Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,119,047 C→A 7.4% D279Y (GAC→TAC)  glcE ← glycolate oxidase FAD binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,119,0470CA 7.4% 1358.9 / 57.3 365D279Y (GAC→TAC) glcEglycolate oxidase FAD binding subunit
Reads supporting (aligned to +/- strand):  ref base C (174/164);  new base A (17/10);  total (191/174)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 370 total aligned reads displayed.

GTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTT  >  CP009273/3118898‑3119196
                                                                                                                                                     |                                                                                                                                                     
gTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTc                                                                                                                                                       <  2:2026094/150‑1 (MQ=255)
   aTAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                                                                                                                                                    >  1:110199/1‑150 (MQ=255)
   aTAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                                                                                                                                                    >  1:3053595/1‑150 (MQ=255)
   aTAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                                                                                                                                                    >  1:2727768/1‑150 (MQ=255)
    tAGCTGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATc                                                                                                                                                   <  2:2961652/150‑1 (MQ=255)
     aGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCATCCAGCGTAACGCCCCGCCCCAGTAGATCa                                                                                                                                                  >  1:3190238/1‑150 (MQ=255)
     aGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCa                                                                                                                                                  <  2:460022/150‑1 (MQ=255)
     aGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCa                                                                                                                                                  <  2:238543/150‑1 (MQ=255)
     aGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCa                                                                                                                                                  <  2:4594240/150‑1 (MQ=255)
           aTAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCt                                                                                                                                            <  1:4022185/150‑1 (MQ=255)
            tAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTc                                                                                                                                           <  2:1189155/150‑1 (MQ=255)
            tAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTc                                                                                                                                           >  1:2105054/1‑150 (MQ=255)
             aaaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCg                                                                                                                                          >  2:2280561/1‑150 (MQ=255)
              aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGc                                                                                                                                         >  1:2404272/1‑150 (MQ=255)
               aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGcc                                                                                                                                        >  2:4381752/1‑150 (MQ=255)
                   gCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGt                                                                                                                                    >  1:3141032/1‑150 (MQ=255)
                     cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCTTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTaa                                                                                                                                  <  1:3435807/150‑1 (MQ=255)
                     cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTaa                                                                                                                                  <  1:3104735/150‑1 (MQ=255)
                       aTAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAAt                                                                                                                                <  1:1229710/150‑1 (MQ=255)
                       aTAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAAt                                                                                                                                >  2:2849739/1‑150 (MQ=255)
                         aGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATcc                                                                                                                              >  2:1534688/1‑150 (MQ=255)
                         aGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATcc                                                                                                                              <  1:1732896/150‑1 (MQ=255)
                          gCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTCGCCGGGTAAATCca                                                                                                                             <  2:235051/150‑1 (MQ=255)
                            ggggCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatc                                                                                                                           >  1:2601120/1‑150 (MQ=255)
                             gggCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatca                                                                                                                          <  1:3028920/150‑1 (MQ=255)
                             gggCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTCGCCGGGTAAATCcatca                                                                                                                          >  2:3636815/1‑150 (MQ=255)
                              ggCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatcat                                                                                                                         <  1:2445347/150‑1 (MQ=255)
                               gCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCatcatc                                                                                                                        <  1:4277140/150‑1 (MQ=255)
                                cAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCg                                                                                                                       <  2:3286355/150‑1 (MQ=255)
                                cAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCg                                                                                                                       <  2:4185736/150‑1 (MQ=255)
                                cAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCg                                                                                                                       >  2:4800644/1‑150 (MQ=255)
                                 aaaGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCgg                                                                                                                      >  2:1926034/1‑150 (MQ=255)
                                  aaGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGgc                                                                                                                     >  1:2803021/1‑150 (MQ=255)
                                  aaGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGgc                                                                                                                     >  1:164666/1‑150 (MQ=255)
                                      caccaTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCAt                                                                                                                 >  1:2199107/1‑150 (MQ=255)
                                       accaTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATc                                                                                                                >  2:422854/1‑150 (MQ=255)
                                        ccaTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCa                                                                                                               >  1:4225289/1‑150 (MQ=255)
                                          aTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACt                                                                                                             >  1:1349452/1‑150 (MQ=255)
                                            ctcCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTgg                                                                                                           >  2:2733174/1‑150 (MQ=255)
                                               cGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTa                                                                                                        <  2:2212247/150‑1 (MQ=255)
                                                ggCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGGTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTaa                                                                                                       >  1:1026812/1‑150 (MQ=255)
                                                  cACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATg                                                                                                     <  2:3159811/150‑1 (MQ=255)
                                                  cACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATg                                                                                                     <  2:3854602/150‑1 (MQ=255)
                                                   aCTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGa                                                                                                    <  2:847722/150‑1 (MQ=255)
                                                    cTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGaa                                                                                                   >  2:3093494/1‑150 (MQ=255)
                                                    cTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGaa                                                                                                   <  2:3469365/150‑1 (MQ=255)
                                                        aGCGGGTCGCATGACCGCCAGCGTTGCGGACGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATgc                                                                                               >  1:3998379/1‑150 (MQ=255)
                                                            ggTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCa                                                                                           >  2:4356147/1‑150 (MQ=255)
                                                              tCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATa                                                                                         >  2:579122/1‑150 (MQ=255)
