Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,193,298 C→A 17.2% D343Y (GAT→TAT)  ygiF ← inorganic triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,193,2980CA17.2% 1066.4 / 164.0 354D343Y (GAT→TAT) ygiFinorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base C (150/143);  new base A (27/34);  total (177/177)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 358 total aligned reads displayed.

TTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCA  >  CP009273/3193149‑3193446
                                                                                                                                                     |                                                                                                                                                    
ttGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATc                                                                                                                                                      <  1:2103759/150‑1 (MQ=255)
ttGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATc                                                                                                                                                      <  1:4835149/150‑1 (MQ=255)
  gcgcGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCgg                                                                                                                                                    <  1:1046733/150‑1 (MQ=255)
         gacAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCg                                                                                                                                             <  2:585173/150‑1 (MQ=255)
         gacAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCg                                                                                                                                             <  1:3391073/150‑1 (MQ=255)
          acAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGt                                                                                                                                            >  2:2172279/1‑150 (MQ=255)
          acAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGt                                                                                                                                            >  2:2665378/1‑150 (MQ=255)
           cAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGtt                                                                                                                                           <  1:3254155/150‑1 (MQ=255)
             ggATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTg                                                                                                                                         <  2:375783/150‑1 (MQ=255)
              gATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGa                                                                                                                                        >  1:291655/1‑150 (MQ=255)
                tCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAg                                                                                                                                      >  2:1127456/1‑150 (MQ=255)
                tCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAg                                                                                                                                      >  2:1375936/1‑150 (MQ=255)
                 cATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAgg                                                                                                                                     >  2:3456562/1‑150 (MQ=255)
                 cATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAgg                                                                                                                                     >  1:1689836/1‑150 (MQ=255)
                      tAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCg                                                                                                                                >  2:1101062/1‑150 (MQ=255)
                         cccGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTg                                                                                                                             <  2:3930770/150‑1 (MQ=255)
                         cccGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTg                                                                                                                             <  2:1473156/150‑1 (MQ=255)
                           cGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGAt                                                                                                                           >  1:4721958/1‑150 (MQ=255)
                              cAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGAtttt                                                                                                                        >  1:2968590/1‑150 (MQ=255)
                               aGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTg                                                                                                                       <  1:2704382/150‑1 (MQ=255)
                                gCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGc                                                                                                                      >  1:3210814/1‑150 (MQ=255)
                                  aaCAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGccc                                                                                                                    >  1:2636149/1‑150 (MQ=255)
                                     aGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTgc                                                                                                                 <  1:1828997/150‑1 (MQ=255)
                                     aGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTgc                                                                                                                 <  2:3570915/150‑1 (MQ=255)
                                         ttGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCtt                                                                                                             <  1:4557444/150‑1 (MQ=255)
                                          tGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCttt                                                                                                            <  1:3091990/150‑1 (MQ=255)
                                            aGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTgg                                                                                                          <  1:4191877/150‑1 (MQ=255)
                                            aGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTgg                                                                                                          >  2:2753234/1‑150 (MQ=255)
                                            aGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTgg                                                                                                          >  2:4596924/1‑150 (MQ=255)
                                               cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCGGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                       <  1:722550/150‑1 (MQ=255)
                                               cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCGGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                       <  1:4395964/150‑1 (MQ=255)
                                               cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                       <  2:1226551/150‑1 (MQ=255)
                                               cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                       >  2:1335911/1‑150 (MQ=255)
                                               cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                       >  2:4453880/1‑150 (MQ=255)
                                               cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                       <  1:1068143/150‑1 (MQ=255)
                                                aaTATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGTTTTTGCCCATCGATTTTGCaac                                                                                                      >  1:1589121/1‑148 (MQ=255)
                                                aaTATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATc                                                                                                      >  1:4827888/1‑150 (MQ=255)
                                                 atatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCAGCGCTTTGGCATCt                                                                                                     >  2:485687/1‑150 (MQ=255)
                                                  tatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTa                                                                                                    >  1:2738554/1‑150 (MQ=255)
                                                   atCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaa                                                                                                   <  1:1257562/150‑1 (MQ=255)
                                                   atCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaa                                                                                                   >  2:2529234/1‑150 (MQ=255)
                                                    tCACGCGTGAGGGGTGGCAACTGGTCACGGTAGCGATCGCGTAACGGCTTGCAGTTAACGCTTTTCAGTTCAGAGGCTTTGCGGGAATGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGTTTTTGCCCTGCGCTTTGGCATCTtaa                                                                                                  <  2:240752/150‑1 (MQ=255)
                                                       cgcgTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAAt                                                                                               <  1:3953221/150‑1 (MQ=255)
                                                       cgcgTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAAt                                                                                               >  1:3523758/1‑150 (MQ=255)
                                                         cgTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCTGCCCGTTGGGATCTaaaaaagt                                                                                             >  1:1776650/1‑147 (MQ=255)
                                                          gTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGc                                                                                            >  2:2635823/1‑150 (MQ=255)
                                                            cAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTg                                                                                          <  2:2543978/150‑1 (MQ=255)
                                                             aGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGc                                                                                         <  2:2177909/150‑1 (MQ=255)
                                                                   ggCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATTGCTGCCAtgct                                                                                   >  2:728751/1‑150 (MQ=255)
                                                                    gCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgctt                                                                                  <  2:1887561/150‑1 (MQ=255)
                                                                     cAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCAGCGCGTTTGCATATAAAAATGTCTGCCAagcatt                                                                                 >  1:129811/1‑150 (MQ=255)
