BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	853,968	A→T	8.3%	Y184N (TAC→AAC)	ybiU ←	DUF1479 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	853,968	0	A	T	8.3%	936.1 / 53.7	293	Y184N (TAC→AAC)	ybiU	DUF1479 family protein
Reads supporting (aligned to +/- strand): ref base A (121/144); new base T (7/19); total (130/163)
Fisher's exact test for biased strand distribution p-value = 9.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGAC  >  CP009273/853820‑854117

    atTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGa                                                                                                                                                   <  1:3302130/150‑1 (MQ=255)

     ttGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGAc                                                                                                                                                  >  1:806343/1‑150 (MQ=255)

          ccAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTca                                                                                                                                             <  2:2714800/150‑1 (MQ=255)

           cAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTcac                                                                                                                                            <  2:950983/150‑1 (MQ=255)

           cAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCCCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTcac                                                                                                                                            <  2:2894110/150‑1 (MQ=255)

            aGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTcaca                                                                                                                                           >  1:4139876/1‑150 (MQ=255)

             gATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTTGATGACGCTCACAt                                                                                                                                          >  1:411705/1‑150 (MQ=255)

             gATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACAt                                                                                                                                          <  1:769695/150‑1 (MQ=255)

             gATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACAt                                                                                                                                          <  1:910557/150‑1 (MQ=255)

               ttGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATcc                                                                                                                                        >  1:523682/1‑150 (MQ=255)

                tGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCg                                                                                                                                       <  1:1916340/150‑1 (MQ=255)

                tGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCg                                                                                                                                       <  1:1946387/150‑1 (MQ=255)

                tGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCg                                                                                                                                       <  2:1762402/150‑1 (MQ=255)

                 gCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCgg                                                                                                                                      >  2:3859550/1‑150 (MQ=255)

                  ccATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCggg                                                                                                                                     <  1:3311197/150‑1 (MQ=255)

                   cATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTG‑CCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGtt                                                                                                                                   <  1:3447812/150‑1 (MQ=255)

                    aTTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGtt                                                                                                                                   <  1:3941705/150‑1 (MQ=255)

                    aTTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGtt                                                                                                                                   >  1:2274914/1‑150 (MQ=255)

                     ttAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTa                                                                                                                                  <  2:2789224/150‑1 (MQ=255)

                      tAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTTGATGACGCTCACATCCGGGTTaa                                                                                                                                 <  2:2572885/150‑1 (MQ=255)

                       aaaGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTaaa                                                                                                                                <  1:3063608/150‑1 (MQ=255)

                       aaaGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTaaa                                                                                                                                <  1:1514497/150‑1 (MQ=255)

                           aCGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCAt                                                                                                                            <  2:2722020/150‑1 (MQ=255)

                            cGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCAtt                                                                                                                           <  2:4657262/150‑1 (MQ=255)

                             gTTGGCGAAAACTCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTTGATGACGCTCACATCCGGGTTAAACCATTg                                                                                                                          >  1:3263361/1‑150 (MQ=255)

                             gTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTg                                                                                                                          >  2:439740/1‑150 (MQ=255)

                               tGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCt                                                                                                                        <  1:3374170/150‑1 (MQ=255)

                                 gCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCttt                                                                                                                      <  1:4156133/150‑1 (MQ=255)

                                  cGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTTGATGACGCTCACATCCGGGTTAAACCATTGCTTTc                                                                                                                     <  1:3652236/150‑1 (MQ=255)

                                  cGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTc                                                                                                                     <  1:4235895/150‑1 (MQ=255)

                                  cGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTc                                                                                                                     <  1:1036355/150‑1 (MQ=255)

                                  cGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTGTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTc                                                                                                                     >  2:3124495/1‑150 (MQ=255)

                                   gAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTTGATGACGCTCACATCCGGGTTAAACCATTGCTTTcc                                                                                                                    >  1:1373262/1‑150 (MQ=255)

                                   gAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTTGATGACGCTCACATCCGGGTTAAACCATTGCTTTcc                                                                                                                    >  1:2028870/1‑150 (MQ=255)

