Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,620,961 T→G 64.5% Q226P (CAA→CCA)  dcp ← dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,620,9610TG64.5% 304.3 / 205.7 199Q226P (CAA→CCA) dcpdipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base T (39/32);  new base G (79/49);  total (118/81)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

GTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAG  >  CP009273/1620812‑1621109
                                                                                                                                                     |                                                                                                                                                    
gtagtGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGgttgtt                                                                                                                                                      <  1:3557474/150‑1 (MQ=255)
  agtGTTGCCTGGTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTggg                                                                                                                                                    <  2:3372113/150‑1 (MQ=255)
    tgtTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGggt                                                                                                                                                  <  1:3386014/150‑1 (MQ=255)
     gtTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGggtg                                                                                                                                                 >  2:2289538/1‑150 (MQ=255)
     catgacTGTTGTGCACGTATCTCCACCATACGTTGAATGATAGCGCGTGTATCATTGGCATAATTTTTTTCCGATCGCGTACAGCCCGCAATAAACAGTTTTTCACCCCTCGCAAGATCGCTCAATTCGGCAAGCGCAGGATGTTGggtg                                                                                                                                                 >  2:1778322/6‑150 (MQ=255)
      ttGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGggtgg                                                                                                                                                >  1:4764486/1‑150 (MQ=255)
       tGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGggtggt                                                                                                                                               >  1:1100703/1‑150 (MQ=255)
         ccTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTAt                                                                                                                                             <  1:2829678/150‑1 (MQ=255)
          cTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTAtt                                                                                                                                            <  2:446286/150‑1 (MQ=255)
          cTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTAtt                                                                                                                                            <  1:94701/150‑1 (MQ=255)
             ttgtGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTcag                                                                                                                                         <  1:166585/150‑1 (MQ=255)
               gtgCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTcagca                                                                                                                                       >  2:1765059/1‑150 (MQ=255)
                tgCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTcagcag                                                                                                                                      >  1:3976207/1‑150 (MQ=255)
                    cGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGa                                                                                                                                  <  1:800375/150‑1 (MQ=255)
                    cGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCACCGGTTGTTGGGTGGTATTCAGCAGCGGa                                                                                                                                  >  2:2435939/1‑150 (MQ=255)
                     ggATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGGCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGaa                                                                                                                                 <  1:2021349/150‑1 (MQ=255)
                      gATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAAt                                                                                                                                >  2:4708788/1‑150 (MQ=255)
                         ctcCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAg                                                                                                                             >  2:3221464/1‑150 (MQ=255)
                          tcCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGc                                                                                                                            >  2:3538334/1‑150 (MQ=255)
                            caccaGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCa                                                                                                                          >  2:2665525/1‑150 (MQ=255)
                               caGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCAttt                                                                                                                       <  1:1197875/150‑1 (MQ=255)
                                  aCGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGtt                                                                                                                    <  1:1014687/150‑1 (MQ=255)
                                      tGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATcc                                                                                                                >  2:3561013/1‑150 (MQ=255)
                                      tGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCAGTTGTTATcc                                                                                                                >  2:4641685/1‑150 (MQ=255)
                                       gAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCa                                                                                                               >  1:416614/1‑150 (MQ=255)
                                        aaTGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAg                                                                                                              >  1:4869877/1‑150 (MQ=255)
                                         aTGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGa                                                                                                             >  1:1390382/1‑150 (MQ=255)
                                           gATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGAcc                                                                                                           >  2:2212422/1‑150 (MQ=255)
                                           gATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGAcc                                                                                                           <  1:1679847/150‑1 (MQ=255)
                                             tAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGAGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCtt                                                                                                         <  1:1855648/150‑1 (MQ=255)
                                              aGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCttt                                                                                                        <  1:3316260/150‑1 (MQ=255)
                                              aGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCttt                                                                                                        <  2:3846189/150‑1 (MQ=255)
                                                cgcgGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTtct                                                                                                      <  1:3750823/150‑1 (MQ=255)
                                                 gcgGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTtctc                                                                                                     <  2:2650201/150‑1 (MQ=255)
                                                 gcgGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTtctc                                                                                                     <  2:4345630/150‑1 (MQ=255)
                                                  cgGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTcg                                                                                                    >  2:2073366/1‑150 (MQ=255)
                                                    ggTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTcgcg                                                                                                  <  2:1117793/150‑1 (MQ=255)
                                                        tCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAgcc                                                                                              <  2:1151039/150‑1 (MQ=255)
                                                             ggCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCctc                                                                                         >  2:3277630/1‑150 (MQ=255)
                                                               catcatTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTg                                                                                       <  1:695266/150‑1 (MQ=255)
                                                                 tcatTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTgcc                                                                                     >  1:2543094/1‑150 (MQ=255)
                                                                 tcatTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTgcc                                                                                     >  1:2542949/1‑150 (MQ=255)
                                                                  catTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTgccg                                                                                    >  2:4101188/1‑150 (MQ=255)
                                                                    tttttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTTTTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTgccgcc                                                                                  <  2:1563025/150‑1 (MQ=255)
                                                                     ttttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCa                                                                                 <  1:1914696/150‑1 (MQ=255)
                                                                     ttttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCa                                                                                 <  1:1914607/150‑1 (MQ=255)
                                                                       ttttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAgc                                                                               <  1:4833707/150‑1 (MQ=255)
                                                                        tttCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAgcg                                                                              <  2:4231729/150‑1 (MQ=255)
                                                                          tCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCa                                                                            <  1:2289538/150‑1 (MQ=255)
