BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,060,633	A→G	31.2%	N381S (AAC→AGC)	yccE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,060,633	0	A	G	31.2%	238.9 / 158.2	169	N381S (AAC→AGC)	yccE	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (47/69); new base G (24/28); total (72/97)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 173 total aligned reads displayed.

GGATAGGATTAAAGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTA  >  CP009273/1060484‑1060782

    aGGATTAAAGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACt                                                                                                                                                   <  2:1613683/150‑1 (MQ=255)

     ggATTAAAGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTg                                                                                                                                                  <  1:2690358/150‑1 (MQ=255)

       aTTAAAGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGc                                                                                                                                                >  2:3997233/1‑150 (MQ=255)

        ttAAAGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCa                                                                                                                                               >  2:420194/1‑150 (MQ=255)

          aaaGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAAt                                                                                                                                             <  2:2143553/150‑1 (MQ=255)

                    agaaTTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACg                                                                                                                                   <  2:2489545/150‑1 (MQ=255)

                    agaaTTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACg                                                                                                                                   <  2:2489544/150‑1 (MQ=255)

                      aaTTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGtt                                                                                                                                 <  2:853980/150‑1 (MQ=255)

                        ttttCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTa                                                                                                                               >  2:2316003/1‑150 (MQ=255)

                         tttCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTAc                                                                                                                              >  1:3248636/1‑150 (MQ=255)

                           tCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAcct                                                                                                                            <  2:3916037/150‑1 (MQ=255)

                           tCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAcct                                                                                                                            <  2:3918177/150‑1 (MQ=255)

                            cTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAcctc                                                                                                                           <  1:4006163/150‑1 (MQ=255)

                             ttgttgTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTAcctcc                                                                                                                          >  1:4166086/1‑150 (MQ=255)

                               gttgttCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAcctccct                                                                                                                        >  2:1144637/1‑150 (MQ=255)

                                ttgttCTTTCAGATAGCATTTGCTCATTATTTTTTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCTACAGATGAAGATCTTGAGTTTGGTTATTTAATGAACACTGGCAATCATTATGACGTTTAcctccctc                                                                                                                       <  1:1435164/150‑1 (MQ=255)

                                   ttCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTg                                                                                                                    <  1:2016791/150‑1 (MQ=255)

                                    tCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGa                                                                                                                   <  2:1895238/150‑1 (MQ=255)

                                    tCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGa                                                                                                                   <  1:432817/150‑1 (MQ=255)

                                       ttCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACt                                                                                                                <  2:2898313/150‑1 (MQ=255)

                                       ttCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACt                                                                                                                <  1:4086447/150‑1 (MQ=255)

                                          aGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACtttt                                                                                                             >  2:4560486/1‑150 (MQ=255)

                                          aGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACtttt                                                                                                             <  2:3444172/150‑1 (MQ=255)

                                             tAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGc                                                                                                          >  2:2666015/1‑150 (MQ=255)

                                             tAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGc                                                                                                          <  2:2291386/150‑1 (MQ=255)

                                                 aTTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAg                                                                                                      >  2:624801/1‑150 (MQ=255)

                                                 aTTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAg                                                                                                      >  2:625044/1‑150 (MQ=255)

                                                  tttGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAgg                                                                                                     <  1:2471325/150‑1 (MQ=255)

                                                    tGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCt                                                                                                   >  2:3043451/1‑150 (MQ=255)

                                                    tGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCt                                                                                                   <  2:211497/150‑1 (MQ=255)

                                                          ttatttatTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAg                                                                                             >  2:40141/1‑150 (MQ=255)

                                                           tatttattGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCt                                                                                                   <  2:4866536/143‑1 (MQ=255)

                                                           tatttattGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCt                                                                                                   >  1:4866536/1‑143 (MQ=255)

                                                           tatttattGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGt                                                                                            <  2:132619/150‑1 (MQ=255)

                                                            atttattGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGtt                                                                                           <  1:1596691/150‑1 (MQ=255)

                                                                attGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAAc                                                                                       <  1:3700992/150‑1 (MQ=255)

                                                                 ttGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACa                                                                                      <  2:3693820/150‑1 (MQ=255)

                                                                   gTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAAt                                                                                    <  1:393437/150‑1 (MQ=255)

                                                                    ttAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATa                                                                                   >  1:2481031/1‑150 (MQ=255)

                                                                     tAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATaa                                                                                  >  2:1962838/1‑150 (MQ=255)

                                                                     tAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATaa                                                                                  >  2:3235374/1‑150 (MQ=255)

                                                                        tAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGa                                                                               <  2:4517043/150‑1 (MQ=255)

                                                                         aGTACTGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGaa                                                                              <  1:3671928/150‑1 (MQ=255)

                                                                         aGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGaa                                                                              <  1:420194/150‑1 (MQ=255)

                                                                         aGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGaa                                                                              >  1:2597845/1‑150 (MQ=255)

                                                                         aGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGaa                                                                              <  2:4463632/150‑1 (MQ=255)

                                                                           tAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAaat                                                                            <  2:1378009/150‑1 (MQ=255)

                                                                           tAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAaat                                                                            <  1:2093250/150‑1 (MQ=255)

                                                                                  gCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATgcg                                                                     <  1:3972752/150‑1 (MQ=255)

                                                                                   cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATgcgc                                                                    >  1:975211/1‑150 (MQ=255)

