Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,253,559 G→T 21.3% I137I (ATC→ATA)  pth ← peptidyl‑tRNA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,253,5590GT21.3% 632.2 / 144.0 239I137I (ATC→ATA) pthpeptidyl‑tRNA hydrolase
Reads supporting (aligned to +/- strand):  ref base G (94/94);  new base T (36/15);  total (130/109)
Fisher's exact test for biased strand distribution p-value = 1.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 246 total aligned reads displayed.

CGGTTCGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTC  >  CP009273/1253410‑1253708
                                                                                                                                                     |                                                                                                                                                     
cGGTTCGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCg                                                                                                                                                       <  1:2621039/150‑1 (MQ=255)
cGGTTCGTTGCTTTGGTCAAGCCATCTGTAAACCACATATCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCg                                                                                                                                                       >  1:1745541/1‑150 (MQ=255)
 ggTTCGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGa                                                                                                                                                      >  2:4827835/1‑150 (MQ=255)
   ttCGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGAtt                                                                                                                                                    >  2:3364397/1‑150 (MQ=255)
   ttCGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGAtt                                                                                                                                                    <  1:4503778/150‑1 (MQ=255)
     cGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTcc                                                                                                                                                  >  2:767916/1‑150 (MQ=255)
       ttGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGa                                                                                                                                                >  2:2741189/1‑150 (MQ=255)
       ttGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGa                                                                                                                                                >  2:370350/1‑150 (MQ=255)
       ttGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGa                                                                                                                                                <  2:1915630/150‑1 (MQ=255)
       ttGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGa                                                                                                                                                >  2:2020866/1‑150 (MQ=255)
          cTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGc                                                                                                                                             <  2:679084/150‑1 (MQ=255)
          cTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGc                                                                                                                                             <  1:4660621/150‑1 (MQ=255)
            ttGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGt                                                                                                                                           >  2:1039115/1‑150 (MQ=255)
             tGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTa                                                                                                                                          >  1:3479523/1‑150 (MQ=255)
                  aaGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACgg                                                                                                                                     <  2:4248403/150‑1 (MQ=255)
                     ccATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGa                                                                                                                                  <  2:3304640/150‑1 (MQ=255)
                       aTCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGaaa                                                                                                                                <  1:4303974/150‑1 (MQ=255)
                       aTCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGaaa                                                                                                                                <  1:4082871/150‑1 (MQ=255)
                        tCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAg                                                                                                                               <  2:3499227/150‑1 (MQ=255)
                        tCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAg                                                                                                                               >  2:2723819/1‑150 (MQ=255)
                         cTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACCCAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGt                                                                                                                              <  2:1560199/150‑1 (MQ=255)
                          tGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGtt                                                                                                                             <  1:955441/150‑1 (MQ=255)
                           gTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTa                                                                                                                            >  1:4320576/1‑150 (MQ=255)
                                 cttttttCATTACAACGCGCCGCTTCGTCATTGGCTTCATAAATTAACTTCTGTTCACTAACTGGAGGTTTGCCTAACACAAACCCGACTACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGtta                                                                                                                      <  1:23849/146‑1 (MQ=255)
                                   cctttCAGTACCACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTCACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGttatt                                                                                                                    <  2:3458899/148‑1 (MQ=255)
                                   caTTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGttatt                                                                                                                    <  2:216570/150‑1 (MQ=255)
                                    aTTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGttatta                                                                                                                   <  1:1842634/150‑1 (MQ=255)
                                         aGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCaa                                                                                                              >  1:689394/1‑150 (MQ=255)
                                          gTACACCGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAAt                                                                                                             <  2:3948144/150‑1 (MQ=255)
                                          gTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAAt                                                                                                             <  2:1105351/150‑1 (MQ=255)
                                           tACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAAtt                                                                                                            <  2:3304294/150‑1 (MQ=255)
                                             cAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTa                                                                                                          <  1:437044/150‑1 (MQ=255)
                                              aaCGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTAc                                                                                                         <  2:2927049/150‑1 (MQ=255)
                                              aaCGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTAc                                                                                                         >  1:3995739/1‑150 (MQ=255)
                                                cgcgCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACtg                                                                                                       >  1:1935640/1‑150 (MQ=255)
                                                  cgccgcTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACtgat                                                                                                     <  2:2254774/150‑1 (MQ=255)
                                                   gccgcTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACtgatg                                                                                                    >  2:2451907/1‑150 (MQ=255)
                                                   gccgcTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACtgatg                                                                                                    >  1:4522474/1‑150 (MQ=255)
                                                   gccgcTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACtgatg                                                                                                    >  2:1000019/1‑150 (MQ=255)
                                                     cgcTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTgatgat                                                                                                  >  1:1250994/1‑150 (MQ=255)
                                                       cTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGt                                                                                                <  1:667887/150‑1 (MQ=255)
                                                        ttCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTc                                                                                               >  1:1030125/1‑150 (MQ=255)
                                                          cGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCtt                                                                                             <  1:3241858/150‑1 (MQ=255)
                                                             cAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCa                                                                                          <  1:2523560/150‑1 (MQ=255)
                                                             cAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCa                                                                                          >  2:2668358/1‑150 (MQ=255)
                                                              aaTGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTGGGGTTATTACCCAATTTACTGATGATGTCTTTCAg                                                                                         >  2:3493248/1‑150 (MQ=255)
                                                              aaTGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAg                                                                                         >  1:653871/1‑150 (MQ=255)
                                                                   cTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCAt                                                                                    >  1:662237/1‑150 (MQ=255)
                                                                   cTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCAt                                                                                    >  2:4334321/1‑150 (MQ=255)
