Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,940,834 A→T 8.2% intergenic (+12/‑22) metZ → / → metW tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,940,8340AT 8.2% 437.9 / 4.0 159intergenic (+12/‑22)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (50/89);  new base T (4/12);  total (55/104)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCT  >  CP009273/2940684‑2940983
                                                                                                                                                      |                                                                                                                                                       
gTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                                                                                         >  2:2316815/1‑150 (MQ=255)
  gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTtgat                                                                                                                                                        >  1:2745658/1‑150 (MQ=255)
      ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAg                                                                                                                                                   <  1:3856113/150‑1 (MQ=255)
      ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAg                                                                                                                                                   <  1:1395823/150‑1 (MQ=255)
      ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAg                                                                                                                                                   <  1:2023295/150‑1 (MQ=255)
      ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAg                                                                                                                                                   <  1:420135/150‑1 (MQ=255)
        ggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑Ta                                                                                                                                                <  2:568671/150‑1 (MQ=255)
         gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGGAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGTTG‑AAG‑Taa                                                                                                                                               >  2:3351313/1‑150 (MQ=255)
         gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑Taa                                                                                                                                               <  2:409095/150‑1 (MQ=255)
         gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑Taa                                                                                                                                               <  2:408339/150‑1 (MQ=255)
            aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGc                                                                                                                                            <  1:2420185/150‑1 (MQ=255)
             aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCa                                                                                                                                           >  2:383451/1‑150 (MQ=255)
             aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCa                                                                                                                                           <  1:4391418/150‑1 (MQ=255)
              aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAg                                                                                                                                          <  2:3317633/150‑1 (MQ=255)
              aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTCACGCAAG‑AAAAGCAg                                                                                                                                          >  2:3290428/1‑150 (MQ=18)
               aaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTTGATG‑AAG‑TAAAGCAGt                                                                                                                                         >  2:170437/1‑150 (MQ=255)
                aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTa                                                                                                                                        <  2:3926071/150‑1 (MQ=255)
                aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCCAAGCTCGTCTGATCCAATCCCCCACCCGCAACCAATGAAATTGGTATT‑AGG‑AAAAGCAGTa                                                                                                                                        >  1:1052879/1‑150 (MQ=18)
                aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACAACACTGTCGCCGCTTCAATTCAGACCCACGCAACCAATTCAAATATCAGG‑AGG‑TAGTGCAGTa                                                                                                                                        >  2:2719661/1‑150 (MQ=21)
                 aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTTAAATTTTGATG‑AAG‑TAAAGCAGTAc                                                                                                                                       >  1:3096278/1‑150 (MQ=255)
                 aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGG‑AAG‑TAAAGCAGTAc                                                                                                                                       >  2:1476377/1‑150 (MQ=255)
                  aGGTTGCATGAAAACGCGAGCGGAGTATAGTGCACATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCCCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACg                                                                                                                                      <  1:3673607/150‑1 (MQ=255)
                    gTTTCATGAAAACGCGAGCGGAGTATAGTGCTCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGATCATAACCAGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTTGATG‑AAG‑TAAAGCAGTACGGt                                                                                                                                    <  2:256436/150‑1 (MQ=18)
                     ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTg                                                                                                                                   <  1:4784514/150‑1 (MQ=255)
                                 gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGa                                                                                                                       <  2:1750884/150‑1 (MQ=255)
                                    aGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGatca                                                                                                                    >  2:1609069/1‑150 (MQ=255)
                                       ggaggaTAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTACAAAC‑AAG‑CACGTCAGTACGGGGACCGGGGGTGGAGCAAcc                                                                                                                 >  1:2794703/1‑150 (MQ=14)
                                       ggAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                 >  1:4548867/1‑150 (MQ=255)
                                       ggAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                 <  1:667345/150‑1 (MQ=255)
                                          gTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                              >  1:2719180/1‑150 (MQ=255)
                                           tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                                                                                                             <  1:1494815/150‑1 (MQ=255)
                                            ataGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                            <  2:3282341/150‑1 (MQ=255)
                                            ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAGAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                            <  2:2089705/150‑1 (MQ=255)
