Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,835 A→C 11.0% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8350AC11.0% 682.6 / 4.2 277W53G (TGG→GGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (167/66);  new base C (2/39);  total (169/108)
Fisher's exact test for biased strand distribution p-value = 1.24e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

GATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATA  >  CP009273/1823686‑1823984
                                                                                                                                                     |                                                                                                                                                     
gATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCa                                                                                                                                                       >  1:2962960/1‑150 (MQ=255)
 atcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAg                                                                                                                                                      >  1:740357/1‑150 (MQ=255)
 atcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAg                                                                                                                                                      >  2:304559/1‑150 (MQ=255)
  tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgc                                                                                                                                                     >  1:3526414/1‑150 (MQ=255)
  tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgc                                                                                                                                                     >  2:163252/1‑150 (MQ=255)
   catcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcg                                                                                                                                                    <  2:295648/150‑1 (MQ=255)
   catcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGGTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcg                                                                                                                                                    >  1:3748277/1‑150 (MQ=255)
    atcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcgc                                                                                                                                                   >  1:166487/1‑150 (MQ=255)
      cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                 >  2:810161/1‑150 (MQ=255)
      cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                 >  1:4799744/1‑150 (MQ=255)
        ccGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCAGCGCCCAGCGCGGaa                                                                                                                                               >  2:1000943/1‑150 (MQ=255)
         cGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                                                              >  2:2573234/1‑150 (MQ=255)
          gTCTCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCTATAATCGCGGTTATTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             <  2:1290526/150‑1 (MQ=255)
          gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             >  2:966856/1‑150 (MQ=255)
          gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             >  2:3410584/1‑150 (MQ=255)
             aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                                                          >  2:460528/1‑150 (MQ=255)
              cTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgccg                                                                                                                                         >  1:1107421/1‑150 (MQ=255)
               ttCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgccgc                                                                                                                                        >  1:277917/1‑150 (MQ=255)
                    ttGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACggg                                                                                                                                   >  1:5716/1‑150 (MQ=255)
                     tGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGc                                                                                                                                  >  2:3381194/1‑150 (MQ=255)
                      gCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCt                                                                                                                                 >  1:104378/1‑150 (MQ=255)
                          cTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGGGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGccc                                                                                                                             >  2:1094928/1‑150 (MQ=255)
                           tcccccGCGGGGCTTCCCCGTTACTCGGGCAATAAGCGCGTTTAATGCGGCTTTATTGCTTACCCGCCGGGCGGCACTGCGTGCGACAACGGATTTACGTCCACAAAATGAACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCg                                                                                                                            <  2:2872113/148‑1 (MQ=255)
                            tCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGa                                                                                                                           >  2:786083/1‑150 (MQ=255)
                               cAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACaa                                                                                                                        >  1:503378/1‑150 (MQ=255)
                                 gcgcGGCTTACCCTTTTCTCTGGAAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGAAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGc                                                                                                                      <  2:1129076/150‑1 (MQ=255)
                                  cgcgCCTTACCCGTATCACGGGCAATAATCGCGGTAAATTCGGCTTTATTCCTTTCCCGCAGTGCGGCAAACCGTTCGACACCGCCATGACGTTCAGCAAATCACAGCCGCCCCCCGCGCGTAAACGCCGCACGGGCTGCCCGACAAGcc                                                                                                                     <  1:1992992/150‑1 (MQ=255)
                                   gcgGGTTACCGGGTTCTCGGGCATTACTCGCGGTTATTTGGGCTTTATTGCTTGCCGGCGGTGCGGCAAGGCGTTCGACACGGGCATGACGTTCCGCAAATGAACGCCGCGCCCTGGGCGGACACGCCGCACGGGCTGCCCGACAAGCCt                                                                                                                    <  2:973923/150‑1 (MQ=255)
                                    cgtcTTACCGGTTTCTCTGGAAATAATCGCGTTTATTTCGGATTTATTGTTTTCCAGATGTGGGGAAATGCTTTCGAAAACGCATTTACTTTCAGAAAATTAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTg                                                                                                                   <  2:920613/147‑1 (MQ=255)
                                         tACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGa                                                                                                              >  1:464945/1‑150 (MQ=255)
                                           ccggTTTCTCTGGCAATAATCGCGTTTATTTCGGCTTTTTTGCTTTCCCGCTGTGCGGAAAAGCGTTCTAAAACGGCTTGACGTTCCGCAAATTAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAcc                                                                                                            <  2:596984/147‑1 (MQ=255)
