Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,428 C→A 9.9% V134F (GTC→TTC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,4280CA 9.9% 783.7 / 43.6 216V134F (GTC→TTC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (99/95);  new base A (12/10);  total (111/105)
Fisher's exact test for biased strand distribution p-value = 8.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

GGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTT  >  CP009273/1796281‑1796577
                                                                                                                                                   |                                                                                                                                                     
ggCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcgt                                                                                                                                                     >  2:3620579/1‑150 (MQ=255)
ggCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcgt                                                                                                                                                     >  1:4292940/1‑150 (MQ=255)
 gCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcgtc                                                                                                                                                    <  1:2938123/150‑1 (MQ=255)
  cacaTATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcgtcg                                                                                                                                                   <  1:3092261/150‑1 (MQ=255)
     atatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAg                                                                                                                                                >  2:1891214/1‑150 (MQ=255)
      tatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGt                                                                                                                                               >  1:1463446/1‑150 (MQ=255)
        tCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGttt                                                                                                                                             <  2:1730344/150‑1 (MQ=255)
        tCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGttt                                                                                                                                             >  2:2798785/1‑150 (MQ=255)
         cGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGtttt                                                                                                                                            >  1:12339/1‑150 (MQ=255)
          gACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGttttt                                                                                                                                           >  2:3140215/1‑150 (MQ=255)
           aCATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtc                                                                                                                                          >  1:4090783/1‑150 (MQ=255)
            cATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtct                                                                                                                                         <  2:2648903/150‑1 (MQ=255)
               atTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAg                                                                                                                                      >  1:3959396/1‑150 (MQ=255)
                 tcttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCa                                                                                                                                    >  2:1380555/1‑150 (MQ=255)
                     cATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCt                                                                                                                                >  2:4081632/1‑150 (MQ=255)
                      aTGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTc                                                                                                                               <  1:4210703/150‑1 (MQ=255)
                      aTGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTc                                                                                                                               >  2:558808/1‑150 (MQ=255)
                        ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCAt                                                                                                                             <  1:3476891/150‑1 (MQ=255)
                        ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCAt                                                                                                                             <  2:1250606/150‑1 (MQ=255)
                         gAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATg                                                                                                                            <  1:486017/150‑1 (MQ=255)
                          aaGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGc                                                                                                                           <  1:1331886/150‑1 (MQ=255)
                          aaGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGc                                                                                                                           <  1:1143979/150‑1 (MQ=255)
                           aGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa                                                                                                                          >  2:1842895/1‑150 (MQ=255)
                                aGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc                                                                                                                     >  1:3411455/1‑150 (MQ=255)
                                aGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc                                                                                                                     >  2:3269468/1‑150 (MQ=255)
                                aGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc                                                                                                                     >  2:4613488/1‑150 (MQ=255)
                                aGACCTGGCTTGTCATCATGGGCGAGGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTGTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc                                                                                                                     >  2:1682942/1‑150 (MQ=255)
                                  aCCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCtt                                                                                                                   <  1:792865/150‑1 (MQ=255)
                                     tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                                <  1:810552/150‑1 (MQ=255)
                                     tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                                <  1:1266396/150‑1 (MQ=255)
                                     tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                                >  1:4053860/1‑150 (MQ=255)
                                     tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                                <  2:1168266/150‑1 (MQ=255)
                                        cTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAg                                                                                                             >  2:431591/1‑150 (MQ=255)
                                          tGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTACCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTATCAGCAAGCTCATGAATCAGCTTCTCGAGTg                                                                                                           >  2:3074002/1‑150 (MQ=255)
                                           gTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGc                                                                                                          >  2:1484539/1‑150 (MQ=255)
                                              atcatGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTc                                                                                                       <  2:2030660/150‑1 (MQ=255)
                                                 atGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGAc                                                                                                    >  1:1087192/1‑150 (MQ=255)
                                                   gggCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACAt                                                                                                  >  1:3140893/1‑150 (MQ=255)
                                                   gggCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACAt                                                                                                  <  2:1815872/150‑1 (MQ=255)
                                                    ggCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATc                                                                                                 <  2:4796342/150‑1 (MQ=255)
                                                       gATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTc                                                                                              >  2:1957295/1‑150 (MQ=255)
                                                         tGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCt                                                                                            <  1:4230911/150‑1 (MQ=255)
                                                          gTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTg                                                                                           <  2:2048611/150‑1 (MQ=255)
                                                             ttCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGt                                                                                        >  2:2526614/1‑150 (MQ=255)
                                                                  cAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACg                                                                                   >  1:170956/1‑150 (MQ=255)
                                                                   aaGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGt                                                                                  >  2:2901343/1‑150 (MQ=255)
                                                                    aGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTg                                                                                 <  1:4741042/150‑1 (MQ=255)
                                                                     gAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGc                                                                                <  1:1842164/150‑1 (MQ=255)
                                                                     gAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGc                                                                                >  2:2707027/1‑150 (MQ=255)
                                                                      aaTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCg                                                                               >  1:4139487/1‑150 (MQ=255)
                                                                       aTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCgg                                                                              <  2:4269051/150‑1 (MQ=255)
                                                                           agaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCaa                                                                          <  1:3514017/150‑1 (MQ=255)
                                                                            gaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAg                                                                         <  1:224099/150‑1 (MQ=255)
                                                                            gaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAg                                                                         >  2:2222263/1‑150 (MQ=255)
                                                                            gaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAg                                                                         >  2:2222231/1‑150 (MQ=255)
                                                                             aCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGa                                                                        >  2:1477102/1‑150 (MQ=255)
                                                                             aCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGa                                                                        <  1:3471178/150‑1 (MQ=255)
                                                                               tttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATc                                                                      >  1:1537642/1‑150 (MQ=255)
                                                                                ttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCa                                                                     <  1:870824/150‑1 (MQ=255)
                                                                                ttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCa                                                                     >  2:1086818/1‑150 (MQ=255)
                                                                                ttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCa                                                                     >  2:658660/1‑150 (MQ=255)
                                                                                   tAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcg                                                                  <  1:4425192/150‑1 (MQ=255)
                                                                                     gCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtc                                                                <  2:3314812/150‑1 (MQ=255)
                                                                                     gCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtc                                                                >  1:1635560/1‑150 (MQ=255)
                                                                                       tctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGACGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtcgt                                                              >  1:272001/1‑150 (MQ=255)
                                                                                        ctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGta                                                             <  1:70603/150‑1 (MQ=255)
                                                                                        ctcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGta                                                             <  1:3524940/150‑1 (MQ=255)
                                                                                           cccACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaata                                                          <  1:3266578/150‑1 (MQ=255)
                                                                                             cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa                                                        >  2:4727995/1‑150 (MQ=255)
                                                                                             cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa                                                        <  1:3157839/150‑1 (MQ=255)
                                                                                             cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa                                                        <  1:2940759/150‑1 (MQ=255)
                                                                                                ggTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAc                                                     >  1:2945995/1‑150 (MQ=255)
                                                                                                 gTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAcc                                                    <  2:3994745/150‑1 (MQ=255)
                                                                                                  ttGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCg                                                   <  1:2357036/150‑1 (MQ=255)
                                                                                                    ggCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGtt                                                 <  2:3466025/150‑1 (MQ=255)
                                                                                                    ggCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGtt                                                 <  1:2860303/150‑1 (MQ=255)
                                                                                                      cGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGt                                               >  1:1876497/1‑150 (MQ=255)
                                                                                                       gAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                              >  2:30174/1‑150 (MQ=255)
                                                                                                        aaaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCa                                             >  2:1342333/1‑150 (MQ=255)
                                                                                                          aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAAt                                           <  1:2509142/150‑1 (MQ=255)
                                                                                                          aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAAt                                           <  1:1647804/150‑1 (MQ=255)
                                                                                                          aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAAt                                           <  2:3815927/150‑1 (MQ=255)
                                                                                                            tttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATaa                                         <  2:2945995/150‑1 (MQ=255)
                                                                                                                 cgcgCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCggg                                    <  2:2865909/150‑1 (MQ=255)
                                                                                                                 cgcgCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCggg                                    <  2:649714/150‑1 (MQ=255)
                                                                                                                      ttCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGag                               >  1:1307577/1‑149 (MQ=255)
                                                                                                                      ttCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAt                               <  1:4391100/150‑1 (MQ=255)
                                                                                                                           gCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCa                          >  2:696304/1‑150 (MQ=255)
                                                                                                                           gCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCa                          >  1:1007746/1‑150 (MQ=255)
                                                                                                                             cAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAtt                        >  2:2392420/1‑150 (MQ=255)
                                                                                                                                cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                     >  2:1125394/1‑150 (MQ=255)
                                                                                                                                 tGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTgg                    >  1:3893723/1‑150 (MQ=255)
                                                                                                                                  gACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGt                   <  2:3735867/150‑1 (MQ=255)
                                                                                                                                           cttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCa          >  2:714899/1‑150 (MQ=255)
                                                                                                                                            ttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCaa         >  1:2204606/1‑150 (MQ=255)
                                                                                                                                            ttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCaa         >  2:2450389/1‑150 (MQ=255)
                                                                                                                                               aTGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgt      >  1:4127499/1‑150 (MQ=255)
                                                                                                                                                tGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgtt     <  1:2786242/150‑1 (MQ=255)
                                                                                                                                                  aCGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgttgt   <  1:3545514/150‑1 (MQ=255)
                                                                                                                                                   cgtcgtAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgttgtt  <  1:4613488/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTT  >  CP009273/1796281‑1796577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