                                                                    ttCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCAtt                                                                                   <  2:2334685/150‑1 (MQ=255)
                                                                         caATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGac                                                                              >  2:3725267/1‑150 (MQ=255)
                                                                                cTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATg                                                                       <  2:550595/150‑1 (MQ=255)
                                                                                        cACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGc                                                               >  1:4826319/1‑150 (MQ=255)
                                                                                           tAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCa                                                            >  1:2493100/1‑150 (MQ=255)
                                                                                           tAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCa                                                            >  1:2929132/1‑150 (MQ=255)
                                                                                              cAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAAtt                                                         >  1:4726530/1‑150 (MQ=255)
                                                                                              cAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAAtt                                                         >  1:4407013/1‑150 (MQ=255)
                                                                                                  cGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACACAATTTACTGATGATGTCTTTCAGTCAATAGTGACCACCATGGCCACCGCCCAATTTaaa                                                     >  1:3904855/1‑150 (MQ=255)
                                                                                                   ggTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAAt                                                    >  1:2985205/1‑150 (MQ=255)
                                                                                                    gTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAAtt                                                   >  1:56202/1‑150 (MQ=255)
                                                                                                     tttGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAAttt                                                  <  1:470003/150‑1 (MQ=255)
                                                                                                      ttGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTg                                                 >  2:1293132/1‑150 (MQ=255)
                                                                                                      ttGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTg                                                 <  1:4101996/150‑1 (MQ=255)
                                                                                                       tGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTgg                                                <  1:88654/150‑1 (MQ=255)
                                                                                                        gCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGc                                               <  2:615940/150‑1 (MQ=255)
                                                                                                        gCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGc                                               <  1:1562383/150‑1 (MQ=255)
                                                                                                         ccTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCg                                              <  1:2159747/150‑1 (MQ=255)
                                                                                                             acacAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATGTAAATTTGGCGACGc                                          >  1:1194150/1‑150 (MQ=255)
                                                                                                                caACACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCag                                       <  2:844433/150‑1 (MQ=255)
                                                                                                                  aaaCCGACAACTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCagga                                     >  1:3130691/1‑150 (MQ=255)
                                                                                                                   aaCCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCaggag                                    <  1:4827851/150‑1 (MQ=255)
                                                                                                                     ccGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGc                                  >  1:2632632/1‑150 (MQ=255)
                                                                                                                     ccGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGc                                  >  2:2523241/1‑150 (MQ=255)
                                                                                                                     ccGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGc                                  >  2:4782191/1‑150 (MQ=255)
                                                                                                                       gACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAg                                >  2:922429/1‑150 (MQ=255)
                                                                                                                          aaCTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATc                             >  2:4666536/1‑150 (MQ=255)
                                                                                                                          aaCTTTATTTTTATCGCCCGGATGACCGAGTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATc                             <  1:233747/150‑1 (MQ=255)
                                                                                                                           aCTTTATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATcc                            >  1:943583/1‑150 (MQ=255)
                                                                                                                           aCTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATcc                            >  1:3662213/1‑150 (MQ=255)
                                                                                                                           aCTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATcc                            >  1:3662629/1‑150 (MQ=255)
                                                                                                                             tttATTTTTATCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAg                          >  2:4564080/1‑150 (MQ=255)
                                                                                                                             tttATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAg                          >  1:2037131/1‑150 (MQ=255)
                                                                                                                               tATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGtt                        >  2:236785/1‑150 (MQ=255)
                                                                                                                                 ttggtATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTtcg                      <  2:256942/146‑1 (MQ=255)
                                                                                                                                  ttttATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGTGACGCCAGGAGGCAGATCCAGTtcgt                     >  2:1826764/1‑150 (MQ=255)
                                                                                                                                  ttttATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTtcgt                     >  1:1567726/1‑150 (MQ=255)
                                                                                                                                  ttttATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTtcgt                     >  2:700538/1‑150 (MQ=255)
                                                                                                                                   tttATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTtcgtc                    <  1:273645/150‑1 (MQ=255)
                                                                                                                                    ttATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTtcgtcg                   >  2:2350510/1‑150 (MQ=255)
                                                                                                                                     tATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTcgtcgt                  >  2:868995/1‑150 (MQ=255)
                                                                                                                                      aTCGCCCGGATGACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTcgtcgta                 <  1:1416702/150‑2 (MQ=255)
                                                                                                                                      aTCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTg                 <  1:1320113/150‑1 (MQ=255)
                                                                                                                                          cccGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCAATGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGcc             >  1:380114/1‑150 (MQ=255)
                                                                                                                                             ggATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGccacc          >  2:1384652/1‑150 (MQ=255)
                                                                                                                                             ggATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGccacc          <  2:3265413/150‑1 (MQ=255)
                                                                                                                                              gATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGccacca         >  1:3367772/1‑150 (MQ=255)
                                                                                                                                                 gACCTATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGaa      >  1:3752807/1‑150 (MQ=255)
                                                                                                                                                   ccGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAAtt    >  2:4447988/1‑150 (MQ=255)
                                                                                                                                                    cGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAAttt   <  2:2131373/150‑1 (MQ=255)
                                                                                                                                                     gATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTc  >  2:4420765/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGTTCGTTGCTTTGGTCAAGCCATCTGTAAACCACATTTCAGTACAACGCGCCGCTTCGTCAATGGCTTCATCAATTAACTTCTGTTCACTAACAGGCGGTTTGCCTAACACAAAACCGACAACTTTATTTTTATCGCCCGGATGACCGATTCCGATGCGTAAACGGTGAAAGTTAGGGTTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTC  >  CP009273/1253410‑1253708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