                                            ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                           >  2:940941/1‑150 (MQ=255)
                                             cGTTTCTCTGGCAATAATCGCGTTTATTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACACCGGAATGACGTTCAGAAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTg                                                                                                          <  2:3412161/150‑1 (MQ=255)
                                              gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                                                         >  2:4208750/1‑150 (MQ=255)
                                                  ctctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcgg                                                                                                     >  2:1761805/1‑150 (MQ=255)
                                                    ctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                   >  2:1834468/1‑150 (MQ=255)
                                                    ctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                   >  1:4749225/1‑150 (MQ=255)
                                                    ctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                   >  2:1046572/1‑150 (MQ=255)
                                                      ggCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                 >  2:2272922/1‑150 (MQ=255)
                                                        cAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcgg                                                                                               >  1:1540213/1‑150 (MQ=255)
                                                         aataatCGCGTTTAATTCGGCTTTAGTGCTTTCCACCAGTGCGTCAAAGCGTTCGAAACCGGCATGACTTGCCCCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                                              <  1:2585184/150‑1 (MQ=255)
                                                          ataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACTTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggca                                                                                             <  2:4688102/150‑1 (MQ=255)
                                                           taatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                            >  1:2413298/1‑150 (MQ=255)
                                                             atCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                                                                          >  2:677719/1‑150 (MQ=255)
                                                               cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                        >  2:1498181/1‑150 (MQ=255)
                                                                gcgGTTAATTCGGCTTTATTGCTTTCCCGCAGTGCGGAACAGCGTTCGACAAGGGCTTGACTTTCAGAAAATGAAACCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                                                                       <  2:2030541/150‑1 (MQ=255)
                                                                    ttAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                                                                   >  1:394406/1‑150 (MQ=255)
                                                                    ttAATTCGGCTTTATTGCTTGCCAGCTGGGCGGCAACGCGTTCGAAACCGGCATGACGTTCAGCAAATGAACGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccta                                                                                   <  2:2866996/150‑2 (MQ=255)
                                                                     tactggggCTTTTTGGCTTTCCCGCTGGGCGGCAACGGGTTCGCCCCCGGCTGGCCGTTCAGCAATTGAACGCCGCGCCCCGCGCGGACACGCCGCACGGGCGGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                                                  <  2:2444826/144‑1 (MQ=255)
                                                                     tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                                                  >  1:3286707/1‑150 (MQ=255)
                                                                        ttCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                                                               <  2:3524592/150‑1 (MQ=255)
                                                                         tCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCAt                                                                              >  2:4520631/1‑150 (MQ=255)
                                                                            gtttttttGCTTTCCCGCTGTGCGCCAACGCTTTCGAAACCGGAATTAGTTTAACAAAATCAACGCCGCGCCCCGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                           <  1:2214139/148‑1 (MQ=255)
                                                                            gatttATTGATTTCCAGCATTGCGGAAAACCGTTCGACAACGGAATGACGATCAGCAAATCAAAGCCGCGCCCCGCCCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                           <  1:1889719/148‑1 (MQ=255)
                                                                                tATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATCACACCt                                                                       >  2:3817188/1‑150 (MQ=255)
                                                                                tATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                       >  1:908241/1‑150 (MQ=255)
                                                                                 aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAAGGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTc                                                                      >  2:4009640/1‑150 (MQ=255)
                                                                                  ttGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                                                                     >  2:2061833/1‑150 (MQ=255)
                                                                                   tGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGc                                                                    >  1:1016061/1‑150 (MQ=255)
                                                                                     cTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                                                                  <  2:300540/150‑1 (MQ=255)
                                                                                     cTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                                                                  <  2:4825483/150‑1 (MQ=255)
                                                                                     cTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                                                                  >  1:3010387/1‑150 (MQ=255)
                                                                                      tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                 >  2:2489821/1‑150 (MQ=255)
                                                                                      tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                 >  1:127632/1‑150 (MQ=255)
                                                                                      tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                 >  1:1558195/1‑150 (MQ=255)
                                                                                        tCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                                                               >  2:456648/1‑150 (MQ=255)
                                                                                          cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                                                             >  1:1380221/1‑150 (MQ=255)