                                             taGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg                                                                                                           <  1:2144150/150‑1 (MQ=255)
                                                   gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                                                                     <  2:473895/150‑1 (MQ=255)
                                                    cATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAGTTTCAGG‑AAG‑AATGGCATTACGGTGTAGAGGGGTGGAGCAGACAGGTAGCtctacg                                                                                                    >  2:4005284/1‑146 (MQ=14)
                                                     aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTTTTG‑TAGTTAATGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                                                                   >  1:2140821/1‑150 (MQ=11)
                                                     aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCACATAACCCGAAGGTCGTAGGTGCAAATCCCGACCCCGCAACCACTAAAAAGTTGATG‑AAG‑GAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTCGCTCGTCgg                                                                                                   >  1:3940570/1‑150 (MQ=14)
                                                         aCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTACCGATCCGGCCCCCGAAACCAATACAATCAACCTG‑GCG‑TAAAGCAGTACGGTCACGCGGGGGGGAGCAGCCTGGTAGCTCGGCGGGcgc                                                                                               >  1:3173979/1‑148 (MQ=11)
                                                          cGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                              <  2:4326203/150‑1 (MQ=255)
                                                          cGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGAAGCCTGGTAGCTCGTCGGGCTCa                                                                                              >  1:253061/1‑150 (MQ=255)
                                                            gACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                                                                            <  2:558769/150‑1 (MQ=40)
                                                                 ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTGGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                                       >  1:2639296/1‑150 (MQ=255)
                                                                    gTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAg                                                                                    <  1:2633829/150‑1 (MQ=40)
                                                                    gTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAg                                                                                    <  1:532998/150‑1 (MQ=40)
                                                                       gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAggcc                                                                                 <  1:3221172/150‑3 (MQ=255)
                                                                         gcagcCTGGTATCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                               <  1:4209993/150‑1 (MQ=255)
                                                                         gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                               <  1:1950489/150‑1 (MQ=40)
                                                                          cagcCTTGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgtc                                                                              <  1:1899190/150‑1 (MQ=255)
                                                                               tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTc                                                                         <  2:680382/150‑1 (MQ=40)
                                                                               tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTc                                                                         <  1:3216156/150‑1 (MQ=40)
                                                                                ggTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCa                                                                        <  2:3060197/150‑1 (MQ=40)
                                                                                ggTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCa                                                                        <  1:2263380/150‑1 (MQ=40)
                                                                                    gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                                    <  1:3450259/150‑1 (MQ=40)
                                                                                       cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TACAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                                 >  2:888032/1‑150 (MQ=255)
                                                                                          cgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGccc                                                              <  2:1971845/150‑1 (MQ=40)
                                                                                             gCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCg                                                           <  2:3749671/150‑1 (MQ=40)
                                                                                                  tAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGTTG‑AAG‑TCAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                                      <  2:1033455/150‑1 (MQ=255)
                                                                                                   aaCCCGAAGGTCGTCGGTTCATATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                     <  2:767075/150‑1 (MQ=255)
                                                                                                   aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                     >  1:3135090/1‑150 (MQ=40)
                                                                                                   aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                     <  1:2599502/150‑1 (MQ=40)
                                                                                                     cccGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACGAATTAAAATTTGATG‑TAG‑TTATGCACTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                   <  2:2024443/150‑1 (MQ=14)
                                                                                                      ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCAGGCCCCCGCAACCAAtt                                                  >  1:2785425/1‑149 (MQ=255)
                                                                                                       cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCa                                                 <  1:3293175/150‑1 (MQ=255)
                                                                                                       cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATCa                                                 >  2:241246/1‑150 (MQ=255)
                                                                                                       cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑AAATGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACACGAAGGGCGACGGTTCAAATCCGGCCCCCGCACCCCATCa                                                 >  2:3441405/1‑150 (MQ=14)
                                                                                                        gAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCCGCCCCCGCAACCaaaaaa                                                >  2:4547035/1‑146 (MQ=255)
                                                                                                          atgtcgtaTGTTCTAATCCGGACCCCTCAAACAATTATATTTTGTTG‑TAG‑TAAAGCAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                              <  1:4770465/148‑1 (MQ=14)
                                                                                                           ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAtt                                             <  2:3330708/150‑1 (MQ=255)
