Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 550,786 G→T 14.2% A240A (GCG→GCT)  cysS → cysteinyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273550,7860GT14.2% 846.3 / 94.3 261A240A (GCG→GCT) cysScysteinyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (102/122);  new base T (11/26);  total (113/148)
Fisher's exact test for biased strand distribution p-value = 7.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 271 total aligned reads displayed.

GAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCG  >  CP009273/550637‑550935
                                                                                                                                                     |                                                                                                                                                     
gAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATcgcg                                                                                                                                                       >  1:4112524/1‑150 (MQ=255)
gAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATcgcg                                                                                                                                                       >  1:498603/1‑150 (MQ=255)
gAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATcgcg                                                                                                                                                       >  2:1898849/1‑150 (MQ=255)
    cGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGt                                                                                                                                                   >  2:1617013/1‑150 (MQ=255)
     gAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACATTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTc                                                                                                                                                  >  2:3880258/1‑150 (MQ=255)
      aGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTcc                                                                                                                                                 <  1:1032257/150‑1 (MQ=255)
       gCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCa                                                                                                                                                <  1:2829111/150‑1 (MQ=255)
       gCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCa                                                                                                                                                <  1:2199369/150‑1 (MQ=255)
         tGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc                                                                                                                                              <  2:1772643/150‑1 (MQ=255)
           gCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCtg                                                                                                                                            <  1:2132934/150‑1 (MQ=255)
           gCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCtg                                                                                                                                            <  2:2228962/150‑1 (MQ=255)
            ccGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGTGTAACCACTTTTATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCTACACTATAACGAAATCGCTCAGTCCACCtgt                                                                                                                                           <  1:3888749/150‑1 (MQ=255)
               tctcCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGcc                                                                                                                                        <  2:3528156/150‑1 (MQ=255)
               tctcCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGcc                                                                                                                                        <  2:2271543/150‑1 (MQ=255)
                  ccGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCat                                                                                                                                     <  2:4290924/150‑1 (MQ=255)
                   cGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCatg                                                                                                                                    >  2:2598000/1‑150 (MQ=255)
                   cGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatg                                                                                                                                    <  1:392949/150‑1 (MQ=255)
                   cGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGGTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatg                                                                                                                                    <  2:2309065/150‑1 (MQ=255)
                    gTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatga                                                                                                                                   >  2:4427272/1‑150 (MQ=255)
                     tGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCatgat                                                                                                                                  <  2:4544939/150‑1 (MQ=255)
                     tGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatgat                                                                                                                                  >  1:3775114/1‑150 (MQ=255)
                      ggggCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatgatg                                                                                                                                 <  1:3820095/150‑1 (MQ=255)
                         gcgcGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc                                                                                                                              <  1:985979/150‑1 (MQ=255)
                          cgcgGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCa                                                                                                                             >  1:4637595/1‑150 (MQ=255)
                          cgcgGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCa                                                                                                                             <  1:4668413/150‑1 (MQ=255)
                              ggTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTAt                                                                                                                         <  1:1576994/150‑1 (MQ=255)
                              ggTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTAt                                                                                                                         <  1:507102/150‑1 (MQ=255)
                               gtcgtcCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTAtg                                                                                                                        >  2:3003404/1‑150 (MQ=255)
                                   tcCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGaa                                                                                                                    <  2:1984815/150‑1 (MQ=255)
                                     ctggctggCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAact                                                                                                                  <  2:2030358/150‑1 (MQ=255)
                                     ctggctggCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAact                                                                                                                  >  1:1363885/1‑150 (MQ=255)
                                          tggcACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTgg                                                                                                             >  1:3754542/1‑150 (MQ=255)
                                            gcACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGAt                                                                                                           >  1:1221098/1‑150 (MQ=255)
                                            gcACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGGTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGAt                                                                                                           <  2:2865079/150‑1 (MQ=255)
                                             cacaTTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATg                                                                                                          >  2:4700686/1‑150 (MQ=255)
                                                 ttGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACt                                                                                                      >  2:2881864/1‑150 (MQ=255)
                                                  tGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTc                                                                                                     <  1:871892/150‑1 (MQ=255)
                                                    aaTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCgg                                                                                                   >  1:4269606/1‑150 (MQ=255)
                                                    aaTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCgg                                                                                                   <  2:4147187/150‑1 (MQ=255)
                                                     aTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCggg                                                                                                  >  1:2372899/1‑150 (MQ=255)
                                                       gTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGga                                                                                                <  1:28831/150‑1 (MQ=255)
                                                       gTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGga                                                                                                >  1:2268934/1‑150 (MQ=255)
                                                       gTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGga                                                                                                <  1:2279867/150‑1 (MQ=255)
                                                       gTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGga                                                                                                <  1:781848/150‑1 (MQ=255)
                                                         tCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGgatg                                                                                              >  2:4052256/1‑150 (MQ=255)
                                                           ggCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGgatggt                                                                                            >  2:3653842/1‑150 (MQ=255)
                                                              aaTGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGgatggtgat                                                                                         <  1:4036044/150‑1 (MQ=255)
                                                              aaTGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGgatggtgat                                                                                         >  2:3676642/1‑150 (MQ=255)