                                   gAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTcc                                                                                                                    <  1:2584627/150‑1 (MQ=255)

                                    aaaaCGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCa                                                                                                                   <  1:2879900/150‑1 (MQ=255)

                                      aaCGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATc                                                                                                                 >  1:4554022/1‑150 (MQ=255)

                                        cgcgCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCAc                                                                                                               <  2:1288339/150‑1 (MQ=255)

                                         gcgcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACt                                                                                                              <  2:4115835/150‑1 (MQ=255)

                                         gcgcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACt                                                                                                              <  2:1661103/150‑1 (MQ=255)

                                          cgcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACtt                                                                                                             <  2:1923365/150‑1 (MQ=255)

                                          cgcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACtt                                                                                                             >  2:4790173/1‑150 (MQ=255)

                                           gcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACttt                                                                                                            <  2:2663368/150‑1 (MQ=255)

                                           gcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACttt                                                                                                            <  2:705241/150‑1 (MQ=255)

                                             tGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCa                                                                                                          >  2:1534915/1‑150 (MQ=255)

                                               aTACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCaaa                                                                                                        <  2:1442544/150‑1 (MQ=255)

                                               aTACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCaaa                                                                                                        <  1:1666545/150‑1 (MQ=255)

                                                     tGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCca                                                                                                 >  1:4075151/1‑150 (MQ=255)

                                                       gAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCcaca                                                                                               <  2:3815468/150‑1 (MQ=255)

                                                       gAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCcaca                                                                                               >  1:4055279/1‑150 (MQ=255)

                                                        aaGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAg                                                                                              <  2:4262768/150‑1 (MQ=255)

                                                         agcagcCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGa                                                                                             >  1:3528046/1‑150 (MQ=255)

                                                         agcagcCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGa                                                                                             <  2:3416744/150‑1 (MQ=255)

                                                           cagccagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACg                                                                                           <  2:422099/150‑1 (MQ=255)

                                                            agccagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGa                                                                                          <  1:2042135/150‑1 (MQ=255)

                                                            agccagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGa                                                                                          <  1:2390399/150‑1 (MQ=255)

                                                            agccagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGa                                                                                          <  2:3549513/150‑1 (MQ=255)

                                                            agccagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTAGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATAGCTTTCAAACACTTTCAAATG‑TCCACAGACGa                                                                                          >  2:749780/1‑150 (MQ=255)

                                                               cagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTg                                                                                       <  1:1534915/150‑1 (MQ=255)

                                                               cagcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTg                                                                                       >  1:1327611/1‑150 (MQ=255)

                                                                agcGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTga                                                                                      >  1:210722/1‑150 (MQ=255)

                                                                   gTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGaa                                                                                   >  2:1470509/1‑150 (MQ=255)

                                                                   gTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGaa                                                                                   <  1:2812404/150‑1 (MQ=255)

                                                                     tCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAAc                                                                                 >  1:4426327/1‑150 (MQ=255)

                                                                      ccAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACg                                                                                <  1:3865653/150‑1 (MQ=255)

                                                                        aGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGAc                                                                              <  2:3889298/150‑1 (MQ=255)

                                                                          tGCCCCGGCGTCGGTTTGCGCTCCAAGACCTTGGGTGGTGGTTCCGGGCGGAGGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCGGGGTTACACCATTGCTTTCCATCACTTTCAATTG‑TCCACAGACGATTGAGAAACGACTg                                                                            <  1:2331741/150‑1 (MQ=255)

                                                                                                 cAAGACCTTTGGTGGTCGTTCCGGGCGGACGGCGGGGGATACGGTCTGGTTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCActct                                                     <  2:1422708/150‑1 (MQ=255)

                                                                                                  aaGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTg                                                    >  1:3826408/1‑150 (MQ=255)

                                                                                                    gACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGc                                                  >  2:4554598/1‑150 (MQ=255)

                                                                                                    gACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGc                                                  >  2:3375045/1‑150 (MQ=255)

                                                                                                         ttGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGcc                                             >  1:988390/1‑150 (MQ=255)

