Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,092 A→T 23.1% S236R (AGT→AGA)  ccmC ← heme exporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,0920AT23.1% 597.8 / 193.6 266S236R (AGT→AGA) ccmCheme exporter subunit
Reads supporting (aligned to +/- strand):  ref base A (108/95);  new base T (37/25);  total (146/120)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

ATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGA  >  CP009273/2288944‑2289241
                                                                                                                                                    |                                                                                                                                                     
     cGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCAcc                                                                                                                                                 >  1:2915583/1‑150 (MQ=255)
      gAGTGCATGACCGAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCAccc                                                                                                                                                <  1:1670581/150‑1 (MQ=255)
       aGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCa                                                                                                                                               >  1:1634477/1‑150 (MQ=255)
       aGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCa                                                                                                                                               >  2:172903/1‑150 (MQ=255)
         tGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacg                                                                                                                                             >  2:2362885/1‑150 (MQ=255)
         tGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacg                                                                                                                                             <  1:4406169/150‑1 (MQ=255)
           cACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCacgga                                                                                                                                           >  2:1530474/1‑150 (MQ=255)
            aCGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggac                                                                                                                                          <  1:804474/150‑1 (MQ=255)
             cGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCacggacg                                                                                                                                         >  1:2682842/1‑150 (MQ=255)
                 cAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGt                                                                                                                                     >  1:2349389/1‑150 (MQ=255)
                   aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGttt                                                                                                                                   <  1:4744064/150‑1 (MQ=255)
                   aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTGTTCAGTATCAGTTCACTCACCCACGGACGGCGtgt                                                                                                                                   >  2:2576924/1‑148 (MQ=255)
                    aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtttt                                                                                                                                  <  2:2458285/150‑1 (MQ=255)
                    aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtttt                                                                                                                                  <  1:1491999/150‑1 (MQ=255)
                     accaccAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGttttt                                                                                                                                 <  2:960439/150‑1 (MQ=255)
                     accaccAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGttttt                                                                                                                                 <  2:1130338/150‑1 (MQ=255)
                      ccaccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTc                                                                                                                                <  1:1393416/150‑1 (MQ=255)
                       caccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTcc                                                                                                                               <  1:2889742/150‑1 (MQ=255)
                        accaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCa                                                                                                                              <  2:2129648/150‑1 (MQ=255)
                        accaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCa                                                                                                                              <  1:1123178/150‑1 (MQ=255)
                         ccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATACATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCAt                                                                                                                             >  1:4118203/1‑150 (MQ=255)
                            gCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAg                                                                                                                          >  2:2034762/1‑150 (MQ=255)
                                  tAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAAt                                                                                                                    >  2:4722523/1‑150 (MQ=255)
                                  tAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAAt                                                                                                                    >  2:2714629/1‑150 (MQ=255)
                                    aCGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCa                                                                                                                  >  2:2422990/1‑150 (MQ=255)
                                     cGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCaa                                                                                                                 >  1:101295/1‑150 (MQ=255)
                                     cGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCaa                                                                                                                 <  2:3358877/150‑1 (MQ=255)
                                       gTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                                                                               <  1:3362839/150‑1 (MQ=255)
                                        tcatcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAtt                                                                                                              >  1:3605657/1‑150 (MQ=255)
                                         catcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTa                                                                                                             <  1:684142/150‑1 (MQ=255)
                                           tcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcg                                                                                                           <  1:3493918/150‑1 (MQ=255)
                                           tcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcg                                                                                                           <  1:3164424/150‑1 (MQ=255)
                                             accaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                                                                         >  2:3200093/1‑150 (MQ=255)
                                               caccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatc                                                                                                       <  2:1280280/150‑1 (MQ=255)
                                                     cagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                                                                                 >  2:4714307/1‑150 (MQ=255)
                                                      agccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtca                                                                                                <  1:3334437/150‑1 (MQ=255)
                                                            aCAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtca                                                                                          <  2:2816769/150‑1 (MQ=255)
                                                              aaaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                                                                        >  1:1005462/1‑150 (MQ=255)
                                                                 aaaGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGTAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTg                                                                                     <  1:4430202/150‑1 (MQ=255)
                                                                 aaaGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTg                                                                                     >  1:3867934/1‑150 (MQ=255)
                                                                   aGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGc                                                                                   >  1:3730133/1‑150 (MQ=255)
                                                                    ggCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCa                                                                                  <  2:3488970/150‑1 (MQ=255)
                                                                     gCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAg                                                                                 <  1:4865874/150‑1 (MQ=255)
                                                                       gTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                                                                               >  1:525406/1‑150 (MQ=255)
                                                                         aaCCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                                                                             >  1:2977913/1‑150 (MQ=255)
                                                                            cGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAGCAGCGTCAGCGTGGCAGACAGGAg                                                                          >  2:1633714/1‑150 (MQ=255)
                                                                            cGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAg                                                                          >  2:4234716/1‑150 (MQ=255)
                                                                             gCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGc                                                                         <  2:433980/150‑1 (MQ=255)
                                                                              cccATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                                                                        >  1:3029398/1‑150 (MQ=255)
                                                                              cccATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                                                                        >  1:3444243/1‑150 (MQ=255)