                                                                 cATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAgg                                                                                      <  2:618238/150‑1 (MQ=255)
                                                                  aTGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGt                                                                                     <  2:2692160/150‑1 (MQ=255)
                                                                   tGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCCTCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTa                                                                                    >  1:3676024/1‑150 (MQ=255)
                                                                   tGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCCTAAGGTa                                                                                    >  2:1468735/1‑150 (MQ=255)
                                                                      ccGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCt                                                                                 <  1:67152/150‑1 (MQ=255)
                                                                      ccGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCt                                                                                 <  1:2792264/150‑1 (MQ=255)
                                                                             cGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACg                                                                          <  1:231278/150‑1 (MQ=255)
                                                                                  cGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGagaag                                                                     <  2:1065206/150‑1 (MQ=255)
                                                                                        atgcgatgGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGc                                                               >  2:2760353/1‑150 (MQ=255)
                                                                                          gcgatgGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAg                                                             <  2:1758023/150‑1 (MQ=255)
                                                                                             atgGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttg                                                          <  2:4699717/150‑1 (MQ=255)
                                                                                               ggATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgtt                                                        <  2:4215004/150‑1 (MQ=255)
                                                                                               ggATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgtt                                                        <  1:2733182/150‑1 (MQ=255)
                                                                                                gATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttg                                                       >  1:1559786/1‑150 (MQ=255)
                                                                                                 aTTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCCTCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttgt                                                      >  1:982384/1‑150 (MQ=255)
                                                                                                 aTTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttgt                                                      <  2:2178073/150‑1 (MQ=255)
                                                                                                  tttGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCCTAAGGTACCTGGTAACGAGAAGAACGGCAgttgttgtt                                                     >  1:2963056/1‑150 (MQ=255)
                                                                                                  tttGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttgtt                                                     <  2:1648180/150‑1 (MQ=255)
                                                                                                    tGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCa                                                   <  2:3459781/150‑1 (MQ=255)
                                                                                                      aTTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACg                                                 <  2:4493493/150‑1 (MQ=255)
                                                                                                      aTTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACg                                                 >  2:731847/1‑150 (MQ=255)
                                                                                                          tCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAAt                                             <  1:2226894/150‑1 (MQ=255)
                                                                                                           ccTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAATAACGGCAGTTGTTGTTCACGCAAtt                                            >  1:3785861/1‑150 (MQ=255)
                                                                                                              cGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATtgct                                         <  1:3156007/150‑1 (MQ=255)
                                                                                                                   gTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTAGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAg                                    >  2:3309092/1‑150 (MQ=255)
                                                                                                                    tCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGa                                   >  1:642538/1‑150 (MQ=255)
                                                                                                                      gATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAAc                                 >  2:3948682/1‑150 (MQ=255)
                                                                                                                      gATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAAc                                 <  2:1981109/150‑1 (MQ=255)
                                                                                                                      gATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAAc                                 >  2:3778596/1‑150 (MQ=255)
                                                                                                                      gATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAAAGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAAc                                 >  1:2624149/1‑150 (MQ=255)
                                                                                                                       aTTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACt                                <  1:2558566/150‑1 (MQ=255)
                                                                                                                           cagccagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATCAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGAcc                            >  2:1378385/1‑150 (MQ=255)
                                                                                                                           cagccagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGAcc                            <  2:1255624/150‑1 (MQ=255)
                                                                                                                            agccagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCg                           >  1:789347/1‑150 (MQ=255)
                                                                                                                             gccagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCgg                          <  1:3362026/150‑1 (MQ=255)
                                                                                                                               cagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCa                        <  2:340327/150‑1 (MQ=255)
                                                                                                                                 gcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAAGTGCTGCCAGAACTGACCGGCAAc                      >  2:4875411/1‑150 (MQ=255)
                                                                                                                                     aaCGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACctct                  >  2:410603/1‑150 (MQ=255)
                                                                                                                                     aaCGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACctct                  <  2:170706/150‑1 (MQ=255)
                                                                                                                                       cgccccgcccCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTc                >  1:1981364/1‑150 (MQ=255)
                                                                                                                                       cgccccgcccCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTc                <  2:71604/150‑1 (MQ=255)
                                                                                                                                       cgccccgcccCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTc                <  1:3525336/150‑1 (MQ=255)
                                                                                                                                        gccccgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCg               <  2:913838/150‑1 (MQ=255)
                                                                                                                                        gccccgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCg               <  2:1877352/150‑1 (MQ=255)
                                                                                                                                           ccgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCa            <  1:2849739/150‑1 (MQ=255)
                                                                                                                                           ccgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCa            >  1:4827198/1‑150 (MQ=255)
                                                                                                                                            cgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCAc           >  1:4781203/1‑150 (MQ=255)
                                                                                                                                               cccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCca        <  1:4580204/150‑1 (MQ=255)
                                                                                                                                               cccAGTAGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCca        <  1:1537121/150‑1 (MQ=255)
                                                                                                                                                    tCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGt   <  1:4332391/150‑1 (MQ=255)
                                                                                                                                                     cGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGtt  >  2:258858/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTT  >  CP009273/3118898‑3119196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