Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,365,283 G→T 69.6% E271* (GAG→TAG)  ycjM → alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,365,2830GT69.6% 269.4 / 200.1 242E271* (GAG→TAG) ycjMalpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base G (39/34);  new base T (87/71);  total (137/105)
Fisher's exact test for biased strand distribution p-value = 8.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365138‑1365432
                                                                                                                                                 |                                                                                                                                                     
 gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGTTCATTACCTAGAcc                                                                                                                                                  >  1:1793670/1‑150 (MQ=255)
  cTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa                                                                                                                                                 <  1:4495415/150‑1 (MQ=255)
  cTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa                                                                                                                                                 >  2:2851738/1‑150 (MQ=255)
   tGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGGACAGTGATCATTACCGAGACCaa                                                                                                                                                >  1:2083161/1‑150 (MQ=255)
    gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                               <  1:1081125/150‑1 (MQ=255)
    gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                               <  2:719920/150‑1 (MQ=255)
    gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                               <  2:717313/150‑1 (MQ=255)
    gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAAt                                                                                                                                               <  2:1135913/150‑1 (MQ=255)
       tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt                                                                                                                                            <  2:2326628/150‑1 (MQ=255)
       tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACAAATGtt                                                                                                                                            >  2:2173694/1‑150 (MQ=255)
        aTGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCCATGTTc                                                                                                                                           >  1:1660965/1‑150 (MQ=255)
            ccGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTTTAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCa                                                                                                                                       >  1:4846142/1‑150 (MQ=255)
            ccGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTAAACTGATCATTACCTAGACCAATGTTCCGCa                                                                                                                                       >  1:3384628/1‑150 (MQ=255)
            ccGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCa                                                                                                                                       >  1:3380915/1‑150 (MQ=255)
              gCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATa                                                                                                                                     <  1:2943780/150‑1 (MQ=255)
                cTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGTGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaaa                                                                                                                                   >  1:2831057/1‑150 (MQ=255)
                 tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                                  >  2:382894/1‑150 (MQ=255)
                 tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                                  <  1:2384490/150‑1 (MQ=255)
                 tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAg                                                                                                                                  <  1:1387086/150‑1 (MQ=255)
                  ggATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCACGTACAGTGATCATTACCTAGACCAAATTTCCGCATAAAGa                                                                                                                                 >  2:3372479/1‑150 (MQ=255)
                   gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGACCAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTACGCATAAAGac                                                                                                                                >  2:255942/1‑150 (MQ=255)
                   gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTTTAAACTTGCGCCAGGTACAGTGATAATTAACAATAACAATTTTCCGCATAAAGaa                                                                                                                                >  1:3698719/1‑149 (MQ=255)
                   gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac                                                                                                                                <  1:2872797/150‑1 (MQ=255)
                   gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac                                                                                                                                <  1:439349/150‑1 (MQ=255)
                    aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGaca                                                                                                                               <  2:2602502/150‑1 (MQ=255)
                    aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGaca                                                                                                                               <  2:3547021/150‑1 (MQ=255)
                    aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGaca                                                                                                                               >  1:1929021/1‑150 (MQ=255)
                     tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGATCATTAACGAGACCAATGTTCCGCATAAAGacaa                                                                                                                              >  2:1277767/1‑150 (MQ=255)
                     tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaa                                                                                                                              <  1:2471811/150‑1 (MQ=255)
                       ccGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGacaaca                                                                                                                            <  1:1288553/150‑1 (MQ=255)
                         gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACAtt                                                                                                                          >  2:1115216/1‑150 (MQ=255)
                         gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGACAACAtt                                                                                                                          >  2:1631533/1‑150 (MQ=255)
                            ggCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATAATTAACGAGAACAATGTTCCGCATAAAGACAACAATGCt                                                                                                                       >  2:3247276/1‑150 (MQ=255)
                              cTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTa                                                                                                                     <  1:3460211/150‑1 (MQ=255)
                                        aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCa                                                                                                           <  2:1599128/150‑1 (MQ=255)
                                         aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                          <  1:4638804/150‑1 (MQ=255)
                                         aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                          >  2:4277037/1‑150 (MQ=255)
                                               cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATg                                                                                                    <  1:4576327/150‑1 (MQ=255)
                                                 ggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAAAATTGCTTACTTTGGCGCAGGCGATGAc                                                                                                  >  2:13317/1‑150 (MQ=255)
                                                  gAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACg                                                                                                 <  1:4064490/150‑1 (MQ=255)
                                                  gAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACg                                                                                                 <  1:3142624/150‑1 (MQ=255)
                                                          tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACat                                                                                         <  1:2632535/150‑1 (MQ=255)
                                                           gCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata                                                                                        >  2:2647683/1‑150 (MQ=255)
                                                              tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTACGCATAAAGAAAACAATTCTTAATTTGGCGCAGGCGCTGACGAAGCACATATgg                                                                                     >  2:2451905/1‑150 (MQ=255)
                                                               ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgt                                                                                    <  2:2893898/150‑1 (MQ=255)
                                                                catcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtg                                                                                   >  2:3729677/1‑150 (MQ=255)
                                                                 atcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtgt                                                                                  <  2:4620981/150‑1 (MQ=255)
                                                                 atcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgt                                                                                    >  2:1971209/1‑148 (MQ=255)
                                                                     ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCa                                                                              >  2:1648015/1‑150 (MQ=255)
                                                                     ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCa                                                                              <  1:1986202/150‑1 (MQ=255)
