BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	583,341	T→G	73.7%	S33R (AGT→CGT)	ybcH ←	PRK09936 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	583,341	0	T	G	73.7%	625.5 / 208.1	279	S33R (AGT→CGT)	ybcH	PRK09936 family protein
Reads supporting (aligned to +/- strand): ref base T (35/39); new base G (92/113); total (127/152)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

GCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  >  CP009273/583193‑583490

gcAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTa                                                                                                                                                      <  1:4115687/150‑1 (MQ=255)

  aGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTAtc                                                                                                                                                    >  2:3642984/1‑150 (MQ=255)

  aGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAtc                                                                                                                                                    <  1:2974828/150‑1 (MQ=255)

    cTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACtctctc                                                                                                                                                  >  2:27362/1‑150 (MQ=255)

    cTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGGAACCTGACGAtctc                                                                                                                                                  <  2:1198085/150‑1 (MQ=255)

     tGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCg                                                                                                                                                 >  2:3464847/1‑150 (MQ=255)

        ggCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtt                                                                                                                                              >  1:4047644/1‑150 (MQ=255)

          cccGCTTAAACAAGAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtttt                                                                                                                                            <  2:2157911/150‑1 (MQ=255)

           ccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtg                                                                                                                                           >  2:563343/1‑150 (MQ=255)

           ccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtg                                                                                                                                           >  2:2995237/1‑150 (MQ=255)

            cGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgt                                                                                                                                          <  2:39082/150‑1 (MQ=255)

            cGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgt                                                                                                                                          <  2:1688040/150‑1 (MQ=255)

                 aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                     <  1:2345110/150‑1 (MQ=255)

                 aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                     <  1:3935279/150‑1 (MQ=255)

                  aaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGc                                                                                                                                    >  2:4538464/1‑150 (MQ=255)

                   aCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGcc                                                                                                                                   <  1:3438205/150‑1 (MQ=255)

                     aaTAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAg                                                                                                                                 >  1:3030539/1‑150 (MQ=255)

                       tAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGat                                                                                                                               >  2:1661031/1‑150 (MQ=255)

                       tAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGat                                                                                                                               <  1:3756672/150‑1 (MQ=255)

                        aaCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGata                                                                                                                              <  2:3604500/150‑1 (MQ=255)

                        aaCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGata                                                                                                                              >  1:4184594/1‑150 (MQ=255)

                        aaCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGata                                                                                                                              <  2:2638029/150‑1 (MQ=255)

                           gTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGataata                                                                                                                           <  1:972671/150‑1 (MQ=255)

                           gTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGataata                                                                                                                           >  1:4754674/1‑150 (MQ=255)

                            tGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAc                                                                                                                          <  2:2388122/150‑1 (MQ=255)

                              cgcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCt                                                                                                                        >  1:2694900/1‑150 (MQ=255)

                              cgcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCt                                                                                                                        >  1:674579/1‑150 (MQ=255)

                                 tGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTc                                                                                                                     >  1:1745892/1‑150 (MQ=255)

                                  gTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCa                                                                                                                    >  2:1468318/1‑150 (MQ=255)

                                  gTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCa                                                                                                                    >  2:1348327/1‑150 (MQ=255)

                                    tCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATc                                                                                                                  <  1:3151680/150‑1 (MQ=255)

                                       ggctggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATc                                                                                                                  <  2:2784283/147‑1 (MQ=255)

                                       ggctggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCa                                                                                                               <  2:4071990/150‑1 (MQ=255)

                                         ctggGTAAATGCATCGCCGTACCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTACCCTGACGAGCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaaa                                                                                                             >  2:3136195/1‑150 (MQ=255)

                                         ctggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaaa                                                                                                             >  2:211002/1‑150 (MQ=255)

                                         ctggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaaa                                                                                                             <  2:451397/150‑1 (MQ=255)

                                             gTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaa                                                                                                         <  1:2512599/150‑1 (MQ=255)

                                              tAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaa                                                                                                        <  2:801555/150‑1 (MQ=255)

                                               aaaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaa                                                                                                       >  2:1426427/1‑150 (MQ=255)

                                               aaaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaa                                                                                                       >  1:4688118/1‑150 (MQ=255)

                                                aaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaaa                                                                                                      <  2:4577254/150‑1 (MQ=255)

                                                  tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                                    <  1:1024780/150‑1 (MQ=255)

                                                  tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                                    <  1:1022497/150‑1 (MQ=255)

                                                   gCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAggg                                                                                                   <  1:1503990/150‑1 (MQ=255)

                                                   gCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAggg                                                                                                   >  1:1529042/1‑150 (MQ=255)

                                                    cATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGc                                                                                                  <  1:3634515/150‑1 (MQ=255)

                                                       cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                               <  1:3143921/150‑1 (MQ=255)

                                                       cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                               >  1:2882204/1‑150 (MQ=255)

