Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,805,969 T→A 15.0% L260Q (CTG→CAG)  ydjN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,805,9690TA15.0% 902.6 / 130.1 298L260Q (CTG→CAG) ydjNputative transporter
Reads supporting (aligned to +/- strand):  ref base T (149/104);  new base A (23/22);  total (172/126)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

GAAAGGTGAACGCGTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTG  >  CP009273/1805820‑1806118
                                                                                                                                                     |                                                                                                                                                     
  aaGGTGAACGCGTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTgg                                                                                                                                                     >  2:3777406/1‑150 (MQ=255)
         aCGCGTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGttt                                                                                                                                              <  2:4293693/150‑1 (MQ=255)
           gcgTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCg                                                                                                                                            <  2:4280508/150‑1 (MQ=255)
             gTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGtt                                                                                                                                          <  1:3166776/150‑1 (MQ=255)
             gTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGtt                                                                                                                                          >  2:2400503/1‑150 (MQ=255)
              tCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGAAGATGACCCCTTACGGCGTTCTGGCTCTAATTACCAAAGTGGTAGCAGGTTCAAACTTGCAAGACAACATCAAACAGATAAGTTACGATg                                                                                                                                         >  2:3571827/1‑150 (MQ=255)
                ttAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTc                                                                                                                                       <  2:3619720/150‑1 (MQ=255)
                 tAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTcg                                                                                                                                      >  2:768030/1‑150 (MQ=255)
                 tAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTcg                                                                                                                                      >  2:4804781/1‑150 (MQ=255)
                 tAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTcg                                                                                                                                      >  2:2121383/1‑150 (MQ=255)
                   gCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTcgcg                                                                                                                                    >  1:3618611/1‑150 (MQ=255)
                    ccGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGt                                                                                                                                   >  2:4448058/1‑150 (MQ=255)
                     cGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTc                                                                                                                                  >  1:1913985/1‑150 (MQ=255)
                      gCTATCGATACCCTACACAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTcc                                                                                                                                 <  1:2830164/150‑1 (MQ=255)
                         aTCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTAc                                                                                                                              >  1:1595752/1‑150 (MQ=255)
                          tCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTAcc                                                                                                                             <  1:602156/150‑1 (MQ=255)
                          tCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTAcc                                                                                                                             >  1:945962/1‑150 (MQ=255)
                           cGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCt                                                                                                                            >  1:3961811/1‑150 (MQ=255)
                           cGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCt                                                                                                                            >  2:2854186/1‑150 (MQ=255)
                            gATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTc                                                                                                                           <  2:549950/150‑1 (MQ=255)
                            gATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTc                                                                                                                           <  2:819810/150‑1 (MQ=255)
                               aCCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGt                                                                                                                        >  1:2334873/1‑150 (MQ=255)
                               aCCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGt                                                                                                                        <  2:2535009/150‑1 (MQ=255)
                                  cTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTctg                                                                                                                     >  2:1058074/1‑150 (MQ=255)
                                     cAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTctgctg                                                                                                                  >  2:3869789/1‑150 (MQ=255)
                                       aaGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGAt                                                                                                                >  2:3979571/1‑150 (MQ=255)
                                        aGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGAtt                                                                                                               >  1:3407865/1‑150 (MQ=255)
                                         gCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTa                                                                                                              <  2:296008/150‑1 (MQ=255)
                                          cTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTAt                                                                                                             >  2:3170830/1‑150 (MQ=255)
                                            gggTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGt                                                                                                           <  1:1713877/150‑1 (MQ=255)
                                              gTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGttt                                                                                                         <  2:2899918/150‑1 (MQ=255)
                                                 atgaAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCa                                                                                                      >  1:587691/1‑150 (MQ=255)
                                                  tgaAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAg                                                                                                     >  1:2409465/1‑150 (MQ=255)
                                                   gaAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGt                                                                                                    >  1:779126/1‑150 (MQ=255)
                                                    aaaCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTg                                                                                                   <  1:1432541/150‑1 (MQ=255)
                                                     gaCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGc                                                                                                  >  2:2078298/2‑150 (MQ=255)
                                                     aaCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGc                                                                                                  >  1:2176478/1‑150 (MQ=255)
                                                     aaCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGc                                                                                                  >  1:523930/1‑150 (MQ=255)
                                                     aaCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGc                                                                                                  >  1:3947992/1‑150 (MQ=255)
                                                     aaCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGc                                                                                                  >  2:2285836/1‑150 (MQ=255)
                                                      aCTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCa                                                                                                 <  2:1344070/150‑1 (MQ=255)
                                                          gTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGc                                                                                             >  1:753910/1‑150 (MQ=255)
                                                          gTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGc                                                                                             <  2:884185/150‑1 (MQ=255)
                                                             cGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGACGTGTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCAtt                                                                                          >  2:2025410/1‑150 (MQ=255)
                                                             cGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCAtt                                                                                          <  1:2437842/150‑1 (MQ=255)
                                                              gCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTc                                                                                         >  2:437094/1‑150 (MQ=255)
                                                              gCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTc                                                                                         >  1:1188831/1‑150 (MQ=255)
                                                              gCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTc                                                                                         >  1:2662725/1‑150 (MQ=255)
                                                              gCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTc                                                                                         >  1:3837319/1‑150 (MQ=255)
