Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,934,843 G→T 69.4% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,934,8430GT69.4% 364.1 / 287.6 280H50N (CAT→AAT) ygdDUPF0382 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (50/36);  new base T (113/81);  total (163/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

CGCCCACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCAT  >  CP009273/2934694‑2934990
                                                                                                                                                     |                                                                                                                                                   
     acaAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACg                                                                                                                                                >  1:1204712/1‑150 (MQ=255)
       aaaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGcc                                                                                                                                              >  2:1661716/1‑150 (MQ=255)
        aaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCt                                                                                                                                             <  2:1084159/150‑1 (MQ=255)
        aaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCt                                                                                                                                             <  2:679190/150‑1 (MQ=255)
         aCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTg                                                                                                                                            <  1:2666611/150‑1 (MQ=255)
         aCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTg                                                                                                                                            <  2:4222453/150‑1 (MQ=255)
          cGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTgg                                                                                                                                           >  1:4333794/1‑150 (MQ=255)
              gttGGGACAGCGCCAGGAAATACCGGCTGCCGCTGAACAACCCGGTGCCTACCGCGAGGAAAACGCTACTCCAGTAAAACCCGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTAtt                                                                                                                                       <  1:3292091/148‑1 (MQ=255)
              gATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTAtt                                                                                                                                       >  2:229140/1‑150 (MQ=255)
              gATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGAGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCAAGCGTATGAAACGCCTGGTAtt                                                                                                                                       >  1:3619550/1‑150 (MQ=255)
                  ggACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAg                                                                                                                                   >  2:4290947/1‑150 (MQ=255)
                           cAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTg                                                                                                                          <  1:2108565/150‑1 (MQ=255)
                            aGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTgg                                                                                                                         >  2:1142703/1‑150 (MQ=255)
                             ggCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGa                                                                                                                        >  2:2372600/1‑150 (MQ=255)
                               cAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCCCCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATc                                                                                                                      >  2:3872373/1‑150 (MQ=255)
                                 aTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCa                                                                                                                    >  1:1999318/1‑150 (MQ=255)
                                  tAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAg                                                                                                                   >  1:730228/1‑150 (MQ=255)
                                     aGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGccc                                                                                                                >  1:1754733/1‑150 (MQ=255)
                                         tGCCGCTGAACAACACCGTGCCTACCGCGAGGAAAACGCTACTCCAGTAAAACCAGCTACTGATGCGCCGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                            <  1:1484523/150‑1 (MQ=255)
                                          gCCGCTGAACAACACCGTGCCTAACGCGAGTAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtctc                                                                                                           <  1:507056/150‑1 (MQ=255)
                                           ccGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCa                                                                                                          >  1:3177503/1‑150 (MQ=255)
                                             gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                        <  2:1501318/150‑1 (MQ=255)
                                             gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                        >  1:3641164/1‑150 (MQ=255)
                                             gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                        <  1:2212434/150‑1 (MQ=255)
                                              cTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACg                                                                                                       <  2:2406414/150‑1 (MQ=255)
                                              cTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACg                                                                                                       <  2:2819829/150‑1 (MQ=255)
                                               tGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACgg                                                                                                      >  1:677925/1‑150 (MQ=255)
                                                   caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                  >  2:1467778/1‑150 (MQ=255)
                                                   caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                  <  2:1314752/150‑1 (MQ=255)
                                                    aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                 >  1:2253268/1‑150 (MQ=255)
                                                       acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                              <  2:1590306/150‑1 (MQ=255)
                                                       acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                              >  2:2920875/1‑150 (MQ=255)
                                                          gTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGtt                                                                                           <  2:4585486/150‑1 (MQ=255)
                                                             ccTAACGATAGTAAATCGCTAGTCCAGTAATACCTGATACTGTTGCGACGCTGCATTGACACCGCATGAACTAAGCTCGCCAGCGTATTAATGGCCTGGTATTCGAGGACGGTCTGGATCCAGACCATCTCAACGGCCCCCATGGTTTTa                                                                                        <  2:4384312/150‑1 (MQ=255)
                                                                aaCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACtt                                                                                     <  1:1543395/150‑1 (MQ=255)
                                                                aaCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACtt                                                                                     >  2:1749829/1‑150 (MQ=255)
                                                                 aCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTa                                                                                    <  2:268458/150‑1 (MQ=255)
                                                                   gcgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAac                                                                                  >  2:1393635/1‑150 (MQ=255)
                                                                     gAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAacac                                                                                >  2:2807284/1‑150 (MQ=255)
                                                                      aGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAAcaca                                                                               >  2:2477457/1‑150 (MQ=255)
                                                                      aGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAAcaca                                                                               >  1:3871701/1‑150 (MQ=255)
                                                                       ggAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACAt                                                                              >  1:3399075/1‑150 (MQ=255)
                                                                        gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                             <  2:2250834/150‑1 (MQ=255)
                                                                        gAAAACGATACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                             <  1:2883413/150‑1 (MQ=255)
                                                                          aaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcg                                                                           <  1:2418740/150‑1 (MQ=255)
                                                                          aaaCGCTACGCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcg                                                                           <  1:564593/150‑1 (MQ=255)
                                                                           aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                          >  2:1769531/1‑150 (MQ=255)
                                                                           aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                          >  2:1840727/1‑150 (MQ=255)
                                                                           aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                          >  2:3196377/1‑150 (MQ=255)
                                                                              gCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGcc                                                                       <  1:3600598/150‑1 (MQ=255)
                                                                               cTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCa                                                                      <  1:925679/150‑1 (MQ=255)
