Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 166,356 C→A 31.3% S31* (TCG→TAG)  fhuC → iron‑hydroxamate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273166,3560CA31.3% 559.2 / 257.2 295S31* (TCG→TAG) fhuCiron‑hydroxamate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (114/88);  new base A (50/43);  total (164/131)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

TTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGAGCAGC  >  CP009273/166208‑166504
                                                                                                                                                    |                                                                                                                                                    
tttCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCg                                                                                                                                                     >  1:960092/1‑150 (MQ=255)
  tCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGtt                                                                                                                                                   >  1:2591634/1‑150 (MQ=255)
  tCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCCTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGtt                                                                                                                                                   <  2:1191667/150‑1 (MQ=255)
     aaTTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAAc                                                                                                                                                >  2:557321/1‑150 (MQ=255)
      aTTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAAcc                                                                                                                                               >  2:3434197/1‑150 (MQ=255)
        ttCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCtt                                                                                                                                             >  2:2156973/1‑150 (MQ=255)
         tctcTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCttt                                                                                                                                            >  2:3848682/1‑150 (MQ=255)
          ctctTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTc                                                                                                                                           >  1:2828442/1‑150 (MQ=255)
             ttttGGGGCACGGATTTCCGTGCCCATTTCAAAATTTGGCTGTTATGCAGTAATACAAGAATAATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCAGCTGTAGTTAACCTTTCCTg                                                                                                                                        <  1:3192054/150‑1 (MQ=255)
                tGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCg                                                                                                                                     >  1:1975210/1‑150 (MQ=255)
                tGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCg                                                                                                                                     >  2:3423565/1‑150 (MQ=255)
                tGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCg                                                                                                                                     <  2:633272/150‑1 (MQ=255)
                  gggCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCggg                                                                                                                                   <  1:3435669/150‑1 (MQ=255)
                       cGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGt                                                                                                                              <  2:1117083/150‑1 (MQ=255)
                       cGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGt                                                                                                                              <  2:4439740/150‑1 (MQ=255)
                         gATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGa                                                                                                                            >  1:1719882/1‑150 (MQ=255)
                              ccGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGt                                                                                                                       <  1:4349092/150‑1 (MQ=255)
                                gTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCt                                                                                                                     <  1:3988013/150‑1 (MQ=255)
                                   cccATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGAt                                                                                                                  >  2:1957167/1‑150 (MQ=255)
                                   cccATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGAt                                                                                                                  >  1:4451918/1‑150 (MQ=255)
                                   cccATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGAt                                                                                                                  <  1:737446/150‑1 (MQ=255)
                                     cATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTg                                                                                                                >  1:1432137/1‑150 (MQ=255)
                                     cATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTGTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGAGTg                                                                                                                >  2:2419430/1‑150 (MQ=255)
                                       tttCACATGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTACCCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGt                                                                                                              <  2:28067/150‑1 (MQ=255)
                                        ttCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTc                                                                                                             <  1:418515/150‑1 (MQ=255)
                                         tCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTca                                                                                                            <  2:4217282/150‑1 (MQ=255)
                                         tCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTca                                                                                                            <  2:1658570/150‑1 (MQ=255)
                                             aaGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAAc                                                                                                        <  1:1973111/150‑1 (MQ=255)
                                              aGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACg                                                                                                       >  1:1743189/1‑150 (MQ=255)
                                               gTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACgg                                                                                                      <  2:2946859/150‑1 (MQ=255)
                                                 tGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGtt                                                                                                    <  2:3500680/150‑1 (MQ=255)
                                                 tGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGtt                                                                                                    >  2:1542051/1‑150 (MQ=255)
                                                 tGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGtt                                                                                                    >  1:2978073/1‑150 (MQ=255)