                                                                      aaCTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgctttg                                                                                <  2:1777042/150‑1 (MQ=255)
                                                                           gTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATTCTTTGCTTac                                                                           >  2:841955/1‑150 (MQ=255)
                                                                            tCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTacc                                                                          <  1:117473/150‑1 (MQ=255)
                                                                            tCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTacc                                                                          <  1:1310185/150‑1 (MQ=255)
                                                                            tCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTacc                                                                          <  1:559811/150‑1 (MQ=255)
                                                                             cACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTacca                                                                         >  2:2288749/1‑150 (MQ=255)
                                                                                      gATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCgg                                                                >  2:1954840/1‑150 (MQ=255)
                                                                                      gATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCgg                                                                <  1:2198311/150‑1 (MQ=255)
                                                                                        tCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTc                                                              <  1:485986/150‑1 (MQ=255)
                                                                                        tCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTc                                                              <  2:2993973/150‑1 (MQ=255)
                                                                                        tCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCGGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTc                                                              >  2:1696648/1‑150 (MQ=255)
                                                                                         cGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCa                                                             <  2:3796650/150‑1 (MQ=255)
                                                                                             tAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCGGCGCTTTGGCATCAAAAAATTGCTGCCAAGCTTTTCTTAACAACCAATACGTCAAAGc                                                         >  1:194013/1‑150 (MQ=255)
                                                                                             tAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGc                                                         <  2:696740/150‑1 (MQ=255)
                                                                                              aaCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGcc                                                        <  1:836528/150‑1 (MQ=255)
                                                                                              aaCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCAGCGCTTTTGCATCTAAAAATTGCTGCCAAGCTTTTCTTACCAACCATTAGGTCAAAGcc                                                        >  1:999665/1‑150 (MQ=255)
                                                                                               aCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCa                                                       >  2:3551076/1‑150 (MQ=255)
                                                                                                  gCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCTTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCt                                                    <  1:4796281/150‑1 (MQ=255)
                                                                                                  gCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCt                                                    <  2:3642126/150‑1 (MQ=255)
                                                                                                   cTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCtt                                                   <  1:2222018/150‑1 (MQ=255)
                                                                                                   cTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCtt                                                   <  1:3667628/150‑1 (MQ=255)
                                                                                                    tGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTc                                                  >  2:4441332/1‑150 (MQ=255)
                                                                                                       cAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGcc                                               <  1:2297162/150‑1 (MQ=255)
                                                                                                       cAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCGTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCCACCATTCGGTCAACGCCAGCGTCGcc                                               >  2:4634965/1‑150 (MQ=255)
                                                                                                       cAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGAGTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGcc                                               >  2:4053388/1‑150 (MQ=255)
                                                                                                        aGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAAAGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCa                                              >  2:2900211/1‑150 (MQ=255)
                                                                                                        aGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCAGCGCGTTTGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCAATCGGTCAACCCCACCGTCGCCa                                              >  1:1428037/1‑150 (MQ=255)
                                                                                                        aGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCa                                              <  2:2138331/150‑1 (MQ=255)
                                                                                                            aaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCTGCGCATTTGCATCTAAAAATTGCTGCCATTGTTTTGTTACCAACCAATCGGTCAACCCCACCTTAGCCAATGc                                          >  1:37670/1‑150 (MQ=255)
                                                                                                             aCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGcc                                         <  1:1999223/150‑1 (MQ=255)
                                                                                                                 ttttCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGttt                                     <  1:3573155/150‑1 (MQ=255)
                                                                                                                     cagttcagCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgt                                 <  2:2974778/150‑1 (MQ=255)
                                                                                                                     cagttcagCGGCATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgt                                 >  2:437109/1‑150 (MQ=255)
                                                                                                                           agCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAACTAAAAATGGCTGCCATGCGTTGCTTACCAAACAATAGGTCAACGCCAGCTTCGCCATTGCCGGTTCGgtagagta                           >  1:1386098/1‑150 (MQ=255)
                                                                                                                             cGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgtagagtaga                         <  1:3328409/150‑1 (MQ=255)
                                                                                                                             cGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgtagagtaga                         <  1:2172279/150‑1 (MQ=255)
                                                                                                                             cGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCAGCGCTTTGGCATCGAAAAATTGCTGCCATGCTTTGCTTACCAAACAATCGGTAAACGCCACCTTCGCCAATTCCGTTTAGggagagtaga                         >  1:3754312/1‑150 (MQ=255)
                                                                                                                                cATGGCGGGAAAGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTAGGTCAACGCCAGCTTCGCCCTTGCCGTGTCGGTAGTGTAGAccg                      >  2:4155476/1‑150 (MQ=255)
                                                                                                                                     cGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTc                 >  2:4815088/1‑150 (MQ=255)
                                                                                                                                     cGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTc                 <  2:2051068/150‑1 (MQ=255)
                                                                                                                                     cGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCAATGCCGTTTCGGTAGAGTAGACCGCCGTc                 >  2:4733604/1‑150 (MQ=255)
                                                                                                                                      gggAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGGTTCGGTAGAGTAGACCGCCGTCa                >  2:2719917/1‑150 (MQ=255)
                                                                                                                                         aaaGATGGATATAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGGCACgg             >  2:3969270/1‑150 (MQ=255)
                                                                                                                                          aaGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGc            >  2:281601/1‑150 (MQ=255)
                                                                                                                                           aGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCa           <  2:4762205/150‑1 (MQ=255)
                                                                                                                                           aGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCa           <  2:3750435/150‑1 (MQ=255)
                                                                                                                                              tGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCGTCGCCCTTGCCGTTTCGGGAGAGTAGACCGCCGTCACGGCAGac        >  2:4747601/1‑150 (MQ=255)
                                                                                                                                                 atatAGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACAcc     <  1:1615767/150‑1 (MQ=255)
                                                                                                                                                  tatCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCg    <  2:1644882/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCA  >  CP009273/3193149‑3193446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