                                                                                   tCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAg                                                                  >  2:468528/1‑150 (MQ=255)
                                                                                    ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                 <  2:873701/150‑1 (MQ=255)
                                                                                        tAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGccc                                                             >  1:2142125/1‑150 (MQ=255)
                                                                                         aaaaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCa                                                            >  1:2298543/1‑150 (MQ=255)
                                                                                               aGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCca                                                      >  1:2616438/1‑150 (MQ=255)
                                                                                               aGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCca                                                      >  1:2509603/1‑150 (MQ=255)
                                                                                                 aTACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCTTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                    <  1:1667676/150‑1 (MQ=255)
                                                                                                 aTACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                    <  1:317097/150‑1 (MQ=255)
                                                                                                 aTACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                    >  2:3660170/1‑150 (MQ=255)
                                                                                                    cTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaaa                                                 >  1:281387/1‑150 (MQ=255)
                                                                                                     tGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaaaa                                                >  1:98736/1‑150 (MQ=255)
                                                                                                      gATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAAt                                               >  2:4184379/1‑150 (MQ=255)
                                                                                                        tGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGa                                             >  2:475807/1‑150 (MQ=255)
                                                                                                           gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                          <  1:1749829/150‑1 (MQ=255)
                                                                                                           gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                          >  1:4720360/1‑150 (MQ=255)
                                                                                                           gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                          >  1:3310300/1‑150 (MQ=255)
                                                                                                              gCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGc                                       >  2:3583948/1‑150 (MQ=255)
                                                                                                               cTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCt                                      >  1:940556/1‑150 (MQ=255)
                                                                                                                tGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTa                                     >  1:1288654/1‑150 (MQ=255)
                                                                                                                tGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTa                                     >  2:1262075/1‑150 (MQ=255)
                                                                                                                tGCATTGCCACCGCAAGACCTAAGATCGCCAGCTTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTAAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTa                                     <  1:3027909/150‑1 (MQ=255)
                                                                                                                 gCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTaa                                    >  2:4099026/1‑150 (MQ=255)
                                                                                                                   aTTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATg                                  >  2:3155767/1‑150 (MQ=255)
                                                                                                                    ttGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATgg                                 >  2:3437142/1‑150 (MQ=255)
                                                                                                                     tGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggc                                >  2:635487/1‑150 (MQ=255)
                                                                                                                      gCCACCGCCATACCTAATATCGCCAGCGTTTTAAACGCCTGTTATTCGAGGCCGGTCTGGATCCAGCCAATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcg                               <  1:2094264/150‑1 (MQ=255)
                                                                                                                      gCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcg                               >  2:3103190/1‑150 (MQ=255)
                                                                                                                      gCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCATGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcg                               <  2:3778255/150‑1 (MQ=255)
                                                                                                                      gCCACCGCAAGACCTAAGATCGCCAGCGTATTAAAGGCCTGGTATTCGTGGCCGGTCTGGATCCTGCCCATCTCAACGGCCCACATGGTTTTACTTAACCCATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAAGggcg                               <  1:1875545/150‑1 (MQ=255)
                                                                                                                       ccACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcgg                              >  1:2067771/1‑150 (MQ=255)
                                                                                                                        cACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcggc                             >  1:4764552/1‑150 (MQ=255)
                                                                                                                            gCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaa                         >  2:3124338/1‑150 (MQ=255)
                                                                                                                            gCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaa                         <  2:644265/150‑1 (MQ=255)
                                                                                                                             ccAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaaa                        >  2:724762/1‑150 (MQ=255)
                                                                                                                              cAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAAt                       >  1:460043/1‑150 (MQ=255)
                                                                                                                              cAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAAt                       <  2:4652294/150‑1 (MQ=255)
                                                                                                                               agcccTAAGATCGCCAGCGTATTACACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCACCGGCCCCCAGGGTTTTACTTAACACATGCGCGCCAAACGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATc                      <  2:957987/147‑1 (MQ=255)
                                                                                                                               aGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATc                      >  2:3285133/1‑150 (MQ=255)
                                                                                                                                    tAATATCGCCAGCGTATTAAACGCCTGTTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCAt                 <  1:4673120/150‑1 (MQ=255)
                                                                                                                                    tAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCAt                 >  2:4036852/1‑150 (MQ=255)
                                                                                                                                     aaGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa                <  2:2621106/150‑1 (MQ=255)
                                                                                                                                      aGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaa               >  1:4244371/1‑150 (MQ=255)
                                                                                                                                      aGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTGTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaa               >  2:2950312/1‑150 (MQ=255)
                                                                                                                                        aTCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaaaa             <  1:3237369/150‑1 (MQ=255)
                                                                                                                                          cGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACg           <  1:2169593/150‑1 (MQ=255)
                                                                                                                                              aGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTg       <  2:4523326/150‑1 (MQ=255)
                                                                                                                                               gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGTATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTgg      <  2:77608/150‑1 (MQ=255)
                                                                                                                                                 gTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTc    >  2:204423/1‑150 (MQ=255)
                                                                                                                                                  tATGAAACGCCTGTTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAACGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCa   <  2:1445367/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCCCACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCAT  >  CP009273/2934694‑2934990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