Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,088,517 C→A 11.2% R21S (CGT→AGT)  yggS → UPF0001 family protein, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,088,5170CA11.2% 886.8 / 68.9 258R21S (CGT→AGT) yggSUPF0001 family protein, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (124/105);  new base A (15/14);  total (139/119)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 260 total aligned reads displayed.

CAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACTT  >  CP009273/3088369‑3088662
                                                                                                                                                    |                                                                                                                                                 
     gcTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCAATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCg                                                                                                                                             <  1:1433527/150‑1 (MQ=255)
       tGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGACAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCCGCGGCTGCAACGCGTTGCGGc                                                                                                                                           >  2:1656704/1‑150 (MQ=255)
         cacaGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCg                                                                                                                                         <  2:4858631/150‑1 (MQ=255)
               tgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTcc                                                                                                                                   <  2:2687885/150‑1 (MQ=255)
               tgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATAGTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTcc                                                                                                                                   >  2:377773/1‑150 (MQ=255)
                ttAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGTAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCa                                                                                                                                  <  1:2074016/149‑1 (MQ=255)
                 tAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCag                                                                                                                                 >  2:329514/1‑150 (MQ=255)
                     tCCGAGACGTTATGCTTTACACTAAGGGCCACATTTTCTTCAATATTCATACTAATATCCTCGTAAAATTAACGATATTGCGCATAACCTGGAACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCagaaga                                                                                                                             <  1:3041179/150‑1 (MQ=255)
                        gagaCGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAAt                                                                                                                          <  1:4310358/150‑1 (MQ=255)
                        gagaCGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAAt                                                                                                                          <  2:1538575/150‑1 (MQ=255)
                           aCGTTATGCTTTACACTAAGGGCCACAATTTCTTCAATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTAc                                                                                                                       <  1:2324411/150‑1 (MQ=255)
                            cGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACg                                                                                                                      >  1:840656/1‑150 (MQ=255)
                             gTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgc                                                                                                                     >  2:3847199/1‑150 (MQ=255)
                              ttATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgct                                                                                                                    >  2:3879482/1‑150 (MQ=255)
                              ttATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgct                                                                                                                    >  2:3680624/1‑150 (MQ=255)
                               tATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATTTTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgctg                                                                                                                   <  1:3508998/150‑1 (MQ=255)
                                   cTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTg                                                                                                               <  1:3772881/150‑1 (MQ=255)
                                   cTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTg                                                                                                               <  1:1311724/150‑1 (MQ=255)
                                   cTTAACCCTAAGGGCCACAATTTCTTCCATATTCATAATCAGATCCTCGGAAAATGAACGATATTGCGCATAACCGGGCACTGGTCCGGGACAAAATCCCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTg                                                                                                               <  1:1649076/150‑1 (MQ=255)
                                       acacTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAATAAATTACGCTGCTTGcagt                                                                                                           >  1:4486505/1‑150 (MQ=255)
                                       acacTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagt                                                                                                           >  1:241995/1‑150 (MQ=255)
                                         acTAAGGGCCACAATTTCTTCCATATTCATCCTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagtca                                                                                                         <  1:1519814/150‑1 (MQ=255)
                                            aaGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTTCTTTCAGTCAGTa                                                                                                      >  1:1664550/1‑150 (MQ=255)
                                             aGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaa                                                                                                     >  1:2917113/1‑150 (MQ=255)
                                                   acaATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaa                                                                                               >  2:879473/1‑150 (MQ=255)
                                                    caATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaa                                                                                              >  1:3059180/1‑150 (MQ=255)
                                                    caATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaa                                                                                              >  2:1228979/1‑150 (MQ=255)
                                                     aaTTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaac                                                                                             >  2:1881848/1‑150 (MQ=255)
                                                     aaTTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaac                                                                                             >  2:3893447/1‑150 (MQ=255)
                                                     aaTTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaac                                                                                             <  2:431689/150‑1 (MQ=255)
                                                     aaTTTCGTCAATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaac                                                                                             <  1:2107772/150‑1 (MQ=255)
                                                     aaTTACTTCCAAATTCATACTAAGATCCTCGTAAAATGAACTATATTGCGAATACCCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaaacaaaac                                                                                             <  1:4595638/150‑1 (MQ=255)
                                                      aTTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGACAGTAAAACAAAAcc                                                                                            >  1:4628394/1‑150 (MQ=255)
                                                       tttCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCt                                                                                           >  1:3759432/1‑150 (MQ=255)
                                                         tcttcCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGc                                                                                         <  1:4447040/150‑1 (MQ=255)
                                                           ttcCATATTCATACTAAGATCCTCGTAAAATGAACGATATTGCGAATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGa                                                                                       <  1:108240/150‑1 (MQ=255)
                                                           ttcCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGa                                                                                       >  1:1191064/1‑150 (MQ=255)
                                                             ccATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAgc                                                                                     <  1:2293586/150‑1 (MQ=255)
                                                             ccATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAgc                                                                                     >  1:2360553/1‑150 (MQ=255)
                                                              cATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAgcg                                                                                    <  2:1976269/150‑1 (MQ=255)
                                                               atatTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAgcgc                                                                                   >  1:4547087/1‑150 (MQ=255)
                                                                tatTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGcc                                                                                  >  2:2038337/1‑150 (MQ=255)
                                                                 atTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCa                                                                                 >  2:3019365/1‑150 (MQ=255)
                                                                  ttCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCAt                                                                                <  1:4052872/150‑1 (MQ=255)
                                                                   tCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATc                                                                               <  2:2710181/150‑1 (MQ=255)
                                                                   tCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATc                                                                               >  1:2102893/1‑150 (MQ=255)
                                                                     aTACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGcccacgc                                                                             >  1:3277731/1‑145 (MQ=255)
