Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,744,009 T→A 27.6% D20V (GAT→GTT)  grpE ← heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,744,0090TA27.6% 412.3 / 198.3 217D20V (GAT→GTT) grpEheat shock protein
Reads supporting (aligned to +/- strand):  ref base T (66/91);  new base A (21/39);  total (87/130)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACAT  >  CP009273/2743860‑2744155
                                                                                                                                                     |                                                                                                                                                  
ccATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAATCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAt                                                                                                                                                    <  1:3471280/150‑1 (MQ=255)
     tCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGGAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATg                                                                                                                                               <  2:2991083/150‑1 (MQ=255)
     tCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATg                                                                                                                                               >  1:1364633/1‑150 (MQ=255)
     tCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATg                                                                                                                                               >  1:1364755/1‑150 (MQ=255)
     tCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATg                                                                                                                                               >  1:2316622/1‑150 (MQ=255)
        gCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATa                                                                                                                                            >  1:4074353/1‑150 (MQ=255)
         cTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATaa                                                                                                                                           >  1:2188181/1‑150 (MQ=255)
         cTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATaa                                                                                                                                           >  1:1146121/1‑150 (MQ=255)
           tttACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAAtt                                                                                                                                         <  2:4601378/150‑1 (MQ=255)
            ttACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAAttt                                                                                                                                        <  1:3469613/150‑1 (MQ=255)
                 cGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTcc                                                                                                                                   <  2:19349/150‑1 (MQ=255)
                 cGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTcc                                                                                                                                   >  1:3092577/1‑150 (MQ=255)
                  gCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCg                                                                                                                                  <  2:4237218/150‑1 (MQ=255)
                     aaaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCgggg                                                                                                                               >  2:3079518/1‑150 (MQ=255)
                      aaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGc                                                                                                                              <  2:1404368/150‑1 (MQ=255)
                        tGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCtt                                                                                                                            <  1:810306/150‑1 (MQ=255)
                        tGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCtt                                                                                                                            <  2:2454828/150‑1 (MQ=255)
                        tGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCtt                                                                                                                            <  2:2833124/150‑1 (MQ=255)
                          ccGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGc                                                                                                                          <  2:4608462/150‑1 (MQ=255)
                           cGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGcc                                                                                                                         >  1:3999587/1‑150 (MQ=255)
                           cGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGcc                                                                                                                         >  1:3875249/1‑150 (MQ=255)
                              cACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCt                                                                                                                      <  1:1933547/150‑1 (MQ=255)
                              cACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCt                                                                                                                      <  1:1907274/150‑1 (MQ=255)
                               aCGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTc                                                                                                                     <  1:2349300/150‑1 (MQ=255)
                                  ttCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAgg                                                                                                                  >  1:3441745/1‑150 (MQ=255)
                                   tCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGc                                                                                                                 >  2:2380319/1‑150 (MQ=255)
                                     aCGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGt                                                                                                               <  1:1194389/150‑1 (MQ=255)
                                     aCGGGTCTGGGCTGCAGCCAGCTGAGCTTCGAGATTCGCAACTTGGTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGt                                                                                                               <  1:2727668/150‑1 (MQ=255)
                                      cGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGtt                                                                                                              <  1:1869932/150‑1 (MQ=255)
                                        ggTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGtttt                                                                                                            <  2:3050348/150‑1 (MQ=255)
                                        ggTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGtttt                                                                                                            <  2:618430/150‑1 (MQ=255)
                                          tCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCt                                                                                                          <  2:3980729/150‑1 (MQ=255)
                                           cTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTg                                                                                                         <  1:4244339/150‑1 (MQ=255)
                                           cTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTg                                                                                                         <  1:571741/150‑1 (MQ=255)
                                           cTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTg                                                                                                         <  1:3095911/150‑1 (MQ=255)
                                           cTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTg                                                                                                         <  2:724080/150‑1 (MQ=255)
                                               gCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCt                                                                                                     <  2:1700058/150‑1 (MQ=255)
                                                cTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCtt                                                                                                    <  2:1625754/150‑1 (MQ=255)
                                                 ttCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCttt                                                                                                   <  2:365148/150‑1 (MQ=255)
                                                 ttCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCttt                                                                                                   <  1:2572737/150‑1 (MQ=255)
                                                   catccagcTGAGATTCGAGATTCGAAACTTTTTCTTCTCGCTGATCCACCTGCTCAGCAGATGCTTCTGGATCAACTGCCTCAATCTATTAGTGCTGATCCATGATTATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTtag                                                                                                 <  2:4449307/147‑2 (MQ=255)
                                                   cagccagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTtac                                                                                                 >  2:705355/1‑150 (MQ=255)
                                                    agccagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTGTGtact                                                                                                >  1:1050854/1‑150 (MQ=255)
                                                    agccagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTtact                                                                                                >  1:3615991/1‑150 (MQ=255)
                                                      ccagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTtactac                                                                                              <  1:2354098/150‑1 (MQ=255)
                                                       cagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTactact                                                                                             >  2:507081/1‑150 (MQ=255)
                                                          cTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTGGGTTGTTTTGTACTCAt                                                                                          >  1:80584/1‑150 (MQ=255)
                                                            gAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGa                                                                                        <  2:4706106/150‑1 (MQ=255)
                                                              gCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAAt                                                                                      <  1:4866943/150‑1 (MQ=255)
