BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,633,091	C→A	5.7%	V282L (GTG→TTG)	hisS ←	histidyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,633,091	0	C	A	5.7%	1132.4 / 34.4	300	V282L (GTG→TTG)	hisS	histidyl tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (157/126); new base A (9/8); total (166/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 317 total aligned reads displayed.

TATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATT  >  CP009273/2632942‑2633240

tatatCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCacacac                                                                                                                                                       <  1:4401621/150‑1 (MQ=255)

 atatCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACACGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACg                                                                                                                                                      <  2:2497817/150‑1 (MQ=255)

     cGACAACCGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACACGACGTTCGCGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGCCCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGc                                                                                                                                                  <  1:4109782/150‑1 (MQ=255)

     cGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGc                                                                                                                                                  >  2:516066/1‑150 (MQ=255)

           caGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTggg                                                                                                                                            >  1:4328639/1‑150 (MQ=255)

            aGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGa                                                                                                                                           >  1:4843363/1‑150 (MQ=255)

             ggATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAg                                                                                                                                          >  2:1279429/1‑150 (MQ=255)

              gATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACAAGGTGCCCTGGGAGc                                                                                                                                         <  2:158108/150‑1 (MQ=255)

               aTCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGcc                                                                                                                                        >  1:4009458/1‑150 (MQ=255)

                     tttAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACt                                                                                                                                  >  2:3356656/1‑150 (MQ=255)

                     tttAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACt                                                                                                                                  >  2:4336505/1‑150 (MQ=255)

                      ttAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACTACCGCCTGCACACACGGAGCCATGGGAGCCGAGACTg                                                                                                                                 >  1:1358551/1‑150 (MQ=255)

                      ttAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACAAGGTGCCCTGGGAGCCGAGACTg                                                                                                                                 <  2:1671791/150‑1 (MQ=255)

                        aaaTTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGtt                                                                                                                               <  1:545988/150‑1 (MQ=255)

                          aTTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAg                                                                                                                             >  1:1975404/1‑150 (MQ=255)

                          aTTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAg                                                                                                                             <  2:3193043/150‑1 (MQ=255)

                          aTTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAg                                                                                                                             <  1:2821760/150‑1 (MQ=255)

                           ttCCGGATTAACGGCCTGTACTACCAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGt                                                                                                                            <  2:4478347/150‑1 (MQ=255)

                               ggATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCAcc                                                                                                                        >  1:2922548/1‑150 (MQ=255)

                                 aTTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCa                                                                                                                      >  2:1922397/1‑150 (MQ=255)

                                 aTTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCa                                                                                                                      >  2:793691/1‑150 (MQ=255)

                                 aTTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCa                                                                                                                      >  1:3619966/1‑150 (MQ=255)

                                 aTTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCa                                                                                                                      >  1:3939324/1‑150 (MQ=255)

                                  ttAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCAc                                                                                                                     >  1:2116748/1‑150 (MQ=255)

                                   tAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACt                                                                                                                    >  2:2390579/1‑150 (MQ=255)

                                     aCGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCg                                                                                                                  <  1:4530350/150‑1 (MQ=255)

                                       ggCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGaa                                                                                                                >  2:4209338/1‑150 (MQ=255)

                                       ggCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGaa                                                                                                                >  2:2275275/1‑150 (MQ=255)

                                              aCTAACAATACAAGACGTTCGAGGCCAATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggta                                                                                                         <  1:107501/150‑1 (MQ=255)

                                               cTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggtac                                                                                                        >  1:3776106/1‑150 (MQ=255)

                                               cTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggtac                                                                                                        >  1:332366/1‑150 (MQ=255)

                                               cTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACAAGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggtac                                                                                                        >  2:866852/1‑150 (MQ=255)

                                                tAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggtacg                                                                                                       >  2:59886/1‑150 (MQ=255)

                                                 aaCAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggtacgg                                                                                                      >  1:1715090/1‑150 (MQ=255)

                                                 aaCAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCCGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAacggtacgg                                                                                                      >  1:3000341/1‑150 (MQ=255)

                                                   ccaTACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGtt                                                                                                    <  2:4798153/148‑1 (MQ=255)

                                                      tACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTgta                                                                                                 <  2:1767552/150‑1 (MQ=255)

                                                       aCAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCAGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTgtag                                                                                                >  1:4152254/1‑150 (MQ=255)

                                                        cAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTCCGGTTgtagt                                                                                               >  2:3697174/1‑150 (MQ=255)

                                                         aaGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTgtagta                                                                                              >  1:4870294/1‑150 (MQ=255)

                                                           gACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAAt                                                                                            >  1:4796404/1‑150 (MQ=255)

                                                           gACGTTCGAGGCCCATACCAACACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAAt                                                                                            <  1:2865473/150‑1 (MQ=255)

                                                            aCGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATc                                                                                           <  2:1470631/150‑1 (MQ=255)

                                                            aCGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATc                                                                                           <  1:697139/150‑1 (MQ=255)

                                                              gTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCa                                                                                         >  1:141164/1‑150 (MQ=255)

                                                                   aGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGGGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCa                                                                                    <  2:4786572/150‑1 (MQ=255)

