Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,216,296 C→A 64.0% I211I (ATC→ATA)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,216,2960CA64.0% 271.4 / inf 319I211I (ATC→ATA) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base C (61/54);  new base A (107/97);  total (168/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 324 total aligned reads displayed.

GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGC  >  CP009273/2216149‑2216445
                                                                                                                                                   |                                                                                                                                                     
 cTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATaaaa                                                                                                                                                    <  2:68298/150‑1 (MQ=255)
   ggTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGa                                                                                                                                                  <  2:90605/150‑1 (MQ=255)
    gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAt                                                                                                                                                 <  1:2226932/150‑1 (MQ=255)
     tGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATg                                                                                                                                                <  2:302468/150‑1 (MQ=255)
             ggCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAtgtg                                                                                                                                        <  2:976814/150‑1 (MQ=255)
                ccGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGt                                                                                                                                     >  2:454479/1‑150 (MQ=255)
                 cGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTc                                                                                                                                    <  2:4040074/150‑1 (MQ=255)
                     gTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGa                                                                                                                                >  1:1228654/1‑150 (MQ=255)
                      tataCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGATGATGTGCGTCACGAt                                                                                                                               >  2:3921841/1‑150 (MQ=255)
                        taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATgg                                                                                                                             >  2:3182963/1‑150 (MQ=255)
                         aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGgt                                                                                                                            >  1:2649591/1‑150 (MQ=255)
                         aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgt                                                                                                                            <  1:3181510/150‑1 (MQ=255)
                           cGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGgtcg                                                                                                                          >  1:448261/1‑150 (MQ=255)
                            gAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgt                                                                                                                         >  1:2859900/1‑150 (MQ=255)
                             aaaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                        >  2:2865685/1‑150 (MQ=255)
                               aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGCCGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCAc                                                                                                                      <  1:2821098/150‑1 (MQ=255)
                               aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCAc                                                                                                                      <  1:4417418/150‑1 (MQ=255)
                                 gTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGc                                                                                                                    >  2:4410371/1‑150 (MQ=255)
                                   cGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGccc                                                                                                                  >  1:861681/1‑150 (MQ=255)
                                       aTTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGa                                                                                                              >  2:2041444/1‑150 (MQ=255)
                                        ttCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAt                                                                                                             >  2:2651576/1‑150 (MQ=255)
                                        ttCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCATCACGATGGTCGTCACGCCCACGAt                                                                                                             <  1:1827248/150‑1 (MQ=255)
                                        ttCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAt                                                                                                             <  2:4569039/150‑1 (MQ=255)
                                        ttCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAt                                                                                                             <  2:2375624/150‑1 (MQ=255)
                                        ttCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGCCACGCCCACGAt                                                                                                             >  1:3681869/1‑150 (MQ=255)
                                          cgcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTa                                                                                                           >  1:4349873/1‑150 (MQ=255)
                                           gcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTAt                                                                                                          <  2:359929/150‑1 (MQ=255)
                                            cgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATg                                                                                                         <  1:2579463/150‑1 (MQ=255)
                                                tatataTGAAGACGGCAACCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTa                                                                                                     <  2:3021070/150‑1 (MQ=255)
                                                  taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATg                                                                                                   >  2:2773223/1‑150 (MQ=255)
                                                  taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATg                                                                                                   >  2:2346932/1‑150 (MQ=255)
                                                   aaaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGt                                                                                                  <  1:1183055/150‑1 (MQ=255)
                                                    aaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTc                                                                                                 <  2:1564427/150‑1 (MQ=255)
                                                    aaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTc                                                                                                 <  1:3839554/150‑1 (MQ=255)
                                                     aTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTcc                                                                                                <  2:564697/150‑1 (MQ=255)
                                                     aTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTcc                                                                                                >  1:1544354/1‑150 (MQ=255)
                                                     aTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTcc                                                                                                >  1:2327402/1‑150 (MQ=255)
                                                      tGAAGACGGCAAACTGACGCTGGTGAACCATCTGTGGATTGATCTGGGCGTATCGCCGGAGCTGATCCTTAGCAAGCTGGATGTTGATCGCATCATAGATGACGATGTGCTTCACGATGGTAGTCACGCCCACGATTATGACTATGtact                                                                                               <  2:1985961/150‑4 (MQ=255)
                                                      tGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCa                                                                                               <  2:870947/150‑1 (MQ=255)
                                                      tGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCa                                                                                               <  2:1475879/150‑1 (MQ=255)
                                                      tGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCa                                                                                               >  1:1723549/1‑150 (MQ=255)
                                                      tGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCa                                                                                               >  2:430194/1‑150 (MQ=255)
                                                       gAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAc                                                                                              >  1:4299559/1‑150 (MQ=255)
                                                        aaGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAcc                                                                                             >  2:109795/1‑150 (MQ=255)
                                                         aGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcg                                                                                            >  1:3235575/1‑150 (MQ=255)
                                                          gACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcgc                                                                                           >  2:2223240/1‑150 (MQ=255)
                                                           aCGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcgcg                                                                                          >  1:3476954/1‑150 (MQ=255)
                                                             ggCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGtt                                                                                        <  2:3534178/150‑1 (MQ=255)