                                                                                                             aGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCa                                         >  2:52492/1‑150 (MQ=255)

                                                                                                                  gTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTgc                                    >  2:4289482/1‑150 (MQ=255)

                                                                                                                       gggCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTg                               >  1:64715/1‑150 (MQ=255)

                                                                                                                        ggCGGACGGCGGCGGATCCGGTCAGGGTTGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACTTTTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGc                              <  1:3161322/150‑1 (MQ=255)

                                                                                                                             cgggcggcgtATACGGTCTGGTTTTTTTACGCACACATCGGGTTTAAACCTTTGTTTTCAATCACTTTCAAAGG‑ACCACAGCCGATTGATACACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCa                         <  1:1655480/148‑1 (MQ=255)

                                                                                                                              cggcggcggATACGGTCAGGGTTGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAg                        >  2:3730667/1‑150 (MQ=255)

                                                                                                                              cggcggcggATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAg                        <  2:4865409/150‑1 (MQ=255)

                                                                                                                                gcggcgtATACGGTCTGGTTATTTGACGCTCACATCCGGGTTAAACCATGGCTTACCATCACTTTAAAATG‑TCCACCGCCGTTTGAGACACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTa                      <  1:1859879/150‑1 (MQ=255)

                                                                                                                                  ggcggATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGa                    >  1:4803182/1‑150 (MQ=255)

                                                                                                                                     ggATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATgg                 >  2:2107727/1‑150 (MQ=255)

                                                                                                                                      gATACGGTCAGGGTAGATGACGCTCACATCCGGGTCAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATggg                >  1:4643906/1‑150 (MQ=255)

                                                                                                                                       atcgggTCTGGGTTGTTGAGGCACACATCGGGTTAAAACCATGGCTTTCCAACACTTTCAATTG‑TCCACCGACGTTTGATAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATgggg               <  1:2919510/146‑1 (MQ=255)

                                                                                                                                       aTACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATgggg               >  1:2430546/1‑150 (MQ=255)

                                                                                                                                        tACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGt              <  2:4728023/150‑1 (MQ=255)

                                                                                                                                            gTCTGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑ACCACAGACGATTGAGACACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAt          <  2:4150012/150‑1 (MQ=255)

                                                                                                                                            gTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAt          >  2:2268012/1‑150 (MQ=255)

                                                                                                                                            gTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAt          >  1:80111/1‑150 (MQ=255)

                                                                                                                                             tCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAtc         >  2:2977875/1‑150 (MQ=255)

                                                                                                                                             tCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAtc         >  1:1147007/1‑150 (MQ=255)

                                                                                                                                             tCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAtc         >  2:3971694/1‑150 (MQ=255)

                                                                                                                                              ctgggTAGCTTACGCTCACAGCGGTGTTAAACCATTGCTTACCATC‑CTTTACAATGCGCAACGCACTTTTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAtct        <  1:1256142/148‑1 (MQ=255)

                                                                                                                                               aGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAAtctc       >  1:1124460/1‑150 (MQ=255)

                                                                                                                                                gggTAGATGCCGCTCACAACCGGGTAAACCCATTGCTTTCCAACACTTACAAAGG‑TCCACAGACGATTGATAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCg      <  1:4875987/150‑1 (MQ=255)

                                                                                                                                                gggTAGATGACGCTCACATCCGGGTTAAACCTTTGCTTTCCATCACTTTCAAATG‑ACCACAGACGTTTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCg      <  2:1056649/150‑1 (MQ=255)

                                                                                                                                                gggTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCg      >  2:1166792/1‑150 (MQ=255)

                                                                                                                                                   tAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGa   <  2:3212990/150‑1 (MQ=255)

                                                                                                                                                   tAGATCACCCACACAGCGGGGTAAAACAATTGCTTAACATCACTTACAAACG‑CCCACAGACTATTGACACACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGa   <  1:68523/150‑1 (MQ=255)

                                                                                                                                                    aGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGAc  >  2:3629581/1‑150 (MQ=255)

TCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATG‑TCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGAC  >  CP009273/853820‑854117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`