                                                    cTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTg                                                                                                 <  1:4691281/150‑1 (MQ=255)
                                                     tGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTACTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTgg                                                                                                >  2:511818/1‑150 (MQ=255)
                                                            cAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATcc                                                                                         >  2:1705816/1‑150 (MQ=255)
                                                               gAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCAct                                                                                      <  1:246668/150‑1 (MQ=255)
                                                               gAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCAct                                                                                      <  2:4081147/150‑1 (MQ=255)
                                                               gAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCGGATTGGTCACAACGGTTCTGGTAAATCCAct                                                                                      <  1:4616162/150‑1 (MQ=255)
                                                                  tACACTAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTg                                                                                   <  1:2499721/150‑1 (MQ=255)
                                                                       gAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCaa                                                                              >  2:940809/1‑150 (MQ=255)
                                                                       gAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCaa                                                                              >  1:1549397/1‑150 (MQ=255)
                                                                          tCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAAt                                                                           <  2:802635/150‑1 (MQ=255)
                                                                                 gATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGc                                                                    >  1:3624972/1‑150 (MQ=255)
                                                                                   tACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCg                                                                  >  1:2801551/1‑150 (MQ=255)
                                                                                     ccACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGtc                                                                >  2:3401298/1‑150 (MQ=255)
                                                                                     ccACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGtc                                                                >  1:1287377/1‑150 (MQ=255)
                                                                                       aCTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGtcat                                                              >  1:4270542/1‑150 (MQ=255)
                                                                                         ttttGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGtcatca                                                            >  2:4112842/1‑150 (MQ=255)
                                                                                         ttttGCACTGCGTAATATCTCCTGTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGtcatca                                                            <  2:3757831/150‑1 (MQ=255)
                                                                                            tGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTACTGCCGGGAAAGTGACCGGTCTGATTGGTCCCAACGGTTCTGGTAAATCCACTCTGCTCAAAAAGCTTGGCCGTCATAAgcc                                                         >  1:3772843/1‑150 (MQ=255)
                                                                                             gCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAgccg                                                        >  2:138190/1‑150 (MQ=255)
                                                                                                 tGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGt                                                    >  2:1746254/1‑150 (MQ=255)
                                                                                                 tGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTGCTGGTAAAACCACTCTGCTCAAAATGCTTGGCCGTCCTCCGCCGCCGt                                                    >  2:4568750/1‑150 (MQ=255)
                                                                                                  gCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTc                                                   <  2:1432137/150‑1 (MQ=255)
                                                                                                   cGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCg                                                  <  1:1060359/150‑1 (MQ=255)
                                                                                                    gTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTACAGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTATGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCgg                                                 >  1:110815/1‑150 (MQ=255)
                                                                                                      aaTATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCAACCGCTGTCGTTAACCTTTCATGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATTCTTGTCCGTCAACAGCCGCCGTCGGaa                                               >  1:637708/1‑150 (MQ=255)
                                                                                                       atatCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTTGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCTCCGTCGGAAg                                              >  2:361400/1‑150 (MQ=255)
                                                                                                       atatCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAg                                              <  1:1878494/150‑1 (MQ=255)
                                                                                                         atCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAggg                                            >  1:1017001/1‑150 (MQ=255)
                                                                                                          tctcCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAgggg                                           <  1:2247856/150‑1 (MQ=255)
                                                                                                            tcCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGgag                                         <  1:2385029/150‑1 (MQ=255)
                                                                                                             ccTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGgaga                                        >  1:3471781/1‑150 (MQ=255)
                                                                                                              cTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGAt                                       <  1:511818/150‑1 (MQ=255)
                                                                                                              cTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGAt                                       >  1:813314/1‑150 (MQ=255)
                                                                                                              cTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGAt                                       <  1:2205890/150‑1 (MQ=255)
                                                                                                              cTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCGTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGAt                                       >  1:3323801/1‑150 (MQ=255)
