (MQ=255)
                                      ttaaCATTGAGTTAAAAACCAATCTTTATTTTACATGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCa                                                                                                               <  1:3620297/148‑1 (MQ=255)
                                         aCATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGa                                                                                                            >  2:2008600/1‑150 (MQ=255)
                                            ttGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCt                                                                                                         >  2:549685/1‑150 (MQ=255)
                                            ttGAGTAAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCt                                                                                                         <  2:2615760/150‑1 (MQ=255)
                                             tGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTg                                                                                                        <  2:3804835/150‑1 (MQ=255)
                                                 ttAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCg                                                                                                    >  2:2947955/1‑150 (MQ=255)
                                                          aaTCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAaag                                                                                           <  1:2755601/150‑1 (MQ=255)
                                                          aaTCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAaag                                                                                           >  1:1316190/1‑150 (MQ=255)
                                                              tGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCCCCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGt                                                                                       >  2:2670506/1‑150 (MQ=255)
                                                               gTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGtt                                                                                      >  2:2971755/1‑150 (MQ=255)
                                                                 aTTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTAt                                                                                    >  2:259615/1‑150 (MQ=255)
                                                                      aCAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATg                                                                               >  1:2656165/1‑150 (MQ=255)
                                                                       cAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGc                                                                              >  1:4236085/1‑150 (MQ=255)
                                                                        aaGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATTGATGCg                                                                             >  1:2612816/1‑150 (MQ=255)
                                                                        aaGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCg                                                                             >  1:725845/1‑150 (MQ=255)
                                                                         agtaGTTTGTTATGTATTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCgt                                                                            <  2:3966007/147‑1 (MQ=255)
                                                                         aGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGATAAAGGCGGAAAAATTCCTGCTATCGGTCACGAACTGGGCGAAGCAAAACAAGTTATGGATGCgt                                                                            >  2:6893/1‑150 (MQ=255)
                                                                           gAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCgtct                                                                          <  2:2145672/150‑1 (MQ=255)
                                                                            aGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCgtctg                                                                         >  2:2422017/1‑150 (MQ=255)
                                                                              tttGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCgtctggt                                                                       <  1:1131501/150‑1 (MQ=255)
                                                                                tGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCgtctggtct                                                                     >  1:1822265/1‑150 (MQ=255)
                                                                                tGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCgtctggtct                                                                     >  1:1863666/1‑150 (MQ=255)
                                                                                   tATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTctggtctggt                                                                  >  1:1964268/1‑150 (MQ=255)
                                                                                    aTGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCtggtg                                                                 >  1:3219411/1‑150 (MQ=255)
                                                                                    aTGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCtggtg                                                                 <  2:557618/150‑1 (MQ=255)
                                                                                    aTGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCtggtg                                                                 >  1:1080500/1‑150 (MQ=255)
                                                                                     tGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCtggtgg                                                                >  1:2490483/1‑150 (MQ=255)
                                                                                       tCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGtt                                                              <  1:4516465/150‑1 (MQ=255)
                                                                                       tCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGtt                                                              >  2:1817489/1‑150 (MQ=255)
                                                                                        cTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGttt                                                             <  2:4116297/150‑1 (MQ=255)
                                                                                         ttttGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTc                                                            >  1:1345228/1‑150 (MQ=255)
                                                                                         ttttGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTc                                                            >  2:3527865/1‑150 (MQ=255)
                                                                                             gATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCg                                                        >  1:3954738/1‑150 (MQ=255)
                                                                                             gATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCg                                                        >  2:544587/1‑150 (MQ=255)
                                                                                               tttAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCggg                                                      >  2:497946/1‑150 (MQ=255)
                                                                                                ttAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGc                                                     >  1:149442/1‑150 (MQ=255)
                                                                                                ttAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGc                                                     >  1:3309113/1‑150 (MQ=255)
                                                                                                  aaTCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCAt                                                   >  2:2494097/1‑150 (MQ=255)
                                                                                                   aTCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCCTg                                                  >  2:4732407/1‑150 (MQ=255)
                                                                                                   aTCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATg                                                  <  1:281850/150‑1 (MQ=255)
