Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,673 A→C 11.4% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,6730AC11.4% 609.4 / 11.7 241V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (149/57);  new base C (2/33);  total (151/90)
Fisher's exact test for biased strand distribution p-value = 2.89e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA  >  CP009273/2711524‑2711822
                                                                                                                                                       |                                                                                                                                                     
tctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGaaa                                                                                                                                                       >  1:4330348/1‑150 (MQ=255)
tctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGaaa                                                                                                                                                       <  1:4395921/150‑1 (MQ=255)
tctGGAAAACTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGaaa                                                                                                                                                       >  2:2168952/1‑150 (MQ=255)
 ctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAAc                                                                                                                                                      >  2:802272/1‑150 (MQ=255)
 ctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAAc                                                                                                                                                      >  1:2179942/1‑150 (MQ=255)
  tGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAAcc                                                                                                                                                     <  2:381510/150‑1 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCga                                                                                                                                                   <  1:291808/150‑2 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                   >  2:746876/1‑150 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                   <  1:1374053/150‑1 (MQ=255)
         gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGcc                                                                                                                                              <  2:2831297/150‑1 (MQ=255)
          tGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCa                                                                                                                                             <  2:2305080/150‑1 (MQ=255)
            ccTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACg                                                                                                                                           >  2:4739588/1‑150 (MQ=255)
                  aTGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAAt                                                                                                                                     >  2:79979/1‑150 (MQ=255)
                    gCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                                                                                                   >  2:2362611/1‑150 (MQ=255)
                     aaTTTTCGCCTTAGACGCGGTTTTCTTCCGCCCGCGGACGACGAAACAGTTCGGTATAAATAAAAATTTAACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCACACCACCGGGCGCGCGGGAAACCGTGCGCCACGGCGAATCtt                                                                                                                                  <  2:3084631/149‑1 (MQ=255)
                      ttttcCGCCTTAGACGCGGGTTTCTTCCGCAGGCTTACGACCCAACAATCCCGCATCAAAAAAAATTTTCCCCCTGATGCCACCG‑TGATCGGCATTTTCC‑GGCGACAAATCACCCGGCGCGGGGGAACCCGTGCCCCACGGCGAATCTTc                                                                                                                                 <  2:378269/149‑1 (MQ=255)
                      aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                                                                                                                 >  2:4127181/1‑150 (MQ=255)
                       ttttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTcc                                                                                                                                >  2:2628835/1‑150 (MQ=255)
                        tttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTccc                                                                                                                               >  2:1160411/1‑150 (MQ=255)
                         ttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCa                                                                                                                              >  2:1451340/1‑150 (MQ=255)
                          tCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCAc                                                                                                                             >  2:330727/1‑150 (MQ=255)
                          tCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCAc                                                                                                                             >  2:2771590/1‑150 (MQ=255)
                           cGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTCCCACt                                                                                                                            >  2:539772/1‑150 (MQ=255)
                            gCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGACTCTTCCCACtt                                                                                                                           >  2:620054/1‑150 (MQ=255)
                              cGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGc                                                                                                                         >  1:3392343/1‑150 (MQ=255)
                               gTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                                                                                                        >  2:1005864/1‑150 (MQ=255)
                               gTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGAGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                                                                                                        <  2:272371/150‑1 (MQ=255)
                                          tttCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                                                                                                             >  2:2162328/1‑150 (MQ=255)
                                          tttCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                                                                                                             >  1:2478370/1‑150 (MQ=255)
                                          tttCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                                                                                                             >  1:1902237/1‑150 (MQ=255)
                                           ttcttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg                                                                                                            >  1:1618736/1‑150 (MQ=255)
                                             cttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                          >  1:911643/1‑150 (MQ=255)
                                              ttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                         >  2:525872/1‑150 (MQ=255)
