Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 754,572 G→T 69.5% E137D (GAG→GAT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273754,5720GT69.5% 341.7 / 289.6 269E137D (GAG→GAT) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (32/50);  new base T (98/89);  total (130/139)
Fisher's exact test for biased strand distribution p-value = 4.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 272 total aligned reads displayed.

AAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAA  >  CP009273/754423‑754716
                                                                                                                                                     |                                                                                                                                                
 aGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGgaga                                                                                                                                                 >  2:714338/1‑150 (MQ=255)
 aGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGgaga                                                                                                                                                 >  2:3144621/1‑150 (MQ=255)
    aGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCt                                                                                                                                              >  1:946815/1‑150 (MQ=255)
       ttAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCa                                                                                                                                           <  1:1323821/150‑1 (MQ=255)
       ttAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCa                                                                                                                                           <  2:1985678/150‑1 (MQ=255)
        tAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCaa                                                                                                                                          >  1:4751573/1‑150 (MQ=255)
        tAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCaa                                                                                                                                          >  1:4751717/1‑150 (MQ=255)
         aaaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAAc                                                                                                                                         <  1:1743819/150‑1 (MQ=255)
          aaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACg                                                                                                                                        >  1:2414430/1‑150 (MQ=255)
             tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCg                                                                                                                                     <  2:1701719/150‑1 (MQ=255)
                 gCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTc                                                                                                                                 <  2:2538548/150‑1 (MQ=255)
                     cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAttt                                                                                                                             <  2:2516616/150‑1 (MQ=255)
                            aCGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCg                                                                                                                      >  2:1493717/1‑150 (MQ=255)
                            aCGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCg                                                                                                                      >  2:3951514/1‑150 (MQ=255)
                             cGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCgg                                                                                                                     <  1:545076/150‑1 (MQ=255)
                             cGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCgg                                                                                                                     <  1:4208833/150‑1 (MQ=255)
                              gCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGc                                                                                                                    <  1:2504544/150‑1 (MQ=255)
                              gCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGc                                                                                                                    <  1:2506281/150‑1 (MQ=255)
                                aTACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCaa                                                                                                                  >  2:2669663/1‑150 (MQ=255)
                                aTACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCaa                                                                                                                  >  2:4608475/1‑150 (MQ=255)
                                 tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCaaa                                                                                                                 >  1:2265656/1‑150 (MQ=255)
                                     gCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGaaa                                                                                                             <  2:767322/150‑1 (MQ=255)
                                      cTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAc                                                                                                            >  1:329356/1‑150 (MQ=255)
                                         ccGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCAt                                                                                                         <  2:4302809/150‑1 (MQ=255)
                                         ccGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCAt                                                                                                         <  2:1476129/150‑1 (MQ=255)
                                             ggTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaaa                                                                                                     <  1:2915069/150‑1 (MQ=255)
                                                cACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTc                                                                                                  <  2:4424642/150‑1 (MQ=255)
                                                  ccAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAACGGGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCgg                                                                                                <  2:1357734/150‑1 (MQ=255)
                                                    aGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCg                                                                                              >  1:3172936/1‑150 (MQ=255)
                                                    aGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCg                                                                                              >  2:2157451/1‑150 (MQ=255)
                                                    aGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCGGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCTTTTGCCAGCGGCAAAGAAACCATGAAACTCGGCg                                                                                              <  1:1006954/150‑1 (MQ=255)
                                                         gCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctg                                                                                         <  1:502491/150‑1 (MQ=255)
                                                               ctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGaa                                                                                   >  2:1120357/1‑150 (MQ=255)
                                                               ctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGaa                                                                                   >  1:656820/1‑150 (MQ=255)
                                                                 cGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGc                                                                                 >  1:3451371/1‑150 (MQ=255)
                                                                 cGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGc                                                                                 <  1:4226482/150‑1 (MQ=255)
                                                                  gATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGcc                                                                                >  1:672457/1‑150 (MQ=255)
                                                                    tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCt                                                                              >  1:3466536/1‑150 (MQ=255)
                                                                       gCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCaa                                                                           >  1:3212069/1‑150 (MQ=255)
                                                                        cTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAg                                                                          <  2:2721439/150‑1 (MQ=255)
                                                                          gggACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCa                                                                        >  2:484559/1‑150 (MQ=255)
                                                                             aCTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAc                                                                     >  2:3909607/1‑150 (MQ=255)
                                                                             aCTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAc                                                                     >  1:4639779/1‑150 (MQ=255)
                                                                                  ggCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACt                                                                <  2:652672/150‑1 (MQ=255)
                                                                                      gcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCgg                                                            >  1:3203/1‑150 (MQ=255)
                                                                                       caAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGc                                                           <  1:3749030/150‑1 (MQ=255)
                                                                                       caAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGc                                                           >  1:2146305/1‑150 (MQ=255)
                                                                                         aGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGccc                                                         >  2:891139/1‑150 (MQ=255)
