BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,048,571	A→T	12.5%	P10P (CCA→CCT)	amn →	AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,048,571	0	A	T	12.5%	669.4 / 81.9	225	P10P (CCA→CCT)	amn	AMP nucleosidase
Reads supporting (aligned to +/- strand): ref base A (113/83); new base T (21/7); total (134/91)
Fisher's exact test for biased strand distribution p-value = 9.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 229 total aligned reads displayed.

GACAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCACTGACCGTAAC  >  CP009273/2048423‑2048720

   aGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTc                                                                                                                                                    >  2:3426814/1‑150 (MQ=255)

         cgcgCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGACCAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGctc                                                                                                                                              >  2:3220791/1‑148 (MQ=255)

              ttGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGAt                                                                                                                                         <  2:3322086/150‑1 (MQ=255)

               tGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATa                                                                                                                                        >  2:3522006/1‑150 (MQ=255)

                 aTAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTTTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACAGGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCCCTGGATaaa                                                                                                                                      >  2:3389599/1‑150 (MQ=255)

                  tAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAAc                                                                                                                                     <  2:77692/150‑1 (MQ=255)

                  tAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAAc                                                                                                                                     <  1:2316075/150‑1 (MQ=255)

                   aGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACt                                                                                                                                    <  2:962378/150‑1 (MQ=255)

                       tGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGAc                                                                                                                                <  2:4331583/150‑1 (MQ=255)

                            aaGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCt                                                                                                                           >  2:4423352/1‑150 (MQ=255)

                             aGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTg                                                                                                                          <  1:4483313/150‑1 (MQ=255)

                              gATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGt                                                                                                                         >  1:1369227/1‑150 (MQ=255)

                              gATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGt                                                                                                                         <  2:3154665/150‑1 (MQ=255)

                              gATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGt                                                                                                                         <  1:196773/150‑1 (MQ=255)

                              gATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGt                                                                                                                         >  2:1350028/1‑150 (MQ=255)

                              gATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGt                                                                                                                         <  1:3790782/150‑1 (MQ=255)

                                tcatcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTAt                                                                                                                       <  1:1606490/150‑1 (MQ=255)

                                 catcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATg                                                                                                                      >  1:2500948/1‑150 (MQ=255)

                                 catcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATg                                                                                                                      >  1:2501241/1‑150 (MQ=255)

                                  atcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCGGCTCAGGCACTGGATAAACTCGACGCGCTGTATGa                                                                                                                     <  2:2775172/150‑1 (MQ=255)

                                   tcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAg                                                                                                                    >  2:815635/1‑150 (MQ=255)

                                   tcCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAg                                                                                                                    >  1:1730556/1‑150 (MQ=255)

                                    ccGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGc                                                                                                                   >  2:2960708/1‑150 (MQ=255)

                                    ccGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGc                                                                                                                   >  2:1973938/1‑150 (MQ=255)

                                    ccGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGc                                                                                                                   >  2:4858456/1‑150 (MQ=255)

                                         cTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCt                                                                                                              >  1:1894863/1‑150 (MQ=255)

                                            cGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTa                                                                                                           >  1:1835338/1‑150 (MQ=255)

                                             gCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAg                                                                                                          >  2:4613209/1‑150 (MQ=255)

                                             gCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAg                                                                                                          >  1:89903/1‑150 (MQ=255)

                                               cTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTc                                                                                                        >  1:2126571/1‑150 (MQ=255)

                                                   cacTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCAt                                                                                                    >  1:409145/1‑150 (MQ=255)

                                                    acTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCAtt                                                                                                   >  1:3073340/1‑150 (MQ=255)

                                                     cTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTa                                                                                                  <  2:4206285/150‑1 (MQ=255)

                                                      ttttGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTAc                                                                                                 >  2:3610919/1‑150 (MQ=255)

                                                      ttttGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTAc                                                                                                 >  1:599578/1‑150 (MQ=255)

                                                            ttCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACg                                                                                           >  1:4216588/1‑150 (MQ=255)

                                                                 aTTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCAtt                                                                                      >  2:1194355/1‑150 (MQ=255)

                                                                        tgACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACt                                                                               <  1:431646/150‑1 (MQ=255)

                                                                        tgACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACt                                                                               >  2:1676093/1‑150 (MQ=255)