                                              tgccGCACGCTGCCCACCAAACACTTCCGGATCAATAAAAATTTTACCACTGAGGCCCCGG‑TGATCGGCCTTTGCC‑GGCGTCTCACCACCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                         <  2:3975663/148‑1 (MQ=255)
                                                ccGCACGCTGCCGACGCAACACTGCCGGATCAATAAAACTTTTCCCCGTGTGGCCCCGG‑TGAGCGGCCTTTGCC‑GGCGTCTCATCCCCGGGGGCGGGGGAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                                                                                                       <  2:1584827/150‑1 (MQ=255)
                                                ccGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                                                                                                       >  2:3442891/1‑150 (MQ=255)
                                                 cGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTg                                                                                                      >  2:4235947/1‑150 (MQ=255)
                                                   cACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt                                                                                                    >  2:3394705/1‑150 (MQ=255)
                                                    aCGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                                                                                                   >  1:4254307/1‑150 (MQ=255)
                                                            gacgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                                                           >  1:26731/1‑150 (MQ=255)
                                                            gacgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                                                           >  2:557680/1‑150 (MQ=255)
                                                              cgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgg                                                                                         >  2:45026/1‑150 (MQ=255)
                                                                 aaCACTTCCGTAAAAATAAAACTTTTACCACTGATCCCACGG‑TGATCGGCCTTTGCC‑GGCGACTCATCCCCCGGCGGGCGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                      <  2:1034100/150‑1 (MQ=255)
                                                                  acacTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg                                                                                     >  2:646785/1‑150 (MQ=255)
                                                                   cacTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg                                                                                    >  2:3544062/1‑150 (MQ=255)
                                                                    acTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcgggg                                                                                   >  2:1759425/1‑150 (MQ=255)
                                                                     cTTCCGTACCAATAAAACTTTTACCACTGAGGCCACCG‑TGATCGGCCTTTGCC‑GGCGTCTCATCCCCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                  <  2:1452778/150‑1 (MQ=255)
                                                                      ttCGGGAACAATAAAACTTTACCCAGTGCTGCCCGGG‑TTCGCGGCGTTTCCG‑GGGGTCTCACCCCCGGGCGCGCGCCAACCCGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggcg                                                                                 <  1:3059827/150‑1 (MQ=255)
                                                                      ttCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggcg                                                                                 >  2:3214588/1‑150 (MQ=255)
                                                                      ttCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggcg                                                                                 >  1:3190793/1‑150 (MQ=255)
                                                                        ccGGAGAAAAAAAATTTTTCCCACTGATGCCACGG‑TGATGGGCCTTTGCC‑GGCGTCTCAACCCCGGGCGCGGGGGAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGAc                                                                               <  2:1731713/150‑1 (MQ=255)
                                                                            aaaaaaaaaaTTTTAACCCCTGATCCCCCGG‑TG‑TCCGCCATTTCCGGGCGTCACACCACCGGGCGGGCGGCACCCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGcc                                                                           <  2:1279134/144‑1 (MQ=255)
                                                                               aaTAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                        >  2:1694003/1‑150 (MQ=255)
                                                                                aTAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                       >  2:781578/1‑150 (MQ=255)
                                                                                aTAAAACTTTCACCCCTGA‑GCCCCCGTTGAGCGGCCTTTGCG‑GGCGTCACACCACCGGGCGCGGGGTAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                       <  2:2917506/150‑1 (MQ=255)
                                                                                    aaCTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAg                                                                   >  1:3578747/1‑150 (MQ=255)
                                                                                     aCTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGGCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGc                                                                  >  1:4494323/1‑150 (MQ=255)
                                                                                      cTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                 >  1:2808385/1‑150 (MQ=255)
                                                                                         ttACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACga                                                              >  1:1179691/1‑150 (MQ=255)
                                                                                            ccACTGCTCCCACCG‑TGACGGGCCTTTCCG‑GGCTACTCAACCCCCGGCGCGGGGCAACCCGCGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAt                                                           <  1:378410/150‑1 (MQ=255)
                                                                                             cACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGACTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTGTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                                                          >  2:4715631/1‑150 (MQ=255)
                                                                                              aCTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAttt                                                         >  2:2832227/1‑150 (MQ=255)
                                                                                               ccgaggCCACCG‑TTATCGCCCTTTGCG‑GGCGACACCCCCCCCGCCGCGGGGCAAACCGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        <  1:2008605/145‑1 (MQ=255)
                                                                                                tGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                       >  2:210480/1‑150 (MQ=255)
                                                                                                tGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                       >  2:1218414/1‑150 (MQ=255)
                                                                                                tGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                       >  2:1656202/1‑150 (MQ=255)
                                                                                                    gcccggg‑TGATCGTCCTTTTCG‑GGCGTCTCCTCACCGGGCGCGGGGGACCCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                                                   <  2:3121953/144‑1 (MQ=255)
