Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,061,397 G→T 26.9% A119A (GCG→GCT)  agp → glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,061,3970GT26.9% 745.4 / 253.3 333A119A (GCG→GCT) agpglucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base G (120/123);  new base T (34/56);  total (154/179)
Fisher's exact test for biased strand distribution p-value = 6.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

CAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTGGCGGCAATGGA  >  CP009273/1061248‑1061546
                                                                                                                                                     |                                                                                                                                                     
cAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCg                                                                                                                                                       >  2:1130569/1‑150 (MQ=255)
  aCTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTAc                                                                                                                                                     >  2:257569/1‑150 (MQ=255)
    tCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCg                                                                                                                                                   <  1:644263/150‑1 (MQ=255)
    tCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCg                                                                                                                                                   >  1:3670151/1‑150 (MQ=255)
      accaccAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                                                                                                                                 <  1:3977625/150‑1 (MQ=255)
        caccaAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGcaca                                                                                                                                               <  2:2698997/150‑1 (MQ=255)
        caccaAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGcaca                                                                                                                                               >  2:2075057/1‑150 (MQ=255)
           caAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGtt                                                                                                                                            >  2:2921183/1‑150 (MQ=255)
           caAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGtt                                                                                                                                            >  2:2097785/1‑150 (MQ=255)
             aaGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCt                                                                                                                                          >  2:3255166/1‑150 (MQ=255)
              aGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCtt                                                                                                                                         <  1:4563440/150‑1 (MQ=255)
              aGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCtt                                                                                                                                         <  2:46924/150‑1 (MQ=255)
              aGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCtt                                                                                                                                         <  2:3090341/150‑1 (MQ=255)
              aGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCtt                                                                                                                                         <  1:823735/150‑1 (MQ=255)
                gTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTtta                                                                                                                                       <  2:583921/150‑1 (MQ=255)
                gTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTtta                                                                                                                                       >  1:2299358/1‑150 (MQ=255)
                 tGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTttat                                                                                                                                      >  1:683749/1‑150 (MQ=255)
                 tGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTttat                                                                                                                                      >  2:4356848/1‑150 (MQ=255)
                  ggCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTttatt                                                                                                                                     >  2:3799855/1‑150 (MQ=255)
                  ggCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTttatt                                                                                                                                     <  1:1970248/150‑1 (MQ=255)
                    cGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTAc                                                                                                                                   >  2:1311873/1‑150 (MQ=255)
                     gTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTAcc                                                                                                                                  >  2:2050866/1‑150 (MQ=255)
                     gTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTAcc                                                                                                                                  >  2:702307/1‑150 (MQ=255)
                      tGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCg                                                                                                                                 >  1:1501546/1‑150 (MQ=255)
                       gCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCgg                                                                                                                                >  1:1171134/1‑150 (MQ=255)
                             aGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCAtt                                                                                                                          <  2:1717410/150‑1 (MQ=255)
                             aGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCAtt                                                                                                                          <  2:4170462/150‑1 (MQ=255)
                              gtgtATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTc                                                                                                                         >  1:3286130/1‑150 (MQ=255)
                                 tataTGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCg                                                                                                                      <  1:3748545/150‑1 (MQ=255)
                                    atGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCgggg                                                                                                                   <  1:4811064/150‑1 (MQ=255)
                                    atGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCgggg                                                                                                                   <  2:3427080/150‑1 (MQ=255)
                                    atGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCgggg                                                                                                                   <  2:3922906/150‑1 (MQ=255)
                                     tGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGgt                                                                                                                  <  2:1363547/150‑1 (MQ=255)
                                      gggCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGgtg                                                                                                                 >  2:2167156/1‑150 (MQ=255)
                                       ggCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGgtgt                                                                                                                <  2:2431475/150‑1 (MQ=255)
                                       ggCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGgtgt                                                                                                                <  1:2159205/150‑1 (MQ=255)
                                       ggCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGAGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGgtgt                                                                                                                >  1:60756/1‑150 (MQ=255)
                                            ttACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGatat                                                                                                           >  2:374259/1‑150 (MQ=255)
                                            ttACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGatat                                                                                                           >  2:1434100/1‑150 (MQ=255)
                                            ttACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGatat                                                                                                           >  2:3831129/1‑150 (MQ=255)
                                              aCATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTc                                                                                                         >  2:538430/1‑150 (MQ=255)
                                                    gTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGc                                                                                                   <  1:1053892/150‑1 (MQ=255)
                                                    gTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGc                                                                                                   >  2:3539141/1‑150 (MQ=255)
                                                    gTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGc                                                                                                   >  1:599053/1‑150 (MQ=255)
                                                    gTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGc                                                                                                   >  1:3031438/1‑150 (MQ=255)
                                                     tGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCa                                                                                                  <  2:4304381/150‑1 (MQ=255)
                                                     tGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATACCCGGGGTGTGATATTCCTGTGCa                                                                                                  >  1:4017348/1‑150 (MQ=255)
                                                      gAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCAt                                                                                                 <  2:673028/150‑1 (MQ=255)
                                                      gAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCAt                                                                                                 <  2:805666/150‑1 (MQ=255)
