Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,059,588 C→T 6.8% pseudogene (48/87 nt) yeeH ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,059,5880CT 6.8% 711.0 / 27.9 207pseudogene (48/87 nt)yeeHpseudogene
Reads supporting (aligned to +/- strand):  ref base C (106/87);  new base T (9/5);  total (115/92)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 212 total aligned reads displayed.

CGTCATTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATC  >  CP009273/2059441‑2059737
                                                                                                                                                   |                                                                                                                                                     
cGTCATTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACtt                                                                                                                                                     >  1:4051877/1‑150 (MQ=255)
  tCATTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGt                                                                                                                                                   <  1:4676615/150‑1 (MQ=255)
   cATTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTa                                                                                                                                                  >  1:3147278/1‑150 (MQ=255)
    aTTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTaa                                                                                                                                                 >  1:2672655/1‑150 (MQ=255)
    aTTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTaa                                                                                                                                                 <  2:3973055/150‑1 (MQ=255)
      tGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTaaaa                                                                                                                                               >  2:173754/1‑150 (MQ=255)
      tGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTaaaa                                                                                                                                               >  2:4590837/1‑150 (MQ=255)
       gACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAaac                                                                                                                                              <  1:802260/150‑1 (MQ=255)
        aCAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAaaca                                                                                                                                             >  1:1598271/1‑150 (MQ=255)
         cAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAaacaa                                                                                                                                            >  1:2542400/1‑150 (MQ=255)
          aGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCATGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAaacaac                                                                                                                                           >  1:1249046/1‑150 (MQ=255)
              gTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTaa                                                                                                                                       >  2:2102239/1‑150 (MQ=255)
                ttCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATa                                                                                                                                     >  2:3734902/1‑150 (MQ=255)
                  cTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTACAAGAACTTGTAAAACAACGTAATAGa                                                                                                                                   >  2:218894/1‑150 (MQ=255)
                   tgtgGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAATTTGTAAAACAACGTAATAGaa                                                                                                                                  >  1:2223136/1‑150 (MQ=255)
                    gtgGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATCGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAAt                                                                                                                                 >  2:4618553/1‑150 (MQ=255)
                     tgGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAAtt                                                                                                                                >  2:2236108/1‑150 (MQ=255)
                     tgGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAAtt                                                                                                                                >  2:245875/1‑150 (MQ=255)
                     tgGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAAtt                                                                                                                                >  2:2675599/1‑150 (MQ=255)
                       gATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTAt                                                                                                                              <  1:1831687/150‑1 (MQ=255)
                        aTTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATc                                                                                                                             >  2:2422834/1‑150 (MQ=255)
                          tGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGATTTATCCg                                                                                                                           >  2:4053971/1‑150 (MQ=255)
                          tGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCg                                                                                                                           <  2:3597442/150‑1 (MQ=255)
                           gACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAATTTGTAAAACAACGTAATAGAATTATCCgg                                                                                                                          <  2:2222581/150‑1 (MQ=255)
                            aaacaTTTCATTCAGAAAATTAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGa                                                                                                                         <  2:4400846/148‑1 (MQ=255)
                                 tttCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAAtggt                                                                                                                    >  2:1568259/1‑150 (MQ=255)
                                       tcaGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGAc                                                                                                              <  2:605654/150‑1 (MQ=255)
                                       tcaGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGAc                                                                                                              >  2:3511981/1‑150 (MQ=255)
                                        caGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCAGTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACt                                                                                                             >  2:4373892/1‑150 (MQ=255)
                                          gATAATGAATTAATGCTACTGCCGGAACGACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTAt                                                                                                           >  2:3711885/1‑150 (MQ=255)
                                          gATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTAt                                                                                                           >  2:3711246/1‑150 (MQ=255)
                                              aTGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCAc                                                                                                       >  2:3544669/1‑150 (MQ=255)
                                                gAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTa                                                                                                     >  2:3394096/1‑150 (MQ=255)
                                                 aaTTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAATTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAg                                                                                                    >  1:1463914/1‑150 (MQ=255)
                                                 aaTTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTAGTAAAACAACGTAATAGAAATATACGGAATGGTGGCTACTATGCACTAg                                                                                                    >  1:3289047/1‑150 (MQ=255)
                                                  aTTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCgg                                                                                                                          >  2:4740389/1‑127 (MQ=255)
                                                   ttAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAggg                                                                                                  <  1:3008103/150‑1 (MQ=255)
                                                     aaTGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGaa                                                                                                <  1:4630885/150‑1 (MQ=255)
                                                        gCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGt                                                                                             >  2:788595/1‑150 (MQ=255)
                                                          tACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGc                                                                                           <  1:1372384/150‑1 (MQ=255)
                                                           aCTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCg                                                                                          >  2:6028/1‑150 (MQ=255)
                                                               ccGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATa                                                                                      >  2:4869168/1‑150 (MQ=255)
                                                                cGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATaa                                                                                     <  2:1389718/150‑1 (MQ=255)
                                                                   aacaacCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTg                                                                                  >  1:1000920/1‑150 (MQ=255)
                                                                   aacaacCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTg                                                                                  >  2:3278678/1‑150 (MQ=255)
                                                                   aacaacCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTg                                                                                  >  2:4495378/1‑150 (MQ=255)
                                                                    acaacCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTgg                                                                                 >  1:3741986/1‑150 (MQ=255)
                                                                    acaacCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTgg                                                                                 >  1:1276890/1‑150 (MQ=255)