                                                                                                            gtcgtcGGTTCAAATCCGTCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt                                            <  1:4515512/150‑1 (MQ=255)
                                                                                                                tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgatg                                        <  1:383451/150‑1 (MQ=255)
                                                                                                                  ggTTCTAATCCTTCAGCCGCAAACAATTATAATTTGTTG‑AAG‑TTAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGaa                                      <  1:196226/150‑1 (MQ=17)
                                                                                                                  ggTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGGGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGaa                                      <  1:1173764/150‑1 (MQ=255)
                                                                                                                  ggTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGaa                                      >  1:513563/1‑150 (MQ=255)
                                                                                                                      cAAATCCGGCCCCCGCAACCAATTAAATTTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaa                                  <  2:1220629/150‑1 (MQ=255)
                                                                                                                      cAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaa                                  <  2:1220460/150‑1 (MQ=255)
                                                                                                                      cAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAACCAATCAAATAGGAGGAAGTac                                  >  2:3776740/1‑149 (MQ=255)
                                                                                                                       ataTCCTGACCCCTCAAACAATTATAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaaa                                 <  1:1004472/148‑1 (MQ=17)
                                                                                                                         aTCCTGCACCCGCAACCAATTAAATTGTGTTG‑TAG‑TAAAGGAGTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAg                               <  2:4602248/150‑1 (MQ=17)
                                                                                                                         aTCCGTCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAg                               <  2:2152717/150‑1 (MQ=255)
                                                                                                                          tCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGc                              <  2:513563/150‑1 (MQ=255)
                                                                                                                             ttaccccGCAACCAATTATATTTTGTTG‑AAG‑TATTGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGt                           <  1:144326/147‑1 (MQ=18)
                                                                                                                             ggCCCCCGCAACCAATTATACTTTGATG‑AAG‑TAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGt                           <  2:2871487/150‑1 (MQ=255)
                                                                                                                             ggCCCCCGCAACCAAATCTATATTGTTG‑AAG‑TCATGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGt                           <  2:2871537/150‑1 (MQ=17)
                                                                                                                              gCCCCCTCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                          <  2:3091980/150‑1 (MQ=255)
                                                                                                                              gCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                          >  1:141943/1‑150 (MQ=255)
                                                                                                                               cccccGCAACCAATTATAATTTGATG‑AAG‑TAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTAc                         <  2:4745828/150‑1 (MQ=255)
                                                                                                                                 cccGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTCATAGCAGTAAgg                       >  2:4457676/1‑150 (MQ=255)
                                                                                                                                 cccGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACgg                       <  1:2317273/150‑1 (MQ=255)
                                                                                                                                  ccGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAATAGCAGTACGGt                      >  1:3920707/1‑150 (MQ=255)
                                                                                                                                     cAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAc                   >  1:458712/1‑150 (MQ=255)
                                                                                                                                      aaCCAATTAAATTTTGATG‑TAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGCCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAcg                  <  2:4870886/150‑1 (MQ=255)
                                                                                                                                      aaCCAATTAAATTTTGATG‑AAG‑TTTTGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGCCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAcg                  <  2:4871958/150‑1 (MQ=18)
                                                                                                                                           aTTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTAAAAGCAGTACGGTGACGCgggg             >  2:1916012/1‑150 (MQ=255)
                                                                                                                                            ttAAAATTTGATG‑AAG‑TACAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGt            <  1:3621427/150‑1 (MQ=255)
                                                                                                                                                aaTTTTATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAAGAAGTAAAAGCAGTAAGGTGACGCGGGGTGGag        >  2:1065677/1‑150 (MQ=255)
                                                                                                                                                aaTTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGag        >  1:1785696/1‑150 (MQ=255)
                                                                                                                                                   ttGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagcag     >  2:1596545/1‑150 (MQ=255)
                                                                                                                                                     gatg‑aAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATCAAATTTCATCAAGTAAAAGCAGTACGGTGACGAGGGCTGGAGCAGcc   >  1:1686692/1‑150 (MQ=18)
                                                                                                                                                      atg‑aAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTAAAATCTTTAAGTTGTAGCGGGGTGGAGAAGCCt  >  1:3123719/1‑150 (MQ=17)
                                                                                                                                                      |                                                                                                                                                       
GTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAG‑TAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCT  >  CP009273/2940684‑2940983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