                                                       aaTGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATc                                                                                                >  1:2734160/1‑150 (MQ=255)
                                                        aTGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCa                                                                                               >  1:4196124/1‑150 (MQ=255)
                                                        aTGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCa                                                                                               >  1:1870010/1‑150 (MQ=255)
                                                           gctggcAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCa                                                                                            <  2:4001267/150‑1 (MQ=255)
                                                                cAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaa                                                                                       <  2:742477/150‑1 (MQ=255)
                                                                cAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaa                                                                                       >  1:1706355/1‑150 (MQ=255)
                                                                 agagCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaaa                                                                                      >  2:8943/1‑150 (MQ=255)
                                                                  gagCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACTCCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaaaa                                                                                     <  2:4407742/150‑1 (MQ=255)
                                                                  gagCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaaaa                                                                                     <  2:156869/150‑1 (MQ=255)
                                                                  gagCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaaaa                                                                                     <  1:873681/150‑1 (MQ=255)
                                                                   agCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTCCCGGCGCCTTCCCGGGGTGTGATATTCCTGTGCATCACCAGGaaaaaa                                                                                    >  1:335794/1‑150 (MQ=255)
                                                                     cAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATg                                                                                  >  2:4785173/1‑150 (MQ=255)
                                                                      aGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATgg                                                                                 <  2:922858/150‑1 (MQ=255)
                                                                      aGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATgg                                                                                 >  2:2508610/1‑150 (MQ=255)
                                                                       ggggATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATggg                                                                                >  1:2524271/1‑150 (MQ=255)
                                                                       ggggATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATggg                                                                                <  2:4431830/150‑1 (MQ=255)
                                                                       ggggATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATggg                                                                                >  1:507628/1‑150 (MQ=255)
                                                                               tGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATgg                                                                        <  1:4327241/150‑1 (MQ=255)
                                                                               tGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATgg                                                                        >  1:1359547/1‑150 (MQ=255)
                                                                                  aaTCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGAcc                                                                     <  2:4626204/150‑1 (MQ=255)
                                                                                      gggggAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAAc                                                                 >  2:3802615/1‑150 (MQ=255)
                                                                                          gAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCttt                                                             <  1:2446418/150‑1 (MQ=255)
                                                                                                    cgccgTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGa                                                   <  1:3128023/150‑1 (MQ=255)
                                                                                                    cgccgTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGa                                                   <  1:2097778/150‑1 (MQ=255)
                                                                                                       cgTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCCCTATGGACCCAACCTTTAACCCGGTGATCa                                                >  1:2827971/1‑150 (MQ=255)
                                                                                                            acCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgat                                           <  1:1678322/150‑1 (MQ=255)
                                                                                                            acCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgat                                           <  2:1463226/150‑1 (MQ=255)
                                                                                                              cGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgatga                                         <  1:9875/150‑1 (MQ=255)
                                                                                                                  tatgcctatgccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTcc                                     <  1:463565/150‑1 (MQ=255)
                                                                                                                  tatgcctatgccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTcc                                     >  1:4122771/1‑150 (MQ=255)
                                                                                                                  tatgcctatgccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTcc                                     <  2:151936/150‑1 (MQ=255)
                                                                                                                            ccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCa                           <  2:3400423/150‑1 (MQ=255)
                                                                                                                              aaTAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGt                         >  1:25451/1‑150 (MQ=255)
                                                                                                                              aaTAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGt                         >  1:1895929/1‑150 (MQ=255)
                                                                                                                              aaTAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGt                         >  2:2890301/1‑150 (MQ=255)
                                                                                                                              aaTAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGt                         >  2:2674964/1‑150 (MQ=255)
                                                                                                                              aaTAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGt                         >  1:2371025/1‑150 (MQ=255)
                                                                                                                                tAGTCTGCAACGTACCGTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGa                       >  2:2110180/1‑150 (MQ=255)
                                                                                                                                tAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCGACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGa                       >  2:2356182/1‑150 (MQ=255)
                                                                                                                                tAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGa                       >  1:2929742/1‑150 (MQ=255)
                                                                                                                                tAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGa                       <  1:2005571/150‑1 (MQ=255)
                                                                                                                                  gTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAAc                     >  2:4551202/1‑150 (MQ=255)
                                                                                                                                    cTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAg                   >  1:3555880/1‑150 (MQ=255)
                                                                                                                                       cAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCg                >  2:2947575/1‑150 (MQ=255)
                                                                                                                                        aaCGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCgg               <  2:4050899/150‑1 (MQ=255)
                                                                                                                                          cGTACCGGTGCGACCGCACAGTTCTTTATGACCGGCGCATGCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGAGGATTCCGCCGCATTCAGTGAACAGGCGGTg             <  1:2105448/150‑1 (MQ=255)
                                                                                                                                           gTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTgg            <  2:1852812/150‑1 (MQ=255)
                                                                                                                                           gTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTgg            >  1:1006476/1‑150 (MQ=255)
                                                                                                                                             aCCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTggcg          >  1:3804305/1‑150 (MQ=255)
                                                                                                                                                gTTGCTACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTGGCGGCa       <  1:2087045/150‑1 (MQ=255)
                                                                                                                                                 ttGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTGGCGGCaa      <  1:356288/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTCAGTGAACAGGCGGTGGCGGCAATGGA  >  CP009273/1061248‑1061546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