Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,696,612 A→T 70.5% E41D (GAA→GAT)  add → adenosine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,696,6120AT70.5% 464.2 / 253.8 267E41D (GAA→GAT) addadenosine deaminase
Reads supporting (aligned to +/- strand):  ref base A (49/30);  new base T (110/78);  total (159/108)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TTTTATATCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCAC  >  CP009273/1696463‑1696759
                                                                                                                                                     |                                                                                                                                                   
   tataTCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATaca                                                                                                                                                  >  1:3036954/1‑150 (MQ=255)
   tataTCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATaca                                                                                                                                                  >  1:2546485/1‑150 (MQ=255)
     tatCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACt                                                                                                                                                >  2:4192592/1‑150 (MQ=255)
     tatCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACt                                                                                                                                                >  2:1094258/1‑150 (MQ=255)
      atCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTg                                                                                                                                               <  1:77430/150‑1 (MQ=255)
       tCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGa                                                                                                                                              <  2:675038/150‑1 (MQ=255)
          aGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTc                                                                                                                                           >  2:1910706/1‑150 (MQ=255)
             aaaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTcccc                                                                                                                                        >  2:1630117/1‑150 (MQ=255)
             aaaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTcccc                                                                                                                                        >  2:594893/1‑150 (MQ=255)
             aaaaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTcccc                                                                                                                                        >  1:623910/1‑150 (MQ=255)
               aaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCAc                                                                                                                                      <  1:3602339/150‑1 (MQ=255)
               aaGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCAc                                                                                                                                      >  2:135983/1‑150 (MQ=255)
                agagTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACg                                                                                                                                     >  1:1699225/1‑150 (MQ=255)
                agagTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACg                                                                                                                                     <  1:750454/150‑1 (MQ=255)
                agagTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGGATAATATCTCGCTTCCTGCACAATCCCTGGATAAACTGATTCACCACg                                                                                                                                     >  2:701541/1‑150 (MQ=255)
                    tGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCa                                                                                                                                 <  2:118782/150‑1 (MQ=255)
                    tGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCa                                                                                                                                 <  2:4858659/150‑1 (MQ=255)
                      cGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAgg                                                                                                                               <  2:4802712/150‑1 (MQ=255)
                        aCCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTc                                                                                                                             <  1:3214118/150‑1 (MQ=255)
                        aCCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTc                                                                                                                             <  1:297299/150‑1 (MQ=255)
                         ccATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCa                                                                                                                            >  1:368649/1‑150 (MQ=255)
                         ccATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCa                                                                                                                            <  1:4516244/150‑1 (MQ=255)
                          cATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCAt                                                                                                                           <  1:577508/150‑1 (MQ=255)
                           aTGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCAtt                                                                                                                          <  1:1031726/150‑1 (MQ=255)
                           aTGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCAtt                                                                                                                          <  1:581058/150‑1 (MQ=255)
                           aTGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCAtt                                                                                                                          <  1:4346757/150‑1 (MQ=255)
                           aTGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCAtt                                                                                                                          >  2:4451179/1‑150 (MQ=255)
                            tgattgatACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTg                                                                                                                         <  2:1083255/150‑1 (MQ=255)
                               ttgatACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCa                                                                                                                      >  1:1678127/1‑150 (MQ=255)
                                      caccCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAAcc                                                                                                               >  2:2802014/1‑150 (MQ=255)
                                         ccTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGa                                                                                                            >  1:242321/1‑150 (MQ=255)
                                         ccTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGa                                                                                                            >  1:4842875/1‑150 (MQ=255)
                                         ccTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGa                                                                                                            >  1:453630/1‑150 (MQ=255)
                                           tGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATc                                                                                                          <  1:2224921/150‑1 (MQ=255)
                                            gCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCt                                                                                                         <  1:195977/150‑1 (MQ=255)
                                             ccATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTg                                                                                                        >  1:10033/1‑150 (MQ=255)
                                                ttAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTg                                                                                                     <  2:2796831/150‑1 (MQ=255)
                                                ttAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTg                                                                                                     <  1:930246/150‑1 (MQ=255)
                                                 tAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGa                                                                                                    >  2:1144565/1‑150 (MQ=255)
                                                 tAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGa                                                                                                    <  2:1858521/150‑1 (MQ=255)
                                                 tAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGa                                                                                                    >  1:3126549/1‑150 (MQ=255)
                                                     tGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCtt                                                                                                >  1:1304292/1‑150 (MQ=255)
                                                     tGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCtt                                                                                                <  1:4016333/150‑1 (MQ=255)
                                                     tGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCtt                                                                                                >  2:3636088/1‑150 (MQ=255)
                                                        tatCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCt                                                                                             >  2:739950/1‑150 (MQ=255)
                                                        gatCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCt                                                                                             <  2:2221958/149‑1 (MQ=255)
                                                         atCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTg                                                                                            <  2:3326404/150‑1 (MQ=255)
                                                         atCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTg                                                                                            >  1:1591336/1‑150 (MQ=255)
                                                           ccATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGAc                                                                                          >  1:536952/1‑150 (MQ=255)