                                                                       aCTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAg                                                                           >  1:1461042/1‑150 (MQ=255)
                                                                            gATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGcc                                                                      >  2:3645367/1‑150 (MQ=255)
                                                                            gATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGcc                                                                      >  1:732178/1‑150 (MQ=255)
                                                                             aTCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCa                                                                     <  2:4269889/150‑1 (MQ=255)
                                                                                cTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGAAAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTg                                                                  <  1:1026279/150‑1 (MQ=255)
                                                                                 tCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGa                                                                 >  2:1427042/1‑150 (MQ=255)
                                                                                   ggAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATg                                                               >  2:131228/1‑150 (MQ=255)
                                                                                   ggAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATg                                                               <  2:2917021/150‑1 (MQ=255)
                                                                                    gAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGc                                                              <  2:1142035/150‑1 (MQ=255)
                                                                                    gAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGc                                                              >  1:527914/1‑150 (MQ=255)
                                                                                     aaaaTGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGcc                                                             >  2:2983926/1‑150 (MQ=255)
                                                                                     aaaaTGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGcc                                                             >  2:2983075/1‑150 (MQ=255)
                                                                                       aaTGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCgg                                                           <  2:764315/150‑1 (MQ=255)
                                                                                         tGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCAACGCAGAAGCCATAGATGCCGGGc                                                         >  1:4107919/1‑150 (MQ=255)
                                                                                          gAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCa                                                        >  2:2789956/1‑150 (MQ=255)
                                                                                           aaCGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAg                                                       >  2:4809747/1‑150 (MQ=255)
                                                                                               atatTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTc                                                   <  2:2757350/150‑1 (MQ=255)
                                                                                                 atTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCaa                                                 >  2:3148980/1‑150 (MQ=255)
                                                                                                  ttGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAAt                                                >  2:3534076/1‑150 (MQ=255)
                                                                                                  ttGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAAt                                                <  1:2514646/150‑1 (MQ=255)
                                                                                                    gcgcATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAAttt                                              <  2:1786305/150‑1 (MQ=255)
                                                                                                      gcATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTgg                                            >  1:925582/1‑150 (MQ=255)
                                                                                                       cATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGt                                           <  1:3900486/150‑1 (MQ=255)
                                                                                                        aTAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTg                                          <  2:1752389/150‑1 (MQ=255)
                                                                                                           aCCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGaaa                                       <  1:3518416/150‑1 (MQ=255)
                                                                                                           aCCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGaaa                                       <  2:562586/150‑1 (MQ=255)
                                                                                                            ccTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATGTGGTGaaaa                                      >  2:1385256/1‑150 (MQ=255)
                                                                                                              tGGCACAGGTCCGGGACAAAATCTCCGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACt                                    <  1:613813/150‑1 (MQ=255)
                                                                                                              tGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGTTGCCGGGCAGCGTCAATTTGGTGAAAACt                                    >  2:398866/1‑150 (MQ=255)
                                                                                                                gCACAGGTCCGGGACAAACTCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTAc                                  <  2:3918841/150‑1 (MQ=255)
                                                                                                                 cacaGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACg                                 >  1:1399661/1‑150 (MQ=255)
                                                                                                                 cacaGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACg                                 <  2:2360005/150‑1 (MQ=255)
                                                                                                                 cacaGGTCCGGGACAAAATCTCAGCGGCTGCAACGAGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACg                                 <  2:365342/150‑1 (MQ=255)
                                                                                                                   caGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACAACGtt                               >  1:2928993/1‑150 (MQ=255)
                                                                                                                    aGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTc                              >  2:1494053/1‑150 (MQ=255)
                                                                                                                     ggTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCa                             <  1:3942100/150‑1 (MQ=255)
                                                                                                                        ccGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGa                          >  1:2005815/1‑150 (MQ=255)
                                                                                                                           ggACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAgg                       >  2:4409873/1‑150 (MQ=255)
                                                                                                                            gACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATGTGGTGAAAACTACGTTCAGGAAggg                      >  2:4575289/1‑150 (MQ=255)
                                                                                                                                aaaTCTCAGCGGCGGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAg                  <  2:1712011/150‑1 (MQ=255)
                                                                                                                                 aaTCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGa                 <  2:1461042/150‑1 (MQ=255)
                                                                                                                                   tctcAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATa               <  2:789363/150‑1 (MQ=255)
                                                                                                                                       aGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAAt           >  1:2775864/1‑150 (MQ=255)
                                                                                                                                          ggCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCg        >  1:4715045/1‑150 (MQ=255)
                                                                                                                                           gCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGc       >  2:2372786/1‑150 (MQ=255)
                                                                                                                                            cTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGcc      >  2:1710982/1‑150 (MQ=255)
                                                                                                                                            cTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGcc      <  1:4233018/150‑1 (MQ=255)
                                                                                                                                             tGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCa     <  1:2898434/150‑1 (MQ=255)
                                                                                                                                              gCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCAc    >  1:2257654/1‑150 (MQ=255)
                                                                                                                                               cAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACt   >  1:3597718/1‑150 (MQ=255)
                                                                                                                                               cAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACt   >  2:1503746/1‑150 (MQ=255)
                                                                                                                                               cAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACt   <  2:4631060/150‑1 (MQ=255)
                                                                                                                                               cAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACt   >  1:4749215/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACTT  >  CP009273/3088369‑3088662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