                                                                           ccGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCaa                                                                           >  1:765643/1‑150 (MQ=255)
                                                                            cGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAt                                                                          >  1:1561602/1‑150 (MQ=255)
                                                                              cTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtct                                                                        >  2:1999499/1‑150 (MQ=255)
                                                                               tCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtctc                                                                       >  1:84303/1‑150 (MQ=255)
                                                                               tCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtctc                                                                       >  1:2512448/1‑150 (MQ=255)
                                                                               tCGCGCCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtctc                                                                       >  1:420253/1‑150 (MQ=255)
                                                                               tCGCGCCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAAtctc                                                                       >  1:3646168/1‑150 (MQ=255)
                                                                                   gTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGc                                                                   >  1:3961399/1‑150 (MQ=255)
                                                                                      cAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctca                                                                >  2:2205814/1‑150 (MQ=255)
                                                                                         cccGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGctcactc                                                             >  1:1585944/1‑150 (MQ=255)
                                                                                           cGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCAt                                                           >  2:1379434/1‑150 (MQ=255)
                                                                                           cGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCAt                                                           >  2:2362799/1‑150 (MQ=255)
                                                                                             cAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTc                                                         >  2:1250608/1‑150 (MQ=255)
                                                                                               aTAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCt                                                       >  1:4427799/1‑150 (MQ=255)
                                                                                                tAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTg                                                      >  1:1978298/1‑150 (MQ=255)
                                                                                                tAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTg                                                      >  2:30704/1‑150 (MQ=255)
                                                                                                   aCAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCa                                                   <  1:2980184/150‑1 (MQ=255)
                                                                                                      gTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCt                                                >  1:1233221/1‑150 (MQ=255)
                                                                                                       tttttCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTg                                               <  1:3588751/150‑1 (MQ=255)
                                                                                                        ttttCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTgc                                              >  2:1374537/1‑150 (MQ=255)
                                                                                                         tttCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTgcg                                             <  2:1533102/150‑1 (MQ=255)
                                                                                                          ttCACGCGTCCCACGATCGCGCATTTCGGCAATCGCCGGATGTTGGGGGGTATTCAGCAGCGGAATCAGCCATTTCTTATCCAGACCTATCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCATGCCAGCTgcga                                            >  2:1568170/1‑149 (MQ=255)
                                                                                                           tCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGcgcg                                           <  2:1098274/150‑1 (MQ=255)
                                                                                                           tCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGcgcg                                           >  1:3377765/1‑150 (MQ=255)
                                                                                                           tCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGcgcg                                           >  2:19236/1‑150 (MQ=255)
                                                                                                                cgTCGGACGATCGAGCTTTTCGGCAAGCGCCTGTTGTTGGGTGGTATTCAGCAGCTGAATCTTCCATTTGTTATCCAGTCCTTTCTCGCGAGCCGCCTCTGACGCCAGCGCAATCTGTTGCTCACTCATTCCTGCCAGCTGCGCGATATc                                      <  1:2919910/150‑1 (MQ=255)
                                                                                                                 gTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCg                                     <  2:1083807/150‑1 (MQ=255)
                                                                                                                   cGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGtt                                   <  1:3202898/150‑1 (MQ=255)
                                                                                                                   cGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGtt                                   >  2:275324/1‑150 (MQ=255)
                                                                                                                     cACGATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTca                                 >  2:3754043/1‑150 (MQ=255)
                                                                                                                      aCGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTcac                                >  2:3682591/1‑150 (MQ=255)
                                                                                                                        gATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACaa                              >  1:813116/1‑150 (MQ=255)
                                                                                                                        gATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACaa                              >  1:4085896/1‑150 (MQ=255)
                                                                                                                        gATCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACaa                              >  2:4189803/1‑150 (MQ=255)
                                                                                                                        gATCGCGCATTTCGGCAAGCGCCGGTGGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACaa                              >  1:1742563/1‑150 (MQ=255)
                                                                                                                         aTCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAAc                             >  1:1541608/1‑150 (MQ=255)
                                                                                                                          tCGCGCATTTCGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAAcc                            >  1:225463/1‑150 (MQ=255)
                                                                                                                           cgcgCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCa                           <  2:4592686/150‑1 (MQ=255)
                                                                                                                             cgcATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGa                         <  2:3324428/150‑1 (MQ=255)
                                                                                                                                    cGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCgg                  >  2:4616138/1‑150 (MQ=255)
                                                                                                                                    cGGCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCgg                  >  2:1534646/1‑150 (MQ=255)
                                                                                                                                     ggCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGa                 >  2:3051411/1‑150 (MQ=255)
                                                                                                                                     ggCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGa                 >  2:4653206/1‑150 (MQ=255)
                                                                                                                                     ggCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGa                 <  1:2212422/150‑1 (MQ=255)
                                                                                                                                     ggCAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGa                 >  2:1484839/1‑150 (MQ=255)
                                                                                                                                       cAAGCGCCGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGatt               <  1:2675907/150‑1 (MQ=255)
                                                                                                                                            gcCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGatttattt          <  2:261035/150‑1 (MQ=255)
                                                                                                                                             ccGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtg         <  1:4155325/150‑1 (MQ=255)
                                                                                                                                             ccGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtg         >  1:3180000/1‑150 (MQ=255)
                                                                                                                                              cGGTTGTGGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTtgc        <  1:4524601/150‑1 (MQ=255)
                                                                                                                                                    ttgGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAg  <  2:804505/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTAGTGTTGCCTGTTGTGCACGGATCTCCACCAGACGTTGAATGATAGCGCGGGTATCATTGGCATCATTTTTTTCCGCTCGCGTCCAGCCCGCAATAAACAGTTTTTCACGCGTCGCACGATCGCGCATTTCGGCAAGCGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAG  >  CP009273/1620812‑1621109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