BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,940,407	C→A	24.7%	E11* (GAA→TAA)	yobI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,940,407	0	C	A	24.7%	277.8 / 80.2	124	E11* (GAA→TAA)	yobI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (39/54); new base A (19/12); total (58/66)
Fisher's exact test for biased strand distribution p-value = 9.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

GATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTT  >  CP009273/1940260‑1940554

gATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAg                                                                                                                                                   >  1:2984875/1‑150 (MQ=255)

gATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAg                                                                                                                                                   >  1:2983945/1‑150 (MQ=255)

 aTAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGt                                                                                                                                                  >  2:2435507/1‑150 (MQ=255)

  tAATGCTGTATGATAAAAAAATGCTGTATAGATATCCAGCGAATTATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGTCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTg                                                                                                                                                 <  2:1319047/150‑1 (MQ=255)

    aTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaa                                                                                                                                               >  1:4623008/1‑150 (MQ=255)

    aTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaa                                                                                                                                               <  2:4626125/150‑1 (MQ=255)

    aTGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaa                                                                                                                                               >  1:4622945/1‑150 (MQ=255)

    aTGCTGTATGATAAAAAAATGCGGGAGAGATATCCAGCGAAGGATGACGAAAACGAGCGAGGTGTCTCGATGAACTGATAAAGGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaa                                                                                                                                               <  2:4602207/150‑1 (MQ=255)

     tGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTgaag                                                                                                                                              <  2:3705999/150‑1 (MQ=255)

       cTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTgaagaa                                                                                                                                            <  1:1953283/150‑1 (MQ=255)

        tGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTCCAGGTTATATTGGAAGCAAATATTTTAATCTTACATATTAAGTGAAGaaa                                                                                                                                           >  2:4324337/1‑150 (MQ=255)

        tGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTgaagaac                                                                                                                                           >  2:4324264/1‑149 (MQ=255)

        tGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGaaa                                                                                                                                           >  2:4325062/1‑150 (MQ=255)

        tGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGaaa                                                                                                                                           >  2:4324595/1‑150 (MQ=255)

         gTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAAt                                                                                                                                          <  1:4398963/150‑1 (MQ=255)

           aTGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGc                                                                                                                                        <  1:1812921/150‑1 (MQ=255)

             gATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGt                                                                                                                                      <  1:2373141/150‑1 (MQ=255)

             gATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGt                                                                                                                                      <  1:2374746/150‑1 (MQ=255)

                aaaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaat                                                                                                                                   >  1:2129327/1‑150 (MQ=255)

                aaaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaat                                                                                                                                   >  1:4671249/1‑150 (MQ=255)

                 aaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaata                                                                                                                                  <  2:3574249/150‑1 (MQ=255)

                 aaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaata                                                                                                                                  >  2:1940457/1‑150 (MQ=255)

                 aaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaata                                                                                                                                  >  2:1940439/1‑150 (MQ=255)

                 aaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaata                                                                                                                                  <  2:1120523/150‑1 (MQ=255)

                 aaaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGtaata                                                                                                                                  <  2:3327298/150‑1 (MQ=255)

                  aaaaaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGtaataa                                                                                                                                 <  2:2544550/150‑1 (MQ=255)

                     aaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATaaaaa                                                                                                                              >  1:2742724/1‑150 (MQ=255)

                     aaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATaaaaa                                                                                                                              >  1:602037/1‑150 (MQ=255)

                     aaTGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATaaaaa                                                                                                                              >  2:1035822/1‑150 (MQ=255)

                          tGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATAc                                                                                                                         >  1:3820897/1‑150 (MQ=255)

                          tGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATAc                                                                                                                         >  1:4228912/1‑150 (MQ=255)

                           ggATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACa                                                                                                                        <  1:1230680/150‑1 (MQ=255)

                             atagataTCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACAtt                                                                                                                      <  1:4851680/150‑1 (MQ=255)

                                     ccAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGcctcc                                                                                                              <  1:2455532/150‑1 (MQ=255)

                                      cAGCGACGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACCGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGcctcct                                                                                                             <  1:2321931/150‑1 (MQ=255)

                                      cAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCATATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGcctcct                                                                                                             <  2:4702978/150‑1 (MQ=255)

                                      cAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGcctcct                                                                                                             <  1:72435/150‑1 (MQ=255)

                                      cAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGcctcct                                                                                                             <  2:4703320/150‑1 (MQ=255)

                                       aGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTg                                                                                                            >  1:3023248/1‑150 (MQ=255)

                                           aaGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGaaaa                                                                                                        >  2:3697182/1‑150 (MQ=255)

                                            aGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGaaaaa                                                                                                       <  1:3091467/150‑1 (MQ=255)

                                              gATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAaaat                                                                                                     <  2:2796481/150‑1 (MQ=255)

                                              gATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAaaat                                                                                                     >  2:3730464/1‑150 (MQ=255)

                                               aTGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAaaata                                                                                                    >  2:4432302/1‑150 (MQ=255)

                                                       aaCTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAAtttttt                                                                                            >  1:3391108/1‑150 (MQ=255)

                                                       aaCTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAAtttttt                                                                                            >  1:1298822/1‑150 (MQ=255)

                                                          ttGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATg                                                                                         >  1:3737107/1‑150 (MQ=255)

