Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,383 G→T 19.9% E304* (GAA→TAA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,3830GT19.9% 931.9 / 185.5 336E304* (GAA→TAA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (149/120);  new base T (35/32);  total (184/152)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 344 total aligned reads displayed.

ATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAG  >  CP009273/2619234‑2619532
                                                                                                                                                     |                                                                                                                                                     
aTTTCGCATCGCTTAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATg                                                                                                                                                       <  1:1484414/150‑1 (MQ=255)
aTTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATg                                                                                                                                                       >  2:39011/1‑150 (MQ=255)
aTTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATg                                                                                                                                                       >  2:460793/1‑150 (MQ=255)
  ttCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGaa                                                                                                                                                     >  1:1047503/1‑150 (MQ=255)
     gCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATg                                                                                                                                                  >  2:3201722/1‑150 (MQ=255)
      cATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATgg                                                                                                                                                 >  1:382628/1‑150 (MQ=255)
            tGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGAc                                                                                                                                           <  2:214552/150‑1 (MQ=255)
             gAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACg                                                                                                                                          <  1:4839582/150‑1 (MQ=255)
             gAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACg                                                                                                                                          >  1:1417691/1‑150 (MQ=255)
              aGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGt                                                                                                                                         <  2:521208/150‑1 (MQ=255)
              aGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGt                                                                                                                                         >  2:4545411/1‑150 (MQ=255)
               gTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGtt                                                                                                                                        <  2:2609487/150‑1 (MQ=255)
                  tACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTCCTGTATTAAATGGAAGGACGTTTcc                                                                                                                                     >  2:4292622/1‑150 (MQ=255)
                  tACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTcc                                                                                                                                     >  2:1838953/1‑150 (MQ=255)
                   aCCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCg                                                                                                                                    <  2:2401303/150‑1 (MQ=255)
                     cGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGtc                                                                                                                                  >  1:1251513/1‑150 (MQ=255)
                     cGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGtc                                                                                                                                  >  1:2556757/1‑150 (MQ=255)
                     cGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGtc                                                                                                                                  <  1:4734651/150‑1 (MQ=255)
                           tttCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAgg                                                                                                                            >  2:2566675/1‑150 (MQ=255)
                               cGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGAtgt                                                                                                                        <  1:826400/150‑1 (MQ=255)
                                 aaGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGc                                                                                                                      >  2:1012105/1‑150 (MQ=255)
                                 aaGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGc                                                                                                                      >  2:4127206/1‑150 (MQ=255)
                                   gagCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGt                                                                                                                    <  2:3399941/150‑1 (MQ=255)
                                   gagCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGt                                                                                                                    <  1:3676968/150‑1 (MQ=255)
                                      cGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGt                                                                                                                 >  1:2173375/1‑150 (MQ=255)
                                      cGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGt                                                                                                                 <  2:2738774/150‑1 (MQ=255)
                                       ggAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTc                                                                                                                >  1:2303826/1‑150 (MQ=255)
                                          aaaCAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCa                                                                                                             >  2:1742633/1‑150 (MQ=255)
                                                tCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCca                                                                                                       <  1:1531716/150‑1 (MQ=255)
                                                tCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCca                                                                                                       <  1:2833598/150‑1 (MQ=255)
                                                tCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCca                                                                                                       >  1:2423285/1‑150 (MQ=255)
                                                tCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCca                                                                                                       <  1:1560709/150‑1 (MQ=255)
                                                 cTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGcccg                                                                                                      >  2:4191398/1‑148 (MQ=255)
                                                   tCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCcagca                                                                                                    <  2:1661773/150‑1 (MQ=255)
                                                   tCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCcagca                                                                                                    <  1:1957855/150‑1 (MQ=255)
                                                    cGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCcagcag                                                                                                   >  2:1284048/1‑150 (MQ=255)
                                                    cGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCcagcag                                                                                                   >  1:4423326/1‑150 (MQ=255)
                                                    cGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTGTCCGTCATCAGGATGTGCGTAGTCGCACCGCcagcag                                                                                                   >  2:431939/1‑150 (MQ=255)
                                                      ttCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGcc                                                                                                 >  2:4807746/1‑150 (MQ=255)
                                                        ccGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTc                                                                                               <  2:1389392/150‑1 (MQ=255)
                                                         cGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCg                                                                                              >  1:4146105/1‑150 (MQ=255)
                                                         cGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCg                                                                                              >  2:4338491/1‑150 (MQ=255)
                                                         cGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCg                                                                                              >  1:894340/1‑150 (MQ=255)
                                                         cGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCg                                                                                              >  1:750628/1‑150 (MQ=255)
                                                               tGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTGGTCGCACCGCCAGCAGCCTCGCCAAcc                                                                                        >  1:1695811/1‑150 (MQ=255)
                                                               tGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAAcc                                                                                        <  2:3984281/150‑1 (MQ=255)
                                                               tGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAAcc                                                                                        >  1:1920376/1‑150 (MQ=255)
                                                                 gCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAg                                                                                      >  1:615531/1‑150 (MQ=255)
                                                                  cGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGt                                                                                     <  2:3507259/150‑1 (MQ=255)