                                                                                                              cTTTCGTGTGCCCGGGCGCACGCGTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGAGTGGTCCCAACGGTTCTGGTAAATCCACTCTGCTCACAATGCTTGGCCGTCATCAGCCGCCGTCGGCAGGGgagct                                       >  2:2608389/1‑148 (MQ=255)
                                                                                                                   gtgtGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCATTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACCGGTCTGGGAAATCCACTCTGCTCAAAATTCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGAttcttc                                  >  1:3374719/1‑150 (MQ=255)
                                                                                                                    tgtgCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGTTTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGAttcttat                                 >  1:2176674/1‑148 (MQ=255)
                                                                                                                    tgtgCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATtcttct                                 >  2:1545238/1‑150 (MQ=255)
                                                                                                                     gtgCCCGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATGGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTcttctt                                <  2:188083/150‑1 (MQ=255)
                                                                                                                         ccGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATg                            <  1:2561194/150‑1 (MQ=255)
                                                                                                                         ccGGGCGCACGCTTTTGCATCCGCTGTAGTTAACCTGTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATg                            <  1:1822697/150‑1 (MQ=255)
                                                                                                                          cGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGc                           >  2:1497746/1‑150 (MQ=255)
                                                                                                                           gggCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGTTTGGTCACAACGGGTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGGGCTTCGTCTTGTTGcc                          >  1:2930564/1‑150 (MQ=255)
                                                                                                                           gggCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGcc                          >  2:3252377/1‑150 (MQ=255)
                                                                                                                           gggCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGcc                          <  2:70517/150‑1 (MQ=255)
                                                                                                                           gggCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTATTGATGcc                          >  2:822306/1‑150 (MQ=255)
                                                                                                                            ggCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGGTTGGTCACAAAGGTTCTGGTAAATACACACTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGCTTCTTCTTGATGccc                         >  1:4090808/1‑150 (MQ=255)
                                                                                                                            ggCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGccc                         >  1:1158486/1‑150 (MQ=255)
                                                                                                                               gcACGCTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAAc                      >  1:4811654/1‑150 (MQ=255)
                                                                                                                                cACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAGAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAAcc                     <  2:3771289/150‑1 (MQ=255)
                                                                                                                                cACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAAcc                     <  2:2248218/150‑1 (MQ=255)
                                                                                                                                cACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAAcc                     <  2:2980247/150‑1 (MQ=255)
                                                                                                                                cACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGGTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGGCGGAAGGGGAGATTCTTCTTGATGCCCAAcc                     >  1:4704813/1‑150 (MQ=255)
                                                                                                                                cACGCTTTTGCATCCGCTGTCGTTAACCATTCCTGCCGGGCAAGTGGCCGGGCTGCTTGGTCACAACGGTTCTGGTAAAAACACTCAGCTCAAAATGCTTGGCCGTCAACAGCCGCCCGCGGCAGGGGAGATTCTTATTGATGCCCCAcc                     >  1:2033490/1‑150 (MQ=255)
                                                                                                                                  cGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCCGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGc                   >  2:2217970/1‑150 (MQ=255)
                                                                                                                                   gCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCt                  >  2:2626252/1‑150 (MQ=255)
                                                                                                                                    cTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTg                 >  2:4700749/1‑150 (MQ=255)
                                                                                                                                    cTTTTGCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTg                 <  2:910367/150‑1 (MQ=255)
                                                                                                                                         gCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTTACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAg            >  1:2708459/1‑150 (MQ=255)
                                                                                                                                         gCATCCGCTGTAGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAg            >  2:3050135/1‑150 (MQ=255)
                                                                                                                                           aTCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCt          <  2:1025006/150‑1 (MQ=255)
                                                                                                                                           aTCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCt          <  2:2036118/150‑1 (MQ=255)
                                                                                                                                              cGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGa       <  2:1975210/150‑1 (MQ=255)
                                                                                                                                                cTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGagc     >  2:2460457/1‑150 (MQ=255)
                                                                                                                                                 tGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGagca    >  1:4313561/1‑150 (MQ=255)
                                                                                                                                                   tCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGagcagc  <  1:4830385/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACCGGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCATCAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGAGCAGC  >  CP009273/166208‑166504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