                                                                                           agcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                            <  2:1451835/150‑1 (MQ=255)
                                                                                              agTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa                                                         >  1:309292/1‑150 (MQ=255)
                                                                                                tGCGGCAACGGGTTCGACACCGGCATGACGTGCAGCACATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAtt                                                       <  2:3017946/150‑1 (MQ=255)
                                                                                                      aatgCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt                                                 <  2:2217527/147‑1 (MQ=255)
                                                                                                       aaGCTTTCGACAACGGAATGACTTTCAGAAAATTAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                                <  2:2933128/150‑1 (MQ=255)
                                                                                                       aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                                <  2:504498/150‑1 (MQ=255)
                                                                                                        tggtttCGACAGCGGATTGACTTTCAGCAAATTAACGCCGCGCCCAGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTca                                               <  1:82422/146‑1 (MQ=255)
                                                                                                          cGTTCGACATCGGCTTTACTTTAAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                             <  2:2605415/150‑1 (MQ=255)
                                                                                                            ttCGACACCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCTGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAcg                                           <  2:2335293/150‑1 (MQ=255)
                                                                                                               gACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa                                        >  2:1305182/1‑150 (MQ=255)
                                                                                                               gACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa                                        >  1:2182129/1‑150 (MQ=255)
                                                                                                                aCAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                       <  2:4062916/150‑1 (MQ=255)
                                                                                                                  aaCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgc                                     >  1:2406716/1‑150 (MQ=255)
                                                                                                                  aaCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgc                                     >  1:2175586/1‑150 (MQ=255)
                                                                                                                     ggCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGcc                                  >  1:288993/1‑150 (MQ=255)
                                                                                                                          gACGTACAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGc                             <  1:4718016/150‑1 (MQ=255)
                                                                                                                                cAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                       <  1:4314782/150‑1 (MQ=255)
                                                                                                                                cAGAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                       <  1:3161799/150‑1 (MQ=255)
                                                                                                                                  gaaaaTGAACGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATc                     <  2:3295422/148‑1 (MQ=255)
                                                                                                                                   cAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATcc                    >  2:3114697/1‑150 (MQ=255)
                                                                                                                                    aaaTGAACGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   <  2:4646211/150‑1 (MQ=255)
                                                                                                                                    aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   >  1:325059/1‑150 (MQ=255)
                                                                                                                                     actGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                  <  2:3705946/148‑1 (MQ=255)
                                                                                                                                      aTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                 >  2:3569802/1‑150 (MQ=255)
                                                                                                                                        gAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCa               >  2:3461574/1‑150 (MQ=255)
                                                                                                                                            gCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGt           >  1:2361603/1‑150 (MQ=255)
                                                                                                                                              cgcgCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTa         >  2:4778952/1‑150 (MQ=255)
                                                                                                                                                cgcCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAAt       <  1:2088887/150‑1 (MQ=255)
                                                                                                                                                cgcCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCTTCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAAt       <  1:1344452/150‑1 (MQ=255)
                                                                                                                                                 gcCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATc      <  2:3226784/150‑1 (MQ=255)
                                                                                                                                                     aGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATa  >  1:2071004/1‑150 (MQ=255)
                                                                                                                                                     aGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATa  >  1:4523240/1‑150 (MQ=255)
                                                                                                                                                     aGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATa  <  2:4279076/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATA  >  CP009273/1823686‑1823984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