                                                             ggCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGtt                                                                                        <  2:1666940/150‑1 (MQ=255)
                                                             ggCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGtt                                                                                        >  1:298759/1‑150 (MQ=255)
                                                             ggCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGtt                                                                                        >  2:2535247/1‑150 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                       <  1:3594386/150‑1 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                       <  2:61396/150‑1 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                       >  2:2173557/1‑150 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                       <  1:3492126/150‑1 (MQ=255)
                                                                  aCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGa                                                                                   >  1:2395494/1‑150 (MQ=255)
                                                                   cTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGat                                                                                  >  1:2816477/1‑150 (MQ=255)
                                                                   cTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGat                                                                                  >  1:2816072/1‑150 (MQ=255)
                                                                    tGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGata                                                                                 <  1:4549180/150‑1 (MQ=255)
                                                                       cGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTg                                                                              >  1:1043318/1‑150 (MQ=255)
                                                                             tGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCg                                                                        <  1:4208980/150‑1 (MQ=255)
                                                                                aCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGaca                                                                     <  2:3851587/150‑1 (MQ=255)
                                                                                aCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGaca                                                                     <  1:1452887/150‑1 (MQ=255)
                                                                                 ccATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGacac                                                                    <  2:4349873/150‑1 (MQ=255)
                                                                                 ccATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGacac                                                                    <  2:491341/150‑1 (MQ=255)
                                                                                 ccATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGacac                                                                    >  1:3804201/1‑150 (MQ=255)
                                                                                 ccATCTGGGGACTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGacac                                                                    >  2:2626122/1‑150 (MQ=255)
                                                                                     cTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGccc                                                                >  1:893397/1‑150 (MQ=255)
                                                                                            ttGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGtt                                                         <  2:1741348/150‑1 (MQ=255)
                                                                                                tCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATaa                                                     <  2:1916387/150‑1 (MQ=255)
                                                                                                 cTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAAc                                                    <  1:1797950/150‑1 (MQ=255)
                                                                                                          aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCt                                           <  2:3729873/150‑1 (MQ=255)
                                                                                                           cGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTg                                          <  1:773756/150‑1 (MQ=255)
                                                                                                           cGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTg                                          >  2:2300778/1‑150 (MQ=255)
                                                                                                            gCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                         >  1:3323345/1‑150 (MQ=255)
                                                                                                            gCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                         >  2:4127104/1‑150 (MQ=255)
                                                                                                             ccGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGAt                                        >  1:4000247/1‑150 (MQ=255)
                                                                                                             ccGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGAt                                        >  1:477600/1‑150 (MQ=255)
                                                                                                              cGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATc                                       <  1:3373858/150‑1 (MQ=255)
                                                                                                               ggAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGAAGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTAGTGATATTGAAGCCGACACTCCCGCACGTAATAACTCCGATCCTGATCg                                      >  1:628548/1‑150 (MQ=255)
                                                                                                               ggAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCg                                      <  2:764214/150‑1 (MQ=255)
                                                                                                                   cAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTa                                  >  2:2147317/1‑150 (MQ=255)
                                                                                                                    aGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                                 >  2:3970580/1‑150 (MQ=255)
                                                                                                                    aGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                                 <  1:4718012/150‑1 (MQ=255)
                                                                                                                       tCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                              <  2:934228/150‑1 (MQ=255)
                                                                                                                       tCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                              <  2:1416757/150‑1 (MQ=255)
                                                                                                                        ccTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGa                             <  1:1349015/150‑1 (MQ=255)
                                                                                                                         cTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGTTGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGaa                            <  1:2097164/150‑1 (MQ=255)
                                                                                                                         cTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGaa                            >  2:2813505/1‑150 (MQ=255)
                                                                                                                            aGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtct                         <  2:194447/150‑1 (MQ=255)
                                                                                                                            aGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtct                         >  2:3415226/1‑150 (MQ=255)
                                                                                                                             gCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTAATTTGAAtctt                        >  2:115831/1‑150 (MQ=255)
                                                                                                                               aaGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtcttct                      <  2:3387219/150‑1 (MQ=255)
                                                                                                                                   tGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACTATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGtt                  <  1:1532737/150‑1 (MQ=255)
                                                                                                                                   tGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGtt                  <  2:4784619/150‑1 (MQ=255)
                                                                                                                                   tGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGtt                  >  2:3140314/1‑150 (MQ=255)
                                                                                                                                            atCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGa         >  1:1017309/1‑150 (MQ=255)
                                                                                                                                              cGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAg       <  2:3218252/150‑1 (MQ=255)
                                                                                                                                                cATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCt     <  1:4816371/150‑1 (MQ=255)
                                                                                                                                                   cAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGc  <  2:3562939/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGC  >  CP009273/2216149‑2216445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