                                                       cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                               <  1:3492786/150‑1 (MQ=255)

                                                        gCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGAc                                                                                              >  1:4663687/1‑150 (MQ=255)

                                                          cGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                            <  1:3198395/150‑1 (MQ=255)

                                                          cGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                            <  2:3883691/150‑1 (MQ=255)

                                                            tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaa                                                                                          >  1:4536606/1‑150 (MQ=255)

                                                             aaCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaaa                                                                                         >  1:2786990/1‑150 (MQ=255)

                                                               cGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGc                                                                                       >  2:1841884/1‑150 (MQ=255)

                                                                gggTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCa                                                                                      >  2:4476457/1‑150 (MQ=255)

                                                                gggTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCa                                                                                      <  2:2849781/150‑1 (MQ=255)

                                                                   tCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgtg                                                                                   <  2:197707/150‑1 (MQ=255)

                                                                     cATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTc                                                                                 >  1:387950/1‑150 (MQ=255)

                                                                     cATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTc                                                                                 >  1:3799317/1‑150 (MQ=255)

                                                                     cATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTc                                                                                 <  2:2280119/150‑1 (MQ=255)

                                                                      aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                                <  2:4839067/150‑1 (MQ=255)

                                                                        tGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGc                                                                              <  2:3211140/150‑1 (MQ=255)

                                                                           aaaaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAt                                                                           >  2:4261049/1‑150 (MQ=255)

                                                                              aCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACg                                                                        >  1:2379074/1‑150 (MQ=255)

                                                                                aaGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaa                                                                      >  1:4269391/1‑150 (MQ=255)

                                                                                 aGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                     >  1:518467/1‑150 (MQ=255)

                                                                                  gggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTACATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaaa                                                                    <  1:2673778/150‑1 (MQ=255)

                                                                                   ggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAAt                                                                   <  2:776281/150‑1 (MQ=255)

                                                                                      aTCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGaa                                                                >  1:3453099/1‑150 (MQ=255)

                                                                                         gAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACtt                                                             <  1:4295907/150‑1 (MQ=255)

                                                                                                  cAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTa                                                    <  1:3999755/150‑1 (MQ=255)

                                                                                                   aaaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAc                                                   <  1:1548540/150‑1 (MQ=255)

                                                                                                   aaaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAc                                                   >  2:2648075/1‑150 (MQ=255)

                                                                                                   aaaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAc                                                   >  2:1073275/1‑150 (MQ=255)

                                                                                                    aaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAcc                                                  >  2:2589752/1‑150 (MQ=255)

                                                                                                    aaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAcc                                                  >  1:927888/1‑150 (MQ=255)

                                                                                                    aaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAcc                                                  <  2:3501015/150‑1 (MQ=255)

                                                                                                       gTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGt                                               >  1:792946/1‑150 (MQ=255)

                                                                                                             gACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTc                                         <  1:1305162/150‑1 (MQ=255)

                                                                                                              aCTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTcc                                        >  2:3756704/1‑150 (MQ=255)

                                                                                                                  atcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATg                                    >  1:3554499/1‑150 (MQ=255)

                                                                                                                   tcaGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATgg                                   <  2:599323/150‑1 (MQ=255)

                                                                                                                     aGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTg                                 >  2:3301646/1‑150 (MQ=255)

                                                                                                                      gCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                             >  2:761115/1‑137 (MQ=255)

                                                                                                                        cccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTgaga                              <  1:563343/150‑1 (MQ=255)

                                                                                                                          cTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaaa                            >  1:3379202/1‑150 (MQ=255)

                                                                                                                                cTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgtt                      >  1:4501880/1‑150 (MQ=255)

                                                                                                                                     tATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgc                 <  1:4149900/150‑1 (MQ=255)

                                                                                                                                     tATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgc                 <  1:2651939/150‑1 (MQ=255)

                                                                                                                                     tATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAGGGTGAGAAACGCGTTGTTgc                 >  1:4724166/1‑150 (MQ=255)

                                                                                                                                        cGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGct              <  2:1936846/150‑1 (MQ=255)

                                                                                                                                         ggTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGctc             >  1:4422728/1‑150 (MQ=255)

                                                                                                                                           tAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCtctc           >  2:411538/1‑150 (MQ=255)

                                                                                                                                            aaCCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTcc          <  1:2207522/150‑1 (MQ=255)

                                                                                                                                            aaCCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTcc          <  1:1973787/150‑1 (MQ=255)

                                                                                                                                             aCCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCg         <  2:4436019/150‑1 (MQ=255)

                                                                                                                                               cTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGtt       >  2:2965393/1‑150 (MQ=255)

                                                                                                                                                tGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTa      >  1:1119804/1‑150 (MQ=255)

                                                                                                                                                  aCTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACg    >  2:2589172/1‑150 (MQ=255)

GCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  >  CP009273/583193‑583490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`