                                                                  cGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGt                                                                                     <  2:2154313/150‑1 (MQ=255)
                                                                  cGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGt                                                                                     <  2:1859625/150‑1 (MQ=255)
                                                                  cGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGt                                                                                     >  1:3715572/1‑150 (MQ=255)
                                                                   gATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTa                                                                                    <  1:4191986/150‑1 (MQ=255)
                                                                    aTTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTAt                                                                                   <  1:2931140/150‑1 (MQ=255)
                                                                      tttATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATca                                                                                 >  2:3805922/1‑150 (MQ=255)
                                                                       ttATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATcac                                                                                <  2:1216701/150‑1 (MQ=255)
                                                                         aTGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACAt                                                                              >  1:2711986/1‑150 (MQ=255)
                                                                          tGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATc                                                                             >  2:2466641/1‑150 (MQ=255)
                                                                          tGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATc                                                                             >  2:2307130/1‑150 (MQ=255)
                                                                           gCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCg                                                                            <  1:782620/150‑1 (MQ=255)
                                                                            cGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGa                                                                           >  1:3607857/1‑150 (MQ=255)
                                                                              gtgtgtTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACa                                                                         <  1:4617256/150‑1 (MQ=255)
                                                                                 tgtTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCg                                                                      <  2:4721680/150‑1 (MQ=255)
                                                                                  gtTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGa                                                                     >  1:4245650/1‑150 (MQ=255)
                                                                                   tttGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGaa                                                                    >  2:2935732/1‑150 (MQ=255)
                                                                                    ttGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAAc                                                                   <  2:2814284/150‑1 (MQ=255)
                                                                                     tGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACa                                                                  <  2:1739378/150‑1 (MQ=255)
                                                                                      gATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAg                                                                 >  1:4272416/1‑150 (MQ=255)
                                                                                      gATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAg                                                                 <  1:3994492/150‑1 (MQ=255)
                                                                                       aTGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAgg                                                                <  2:869810/150‑1 (MQ=255)
                                                                                         gCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGcc                                                              <  1:225812/150‑1 (MQ=255)
                                                                                          cTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGccc                                                             >  1:1799063/1‑150 (MQ=255)
                                                                                           tttAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCg                                                            >  1:3696512/1‑150 (MQ=255)
                                                                                             tAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGAc                                                          >  1:4207938/1‑150 (MQ=255)
                                                                                                 cATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGt                                                      >  1:3179207/1‑150 (MQ=255)
                                                                                                 cATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGt                                                      <  2:3558657/150‑1 (MQ=255)
                                                                                                  aTCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTg                                                     >  2:492623/1‑150 (MQ=255)
                                                                                                   tCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGc                                                    <  2:1954463/150‑1 (MQ=255)
                                                                                                     cGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTg                                                  <  1:1174066/150‑1 (MQ=255)
                                                                                                      gTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTgg                                                 >  1:1731958/1‑150 (MQ=255)
                                                                                                        gAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGAt                                               >  1:3073499/1‑150 (MQ=255)
                                                                                                         aaCTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATa                                              >  1:1128163/1‑150 (MQ=255)
                                                                                                           cTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATAcc                                            <  1:2650376/150‑1 (MQ=255)
                                                                                                               gacTTTCTGACGGGGCGTTACGCGAAGTCTTACTGTATGTAATTGAAGGACGTTTCCGTAATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGAAGGGTGCTGGATACCACTa                                        <  2:3020307/148‑1 (MQ=255)
                                                                                                                ccTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTAt                                       <  1:3845174/150‑1 (MQ=255)
                                                                                                                 cTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATg                                      <  1:4608189/150‑1 (MQ=255)
                                                                                                                  tttCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGc                                     >  1:2470465/1‑150 (MQ=255)
                                                                                                                   ttCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCa                                    >  2:4022819/1‑150 (MQ=255)
                                                                                                                       gACGGGGCGTTACGCGAAGGCGTACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATg                                >  1:3304125/1‑150 (MQ=255)
                                                                                                                       gACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATg                                <  2:3326151/150‑1 (MQ=255)
                                                                                                                       gACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATg                                <  2:791100/150‑1 (MQ=255)
                                                                                                                               gTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACa                        <  2:225106/150‑1 (MQ=255)
                                                                                                                                         ggCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGaa              <  2:1062841/150‑1 (MQ=255)
                                                                                                                                         ggCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGaa              <  1:659810/150‑1 (MQ=255)
                                                                                                                                           cGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACa            >  1:2790936/1‑150 (MQ=255)
                                                                                                                                            gTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAg           <  1:1406346/150‑1 (MQ=255)
                                                                                                                                            gTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAg           >  2:2087632/1‑150 (MQ=255)
                                                                                                                                             tACTGTATTAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGc          <  1:2105140/150‑1 (MQ=255)
                                                                                                                                               cTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCaa        >  1:932760/1‑150 (MQ=255)
                                                                                                                                                  tATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCg     >  2:1424471/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGGAAAACAGTCTTCGTTCCGGGACTGGCGATTTTATGCGGTGTGTTTGATGCTTTAGCCATCCGTGAACTGCGCCTTTCTGACGGGGCGTTACGCGAAGGCGTACTGTATGAAATGGAAGGACGTTTCCGTCATCAGGATGTGCGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAG  >  CP009273/2619234‑2619532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