                                                                                                     ccACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGa                                                  >  1:3923970/1‑150 (MQ=255)
                                                                                                         cG‑TGAGCGGCTTTGTCG‑GGCGTCTCACCCCCGGGCGGGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc                                              <  2:2079065/150‑1 (MQ=255)
                                                                                                            tGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg                                            >  2:3684992/1‑150 (MQ=255)
                                                                                                             gATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                           >  2:3881969/1‑150 (MQ=255)
                                                                                                               tCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACgcccgccc                                         <  2:3190793/150‑1 (MQ=255)
                                                                                                               gcGGCCTTTGCG‑GGCGTCTCATCACCCGGCGCGCGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACgcccgccc                                         <  2:2940610/149‑1 (MQ=255)
                                                                                                                 ggCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                       >  2:1395125/1‑150 (MQ=255)
                                                                                                                   ccTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                     >  1:61112/1‑150 (MQ=255)
                                                                                                                    cTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCCGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtgt                                    >  2:3636945/1‑148 (MQ=255)
                                                                                                                     ttttCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                   >  1:223615/1‑150 (MQ=255)
                                                                                                                       ttCC‑GGCTTCTCATCACCGGGCGCGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                 <  2:329230/150‑1 (MQ=255)
                                                                                                                        tgcgggggTCTCATCACCCGGCGCGCGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                 <  2:2654096/143‑1 (MQ=255)
                                                                                                                         cc‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCgg                               >  2:2122178/1‑150 (MQ=255)
                                                                                                                              ggTCTCATCCCCGGGGGGGGGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                           <  2:3053244/149‑1 (MQ=255)
                                                                                                                              cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                           >  1:2732382/1‑150 (MQ=255)
                                                                                                                                   catccCCCGGGGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                      <  2:2407896/150‑1 (MQ=255)
                                                                                                                                   caccccccGGGGCGCGGCAAACGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                      <  1:1869286/145‑1 (MQ=255)
                                                                                                                                    atcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                     >  1:4655935/1‑150 (MQ=255)
                                                                                                                                    atcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                     >  1:2594940/1‑150 (MQ=255)
                                                                                                                                    atcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                     >  1:3557773/1‑150 (MQ=255)
                                                                                                                                     ccaCCGGGCGGGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                    <  2:1529446/149‑1 (MQ=255)
                                                                                                                                          cGGCGCGCGGGAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGt               <  1:1461623/150‑1 (MQ=255)
                                                                                                                                            gcgcgcGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTCCGCGGGTTGTGGGTAc             >  1:15540/1‑150 (MQ=255)
                                                                                                                                               ggcgGTAAACCGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGtt          <  1:1040621/149‑1 (MQ=255)
                                                                                                                                                 cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa        >  2:94392/1‑150 (MQ=255)
                                                                                                                                                 cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa        <  2:1891354/150‑1 (MQ=255)
                                                                                                                                                 cgGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa        <  2:3558680/150‑1 (MQ=255)
                                                                                                                                                  ggtaaaCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt       <  1:3016643/147‑1 (MQ=255)
                                                                                                                                                  gggAACCCGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt       <  1:635656/150‑1 (MQ=255)
                                                                                                                                                  gggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt       >  1:489677/1‑150 (MQ=255)
                                                                                                                                                   tgAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc      <  1:2362611/149‑1 (MQ=255)
                                                                                                                                                    gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa     >  1:2556617/1‑150 (MQ=255)
                                                                                                                                                     aaaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg    >  2:616142/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCG‑TGATCGGCCTTTTCC‑GGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA  >  CP009273/2711524‑2711822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