                                                                                                   aTCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATg                                                  <  1:282554/150‑1 (MQ=255)
                                                                                                   aTCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATg                                                  <  2:235590/150‑1 (MQ=255)
                                                                                                     cATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGt                                                <  1:4209527/150‑1 (MQ=255)
                                                                                                     cATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGt                                                >  1:2268001/1‑150 (MQ=255)
                                                                                                       ttAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGTGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTg                                              >  2:1449643/1‑150 (MQ=255)
                                                                                                       ttAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTg                                              >  2:3842613/1‑150 (MQ=255)
                                                                                                         aaaaaCGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGAt                                            >  2:4286132/1‑150 (MQ=255)
                                                                                                         aaaaaCGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGAt                                            <  1:4429002/150‑1 (MQ=255)
                                                                                                           aaaCGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATgc                                          <  1:3642686/150‑1 (MQ=255)
                                                                                                            aaCGGCACCGTTATTTTAGAACACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATgcg                                         <  2:1281355/150‑1 (MQ=255)
                                                                                                               gtcACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCATTTAAAGGCGGAAAAAATGCTGCTATCGGTCAGGATCTGGGCGATTCAAAAGAAGTTATGGATGCGTCAGGTAGGGTGGTTTCGCCGGGCATGGATGATGCGcac                                      >  2:3280623/3‑150 (MQ=255)
                                                                                                               ggCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGcac                                      <  2:816853/150‑1 (MQ=255)
                                                                                                                 cACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCacac                                    >  2:3148933/1‑150 (MQ=255)
                                                                                                                 cACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCacac                                    >  2:3148858/1‑150 (MQ=255)
                                                                                                                    cGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCa                                 >  1:3684539/1‑150 (MQ=255)
                                                                                                                      ttATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCata                               >  1:1046106/1‑150 (MQ=255)
                                                                                                                      ttATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCata                               <  2:1181043/150‑1 (MQ=255)
                                                                                                                      ttATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCata                               >  1:2118869/1‑150 (MQ=255)
                                                                                                                         ttttAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATAttt                            <  2:3191950/150‑1 (MQ=255)
                                                                                                                         ttttAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATAttt                            >  1:1392495/1‑150 (MQ=255)
                                                                                                                             aGAAAACGAAGCTCGCGTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGa                        >  1:2373524/1‑150 (MQ=255)
                                                                                                                                aaaCGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAAcc                     <  1:4145034/150‑1 (MQ=255)
                                                                                                                                 aaCGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCg                    >  1:3453984/1‑150 (MQ=255)
                                                                                                                                  aCGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTCTCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGAGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCgg                   >  2:1810404/1‑150 (MQ=255)
                                                                                                                                        cTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTa             <  2:2936384/150‑1 (MQ=255)
                                                                                                                                        cTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTa             <  2:1542439/150‑1 (MQ=255)
                                                                                                                                        cTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTa             >  2:4681255/1‑150 (MQ=255)
                                                                                                                                         tCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAg            >  1:1039806/1‑150 (MQ=255)
                                                                                                                                          cgcgTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGc           <  1:3372999/150‑1 (MQ=255)
                                                                                                                                          cgcgTTGTAAATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGc           <  1:1907287/150‑1 (MQ=255)
                                                                                                                                           gcgTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGcc          <  2:1036000/150‑1 (MQ=255)
                                                                                                                                            cgTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCa         >  1:1809397/1‑150 (MQ=255)
                                                                                                                                             gTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTAGTAGCCAc        >  2:4619630/1‑150 (MQ=255)
                                                                                                                                              ttGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACt       <  1:2971755/150‑1 (MQ=255)
                                                                                                                                               tGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACTg      >  2:1855388/1‑150 (MQ=255)
                                                                                                                                                  aGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACTGGGa   >  1:3491445/1‑150 (MQ=255)
                                                                                                                                                   gATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACTGGGaa  >  2:4130846/1‑150 (MQ=255)
                                                                                                                                                   gATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACTGGGaa  >  2:3361210/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGGAGAGCAAAAAACCATAGGTTAATTAATCCCGATATTGAACATTGAGTTAAAAACCAATCTGTATTTTACAAGGAGTTTGTTATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTTGTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCAAAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACCCATATTTCTGAACCGGGTCGTAGCCACTGGGAA  >  CP009273/534519‑534815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