                                                                               ccATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                                                                       >  1:2203196/1‑150 (MQ=255)
                                                                               ccATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                                                                       >  1:85083/1‑150 (MQ=255)
                                                                               ccATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                                                                       >  2:3288527/1‑150 (MQ=255)
                                                                                cATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAgg                                                                      <  2:2539861/150‑1 (MQ=255)
                                                                                   tGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAg                                                                   <  2:4481038/150‑1 (MQ=255)
                                                                                   tGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAg                                                                   >  1:1741666/1‑150 (MQ=255)
                                                                                          aaTTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaaa                                                            >  2:2087646/1‑150 (MQ=255)
                                                                                          aaTTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaaa                                                            >  2:4044874/1‑150 (MQ=255)
                                                                                              cattcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGa                                                        >  2:4518464/1‑150 (MQ=255)
                                                                                               attcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAc                                                       >  2:3152455/1‑150 (MQ=255)
                                                                                               attcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAc                                                       <  1:3191286/150‑1 (MQ=255)
                                                                                                ttcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAcc                                                      >  1:1112933/1‑150 (MQ=255)
                                                                                                   cAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagc                                                   >  1:2569127/1‑150 (MQ=255)
                                                                                                     ggAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagcgc                                                 >  1:2288986/1‑150 (MQ=255)
                                                                                                      gAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagcgca                                                <  2:4371466/150‑1 (MQ=255)
                                                                                                       aaGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagcgcag                                               <  2:530863/150‑1 (MQ=255)
                                                                                                        aGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagcgcagc                                              >  1:3126917/1‑150 (MQ=255)
                                                                                                         gCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagcgcagcg                                             >  2:2265969/1‑150 (MQ=255)
                                                                                                         gCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACcagcgcagcg                                             >  1:4773733/1‑150 (MQ=255)
                                                                                                           aaaTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAgcggc                                           >  1:4591546/1‑150 (MQ=255)
                                                                                                            aaTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAgcggcg                                          >  2:1089986/1‑150 (MQ=255)
                                                                                                            aaTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAgcggcg                                          >  2:1056664/1‑150 (MQ=255)
                                                                                                            aaTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAgcggcg                                          >  2:4648463/1‑150 (MQ=255)
                                                                                                            aaTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAgcggcg                                          >  1:1588703/1‑150 (MQ=255)
                                                                                                             aTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGa                                         <  2:3384882/150‑1 (MQ=255)
                                                                                                             aTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGa                                         >  1:608876/1‑150 (MQ=255)
                                                                                                             aTGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATAAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGa                                         <  1:2323263/150‑1 (MQ=255)
                                                                                                                 aGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGc                                     >  2:897914/1‑150 (MQ=255)
                                                                                                                      tCATTTACGGCCTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAACAGCGCAGCGGCGAACGCATCGc                                >  1:4039513/1‑150 (MQ=255)
                                                                                                                       cATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCTTTTTTCCATCAGAAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACCGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGcc                               <  1:4067403/150‑1 (MQ=255)
                                                                                                                         tttACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCgg                             <  2:4118203/150‑1 (MQ=255)
                                                                                                                            aCGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATc                          <  2:708543/150‑1 (MQ=255)
                                                                                                                              ggCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGa                        >  1:2481189/1‑150 (MQ=255)
                                                                                                                                ccTCTTTTCAGTATCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATa                      <  1:2072483/150‑1 (MQ=255)
                                                                                                                                ccTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCCGCGTCAGCGGGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATa                      <  1:4522072/150‑1 (MQ=255)
                                                                                                                                     tttCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtg                 >  1:3744876/1‑150 (MQ=255)
                                                                                                                                       tCATTATCAGTACACACACCCCCGGACGGCGTTTTTCCATCACCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCATACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgct               <  1:172903/150‑1 (MQ=255)
                                                                                                                                       tCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgct               <  2:1966722/150‑1 (MQ=255)
                                                                                                                                       tCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgct               >  2:2979725/1‑150 (MQ=255)
                                                                                                                                       tCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgct               <  2:2342530/150‑1 (MQ=255)
                                                                                                                                         aGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgctgc             >  2:3997781/1‑150 (MQ=255)
                                                                                                                                         aGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgctgc             >  1:4817739/1‑150 (MQ=255)
                                                                                                                                            aTCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATc          >  2:2235236/1‑150 (MQ=255)
                                                                                                                                             tCAGTTCTCTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCCGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATcc         <  2:1634639/150‑1 (MQ=255)
                                                                                                                                             tCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATcc         <  1:4737589/150‑1 (MQ=255)
                                                                                                                                              cAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcg        >  2:4751313/1‑150 (MQ=255)
                                                                                                                                                gTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcgcg      >  1:412313/1‑150 (MQ=255)
                                                                                                                                                gTTCACTCACCCACGGACGGCGTTTTTCCATCAACAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcgcg      <  2:2399314/150‑1 (MQ=255)
                                                                                                                                                    actcacCCACGGACGGCGTTTTTCCATCAGCAAAATCAATTTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGa  <  2:3680852/150‑1 (MQ=255)
                                                                                                                                                    actcacCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGGGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGGTGa  >  2:3650984/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGA  >  CP009273/2288944‑2289241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