                                                                       aaaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                            >  2:2918786/1‑150 (MQ=255)
                                                                           aaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtctc                                                                        <  1:1838526/150‑1 (MQ=255)
                                                                           aaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtctc                                                                        <  1:2451905/150‑1 (MQ=255)
                                                                            acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                       <  1:497691/150‑1 (MQ=255)
                                                                                  cTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACGAGACCAATTTTCCGCATAAATACAACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTATCGCTgccg                                                                 >  1:749313/1‑150 (MQ=255)
                                                                                   tGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTATCGCTtccgc                                                                >  2:735263/1‑150 (MQ=255)
                                                                                   tGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                                >  2:4824349/1‑150 (MQ=255)
                                                                                   tGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                                >  2:2375566/1‑150 (MQ=255)
                                                                                      ttATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCAc                                                             >  2:2130346/1‑150 (MQ=255)
                                                                                        aTCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTACGCAAAAATACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCCCATATTGTGTAACAGTTATCGCTGCCGCCAcag                                                           >  1:1465117/1‑148 (MQ=255)
                                                                                           aaaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtg                                                        <  2:1850811/150‑1 (MQ=255)
                                                                                           aaaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATAATTAACTATACCAATGTTCCGCATAAAGACAACATTGCTTAATTTGGCGCAGGCGATGACGAAGCACATATTGTGTACCAGTTCTCGCTGCCGCCACTGGtg                                                        >  2:1366673/1‑150 (MQ=255)
                                                                                            aaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgc                                                       >  2:560873/1‑150 (MQ=255)
                                                                                              cTGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGATCATTACCGAGAACAAAGTTACGCATAAAGAAAACATTGCTTAATTTTTCGCAGGCGATGACGAAGCACAAATTGTGTAACACTTATAGCTGCCGCCACAGGtgctg                                                     >  2:437248/1‑150 (MQ=255)
                                                                                                gTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCa                                                   <  1:3385143/150‑1 (MQ=255)
                                                                                                gTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCa                                                   <  1:4023347/150‑1 (MQ=255)
                                                                                                  tACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATTACGAAGCACATATGGTGTACCAGTTATCGCTGCCGCCACAGGTGCTGCATg                                                 >  2:4325287/1‑150 (MQ=255)
                                                                                                   aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGc                                                <  1:549684/150‑1 (MQ=255)
                                                                                                      gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                             <  1:1050698/150‑1 (MQ=255)
                                                                                                       tCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTg                                            <  1:328969/150‑1 (MQ=255)
                                                                                                         gATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCa                                          >  1:3857059/1‑150 (MQ=255)
                                                                                                         gATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCa                                          >  1:4638068/1‑150 (MQ=255)
                                                                                                            tattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaa                                       >  1:178614/1‑150 (MQ=255)
                                                                                                             attGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaaa                                      >  2:2283874/1‑150 (MQ=255)
                                                                                                               tGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                                    >  1:1707606/1‑150 (MQ=255)
                                                                                                                gATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                                   <  2:2989837/150‑1 (MQ=255)
                                                                                                                 aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGa                                  >  1:3368780/1‑150 (MQ=255)
                                                                                                                  tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGaa                                 >  2:1705772/1‑150 (MQ=255)
                                                                                                                     cGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                              >  1:1895854/1‑150 (MQ=255)
                                                                                                                      gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                             >  2:1270462/1‑150 (MQ=255)
                                                                                                                        tGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGa                           <  2:3040696/150‑1 (MQ=255)
                                                                                                                        tGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGa                           >  1:1524357/1‑150 (MQ=255)
                                                                                                                              aGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                     <  2:4181545/150‑1 (MQ=255)
                                                                                                                               ggTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt                    <  1:1115216/150‑1 (MQ=255)
                                                                                                                                gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt                   >  2:4844072/1‑150 (MQ=255)
                                                                                                                                gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt                   >  2:3962010/1‑150 (MQ=255)
                                                                                                                                gTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt                   <  2:1660948/150‑1 (MQ=255)
                                                                                                                                  aCAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtgt                 <  1:2173694/150‑1 (MQ=255)
                                                                                                                                     gTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg              >  1:2518251/1‑150 (MQ=255)
                                                                                                                                      tGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGt             >  1:3903135/1‑150 (MQ=255)
                                                                                                                                        aTCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg           >  1:2217927/1‑150 (MQ=255)
                                                                                                                                        aTCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg           <  1:928999/150‑1 (MQ=255)
                                                                                                                                         tCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTggg          >  2:4671033/1‑150 (MQ=255)
                                                                                                                                         tCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTggg          >  1:671070/1‑150 (MQ=255)
                                                                                                                                          cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc         >  2:3574273/1‑150 (MQ=255)
                                                                                                                                           aTTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg        >  1:891399/1‑150 (MQ=255)
                                                                                                                                                 gagaCCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAc  >  2:2674855/1‑150 (MQ=255)
                                                                                                                                                 gagaCCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAc  >  1:4250721/1‑150 (MQ=255)
                                                                                                                                                 gagaCCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAc  >  1:1151412/1‑150 (MQ=255)
                                                                                                                                                 gagaCCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAc  >  1:3609245/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365138‑1365432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