                                                                   gagaTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTtctc                                                                                 <  2:4024082/150‑1 (MQ=255)
                                                                   gagaTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTtctc                                                                                 >  2:395224/1‑150 (MQ=255)
                                                                    agaTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTcc                                                                                <  2:3447739/150‑1 (MQ=255)
                                                                     gaTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCcg                                                                               <  1:89736/150‑1 (MQ=255)
                                                                     gaTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCcg                                                                               <  1:3836318/150‑1 (MQ=255)
                                                                      aTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCcgc                                                                              <  1:476714/150‑1 (MQ=255)
                                                                       ttCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCcgcg                                                                             >  1:2415234/1‑150 (MQ=255)
                                                                         cGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGtt                                                                           <  1:2759234/150‑1 (MQ=255)
                                                                         cGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGtt                                                                           <  2:2005049/150‑1 (MQ=255)
                                                                         cGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGtt                                                                           >  2:1763769/1‑150 (MQ=255)
                                                                         cGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGGTTTCTGTTCTTTACTAGTCATGAATGTCTCCGCGtt                                                                           >  2:1763003/1‑150 (MQ=255)
                                                                           cAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGtttt                                                                         <  2:3473418/150‑1 (MQ=255)
                                                                                ttttCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCg                                                                    <  1:2164565/150‑1 (MQ=255)
                                                                                 tttCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGc                                                                   <  2:1537351/150‑1 (MQ=255)
                                                                                    cATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCAtt                                                                <  1:416596/150‑1 (MQ=255)
                                                                                    cATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGGTCTTTACTACGCATGAATTTCTCCGCGTTTTTTTCGCAtt                                                                >  1:766655/1‑150 (MQ=255)
                                                                                       cgcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAt                                                             <  1:1435840/150‑1 (MQ=255)
                                                                                       cgcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAt                                                             >  2:1044384/1‑150 (MQ=255)
                                                                                       cgcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAt                                                             >  2:3495180/1‑150 (MQ=255)
                                                                                        gcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtc                                                            <  1:4868772/150‑1 (MQ=255)
                                                                                         cgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtct                                                           <  2:2041736/150‑1 (MQ=255)
                                                                                         cgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtct                                                           >  2:2132781/1‑150 (MQ=255)
                                                                                         cgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtct                                                           <  1:1985198/150‑1 (MQ=255)
                                                                                         cgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTGTGTTCTTTTCTACAACTTACTTTATCCGCGTTTTTTTATCAGTCAtat                                                           >  1:3251867/1‑148 (MQ=255)
                                                                                            ggATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGc                                                        >  2:2502031/1‑150 (MQ=255)
                                                                                              aTCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGACTTTCTCCGCGTTTTTTTCGCATTCATCTCGCTa                                                      >  2:3230299/1‑150 (MQ=255)
                                                                                                  aCCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACtt                                                  <  1:312283/150‑1 (MQ=255)
                                                                                                    cTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTGTGTTGTTTTATACTCATTAATTTGTCCGCGTTTTTTTATGATTCATCTAGCTAAATTCg                                                >  1:3801824/1‑150 (MQ=255)
                                                                                                    cTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTATCCGCGTTTTTTTAGCATTCATCTCGCGAACTTCg                                                >  2:3693857/1‑150 (MQ=255)
                                                                                                       cTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCtt                                             <  1:4352199/150‑1 (MQ=255)
                                                                                                          agcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                          <  2:310863/150‑1 (MQ=255)
                                                                                                          agcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                          <  2:309704/150‑1 (MQ=255)
                                                                                                          agcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                          <  2:2195962/150‑1 (MQ=255)
                                                                                                             agAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATg                                       <  1:3594781/150‑1 (MQ=255)
                                                                                                             agAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTAGCATTCATCTAGCTAACTTCGCGTATTATg                                       >  1:3343572/1‑150 (MQ=255)
                                                                                                             agAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATg                                       >  2:4229596/1‑150 (MQ=255)
                                                                                                               aaGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTGTGTTCTTTTATACTCATGTGTTTCTCCGCGTTTTTTTAGCATTCATCTCGCTAACTTCGCGTATTATggg                                     >  1:4281910/1‑150 (MQ=255)
                                                                                                               aaGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTCTTGTGTTTTATACTCATTTATTTGTACGCGTTTTTTTAGCATTAATATCGCTAAAATCGAGtattatttg                                     >  1:4526940/1‑147 (MQ=255)
                                                                                                                      tGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGt                              <  2:1313149/150‑1 (MQ=255)
                                                                                                                       ggCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGtt                             <  1:3814693/150‑1 (MQ=255)
                                                                                                                       ggCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGtt                             <  1:322242/150‑1 (MQ=255)
                                                                                                                        gCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCGTATTATGGGGATCAGttt                            >  2:3428610/1‑150 (MQ=255)
                                                                                                                               tGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCGGTTCTTTACAACTCAAGAATTTCTCCGCGTTTTTTTAGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggggt                     >  2:3796361/1‑148 (MQ=255)
                                                                                                                                gCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGttt                    <  1:3079487/150‑1 (MQ=255)
                                                                                                                                gCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGttt                    <  1:3057395/150‑1 (MQ=255)
                                                                                                                                  cTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCa                  <  2:4074353/150‑1 (MQ=255)
                                                                                                                                      aTCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTGTGTTGTTTTCTACTCCTGAATTTCTCCGCGTTTTTTTGGCATTCATATCGCTAACTTCGCTTATTATGGGGGTCAGTTTAAGGGTTTCAAggg              >  1:2535224/1‑150 (MQ=255)
                                                                                                                                          ctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGc          >  2:2811678/1‑150 (MQ=255)
                                                                                                                                             cGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACt       <  2:4219868/150‑1 (MQ=255)
                                                                                                                                                gCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACATCGCTTATTATGGGGATAAGTTTCAGGGTTTAAAGGGAAGCAcaaac    >  2:2453560/1‑146 (MQ=255)
                                                                                                                                                  tGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACAt  >  2:1877776/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACAT  >  CP009273/2743860‑2744155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