                                                               aTGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGgatggtgatg                                                                                        <  1:3019920/150‑1 (MQ=255)
                                                                tGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGgatggtgatgg                                                                                       <  2:1798074/150‑1 (MQ=255)
                                                                   aCTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTg                                                                                    <  1:4013737/150‑1 (MQ=255)
                                                                   aCTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTg                                                                                    >  2:2125211/1‑150 (MQ=255)
                                                                     tGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGAc                                                                                  <  1:4212658/150‑1 (MQ=255)
                                                                      gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGAcc                                                                                 <  2:65123/150‑1 (MQ=255)
                                                                           cagcTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCgag                                                                            >  1:2753395/1‑150 (MQ=255)
                                                                           cagcTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCgag                                                                            >  1:2753482/1‑150 (MQ=255)
                                                                             gcTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGagaa                                                                          <  2:1508387/150‑1 (MQ=255)
                                                                               tGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCAGGGATGGTGATGGTTGACCGCGagaaga                                                                        >  1:3226607/1‑150 (MQ=255)
                                                                                  gaaaCCACTTTGATATACACGGCGGCGGTTCATACCAGATGTTCACGCACCACGAAAAAGAAATCGCGCATTCCACCTGTCCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATCGTGATTGTTGACCGAGAGAAGATGt                                                                     >  2:3908399/3‑150 (MQ=255)
                                                                                    aaCCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTcc                                                                   >  1:3710735/1‑150 (MQ=255)
                                                                                      ccACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCaa                                                                 <  2:4719469/150‑1 (MQ=255)
                                                                                        aCTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAAt                                                               <  1:2598000/150‑1 (MQ=255)
                                                                                          tttGATATCCACGGCGGCGGTTCAGACCTGATGGTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCg                                                             <  1:146212/150‑1 (MQ=255)
                                                                                           ttGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGc                                                            >  2:619761/1‑150 (MQ=255)
                                                                                            tGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCt                                                           <  1:3103399/150‑1 (MQ=255)
                                                                                            tGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCt                                                           <  1:732536/150‑1 (MQ=255)
                                                                                             gATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTg                                                          <  2:85993/150‑1 (MQ=255)
                                                                                             gATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTg                                                          <  2:1542355/150‑1 (MQ=255)
                                                                                             gATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTg                                                          <  2:273657/150‑1 (MQ=255)
                                                                                             gATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTg                                                          <  2:4446739/150‑1 (MQ=255)
                                                                                             gATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTg                                                          <  1:602772/150‑1 (MQ=255)
                                                                                             gATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTg                                                          <  1:2974808/150‑1 (MQ=255)
                                                                                              atatCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTgg                                                         >  1:660974/1‑150 (MQ=255)
                                                                                              atatCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTgg                                                         >  2:4016139/1‑150 (MQ=255)
                                                                                                atCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGt                                                       <  1:3198543/150‑1 (MQ=255)
                                                                                                atCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGt                                                       <  1:1224973/150‑1 (MQ=255)
                                                                                                atCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGt                                                       <  2:4044386/150‑1 (MQ=255)
                                                                                                 tCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTa                                                      <  1:1028797/150‑1 (MQ=255)
                                                                                                     cggcggcggTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTCACCGCGAGAAGATGTCCAAATCGCTGGGTAActt                                                  <  1:4483245/150‑1 (MQ=255)
                                                                                                      ggcggcggTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAActtc                                                 >  2:2116781/1‑150 (MQ=255)
                                                                                                           cggTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTAc                                            >  2:1026611/1‑150 (MQ=255)
                                                                                                            ggTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTAcc                                           <  2:1157247/150‑1 (MQ=255)
                                                                                                                 aGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTgcg                                      <  2:309769/150‑1 (MQ=255)
                                                                                                                    ccTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGa                                   >  1:1843477/1‑150 (MQ=255)
                                                                                                                     cTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGAt                                  >  1:3441224/1‑150 (MQ=255)
                                                                                                                      tGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGAtg                                 >  2:2963913/1‑150 (MQ=255)
                                                                                                                        aTGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGAtgtg                               <  2:3754911/150‑1 (MQ=255)
                                                                                                                          gTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCt                             <  1:1646800/150‑1 (MQ=255)
                                                                                                                                gCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAta                       <  2:1714765/150‑1 (MQ=255)
                                                                                                                                 caccacGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtac                      <  1:2114945/150‑1 (MQ=255)
                                                                                                                                   ccacGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtacta                    >  1:3317621/1‑150 (MQ=255)
                                                                                                                                    cacGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAAtactac                   <  1:4405974/150‑1 (MQ=255)
                                                                                                                                       gaaaacgaaaTCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTacgac                <  2:2135476/150‑1 (MQ=255)
                                                                                                                                        aaaacgaaaTCGCTCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTacgacg               <  1:1946671/150‑1 (MQ=255)
                                                                                                                                          aacgaaaTCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGAcgcg             <  2:148897/150‑1 (MQ=255)
                                                                                                                                          aacgaaaTCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGAcgcg             <  2:84319/150‑1 (MQ=255)
                                                                                                                                            cgaaaTCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGa           >  2:3178770/1‑150 (MQ=255)
                                                                                                                                              aaaTCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGaaa         >  2:761217/1‑150 (MQ=255)
                                                                                                                                                   gcgcAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTg    >  1:3538810/1‑150 (MQ=255)
                                                                                                                                                   gcgcAGTCCACCGGTGCCCATGATGGTCAGTAGGTGAACTCCTGGAAGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTg    <  2:4656262/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCG  >  CP009273/550637‑550935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