                                                           tGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGc                                                                                        <  2:2376453/150‑1 (MQ=255)

                                                              gAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTAt                                                                                     >  1:736707/1‑150 (MQ=255)

                                                                ggTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTa                                                                                   >  2:1035983/1‑150 (MQ=255)

                                                                  tgtCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACg                                                                                 >  2:4049474/1‑150 (MQ=255)

                                                                     ctcGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGtat                                                                              <  1:1457105/150‑1 (MQ=255)

                                                                        gATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTatata                                                                           <  1:1149520/150‑2 (MQ=255)

                                                                        gATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTatata                                                                           <  1:515873/150‑2 (MQ=255)

                                                                         aTGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATAttt                                                                          <  2:2154135/150‑1 (MQ=255)

                                                                         aTGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATAttt                                                                          <  2:2154194/150‑1 (MQ=255)

                                                                             aCTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTAt                                                                      >  2:4491440/1‑150 (MQ=255)

                                                                               tGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCt                                                                    <  1:1969819/150‑1 (MQ=255)

                                                                                 aaaaaTGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTAt                                                                  <  1:2358612/150‑1 (MQ=255)

                                                                                    aaTGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTc                                                               <  2:1285033/150‑1 (MQ=255)

                                                                                           caGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGaa                                                        >  2:4425571/1‑150 (MQ=255)

                                                                                            aGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAAt                                                       <  2:4691644/150‑1 (MQ=255)

                                                                                               ataATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATgtc                                                    >  2:3888663/1‑148 (MQ=255)

                                                                                                    ttATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGt                                               >  2:4713207/1‑150 (MQ=255)

                                                                                                      aTTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGa                                             >  2:4405886/1‑150 (MQ=255)

                                                                                                               gTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAGAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATattaatt                                    <  2:3448688/150‑1 (MQ=255)

                                                                                                               gTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATattaatt                                    >  2:2241376/1‑150 (MQ=255)

                                                                                                                     ttGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCctg                              <  2:1425930/150‑1 (MQ=255)

                                                                                                                     ttGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCctg                              >  1:2825276/1‑150 (MQ=255)

                                                                                                                     ttGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCctg                              >  2:955081/1‑150 (MQ=255)

                                                                                                                     ttGGAAGCAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCctg                              >  2:955469/1‑150 (MQ=255)

                                                                                                                       ggAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCctgct                            <  2:3914675/150‑1 (MQ=255)

                                                                                                                         aaGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGa                          >  2:1034936/1‑150 (MQ=255)

                                                                                                                            cAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAata                       <  1:1060037/150‑1 (MQ=255)

                                                                                                                            cAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAata                       <  1:4040237/150‑1 (MQ=255)

                                                                                                                            cAAATATTTTAATATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAata                       <  1:4041044/150‑1 (MQ=255)

                                                                                                                               atatTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGa                    <  1:3299930/150‑1 (MQ=255)

                                                                                                                               atatTATAACATTACATATTCAGTGAAGAAAGGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGa                    <  2:2556006/150‑1 (MQ=255)

                                                                                                                                   tttAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAAtt                >  1:3444768/1‑150 (MQ=255)

                                                                                                                                     tAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTgg              >  2:4026612/1‑150 (MQ=255)

                                                                                                                                      aaTATTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGga             <  2:993837/150‑1 (MQ=255)

                                                                                                                                       atatTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGgag            <  1:4147292/150‑1 (MQ=255)

                                                                                                                                       atatTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGgag            <  1:4147255/150‑1 (MQ=255)

                                                                                                                                       atatTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGgag            <  1:4147184/150‑1 (MQ=255)

                                                                                                                                         atTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGat          <  2:2056236/150‑1 (MQ=255)

                                                                                                                                         atTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGat          >  2:4187910/1‑150 (MQ=255)

                                                                                                                                         atTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGat          >  2:1733008/1‑150 (MQ=255)

                                                                                                                                         atTACATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGat          <  1:2435507/150‑1 (MQ=255)

                                                                                                                                          ttACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGata         >  1:1287074/1‑150 (MQ=255)

                                                                                                                                            aCATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATc       <  1:1935629/150‑1 (MQ=255)

                                                                                                                                            aCATATTAAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATc       <  2:928285/150‑1 (MQ=255)

                                                                                                                                             cATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCg      <  2:4622945/150‑1 (MQ=255)

                                                                                                                                             cATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCg      <  2:4623008/150‑1 (MQ=255)

                                                                                                                                              atatTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGt     <  1:1800020/150‑1 (MQ=255)

                                                                                                                                              atatTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGt     <  1:3252608/150‑1 (MQ=255)

                                                                                                                                               tatTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTc    >  1:3165883/1‑150 (MQ=255)

                                                                                                                                                 ttCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCtt  <  2:2983945/150‑1 (MQ=255)

                                                                                                                                                 ttCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCtt  >  1:2190601/1‑150 (MQ=255)

                                                                                                                                                 ttCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCtt  <  2:139226/150‑1 (MQ=255)

                                                                                                                                                 ttCAGAGAAGAAATTCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCtt  <  2:2984875/150‑1 (MQ=255)

GATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCCTGCTGAATATGAAATTGGAGATATCGTCTT  >  CP009273/1940260‑1940554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`