                                                                          aCATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACtat                                                                             <  1:4812526/150‑1 (MQ=255)

                                                                           cATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACtata                                                                            <  2:3221596/150‑1 (MQ=255)

                                                                             tACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATAtt                                                                          >  1:1162438/1‑150 (MQ=255)

                                                                                tATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACa                                                                       <  1:4059570/150‑1 (MQ=255)

                                                                                tATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACa                                                                       <  2:228320/150‑1 (MQ=255)

                                                                                tATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACa                                                                       >  2:2106509/1‑150 (MQ=255)

                                                                                 aTCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACaa                                                                      <  1:3215289/150‑1 (MQ=255)

                                                                                   cGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGt                                                                    <  1:944613/150‑1 (MQ=255)

                                                                                   cGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGt                                                                    <  1:3159758/150‑1 (MQ=255)

                                                                                     gATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑gg                                                                 <  2:1075392/150‑1 (MQ=255)

                                                                                      aTGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGc                                                                >  1:4744322/1‑150 (MQ=255)

                                                                                      aTGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGc                                                                <  1:3445839/150‑1 (MQ=255)

                                                                                      aTGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATAGGCAACTATATTACAAGT‑GGc                                                                >  1:1620029/1‑150 (MQ=255)

                                                                                       tgtgCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCg                                                               <  2:3638958/150‑1 (MQ=255)

                                                                                       tgtgCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCg                                                               >  1:3576237/1‑150 (MQ=255)

                                                                                            ggTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTa                                                          >  1:3130917/1‑150 (MQ=255)

                                                                                             gTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTAc                                                         >  2:1149840/1‑150 (MQ=255)

                                                                                             gTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTAc                                                         >  2:4833482/1‑150 (MQ=255)

                                                                                             gTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTAc                                                         >  2:2388311/1‑150 (MQ=255)

                                                                                              tAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTAcc                                                        <  1:2878363/150‑1 (MQ=255)

                                                                                                aTTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCtg                                                      <  1:2418605/150‑1 (MQ=255)

                                                                                                aTTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGAATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCtg                                                      >  2:1485900/1‑150 (MQ=255)

                                                                                                    tATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCtgatga                                                  >  2:3551982/1‑150 (MQ=255)

                                                                                                      tGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGaaa                                                >  2:634300/1‑150 (MQ=255)

                                                                                                      tGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGaaa                                                >  2:2150702/1‑150 (MQ=255)

                                                                                                       ggAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGaaaa                                               >  2:2588446/1‑150 (MQ=255)

                                                                                                        gAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAAc                                              <  1:3132604/150‑1 (MQ=255)

                                                                                                        gAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAAc                                              >  2:3969218/1‑150 (MQ=255)

                                                                                                        gAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAAc                                              <  1:3409276/150‑1 (MQ=255)

                                                                                                              gagaCACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGATGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGc                                        >  2:1254760/1‑150 (MQ=255)

                                                                                                              gagaCACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGc                                        >  1:140114/1‑150 (MQ=255)

                                                                                                              gagaCACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGc                                        >  1:2569903/1‑150 (MQ=255)

                                                                                                              gagaCACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGc                                        >  1:1254411/1‑150 (MQ=255)

                                                                                                                     acaTTAATATTATGGGCTCGGGTCTGACCCCAGCTCTGGCACTGTTTAAACTCGACGCGCTGTATGAGCATTCTGTAGTCGCTTTACGAAACGCCATTGGCAACTATTTTACATGT‑GGCTAATTACCTTATGAAAACGCCCGCAAACAAg                                 <  2:1648341/150‑1 (MQ=255)

                                                                                                                     acaTGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAg                                 >  1:2311501/1‑150 (MQ=255)

                                                                                                                      cttGAATAATATGGGCTCGGGTCTGACCCCAGCTCTGGCACTGTATAAACTCGACGCGCTGTTTGAGAAATCTTTTGTCGCATTACGCAACGCCATTGGAAACTATATTACAAGT‑GGCTATTTACCTGTTGAAAACGCCCGCAAACAAgg                                <  2:4070582/148‑1 (MQ=255)

                                                                                                                      caTGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAgg                                >  2:666575/1‑150 (MQ=255)