                                                            cATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGAcc                                                                                         >  1:285037/1‑150 (MQ=255)
                                                             aTCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCa                                                                                        <  2:1716446/150‑1 (MQ=255)
                                                             aTCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCa                                                                                        <  2:278588/150‑1 (MQ=255)
                                                               cGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCaaa                                                                                      <  1:2880361/150‑1 (MQ=255)
                                                                 ccACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACt                                                                                    >  2:2061563/1‑150 (MQ=255)
                                                                      ttGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACt                                                                               >  1:413433/1‑150 (MQ=255)
                                                                       tGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTg                                                                              >  2:2603969/1‑150 (MQ=255)
                                                                          tGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTgggg                                                                           >  2:155365/1‑150 (MQ=255)
                                                                             cAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGt                                                                        <  2:4646521/150‑1 (MQ=255)
                                                                              aaCATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGtt                                                                       <  1:4435360/150‑1 (MQ=255)
                                                                                 aTTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTaaa                                                                    <  1:4481321/150‑1 (MQ=255)
                                                                                   tCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGt                                                                  <  2:4667674/150‑1 (MQ=255)
                                                                                    cGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGtt                                                                 <  1:1921982/150‑1 (MQ=255)
                                                                                     gTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTtc                                                                >  2:4122728/1‑150 (MQ=255)
                                                                                     gTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTtc                                                                >  1:4593274/1‑150 (MQ=255)
                                                                                       ccccAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTtctc                                                              >  1:3580604/1‑150 (MQ=255)
                                                                                            gACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCctc                                                         <  1:1945851/150‑1 (MQ=255)
                                                                                             aCCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCctct                                                        >  2:1811691/1‑150 (MQ=255)
                                                                                              ccATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCctctc                                                       >  1:4666004/1‑150 (MQ=255)
                                                                                                aTTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCtt                                                     <  1:2065991/150‑1 (MQ=255)
                                                                                                  tCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGa                                                   >  1:3394891/1‑150 (MQ=255)
                                                                                                     tGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGc                                                <  2:1678306/150‑1 (MQ=255)
                                                                                                       aaCTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCt                                              >  2:342999/1‑150 (MQ=255)
                                                                                                        aCTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTg                                             <  2:4488833/150‑1 (MQ=255)
                                                                                                         cTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGt                                            >  2:2383651/1‑150 (MQ=255)
                                                                                                         cTTGGCCGCCAGTATAAGATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGt                                            <  2:2942734/150‑1 (MQ=255)
                                                                                                          ttGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCATGTc                                           >  2:3777745/1‑150 (MQ=255)
                                                                                                           tGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcg                                          >  1:1698408/1‑150 (MQ=255)
                                                                                                            ggCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcgc                                         <  1:4192592/150‑1 (MQ=255)
                                                                                                             gccgccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcgcc                                        <  1:2747473/150‑1 (MQ=255)
                                                                                                              ccgccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTcgccg                                       >  2:2862110/1‑150 (MQ=255)
                                                                                                                gccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCcgcg                                     <  2:1013829/150‑1 (MQ=255)
                                                                                                                gccAGTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCcgcg                                     <  2:3463182/150‑1 (MQ=255)
                                                                                                                    gTATAATATCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGc                                 <  2:4842939/150‑1 (MQ=255)
                                                                                                                     tataATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCa                                <  1:847411/150‑1 (MQ=255)
                                                                                                                      ataataTCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCAt                               >  1:1271206/1‑150 (MQ=255)
                                                                                                                        aataTCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCAttt                             <  1:3899671/150‑1 (MQ=255)
                                                                                                                        aataTCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCAttt                             >  2:4340155/1‑150 (MQ=255)
                                                                                                                         atatCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTg                            <  2:3035135/150‑1 (MQ=255)
                                                                                                                           atCTCGCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGaa                          >  2:2267849/1‑150 (MQ=255)
                                                                                                                                gCTTCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACAt                     <  1:3691749/150‑1 (MQ=255)
                                                                                                                                  ttCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTg                   >  1:4258069/1‑150 (MQ=255)
                                                                                                                                   tCCTGCACAATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGa                  >  2:3534347/1‑150 (MQ=255)
                                                                                                                                       gCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGAt              <  2:3731923/150‑1 (MQ=255)
                                                                                                                                          caATCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCa           >  1:2203401/1‑150 (MQ=255)
                                                                                                                                             tCCCTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGcc        >  2:3915800/1‑150 (MQ=255)
                                                                                                                                             tCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGGGGCATTTGAAAACATTGAAGATGCAGcc        >  1:3677830/1‑150 (MQ=255)
                                                                                                                                               ccTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg      <  2:242321/150‑1 (MQ=255)
                                                                                                                                               ccTGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg      <  2:453630/150‑1 (MQ=255)
                                                                                                                                                 tGGATACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTc    >  1:2562885/1‑150 (MQ=255)
                                                                                                                                                 tGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTc    <  2:623910/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTTTATATCCAGAAAAAGAGTGCGACCATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACTTGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCAC  >  CP009273/1696463‑1696759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