                                                                                            tAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGAt                                                      <  1:949860/150‑1 (MQ=255)
                                                                                              aaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTg                                                    >  2:4060864/1‑150 (MQ=255)
                                                                                               aGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTgg                                                   >  2:1809500/1‑150 (MQ=255)
                                                                                                gTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                  >  2:2773509/1‑150 (MQ=255)
                                                                                                gTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                  <  1:1507870/150‑1 (MQ=255)
                                                                                                 tGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                 >  1:1946883/1‑150 (MQ=255)
                                                                                                      gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                            <  2:1940805/150‑1 (MQ=255)
                                                                                                      gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                            <  2:3952835/150‑1 (MQ=255)
                                                                                                      gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                            >  2:1333006/1‑150 (MQ=255)
                                                                                                       aTCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCACAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGt                                           >  2:4655823/1‑150 (MQ=255)
                                                                                                       aTCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGt                                           >  2:3223470/1‑150 (MQ=255)
                                                                                                       aTCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGt                                           >  2:3609620/1‑150 (MQ=255)
                                                                                                        tCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGta                                          >  1:2677085/1‑150 (MQ=255)
                                                                                                         cTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCAGAGtat                                         <  1:2832545/150‑1 (MQ=255)
                                                                                                          tGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                        <  1:4475691/150‑1 (MQ=255)
                                                                                                          tGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                        <  2:3992345/150‑1 (MQ=255)
                                                                                                           ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                       >  1:1527011/1‑150 (MQ=255)
                                                                                                           ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                       >  2:2270984/1‑150 (MQ=255)
                                                                                                           ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                       <  1:4425310/150‑1 (MQ=255)
                                                                                                           ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                       <  1:3830026/150‑1 (MQ=255)
                                                                                                           ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                       >  1:2986001/1‑150 (MQ=255)
                                                                                                             aTCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGc                                     >  1:3280342/1‑150 (MQ=255)
                                                                                                             aTCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGc                                     <  1:1848958/150‑1 (MQ=255)
                                                                                                               ccGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcac                                   <  1:1702096/150‑1 (MQ=255)
                                                                                                                cGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                  >  1:2299107/1‑150 (MQ=255)
                                                                                                                 gTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAt                                 >  1:3831325/1‑150 (MQ=255)
                                                                                                                 gTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAt                                 >  1:2319539/1‑150 (MQ=255)
                                                                                                                 gTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAt                                 >  1:845347/1‑150 (MQ=255)
                                                                                                                     ttCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAcc                             >  2:1001710/1‑150 (MQ=255)
                                                                                                                      tCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCa                            <  2:4623656/150‑1 (MQ=255)
                                                                                                                      tCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCa                            >  1:4199893/1‑150 (MQ=255)
                                                                                                                       ccACGATCTGACCGAAGCAGAGTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGCAGCCCTCAAGCAAACCTACTGCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAg                           >  1:3473799/1‑150 (MQ=255)
                                                                                                                       ccACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAg                           <  1:4674199/150‑1 (MQ=255)
                                                                                                                           gATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAcc                       >  1:2044897/1‑150 (MQ=255)
                                                                                                                            aTCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCg                      >  2:514259/1‑150 (MQ=255)
                                                                                                                             tCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCga                     <  1:2205762/150‑1 (MQ=255)
                                                                                                                                  ccGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTCTATGCACATTACCAGCACCGAAGaaa                >  2:96487/1‑150 (MQ=255)
                                                                                                                                  ccGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaa                >  2:2819994/1‑150 (MQ=255)
                                                                                                                                  ccGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaa                >  2:2209806/1‑150 (MQ=255)
                                                                                                                                   cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa               <  1:2795540/150‑1 (MQ=255)
                                                                                                                                   cGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa               <  2:4739742/150‑1 (MQ=255)
                                                                                                                                     aaGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc             <  2:3018205/150‑1 (MQ=255)
                                                                                                                                     aaGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc             >  1:3557116/1‑150 (MQ=255)
                                                                                                                                     aaGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc             <  1:155155/150‑1 (MQ=255)
                                                                                                                                     aaGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc             >  1:1102095/1‑150 (MQ=255)
                                                                                                                                      aGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACg            <  2:2377997/150‑1 (MQ=255)
                                                                                                                                      aGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACg            <  2:2359918/150‑1 (MQ=255)
                                                                                                                                         aGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg         >  2:1249879/1‑150 (MQ=255)
                                                                                                                                           aCTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGa       >  2:4186135/1‑150 (MQ=255)
                                                                                                                                             ttCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATc     <  1:3065849/150‑1 (MQ=255)
                                                                                                                                               ccAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCa   >  1:3134732/1‑150 (MQ=255)
                                                                                                                                                cAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCaa  >  1:4401152/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAA  >  CP009273/754423‑754716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