                                                                      aacCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTgggg                                                                               >  1:4459100/1‑150 (MQ=255)
                                                                              aaCGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAAttctt                                                                       >  2:3378531/1‑150 (MQ=255)
                                                                               aCGGGCTTTGTTTTCTGAAATACAGGCTATTTATTCAACGTAAGCGACAGATCTGCGATGTATAAGAACTTGTAAAACAACGTAATATAATTATCCGGAATGGTGGCGACTATGCACTAGGTAAGGTGCGAATAAGTGGGGAAAttcttc                                                                      <  1:4591036/150‑1 (MQ=255)
                                                                               aCGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAAttcttc                                                                      <  1:173754/150‑1 (MQ=255)
                                                                                cGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATtcttct                                                                     <  1:2725441/150‑1 (MQ=255)
                                                                                cGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATtcttct                                                                     <  1:2486532/150‑1 (MQ=255)
                                                                                 gggCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTtctc                                                                    >  1:4012829/1‑150 (MQ=255)
                                                                                   gCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCgg                                                                  >  2:1922058/1‑150 (MQ=255)
                                                                                   gCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCgg                                                                  <  2:2170246/150‑1 (MQ=255)
                                                                                      ttGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTg                                                               >  2:1276964/1‑150 (MQ=255)
                                                                                       tgtgTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGa                                                              >  2:3502505/1‑150 (MQ=255)
                                                                                        gtgtTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTTCGAATAAGTGGGGAAATTCTTCTCGGCTGAc                                                             >  2:2965543/1‑150 (MQ=255)
                                                                                            tCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAg                                                         >  2:3706622/1‑150 (MQ=255)
                                                                                               gAAATCCAGGCTATTGATTCATCGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGtca                                                      <  1:2675599/150‑1 (MQ=255)
                                                                                                aaaTCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                     <  2:1249066/150‑1 (MQ=255)
                                                                                                   tCCAGGCTATTGATTCAACGTCAGCGACAGATCTGTGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGtcatttc                                                  >  2:107171/1‑150 (MQ=255)
                                                                                                     cAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                <  1:1254038/150‑1 (MQ=255)
                                                                                                      aGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGtcatttcatt                                               >  1:2269948/1‑150 (MQ=255)
                                                                                                       ggCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                              >  1:35161/1‑150 (MQ=255)
                                                                                                       ggCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                              >  2:3999310/1‑150 (MQ=255)
                                                                                                         cTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttct                                            <  1:4611049/150‑1 (MQ=255)
                                                                                                          tATTGAATCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGACTTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGATTAAGTGGGGAGATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                           <  1:1410766/150‑1 (MQ=255)
                                                                                                              gATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                       >  1:2854099/1‑150 (MQ=255)
                                                                                                              gATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                       >  1:4815502/1‑150 (MQ=255)
                                                                                                                ttCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt                                     <  1:4705772/150‑1 (MQ=255)
                                                                                                                 tCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAAGTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                    <  2:1997361/150‑1 (MQ=255)
                                                                                                                      gTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                               >  2:2539083/1‑150 (MQ=255)
                                                                                                                      gTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTCCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                               >  2:4638746/1‑150 (MQ=255)
                                                                                                                       tCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCg                              >  1:2209018/1‑150 (MQ=255)
                                                                                                                            gACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                         <  1:3733744/150‑1 (MQ=255)
                                                                                                                            gACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                         <  2:2298382/150‑1 (MQ=255)
                                                                                                                            gACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                         >  1:4041848/1‑150 (MQ=255)
                                                                                                                            gACAGATCTGCGCTGTAAAAGAACGTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                         <  2:2829352/150‑1 (MQ=255)
                                                                                                                             aCAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                        <  1:4618553/150‑1 (MQ=255)
                                                                                                                              cAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                       >  1:551333/1‑150 (MQ=255)
                                                                                                                               aGATCTGCGCTGTAAATGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                      <  1:4170568/150‑1 (MQ=255)
                                                                                                                               aGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                      <  1:4636637/150‑1 (MQ=255)
                                                                                                                               aGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                      <  1:1330209/150‑1 (MQ=255)
                                                                                                                                gATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtct                     <  2:2631156/150‑1 (MQ=255)
                                                                                                                                gATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtct                     <  2:241380/150‑1 (MQ=255)
                                                                                                                                         tGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt            >  2:1103639/1‑150 (MQ=255)
                                                                                                                                             aaaGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt        <  1:2102239/150‑1 (MQ=255)
                                                                                                                                              aaGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt       <  2:3835198/150‑1 (MQ=255)
                                                                                                                                              aaGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt       <  1:7312/150‑1 (MQ=255)
                                                                                                                                              aaGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt       <  1:2427979/150‑1 (MQ=255)
                                                                                                                                               aGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTg      >  2:71428/1‑150 (MQ=255)
                                                                                                                                                gAATTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa     >  2:1781295/1‑150 (MQ=255)
                                                                                                                                                gAATTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa     >  2:4238290/1‑150 (MQ=255)
                                                                                                                                                gAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa     <  1:6029/150‑1 (MQ=255)
                                                                                                                                                  aCTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt   <  1:344282/150‑1 (MQ=255)
                                                                                                                                                  aCTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt   <  1:588703/150‑1 (MQ=255)
                                                                                                                                                   cTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATc  <  1:2378270/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGTCATTGACAGCGGTTTCTGTGGATTGACACATTTCATTCAGATAATGAATTAATGCTACTGCCGGAACAACCAGCAAACGGGCATTGTGTTCTGAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATC  >  CP009273/2059441‑2059737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