                                                                                                                       aTGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGt                               >  2:1127162/1‑150 (MQ=255)

                                                                                                                          aataatAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGTATAAACTCGACGCGCTTTATGAGAAATGTGTAGTCGAATTACGCAACGCATTTGGAAACTATATTAAAAGT‑GGCGAATTACCTGATTAAAACGCCCGCAAACAAGGTCtt                            <  1:228266/150‑1 (MQ=255)

                                                                                                                             aataaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCttttt                         >  1:3883128/1‑150 (MQ=255)

                                                                                                                              ataaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGTATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTg                        <  2:4290236/150‑1 (MQ=255)

                                                                                                                              ataaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTg                        >  1:119939/1‑150 (MQ=255)

                                                                                                                              ataaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTg                        >  1:462378/1‑150 (MQ=255)

                                                                                                                              ataaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTg                        >  1:661265/1‑150 (MQ=255)

                                                                                                                              ataaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTg                        >  1:2715220/1‑150 (MQ=255)

                                                                                                                               taaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCACCTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGt                       >  2:4206810/1‑150 (MQ=255)

                                                                                                                               taaGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGt                       >  2:4267254/1‑150 (MQ=255)

                                                                                                                                aaGGGCTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTc                      >  1:1482469/1‑150 (MQ=255)

                                                                                                                                 cgggCTCGGGTCTGCCCCCAGCTCAGGCACTGTATAAACTCGACGCGCTTTTTGAGAAATCTTTAGTCGCATTACGCAACGCCTTTGGCAACTTTATTACAAGT‑GGCGAATTACCTGATTAAAACGCCCGCAAACAAGGTCTTTTTGTCt                     <  1:1060038/149‑1 (MQ=255)

                                                                                                                                   ggCTCGGGTCTGACCCCAGCTCTGGCACTGGATAAACTCGCCGCGGTGTAGGAGCAATCTGTCGTCGCATTACGCAACGCCTTGGGAAACTATATTACAAGT‑GGCTATTTACCTGTTCAAAACGCCCGCAAACAAGGTCTTTTTGTCTAt                   <  1:2642653/150‑1 (MQ=255)

                                                                                                                                     cTCCGGTCTGACCCCTGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATcc                 >  2:2207859/1‑150 (MQ=255)

                                                                                                                                      tCCGGTCTGACCCCAGCTCAGGCACTGTATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGAAACTATATTACAAGT‑GGCTAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCa                <  2:3853036/150‑1 (MQ=255)

                                                                                                                                       ccGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGTGGGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCa                >  1:618598/1‑150 (MQ=255)

                                                                                                                                       ccGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCAt               <  2:681664/150‑1 (MQ=255)

                                                                                                                                       ccGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCAt               <  2:2696741/150‑1 (MQ=255)

                                                                                                                                         ggTCTGACCCCATATCAGGCACTGTATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATTTTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCa             <  2:2129221/150‑1 (MQ=255)

                                                                                                                                         ggTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCa             >  2:1958065/1‑150 (MQ=255)

                                                                                                                                         ggTCTGACCCCAGCACAGGCACTTGATAAACTCGACGCGCTTTATGAGCAATCTGTAGTCGCATTACGCAACGCAATTGGCAACTATATTACAAGT‑GGCTAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCa             <  2:4407755/150‑1 (MQ=255)

                                                                                                                                              gACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTCCCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCACTGAc        <  2:4536907/150‑1 (MQ=255)

                                                                                                                                                    aGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCACTGACCGTAAc  >  2:2620696/1‑150 (MQ=255)

                                                                                                                                                    aGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCACTGACCGTAAc  >  2:1896985/1‑150 (MQ=255)

GACAGTCAACGCGCTTGATAGCCTGGCGAAGATCATCCGATCTTCGCCTTACACTTTTGTTTCACATTTCTGTGACATACTATCGGATGTGCGGTAATTGTATGGAACAGGAGACACACATGAATAATAAGGGCTCCGGTCTGACCCCAGCTCAGGCACTGGATAAACTCGACGCGCTGTATGAGCAATCTGTAGTCGCATTACGCAACGCCATTGGCAACTATATTACAAGT‑GGCGAATTACCTGATGAAAACGCCCGCAAACAAGGTCTTTTTGTCTATCCATCACTGACCGTAAC  >  CP009273/2048423‑2048720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`