                                                                   aGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTCCGGTTGTAGTAATCCAGACCa                                                                                    >  2:2874269/1‑150 (MQ=255)

                                                                   aGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCa                                                                                    >  2:2731014/1‑150 (MQ=255)

                                                                        cATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCAc                                                                               <  2:4423482/150‑1 (MQ=255)

                                                                         aTAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCAcc                                                                              >  1:4750302/1‑150 (MQ=255)

                                                                           aGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAg                                                                            >  2:480567/1‑150 (MQ=255)

                                                                            gCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGa                                                                           <  1:1144569/150‑1 (MQ=255)

                                                                                 accgaccgCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTg                                                                      <  2:3326031/150‑1 (MQ=255)

                                                                                 aCCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTg                                                                      >  2:673071/1‑150 (MQ=255)

                                                                                     aCAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGttt                                                                  >  2:1239884/1‑150 (MQ=255)

                                                                                      cAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTa                                                                 <  2:697111/150‑1 (MQ=255)

                                                                                      cAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTa                                                                 <  2:1634191/150‑1 (MQ=255)

                                                                                      cAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTa                                                                 <  2:3546204/150‑1 (MQ=255)

                                                                                       aGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTAc                                                                <  2:234456/150‑1 (MQ=255)

                                                                                          cGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGgt                                                             <  2:3668355/150‑1 (MQ=255)

                                                                                          cGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGgt                                                             >  1:2390196/1‑150 (MQ=255)

                                                                                               ttGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAg                                                        <  1:225016/150‑1 (MQ=255)

                                                                                               ttGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACAAGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAg                                                        >  2:4654606/1‑150 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCg                                                      <  1:1174995/150‑1 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCg                                                      <  2:3631840/150‑1 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCg                                                      <  2:1358581/150‑1 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCg                                                      >  2:4021030/1‑150 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCg                                                      <  1:4242203/150‑1 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCg                                                      >  1:4581605/1‑150 (MQ=255)

                                                                                                 gCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACAAGGTGCCCTGGGAGCCGAGACTGATAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAATCg                                                      >  1:2145296/1‑150 (MQ=255)

                                                                                                  cACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGa                                                     <  2:4484864/150‑1 (MQ=255)

                                                                                                    cGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATc                                                   >  1:2505620/1‑150 (MQ=255)

                                                                                                     gACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATcc                                                  >  2:2259031/1‑150 (MQ=255)

                                                                                                       ccGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATcccc                                                <  2:3138184/150‑1 (MQ=255)

                                                                                                       ccGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATcccc                                                >  1:2558961/1‑150 (MQ=255)

                                                                                                         gCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCcgc                                              >  2:340766/1‑150 (MQ=255)

                                                                                                          cccAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCcgcg                                             >  1:1541772/1‑150 (MQ=255)

                                                                                                            cAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGct                                           >  1:2737244/1‑150 (MQ=255)

                                                                                                              gttgttCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCTCGctct                                         <  2:2574498/150‑1 (MQ=255)

                                                                                                                 gttCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCca                                      <  2:3459221/150‑1 (MQ=255)

                                                                                                                 gttCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCca                                      >  1:2682406/1‑150 (MQ=255)

                                                                                                                   tCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCCCCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCcagc                                    >  2:1470146/1‑150 (MQ=255)

                                                                                                                    ccACAAGACCGTCATAACGACCGCCTGCACACAAGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCcagca                                   >  2:10376/1‑150 (MQ=255)

                                                                                                                     cacaAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCcagcag                                  >  1:4442492/1‑150 (MQ=255)

                                                                                                                      acaAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGt                                 <  2:3244081/150‑1 (MQ=255)

                                                                                                                         aGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTg                              >  2:4184239/1‑150 (MQ=255)

                                                                                                                           aCCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGca                            <  1:1279429/150‑1 (MQ=255)

                                                                                                                            ccGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGcac                           >  1:3443338/1‑150 (MQ=255)

                                                                                                                             cGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGcaca                          <  1:4034005/150‑1 (MQ=255)

                                                                                                                              gTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAg                         >  1:3788164/1‑150 (MQ=255)

                                                                                                                              gTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAg                         >  1:1323765/1‑150 (MQ=255)

                                                                                                                                cATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGAc                       >  2:3884007/1‑150 (MQ=255)

                                                                                                                                  tAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCg                     >  1:3147187/1‑150 (MQ=255)

                                                                                                                                  tAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCg                     <  1:1877095/150‑1 (MQ=255)

                                                                                                                                        ccGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCaaaa               >  2:215075/1‑150 (MQ=255)

                                                                                                                                          gCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATg             <  1:258025/150‑1 (MQ=255)

                                                                                                                                                acacacGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACg       >  1:4648788/1‑150 (MQ=255)

                                                                                                                                                  acacGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACgag     >  2:2958285/1‑150 (MQ=255)

                                                                                                                                                  acacGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACgag     >  2:1168270/1‑150 (MQ=255)

                                                                                                                                                   cacGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACgaga    >  2:731922/1‑150 (MQ=255)

TATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATACAAGACGTTCGAGGCCCATAGCAAAACCGACAGCCGGTGTTGCACGACCGCCCAGTTGTTCCACAAGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATT  >  CP009273/2632942‑2633240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`