                                                                cTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTctg                                                                                       <  1:3806787/150‑1 (MQ=255)
                                                                 tGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTctgc                                                                                      <  1:863846/150‑1 (MQ=255)
                                                                   gTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTctgctg                                                                                    <  1:991335/150‑1 (MQ=255)
                                                                    tCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTgg                                                                                   >  2:3282332/1‑150 (MQ=255)
                                                                     cATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACATCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTggg                                                                                  >  2:3457587/1‑150 (MQ=255)
                                                                      aTGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGc                                                                                 >  1:4434042/1‑150 (MQ=255)
                                                                       tGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCa                                                                                >  2:430941/1‑150 (MQ=255)
                                                                             tGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATg                                                                          >  2:4682686/1‑150 (MQ=255)
                                                                               aCCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGc                                                                        <  1:3062750/150‑1 (MQ=255)
                                                                                ccccTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCg                                                                       >  1:4078566/1‑150 (MQ=255)
                                                                                 cccTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGt                                                                      >  1:255785/1‑150 (MQ=255)
                                                                                   cTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGa                                                                    <  1:4620325/150‑1 (MQ=255)
                                                                                     tACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGt                                                                  >  2:2720128/1‑150 (MQ=255)
                                                                                     tACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGt                                                                  >  1:4156922/1‑150 (MQ=255)
                                                                                      aCGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTc                                                                 >  2:4442700/1‑150 (MQ=255)
                                                                                      aCGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTc                                                                 <  2:2798344/150‑1 (MQ=255)
                                                                                       cGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTcc                                                                <  2:3611530/150‑1 (MQ=255)
                                                                                        ggCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCg                                                               <  1:223313/150‑1 (MQ=255)
                                                                                            ttCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGa                                                           >  1:3531760/1‑150 (MQ=255)
                                                                                             tCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGaa                                                          <  1:3275966/150‑1 (MQ=255)
                                                                                              cTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAg                                                         <  2:4550490/150‑1 (MQ=255)
                                                                                               tGGCTCTAATGACCAACGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGt                                                        <  2:4125466/150‑1 (MQ=255)
                                                                                               tGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGt                                                        >  2:1146531/1‑150 (MQ=255)
                                                                                               tGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGt                                                        >  1:1018749/1‑150 (MQ=255)
                                                                                               tGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGt                                                        <  2:1718729/150‑1 (MQ=255)
                                                                                               tGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGt                                                        <  2:2246863/150‑1 (MQ=255)
                                                                                                ggCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCAGTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTa                                                       <  2:4461672/150‑1 (MQ=255)
                                                                                                   tctAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACtt                                                    <  2:716701/150‑1 (MQ=255)
                                                                                                     tAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTcc                                                  <  2:1897083/150‑1 (MQ=255)
                                                                                                            cAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGt                                           >  1:4705516/1‑150 (MQ=255)
                                                                                                            cAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGt                                           <  1:2137756/150‑1 (MQ=255)
                                                                                                              aaGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTAt                                         <  2:1517356/150‑1 (MQ=255)
                                                                                                              aaGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTAt                                         <  2:4193697/150‑1 (MQ=255)
                                                                                                                gTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATgg                                       >  1:3426424/1‑150 (MQ=255)
                                                                                                                 tGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTCTGGc                                      >  1:4196478/1‑150 (MQ=255)
                                                                                                                 tGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGc                                      >  2:2575930/1‑150 (MQ=255)
                                                                                                                     tGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCtgt                                  <  2:1558407/150‑1 (MQ=255)
                                                                                                                       cAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGc                                >  2:1452262/1‑150 (MQ=255)
                                                                                                                        aGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCt                               <  1:730628/150‑1 (MQ=255)
                                                                                                                          gTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCATGTGCTGa                             >  2:4630145/1‑150 (MQ=255)
                                                                                                                              tAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGtt                         >  2:4215578/1‑150 (MQ=255)
                                                                                                                                aCTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTg                       <  1:2749060/150‑1 (MQ=255)
                                                                                                                                 cTTGCAAGACATCATCAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGc                      >  2:2145807/1‑150 (MQ=255)
                                                                                                                                     cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCttt                  <  1:1852593/150‑1 (MQ=255)
                                                                                                                                       cgACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCGACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCCTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCAGTGCTGACGTTAGCCTTTAc                <  2:2452034/149‑1 (MQ=255)
                                                                                                                                           atcatcAAACAGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc            >  1:3449587/1‑150 (MQ=255)
                                                                                                                                                caAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTc       >  1:1255847/1‑150 (MQ=255)
                                                                                                                                                  aaCTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATTTTTGCAGTGCATGGCATTCTGCTGGGCATTAATTGCGTGAGTCCGCTGAAGTACTTCCGTAATGTATTGCCTGTGCTGACGTTTGACTTTTACAGCCGTTCCa     >  1:3687855/1‑150 (MQ=255)
                                                                                                                                                     tGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtg  >  1:244141/1‑150 (MQ=255)
                                                                                                                                                     tGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtg  >  1:4136904/1‑150 (MQ=255)
                                                                                                                                                     tGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtg  >  1:1681854/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAAGGTGAACGCGTCTTAGCCGCTATCGATACCCTACAAAGCTGGGTGATGAAACTGGTTCGCCTGGTCATGCAGTTGACCCCTTACGGCGTTCTGGCTCTAATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTG  >  CP009273/1805820‑1806118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