                                                                                    aTCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCa                                                                   >  1:2291153/1‑150 (MQ=255)

                                                                                       aaTCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACt                                                                <  2:3775207/150‑1 (MQ=255)

                                                                                        aTCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTc                                                               >  1:3089264/1‑150 (MQ=255)

                                                                                           tcCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGAc                                                            <  2:4773537/150‑1 (MQ=255)

                                                                                            ccTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACt                                                           <  1:1300400/150‑1 (MQ=255)

                                                                                               tGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTAtt                                                        >  1:1017399/1‑150 (MQ=255)

                                                                                                 gggAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTa                                                      <  1:1904233/150‑1 (MQ=255)

                                                                                                  ggAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTaa                                                     >  1:1773014/1‑150 (MQ=255)

                                                                                                  ggAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTaa                                                     <  1:2613596/150‑1 (MQ=255)

                                                                                                    aatgaatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAAt                                                   <  1:671804/150‑1 (MQ=255)

                                                                                                     atgaatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATc                                                  <  1:2996817/150‑1 (MQ=255)

                                                                                                      tgaatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCg                                                 >  1:11092/1‑150 (MQ=255)

                                                                                                       taatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATATAAATcct                                                >  2:3131280/2‑148 (MQ=255)

                                                                                                        aatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGtt                                               <  1:3462049/150‑1 (MQ=255)

                                                                                                           ccTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATg                                            >  1:3722956/1‑150 (MQ=255)

                                                                                                            cTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGc                                           <  2:4514641/150‑1 (MQ=255)

                                                                                                            cTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGc                                           >  1:3922822/1‑150 (MQ=255)

                                                                                                                 caccAGATGAAGATCTTGAGTATGGTTAACTACAGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACGTTGTGCTCAGGCTTACAACTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAAttt                                      <  2:4816225/147‑1 (MQ=255)

                                                                                                                 cAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAAttt                                      <  2:428808/150‑1 (MQ=255)

                                                                                                                  aaCAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTa                                     <  1:1790715/150‑1 (MQ=255)

                                                                                                                   aCAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTaa                                    <  1:3349082/150‑1 (MQ=255)

                                                                                                                    cAGATGAAGATCTTGAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAAt                                   >  1:1695985/1‑150 (MQ=255)

                                                                                                                    cAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAAt                                   >  1:4069465/1‑150 (MQ=255)

                                                                                                                    cAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGAAATTTAAt                                   >  2:1281392/1‑150 (MQ=255)

                                                                                                                      gATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATgg                                 <  1:1852792/150‑1 (MQ=255)

                                                                                                                         gAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCaa                              >  2:830244/1‑150 (MQ=255)

                                                                                                                               cTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCat                        <  2:1029215/150‑1 (MQ=255)

                                                                                                                                  gAGTATGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATg                     <  2:3018236/150‑1 (MQ=255)

                                                                                                                                    gTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCACTTTAATGGCAAAGGCATATGCt                   >  1:4087865/1‑150 (MQ=255)

                                                                                                                                     tATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTa                  >  1:3046249/1‑150 (MQ=255)

                                                                                                                                     tATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTa                  <  1:3828703/150‑1 (MQ=255)

                                                                                                                                      aTGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaa                 <  1:2925192/150‑1 (MQ=255)

                                                                                                                                      aTGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaa                 <  1:495304/150‑1 (MQ=255)

                                                                                                                                       tGGTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaa                >  2:115061/1‑150 (MQ=255)

                                                                                                                                       tGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaa                >  2:3607796/1‑150 (MQ=255)

                                                                                                                                        ggTTATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaaa               <  2:3123438/150‑1 (MQ=255)

                                                                                                                                         gTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaaaa              >  1:317180/1‑150 (MQ=255)

                                                                                                                                         gTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaaaa              <  1:3117276/150‑1 (MQ=255)

                                                                                                                                          ttATTTAATGAGCACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAc             >  2:645739/1‑150 (MQ=255)

                                                                                                                                          ttATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAc             >  1:1389824/1‑150 (MQ=255)

                                                                                                                                           tATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAcc            >  1:842314/1‑150 (MQ=255)

                                                                                                                                           tATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAcc            >  2:2692879/1‑150 (MQ=255)

                                                                                                                                           tATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAcc            >  2:261134/1‑150 (MQ=255)

                                                                                                                                             tttAATGAACACTGGCCATCATTATGTAGTTTACCTCCCTCCTGAACATTTTGCTCAGGCTTACCAGTTAAACAATAATGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACGAt          <  1:359183/150‑1 (MQ=255)

                                                                                                                                             tttAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCAt          <  2:1318875/150‑1 (MQ=255)

                                                                                                                                              ttAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCAtt         <  1:944121/150‑1 (MQ=255)

                                                                                                                                                  tGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGtta     >  2:2039659/1‑150 (MQ=255)

                                                                                                                                                   gAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGttat    <  2:470721/150‑1 (MQ=255)

                                                                                                                                                     acacTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGttatta  <  1:4630877/150‑1 (MQ=255)

                                                                                                                                                     acacTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGttatta  <  2:1215631/150‑1 (MQ=255)

GGATAGGATTAAAGCGGTGAAGAATTTTCTTGTTGTTCTTTCAGATAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTA  >  CP009273/1060484‑1060782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`